CH641493A5 - Verfahren zur bestimmung von gram-negativen bakterien. - Google Patents

Verfahren zur bestimmung von gram-negativen bakterien. Download PDF

Info

Publication number: CH641493A5
Authority: CH; Switzerland
Prior art keywords: microorganisms; liquid; liquid phase; pyrogen; negative bacteria
Prior art date: 1978-04-20

Application number

CH377279A

Other languages

German (de)

English (en)

Inventor

Gary C Du Moulin

Susan E Lynch

John Hedley-Whyte

Original Assignee

Harvard College

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1978-04-20

Filing date

1979-04-20

Publication date

1984-02-29

1979-04-20 Application filed by Harvard College filed Critical Harvard College

1984-02-29 Publication of CH641493A5 publication Critical patent/CH641493A5/de

Links

238000000034 method Methods 0.000 title claims description 20
241000894006 Bacteria Species 0.000 title claims description 17
244000005700 microbiome Species 0.000 claims description 23
239000007788 liquid Substances 0.000 claims description 20
239000007791 liquid phase Substances 0.000 claims description 13
239000004094 surface-active agent Substances 0.000 claims description 12
239000006166 lysate Substances 0.000 claims description 11
241000239220 Limulus polyphemus Species 0.000 claims description 9
210000004369 blood Anatomy 0.000 claims description 6
239000008280 blood Substances 0.000 claims description 6
238000005119 centrifugation Methods 0.000 claims description 6
238000004062 sedimentation Methods 0.000 claims description 6
238000001914 filtration Methods 0.000 claims description 5
239000002510 pyrogen Substances 0.000 claims description 3
238000000926 separation method Methods 0.000 claims description 3
238000005406 washing Methods 0.000 claims description 2
239000003085 diluting agent Substances 0.000 claims 1
238000012360 testing method Methods 0.000 description 12
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 10
239000002158 endotoxin Substances 0.000 description 10
230000003287 optical effect Effects 0.000 description 8
239000000523 sample Substances 0.000 description 8
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 8
239000013060 biological fluid Substances 0.000 description 7
210000004027 cell Anatomy 0.000 description 7
239000000243 solution Substances 0.000 description 7
239000002244 precipitate Substances 0.000 description 5
239000006228 supernatant Substances 0.000 description 5
241000588724 Escherichia coli Species 0.000 description 4
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
238000011534 incubation Methods 0.000 description 4
239000000725 suspension Substances 0.000 description 4
239000007864 aqueous solution Substances 0.000 description 3
239000011148 porous material Substances 0.000 description 3
239000011780 sodium chloride Substances 0.000 description 3
208000031729 Bacteremia Diseases 0.000 description 2
239000002028 Biomass Substances 0.000 description 2
208000035473 Communicable disease Diseases 0.000 description 2
239000013504 Triton X-100 Substances 0.000 description 2
229920004890 Triton X-100 Polymers 0.000 description 2
230000001580 bacterial effect Effects 0.000 description 2
210000000601 blood cell Anatomy 0.000 description 2
238000011088 calibration curve Methods 0.000 description 2
239000003795 chemical substances by application Substances 0.000 description 2
238000010790 dilution Methods 0.000 description 2
239000012895 dilution Substances 0.000 description 2
HDFXRQJQZBPDLF-UHFFFAOYSA-L disodium hydrogen carbonate Chemical compound [Na+].[Na+].OC([O-])=O.OC([O-])=O HDFXRQJQZBPDLF-UHFFFAOYSA-L 0.000 description 2
238000004090 dissolution Methods 0.000 description 2
230000007613 environmental effect Effects 0.000 description 2
239000012530 fluid Substances 0.000 description 2
239000011521 glass Substances 0.000 description 2
238000012454 limulus amebocyte lysate test Methods 0.000 description 2
230000002934 lysing effect Effects 0.000 description 2
239000002245 particle Substances 0.000 description 2
230000035945 sensitivity Effects 0.000 description 2
239000012086 standard solution Substances 0.000 description 2
206010003445 Ascites Diseases 0.000 description 1
241000606125 Bacteroides Species 0.000 description 1
241000606124 Bacteroides fragilis Species 0.000 description 1
241000588722 Escherichia Species 0.000 description 1
IAYPIBMASNFSPL-UHFFFAOYSA-N Ethylene oxide Chemical compound C1CO1 IAYPIBMASNFSPL-UHFFFAOYSA-N 0.000 description 1
241000588748 Klebsiella Species 0.000 description 1
241000588747 Klebsiella pneumoniae Species 0.000 description 1
241000239218 Limulus Species 0.000 description 1
241001302191 Polyphemus Species 0.000 description 1
GOOHAUXETOMSMM-UHFFFAOYSA-N Propylene oxide Chemical compound CC1CO1 GOOHAUXETOMSMM-UHFFFAOYSA-N 0.000 description 1
206010040047 Sepsis Diseases 0.000 description 1
210000000683 abdominal cavity Anatomy 0.000 description 1
239000002253 acid Substances 0.000 description 1
150000007513 acids Chemical class 0.000 description 1
XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
229910052782 aluminium Inorganic materials 0.000 description 1
239000003708 ampul Substances 0.000 description 1
238000003556 assay Methods 0.000 description 1
239000012472 biological sample Substances 0.000 description 1
230000015572 biosynthetic process Effects 0.000 description 1
238000009640 blood culture Methods 0.000 description 1
210000004556 brain Anatomy 0.000 description 1
239000000872 buffer Substances 0.000 description 1
210000001175 cerebrospinal fluid Anatomy 0.000 description 1
230000000052 comparative effect Effects 0.000 description 1
150000001875 compounds Chemical class 0.000 description 1
230000007547 defect Effects 0.000 description 1
238000003745 diagnosis Methods 0.000 description 1
239000000706 filtrate Substances 0.000 description 1
239000012634 fragment Substances 0.000 description 1
230000005484 gravity Effects 0.000 description 1
230000002209 hydrophobic effect Effects 0.000 description 1
210000000987 immune system Anatomy 0.000 description 1
239000004615 ingredient Substances 0.000 description 1
238000011835 investigation Methods 0.000 description 1
229920006008 lipopolysaccharide Polymers 0.000 description 1
239000000203 mixture Substances 0.000 description 1
UYDLBVPAAFVANX-UHFFFAOYSA-N octylphenoxy polyethoxyethanol Chemical compound CC(C)(C)CC(C)(C)C1=CC=C(OCCOCCOCCOCCO)C=C1 UYDLBVPAAFVANX-UHFFFAOYSA-N 0.000 description 1
239000008188 pellet Substances 0.000 description 1
230000035515 penetration Effects 0.000 description 1
229920003023 plastic Polymers 0.000 description 1
230000002980 postoperative effect Effects 0.000 description 1
239000002243 precursor Substances 0.000 description 1
230000001698 pyrogenic effect Effects 0.000 description 1
239000013535 sea water Substances 0.000 description 1
239000013049 sediment Substances 0.000 description 1
239000007787 solid Substances 0.000 description 1
210000000278 spinal cord Anatomy 0.000 description 1
238000004659 sterilization and disinfection Methods 0.000 description 1
239000011550 stock solution Substances 0.000 description 1
210000001519 tissue Anatomy 0.000 description 1
210000002700 urine Anatomy 0.000 description 1
238000003828 vacuum filtration Methods 0.000 description 1
210000003462 vein Anatomy 0.000 description 1
239000002699 waste material Substances 0.000 description 1
239000008207 working material Substances 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor

Landscapes

Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Health & Medical Sciences (AREA)
Organic Chemistry (AREA)
Engineering & Computer Science (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Analytical Chemistry (AREA)
Toxicology (AREA)
Immunology (AREA)
Microbiology (AREA)
Molecular Biology (AREA)
Biophysics (AREA)
Physics & Mathematics (AREA)
Biotechnology (AREA)
Biochemistry (AREA)
Bioinformatics & Cheminformatics (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

CH377279A 1978-04-20 1979-04-20 Verfahren zur bestimmung von gram-negativen bakterien. CH641493A5 (de)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
US89829178A	1978-04-20	1978-04-20

Publications (1)

Publication Number	Publication Date
CH641493A5 true CH641493A5 (de)	1984-02-29

Family

ID=25409227

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CH377279A CH641493A5 (de)	1978-04-20	1979-04-20	Verfahren zur bestimmung von gram-negativen bakterien.

Country Status (9)

Country	Link
JP (1)	JPS54149699A (fr)
BE (1)	BE875716A (fr)
CA (1)	CA1114271A (fr)
CH (1)	CH641493A5 (fr)
DE (1)	DE2915569A1 (fr)
FR (1)	FR2423784A1 (fr)
GB (1)	GB2019563B (fr)
IT (1)	IT1119718B (fr)
NL (1)	NL7902983A (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US4322217A (en)	1980-06-27	1982-03-30	Mallinckrodt, Inc.	Process for preparing Limulus lysate
US5594113A (en) *	1988-06-23	1997-01-14	Associates Of Cape Cod, Inc.	Endotoxin binding and neutralizing protein and uses thereof
EP3192876A1 (fr) *	2007-10-10	2017-07-19	Pocared Diagnostics Ltd.	Système permettant d'effectuer l'identification de bactéries dans l'urine
US8519358B2 (en)	2008-02-05	2013-08-27	Pocared Diagnostics Ltd.	System for conducting the identification of bacteria in biological samples
US10288632B2 (en)	2009-09-21	2019-05-14	Pocared Diagnostics Ltd.	System for conducting the identification of bacteria in biological samples
GB2477752A (en) *	2010-02-11	2011-08-17	Arab Biotechnology Company	Detection of bacteria
WO2014093463A1 (fr)	2012-12-11	2014-06-19	Pocared Diagnostics Ltd.	Cuvette optique à fond incurvé

1979
- 1979-04-03 GB GB7911610A patent/GB2019563B/en not_active Expired
- 1979-04-12 IT IT21820/79A patent/IT1119718B/it active
- 1979-04-17 NL NL7902983A patent/NL7902983A/xx not_active Application Discontinuation
- 1979-04-18 DE DE19792915569 patent/DE2915569A1/de not_active Withdrawn
- 1979-04-19 BE BE0/194719A patent/BE875716A/fr not_active IP Right Cessation
- 1979-04-19 CA CA325,901A patent/CA1114271A/fr not_active Expired
- 1979-04-20 FR FR7910134A patent/FR2423784A1/fr active Granted
- 1979-04-20 CH CH377279A patent/CH641493A5/de not_active IP Right Cessation
- 1979-04-20 JP JP4888479A patent/JPS54149699A/ja active Pending

Also Published As

Publication number	Publication date
IT1119718B (it)	1986-03-10
JPS54149699A (en)	1979-11-24
IT7921820A0 (it)	1979-04-12
NL7902983A (nl)	1979-10-23
BE875716A (fr)	1979-08-16
GB2019563A (en)	1979-10-31
FR2423784A1 (fr)	1979-11-16
DE2915569A1 (de)	1979-11-08
CA1114271A (fr)	1981-12-15
GB2019563B (en)	1982-07-28
FR2423784B3 (fr)	1982-02-26

Legal Events

Date	Code	Title	Description
1987-12-31	PL	Patent ceased
2024-10-31	PL	Patent ceased

Publication	Publication Date	Title
DE2365629C2 (de)	1983-06-16	Verfahren zur Herstellung von Limulina-Lysat oder dessen gefriergetrocknetem Pulver
DE69432751T2 (de)	2004-04-29	Präparation und stabilisation von zellen
DE69603255T2 (de)	2000-04-06	Verfahren zur modifizierung von oberflächen
DE69504646T2 (de)	1999-04-29	Herstellung und stabilisierung von zellsuspensionen
DE69716064T2 (de)	2004-01-08	Flüssigkeit zum sammeln von proben
DD202178A5 (de)	1983-08-31	Verfahren und reagens zur untersuchung von antigen-antikoerper-reaktionen
DE2121994C3 (de)	1982-06-03	Ermitteln der Empfindlichkeit von Bakterienstämmen auf antibiotische und/oder chemotherapeutische Mittel
DE2248475B2 (de)	1978-02-23	Verfahren zur gewinnung von hepatitissicheren, lagerungsstabilen und infundierbaren haemoglobinloesungen
CH641493A5 (de)	1984-02-29	Verfahren zur bestimmung von gram-negativen bakterien.
DE2134928A1 (de)	1972-01-27	Biologisches Reagens
DE2602997C2 (de)	1983-05-11	Lyophilisierte Kollagenzubereitung und Verfahren zu ihrer Herstellung
DE69123445T2 (de)	1997-04-24	Verfahren zur bestimmung von endotoxinen
DE2951672C2 (fr)	1987-10-08
DE2833545A1 (de)	1979-02-15	Hochmolekulare meningokokken- gruppe c-vaccine und verfahren zu ihrer herstellung
DE69731103T2 (de)	2005-11-17	Verfahren zur proteinextraktion
DE3151337A1 (de)	1982-07-29	Blutkoagulationspromotor und verfahren zur beschleunigung der koagulation von gesamtblut
DE602005003394T2 (de)	2008-09-25	Verfahren zur Abtrennung und Inaktivierung von Virus- und Prionenkontaminationen
DE3781279T2 (de)	1993-01-07	Verfahren zur behandlung von blut.
DE3404821A1 (de)	1985-08-14	Verfahren zum nachweis von allergenhaltigem hausstaub
DE3344607A1 (de)	1984-06-14	Verfahren und zusammensetzung fuer die bestimmung der phagocytischen reaktion
DE2412137A1 (de)	1974-10-03	Praeparat zur verwendung bei blutuntersuchungsverfahren
DE2038722A1 (de)	1971-02-18	Verfahren zum Abtennen von Plasma oder Serum aus Blut
DE2517860B2 (de)	1979-04-19	Verfahren zum Bestimmen des Pyrogengehalts in Proben
DE2353035B2 (de)	1979-10-31	Verfahren zur Stabilisierung menschlicher oder tierischer Körperflüssigkeiten oder pflanzlicher Extrakte
DE2429231A1 (de)	1975-01-09	Diagnostikum fuer treponema-bakterien