CA1114271A - Dosage de bacteries gram negatives - Google Patents

Dosage de bacteries gram negatives

Info

Publication number: CA1114271A
Authority: CA; Canada
Prior art keywords: bacteria; active agent; gram; liquid phase; fluid
Prior art date: 1978-04-20
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired

Application number

CA325,901A

Other languages

English (en)

Inventor

Gary C. Du Moulin

John Hedley-Whyte

Susan E. Lynch

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Harvard University

Original Assignee

Harvard University

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1978-04-20

Filing date

1979-04-19

Publication date

1981-12-15

1979-04-19 Application filed by Harvard University filed Critical Harvard University

1981-12-15 Application granted granted Critical

1981-12-15 Publication of CA1114271A publication Critical patent/CA1114271A/fr

Status Expired legal-status Critical Current

Links

241000894006 Bacteria Species 0.000 title claims abstract description 33
238000003556 assay Methods 0.000 title claims abstract description 11
238000000034 method Methods 0.000 claims abstract description 22
239000013060 biological fluid Substances 0.000 claims abstract description 21
239000007791 liquid phase Substances 0.000 claims abstract description 14
239000006166 lysate Substances 0.000 claims abstract description 11
241000239220 Limulus polyphemus Species 0.000 claims abstract description 8
230000001413 cellular effect Effects 0.000 claims abstract description 8
239000012530 fluid Substances 0.000 claims abstract description 7
230000002934 lysing effect Effects 0.000 claims abstract description 6
239000004094 surface-active agent Substances 0.000 claims description 15
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 11
210000000601 blood cell Anatomy 0.000 claims description 8
238000005119 centrifugation Methods 0.000 claims description 7
210000004369 blood Anatomy 0.000 claims description 6
239000008280 blood Substances 0.000 claims description 6
238000004062 sedimentation Methods 0.000 claims description 6
238000011534 incubation Methods 0.000 claims description 5
239000007788 liquid Substances 0.000 claims description 5
238000002156 mixing Methods 0.000 claims description 5
238000010790 dilution Methods 0.000 claims description 4
239000012895 dilution Substances 0.000 claims description 4
238000001914 filtration Methods 0.000 claims description 4
239000002510 pyrogen Substances 0.000 claims description 4
238000000926 separation method Methods 0.000 claims description 4
238000005406 washing Methods 0.000 claims description 4
244000005700 microbiome Species 0.000 abstract description 13
239000012736 aqueous medium Substances 0.000 abstract description 2
239000002158 endotoxin Substances 0.000 description 10
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 9
230000003287 optical effect Effects 0.000 description 8
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 8
239000000243 solution Substances 0.000 description 7
239000011780 sodium chloride Substances 0.000 description 6
239000008188 pellet Substances 0.000 description 4
239000006228 supernatant Substances 0.000 description 4
206010003504 Aspiration Diseases 0.000 description 3
208000031729 Bacteremia Diseases 0.000 description 3
241000588724 Escherichia coli Species 0.000 description 3
239000011521 glass Substances 0.000 description 3
239000011148 porous material Substances 0.000 description 3
239000000725 suspension Substances 0.000 description 3
239000002028 Biomass Substances 0.000 description 2
208000035473 Communicable disease Diseases 0.000 description 2
241001302191 Polyphemus Species 0.000 description 2
239000013504 Triton X-100 Substances 0.000 description 2
229920004890 Triton X-100 Polymers 0.000 description 2
239000007864 aqueous solution Substances 0.000 description 2
210000004027 cell Anatomy 0.000 description 2
HDFXRQJQZBPDLF-UHFFFAOYSA-L disodium hydrogen carbonate Chemical compound [Na+].[Na+].OC([O-])=O.OC([O-])=O HDFXRQJQZBPDLF-UHFFFAOYSA-L 0.000 description 2
150000002500 ions Chemical class 0.000 description 2
-1 iso-octylphenoxy Chemical group 0.000 description 2
230000035945 sensitivity Effects 0.000 description 2
239000012086 standard solution Substances 0.000 description 2
206010003445 Ascites Diseases 0.000 description 1
241000606125 Bacteroides Species 0.000 description 1
241000606124 Bacteroides fragilis Species 0.000 description 1
IAYPIBMASNFSPL-UHFFFAOYSA-N Ethylene oxide Chemical compound C1CO1 IAYPIBMASNFSPL-UHFFFAOYSA-N 0.000 description 1
241000588748 Klebsiella Species 0.000 description 1
239000012480 LAL reagent Substances 0.000 description 1
206010035664 Pneumonia Diseases 0.000 description 1
GOOHAUXETOMSMM-UHFFFAOYSA-N Propylene oxide Chemical compound CC1CO1 GOOHAUXETOMSMM-UHFFFAOYSA-N 0.000 description 1
206010040047 Sepsis Diseases 0.000 description 1
230000000118 anti-neoplastic effect Effects 0.000 description 1
229940034982 antineoplastic agent Drugs 0.000 description 1
238000001660 aspiration Methods 0.000 description 1
230000015572 biosynthetic process Effects 0.000 description 1
238000009640 blood culture Methods 0.000 description 1
239000000872 buffer Substances 0.000 description 1
210000001175 cerebrospinal fluid Anatomy 0.000 description 1
239000003795 chemical substances by application Substances 0.000 description 1
150000001875 compounds Chemical class 0.000 description 1
238000012937 correction Methods 0.000 description 1
230000009089 cytolysis Effects 0.000 description 1
238000005202 decontamination Methods 0.000 description 1
230000003588 decontaminative effect Effects 0.000 description 1
238000003745 diagnosis Methods 0.000 description 1
208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
208000035475 disorder Diseases 0.000 description 1
239000006185 dispersion Substances 0.000 description 1
230000007613 environmental effect Effects 0.000 description 1
239000012634 fragment Substances 0.000 description 1
238000001879 gelation Methods 0.000 description 1
230000002209 hydrophobic effect Effects 0.000 description 1
230000009545 invasion Effects 0.000 description 1
101150085091 lat-2 gene Proteins 0.000 description 1
239000000203 mixture Substances 0.000 description 1
UYDLBVPAAFVANX-UHFFFAOYSA-N octylphenoxy polyethoxyethanol Chemical compound CC(C)(C)CC(C)(C)C1=CC=C(OCCOCCOCCOCCO)C=C1 UYDLBVPAAFVANX-UHFFFAOYSA-N 0.000 description 1
229920003023 plastic Polymers 0.000 description 1
230000002980 postoperative effect Effects 0.000 description 1
239000002244 precipitate Substances 0.000 description 1
239000002243 precursor Substances 0.000 description 1
230000001698 pyrogenic effect Effects 0.000 description 1
239000013535 sea water Substances 0.000 description 1
239000007787 solid Substances 0.000 description 1
238000011282 treatment Methods 0.000 description 1
210000002700 urine Anatomy 0.000 description 1
238000003828 vacuum filtration Methods 0.000 description 1
239000002699 waste material Substances 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor

Landscapes

Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Health & Medical Sciences (AREA)
Organic Chemistry (AREA)
Engineering & Computer Science (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Analytical Chemistry (AREA)
Toxicology (AREA)
Immunology (AREA)
Microbiology (AREA)
Molecular Biology (AREA)
Biophysics (AREA)
Physics & Mathematics (AREA)
Biotechnology (AREA)
Biochemistry (AREA)
Bioinformatics & Cheminformatics (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

CA325,901A 1978-04-20 1979-04-19 Dosage de bacteries gram negatives Expired CA1114271A (fr)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US89829178A	1978-04-20	1978-04-20
US898,291		1978-04-20

Publications (1)

Publication Number	Publication Date
CA1114271A true CA1114271A (fr)	1981-12-15

Family

ID=25409227

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CA325,901A Expired CA1114271A (fr)	1978-04-20	1979-04-19	Dosage de bacteries gram negatives

Country Status (9)

Country	Link
JP (1)	JPS54149699A (fr)
BE (1)	BE875716A (fr)
CA (1)	CA1114271A (fr)
CH (1)	CH641493A5 (fr)
DE (1)	DE2915569A1 (fr)
FR (1)	FR2423784A1 (fr)
GB (1)	GB2019563B (fr)
IT (1)	IT1119718B (fr)
NL (1)	NL7902983A (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US4322217A (en)	1980-06-27	1982-03-30	Mallinckrodt, Inc.	Process for preparing Limulus lysate
US5594113A (en) *	1988-06-23	1997-01-14	Associates Of Cape Cod, Inc.	Endotoxin binding and neutralizing protein and uses thereof
EP3192876A1 (fr) *	2007-10-10	2017-07-19	Pocared Diagnostics Ltd.	Système permettant d'effectuer l'identification de bactéries dans l'urine
US8519358B2 (en)	2008-02-05	2013-08-27	Pocared Diagnostics Ltd.	System for conducting the identification of bacteria in biological samples
US10288632B2 (en)	2009-09-21	2019-05-14	Pocared Diagnostics Ltd.	System for conducting the identification of bacteria in biological samples
GB2477752A (en) *	2010-02-11	2011-08-17	Arab Biotechnology Company	Detection of bacteria
WO2014093463A1 (fr)	2012-12-11	2014-06-19	Pocared Diagnostics Ltd.	Cuvette optique à fond incurvé

1979
- 1979-04-03 GB GB7911610A patent/GB2019563B/en not_active Expired
- 1979-04-12 IT IT21820/79A patent/IT1119718B/it active
- 1979-04-17 NL NL7902983A patent/NL7902983A/xx not_active Application Discontinuation
- 1979-04-18 DE DE19792915569 patent/DE2915569A1/de not_active Withdrawn
- 1979-04-19 BE BE0/194719A patent/BE875716A/fr not_active IP Right Cessation
- 1979-04-19 CA CA325,901A patent/CA1114271A/fr not_active Expired
- 1979-04-20 FR FR7910134A patent/FR2423784A1/fr active Granted
- 1979-04-20 CH CH377279A patent/CH641493A5/de not_active IP Right Cessation
- 1979-04-20 JP JP4888479A patent/JPS54149699A/ja active Pending

Also Published As

Publication number	Publication date
IT1119718B (it)	1986-03-10
JPS54149699A (en)	1979-11-24
IT7921820A0 (it)	1979-04-12
NL7902983A (nl)	1979-10-23
BE875716A (fr)	1979-08-16
CH641493A5 (de)	1984-02-29
GB2019563A (en)	1979-10-31
FR2423784A1 (fr)	1979-11-16
DE2915569A1 (de)	1979-11-08
GB2019563B (en)	1982-07-28
FR2423784B3 (fr)	1982-02-26

Legal Events

Date	Code	Title	Description
1998-12-15	MKEX	Expiry

Publication	Publication Date	Title
Herbert	1990	1 Methods for Enumerating Microorganisms and Determining Biomass in Natural Environments
Berthet et al.	1951	Tissue fractionation studies. 1. The existence of a mitochondria-linked, enzymically inactive form of acid phosphatase in rat-liver tissue
Paul et al.	1982	Fluorometric determination of DNA in aquatic microorganisms by use of Hoechst 33258
AU700699B2 (en)	1999-01-14	Preparation and stabilisation of cells
Müller et al.	1981	Lectin, a possible basis for symbiosis between bacteria and sponges
Lucio et al.	1990	Identification of the chicken anemia agent, reproduction of the disease, and serological survey in the United States
Wilkinson	1987	Hospital-laboratory diagnosis of Legionella infections
Krieger et al.	1985	Geographic variation among isolates of Trichomonas vaginalis: demonstration of antigenic heterogeneity by using monoclonal antibodies and the indirect immunofluorescence technique
Vávra et al.	1993	Staining of microsporidian spores by optical brighteners with remarks on the use of brighteners for the diagnosis of AIDS associated human
Vermunt et al.	1992	Isolation of salmonellas by immunomagnetic separation
EP0419502B1 (fr)	1996-01-17	Procede de detection de bacteries dans l'urine
Miörner et al.	1996	Improved sensitivity of direct microscopy for acid-fast bacilli: sedimentation as an alternative to centrifugation for concentration of tubercle bacilli
CA1114271A (fr)	1981-12-15	Dosage de bacteries gram negatives
NZ332732A (en)	1999-09-29	Specimen collection fluid comprising a sterile buffered aqueous solution of an aliphatic aldehyde and heavy metal salts
Berger et al.	1979	Colorimetric enzyme-linked immunosorbent assay for the identification of strains of Rhizobium in culture and in the nodules of lentils
Gately et al.	1974	The molecular dimensions of guinea pig lymphotoxin
Bolann et al.	1989	Implications of probability analysis for interpreting results of leukocyte esterase and nitrite test strips.
Crawford	1969	Purification of polyoma virus
JPS61501226A (ja)	1986-06-19	生体分子と生物細胞の濃縮及び検出方法並びにその装置
Walter et al.	1995	Differential electrophoretic behavior in aqueous polymer solutions of red blood cells from Alzheimer patients and from normal individuals
Mansour et al.	1984	Fluorescent staining of intracellular and extracellular bacteria in blood
Hazen et al.	1990	Improved assay for surface hydrophobic avidity of Candida albicans cells
Padh	1992	Organelle isolation and marker enzyme assay
JPH0829426A (ja)	1996-02-02	硝酸菌、亜硝酸菌の検出用抗体感作ラテックス
CN112730414A (zh)	2021-04-30	一种高效检测水中贾第鞭毛虫和隐孢子虫的方法