CA1128417A - Antigene nucleaire conjuge a la serum albumine methylee pouvant etre extrait - Google Patents

Antigene nucleaire conjuge a la serum albumine methylee pouvant etre extrait

Info

Publication number: CA1128417A
Authority: CA; Canada
Prior art keywords: dna; antibodies; deoxyribonucleic acid; serum albumin; flocculate
Prior art date: 1978-06-02
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired

Application number

CA388,887A

Other languages

English (en)

Inventor

Delfin F. Rippe

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

American Hospital Supply Corp

Original Assignee

American Hospital Supply Corp

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1978-06-02

Filing date

1981-10-27

Publication date

1982-07-27

1978-06-02 Priority claimed from US05/911,865 external-priority patent/US4234563A/en

1981-10-27 Application filed by American Hospital Supply Corp filed Critical American Hospital Supply Corp

1981-10-27 Priority to CA388,887A priority Critical patent/CA1128417A/fr

1982-07-27 Application granted granted Critical

1982-07-27 Publication of CA1128417A publication Critical patent/CA1128417A/fr

Status Expired legal-status Critical Current

Links

102000019040 Nuclear Antigens Human genes 0.000 title claims description 6
108010051791 Nuclear Antigens Proteins 0.000 title claims description 6
108010071390 Serum Albumin Proteins 0.000 title claims description 6
102000007562 Serum Albumin Human genes 0.000 title claims description 6
108010077055 methylated bovine serum albumin Proteins 0.000 claims abstract description 35
238000003556 assay Methods 0.000 claims abstract description 19
238000001514 detection method Methods 0.000 claims abstract description 12
239000007790 solid phase Substances 0.000 claims description 9
108091006905 Human Serum Albumin Proteins 0.000 claims description 5
102000008100 Human Serum Albumin Human genes 0.000 claims description 5
239000003153 chemical reaction reagent Substances 0.000 claims 2
208000003250 Mixed connective tissue disease Diseases 0.000 claims 1
102000053602 DNA Human genes 0.000 abstract description 41
108020004414 DNA Proteins 0.000 abstract description 41
210000002966 serum Anatomy 0.000 abstract description 31
201000000596 systemic lupus erythematosus Diseases 0.000 abstract description 27
239000000203 mixture Substances 0.000 abstract description 12
239000006228 supernatant Substances 0.000 abstract description 9
239000012530 fluid Substances 0.000 abstract description 8
238000005406 washing Methods 0.000 abstract description 8
238000000034 method Methods 0.000 abstract description 7
GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 abstract description 6
239000002244 precipitate Substances 0.000 abstract description 6
239000008188 pellet Substances 0.000 abstract description 5
230000003460 anti-nuclear Effects 0.000 abstract description 4
239000000758 substrate Substances 0.000 abstract description 4
229960002175 thyroglobulin Drugs 0.000 abstract description 4
102000009843 Thyroglobulin Human genes 0.000 abstract description 3
108010034949 Thyroglobulin Proteins 0.000 abstract description 3
208000023275 Autoimmune disease Diseases 0.000 abstract description 2
108010033040 Histones Proteins 0.000 abstract 1
239000007788 liquid Substances 0.000 abstract 1
108090000623 proteins and genes Proteins 0.000 abstract 1
102000004169 proteins and genes Human genes 0.000 abstract 1
239000000562 conjugate Substances 0.000 description 19
229940027941 immunoglobulin g Drugs 0.000 description 15
LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 11
239000002953 phosphate buffered saline Substances 0.000 description 11
238000003127 radioimmunoassay Methods 0.000 description 9
229920000642 polymer Polymers 0.000 description 8
244000309466 calf Species 0.000 description 5
239000000523 sample Substances 0.000 description 5
210000001541 thymus gland Anatomy 0.000 description 5
241000283973 Oryctolagus cuniculus Species 0.000 description 4
238000005119 centrifugation Methods 0.000 description 4
239000000243 solution Substances 0.000 description 4
101000800133 Homo sapiens Thyroglobulin Proteins 0.000 description 3
239000002253 acid Substances 0.000 description 3
230000003172 anti-dna Effects 0.000 description 3
238000002474 experimental method Methods 0.000 description 3
102000047688 human TG Human genes 0.000 description 3
206010025135 lupus erythematosus Diseases 0.000 description 3
241000283707 Capra Species 0.000 description 2
SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 2
230000015572 biosynthetic process Effects 0.000 description 2
238000010790 dilution Methods 0.000 description 2
239000012895 dilution Substances 0.000 description 2
ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 2
229960005542 ethidium bromide Drugs 0.000 description 2
230000005284 excitation Effects 0.000 description 2
238000010166 immunofluorescence Methods 0.000 description 2
230000005764 inhibitory process Effects 0.000 description 2
238000002156 mixing Methods 0.000 description 2
230000010807 negative regulation of binding Effects 0.000 description 2
238000001556 precipitation Methods 0.000 description 2
239000002195 soluble material Substances 0.000 description 2
239000000725 suspension Substances 0.000 description 2
FBOUIAKEJMZPQG-AWNIVKPZSA-N (1E)-1-(2,4-dichlorophenyl)-4,4-dimethyl-2-(1,2,4-triazol-1-yl)pent-1-en-3-ol Chemical compound C1=NC=NN1/C(C(O)C(C)(C)C)=C/C1=CC=C(Cl)C=C1Cl FBOUIAKEJMZPQG-AWNIVKPZSA-N 0.000 description 1
-1 4 ml PBS was added Chemical compound 0.000 description 1
241000972773 Aulopiformes Species 0.000 description 1
241000283690 Bos taurus Species 0.000 description 1
108091003079 Bovine Serum Albumin Proteins 0.000 description 1
241000588724 Escherichia coli Species 0.000 description 1
208000030836 Hashimoto thyroiditis Diseases 0.000 description 1
241000473945 Theria <moth genus> Species 0.000 description 1
238000004458 analytical method Methods 0.000 description 1
239000000427 antigen Substances 0.000 description 1
102000036639 antigens Human genes 0.000 description 1
108091007433 antigens Proteins 0.000 description 1
229940098773 bovine serum albumin Drugs 0.000 description 1
238000006243 chemical reaction Methods 0.000 description 1
239000003795 chemical substances by application Substances 0.000 description 1
230000008878 coupling Effects 0.000 description 1
238000010168 coupling process Methods 0.000 description 1
238000005859 coupling reaction Methods 0.000 description 1
201000010099 disease Diseases 0.000 description 1
208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 1
239000007850 fluorescent dye Substances 0.000 description 1
238000007421 fluorometric assay Methods 0.000 description 1
239000011521 glass Substances 0.000 description 1
230000003053 immunization Effects 0.000 description 1
238000002649 immunization Methods 0.000 description 1
239000003112 inhibitor Substances 0.000 description 1
238000012092 latex agglutination test Methods 0.000 description 1
239000011824 nuclear material Substances 0.000 description 1
238000001821 nucleic acid purification Methods 0.000 description 1
102000039446 nucleic acids Human genes 0.000 description 1
108020004707 nucleic acids Proteins 0.000 description 1
150000007523 nucleic acids Chemical class 0.000 description 1
QYSGYZVSCZSLHT-UHFFFAOYSA-N octafluoropropane Chemical class FC(F)(F)C(F)(F)C(F)(F)F QYSGYZVSCZSLHT-UHFFFAOYSA-N 0.000 description 1
239000012071 phase Substances 0.000 description 1
239000008363 phosphate buffer Substances 0.000 description 1
230000009257 reactivity Effects 0.000 description 1
206010039073 rheumatoid arthritis Diseases 0.000 description 1
235000019515 salmon Nutrition 0.000 description 1
230000035945 sensitivity Effects 0.000 description 1
238000000527 sonication Methods 0.000 description 1
230000006641 stabilisation Effects 0.000 description 1
238000011105 stabilization Methods 0.000 description 1
230000009885 systemic effect Effects 0.000 description 1
210000001519 tissue Anatomy 0.000 description 1
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1

Landscapes

Investigating Or Analysing Biological Materials (AREA)

CA388,887A 1978-06-02 1981-10-27 Antigene nucleaire conjuge a la serum albumine methylee pouvant etre extrait Expired CA1128417A (fr)

Priority Applications (1)

Application Number	Priority Date	Filing Date	Title
CA388,887A CA1128417A (fr)	1978-06-02	1981-10-27	Antigene nucleaire conjuge a la serum albumine methylee pouvant etre extrait

Applications Claiming Priority (4)

Application Number	Priority Date	Filing Date	Title
US05/911,865 US4234563A (en)	1978-06-02	1978-06-02	Insolubilized deoxyribonucleic acid (DNA)
CA000328899A CA1117008A (fr)	1978-06-02	1979-06-01	Acide albumine-desoxyribonucleique serique insoluble utilise dans des essais indirects de fluorescence pour les anticorps de (n-adn)
CA388,887A CA1128417A (fr)	1978-06-02	1981-10-27	Antigene nucleaire conjuge a la serum albumine methylee pouvant etre extrait
US911,865		1992-07-10

Publications (1)

Publication Number	Publication Date
CA1128417A true CA1128417A (fr)	1982-07-27

Family

ID=27166271

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CA388,887A Expired CA1128417A (fr)	1978-06-02	1981-10-27	Antigene nucleaire conjuge a la serum albumine methylee pouvant etre extrait

Country Status (1)

Country	Link
CA (1)	CA1128417A (fr)

1981
- 1981-10-27 CA CA388,887A patent/CA1128417A/fr not_active Expired

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Legal Events

Date	Code	Title	Description
1999-07-27	MKEX	Expiry