CA1127536A - Procede d'identification des infections a virus au moyen d'anticorps et de bacteries - Google Patents
Procede d'identification des infections a virus au moyen d'anticorps et de bacteriesInfo
- Publication number
- CA1127536A CA1127536A CA337,922A CA337922A CA1127536A CA 1127536 A CA1127536 A CA 1127536A CA 337922 A CA337922 A CA 337922A CA 1127536 A CA1127536 A CA 1127536A
- Authority
- CA
- Canada
- Prior art keywords
- antibody
- bacteria
- cells
- sample
- bound
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 241000894006 Bacteria Species 0.000 title claims abstract description 43
- 230000003612 virological effect Effects 0.000 title description 6
- 208000015181 infectious disease Diseases 0.000 title description 5
- 210000004027 cell Anatomy 0.000 claims abstract description 64
- 241000700605 Viruses Species 0.000 claims abstract description 29
- 238000001727 in vivo Methods 0.000 claims abstract description 15
- 208000036142 Viral infection Diseases 0.000 claims abstract description 14
- 230000009385 viral infection Effects 0.000 claims abstract description 14
- 239000000523 sample Substances 0.000 claims description 26
- 239000013068 control sample Substances 0.000 claims description 21
- 238000000034 method Methods 0.000 claims description 18
- 108060003951 Immunoglobulin Proteins 0.000 claims description 9
- 102000018358 immunoglobulin Human genes 0.000 claims description 9
- 241000191967 Staphylococcus aureus Species 0.000 claims description 8
- XQFRJNBWHJMXHO-RRKCRQDMSA-N IDUR Chemical compound C1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C(I)=C1 XQFRJNBWHJMXHO-RRKCRQDMSA-N 0.000 claims description 3
- 208000002606 Paramyxoviridae Infections Diseases 0.000 claims description 3
- 239000012678 infectious agent Substances 0.000 claims description 3
- 230000000241 respiratory effect Effects 0.000 claims description 3
- 210000002345 respiratory system Anatomy 0.000 claims description 3
- 208000003265 stomatitis Diseases 0.000 claims description 3
- 208000005925 vesicular stomatitis Diseases 0.000 claims description 3
- 230000000007 visual effect Effects 0.000 claims description 3
- 239000011541 reaction mixture Substances 0.000 claims 1
- 238000011534 incubation Methods 0.000 abstract description 9
- 230000036046 immunoreaction Effects 0.000 abstract description 2
- 238000000926 separation method Methods 0.000 abstract description 2
- 210000002966 serum Anatomy 0.000 description 7
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 5
- 241001465754 Metazoa Species 0.000 description 4
- 241000283973 Oryctolagus cuniculus Species 0.000 description 4
- 239000000872 buffer Substances 0.000 description 4
- 238000003745 diagnosis Methods 0.000 description 4
- 238000010790 dilution Methods 0.000 description 4
- 239000012895 dilution Substances 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 241000699800 Cricetinae Species 0.000 description 3
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 3
- 239000006285 cell suspension Substances 0.000 description 3
- 238000001035 drying Methods 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- 102000018697 Membrane Proteins Human genes 0.000 description 2
- 108010052285 Membrane Proteins Proteins 0.000 description 2
- 230000000569 anti-influenzal effect Effects 0.000 description 2
- 239000000834 fixative Substances 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 210000005260 human cell Anatomy 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 102000019260 B-Cell Antigen Receptors Human genes 0.000 description 1
- 108010012919 B-Cell Antigen Receptors Proteins 0.000 description 1
- 241000282693 Cercopithecidae Species 0.000 description 1
- 241001517310 Eria Species 0.000 description 1
- 102000006395 Globulins Human genes 0.000 description 1
- 108010044091 Globulins Proteins 0.000 description 1
- 208000009889 Herpes Simplex Diseases 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 241000725643 Respiratory syncytial virus Species 0.000 description 1
- 241000700584 Simplexvirus Species 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 241000711975 Vesicular stomatitis virus Species 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 239000013592 cell lysate Substances 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 229940072221 immunoglobulins Drugs 0.000 description 1
- 238000001114 immunoprecipitation Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000002458 infectious effect Effects 0.000 description 1
- 210000003292 kidney cell Anatomy 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- WSFSSNUMVMOOMR-NJFSPNSNSA-N methanone Chemical compound O=[14CH2] WSFSSNUMVMOOMR-NJFSPNSNSA-N 0.000 description 1
- 238000006386 neutralization reaction Methods 0.000 description 1
- 238000002135 phase contrast microscopy Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000007790 scraping Methods 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000004114 suspension culture Methods 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 241000712461 unidentified influenza virus Species 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56983—Viruses
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/585—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with a particulate label, e.g. coloured latex
- G01N33/586—Liposomes, microcapsules or cells
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Virology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- Tropical Medicine & Parasitology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US95271978A | 1978-10-19 | 1978-10-19 | |
| US952,719 | 1978-10-19 | ||
| US7943879A | 1979-09-27 | 1979-09-27 | |
| US79,438 | 1979-09-27 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1127536A true CA1127536A (fr) | 1982-07-13 |
Family
ID=26762008
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA337,922A Expired CA1127536A (fr) | 1978-10-19 | 1979-10-18 | Procede d'identification des infections a virus au moyen d'anticorps et de bacteries |
Country Status (5)
| Country | Link |
|---|---|
| CA (1) | CA1127536A (fr) |
| DE (1) | DE2941575A1 (fr) |
| FR (1) | FR2439233A1 (fr) |
| GB (1) | GB2034464B (fr) |
| SE (1) | SE7908656L (fr) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IL66733A (en) * | 1982-09-07 | 1986-03-31 | Yeda Res & Dev | Assay for ifn and kit therefor |
| ATE36604T1 (de) * | 1983-10-26 | 1988-09-15 | Lembke Juergen | Neues verfahren zum virus-nachweis in reinkulturen mikroskopischer kleinlebewesen sowie in lebensmitteln und in technologischen produkten. |
| SE466623B (sv) * | 1989-06-01 | 1992-03-09 | Karobio Ab | Saett och testsats foer diagnosticering av en aktiv infektion |
| GB8919230D0 (en) * | 1989-08-24 | 1989-10-04 | Vaccine Research Foundation Li | Method for in vivo testing of immunity in non-human subjects |
| CN114384244B (zh) * | 2021-11-11 | 2024-12-10 | 北京英诺特生物技术股份有限公司 | 直接免疫荧光检测试剂盒的制备方法、试剂盒及联检方法 |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1562804A (en) * | 1972-06-26 | 1980-03-19 | Gen Electric | Detection of antibodies and antigens |
| DE2322562C2 (de) * | 1972-11-06 | 1984-03-29 | Pharmacia AB, Uppsala | Verfahren zur Bestimmung von Antigenen in einer Probe |
-
1979
- 1979-10-13 DE DE19792941575 patent/DE2941575A1/de not_active Withdrawn
- 1979-10-15 GB GB7935776A patent/GB2034464B/en not_active Expired
- 1979-10-18 SE SE7908656A patent/SE7908656L/ not_active Application Discontinuation
- 1979-10-18 CA CA337,922A patent/CA1127536A/fr not_active Expired
- 1979-10-19 FR FR7926072A patent/FR2439233A1/fr active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| FR2439233B1 (fr) | 1985-03-08 |
| SE7908656L (sv) | 1980-04-20 |
| GB2034464B (en) | 1983-05-25 |
| FR2439233A1 (fr) | 1980-05-16 |
| GB2034464A (en) | 1980-06-04 |
| DE2941575A1 (de) | 1980-04-30 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MKEX | Expiry |