CA1250231A - Element multicouche d'analyse - Google Patents

Element multicouche d'analyse

Info

Publication number: CA1250231A
Authority: CA; Canada
Prior art keywords: layer; reagent; analyte; detectable species; diffusible
Prior art date: 1984-06-04
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired

Application number

CA000455810A

Other languages

English (en)

Inventor

Carl M. Berke

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Polaroid Corp

Original Assignee

Polaroid Corp

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1984-06-04

Filing date

1984-06-04

Publication date

1989-02-21

1984-06-04 Application filed by Polaroid Corp filed Critical Polaroid Corp

1984-06-04 Priority to CA000455810A priority Critical patent/CA1250231A/fr

1989-02-21 Application granted granted Critical

1989-02-21 Publication of CA1250231A publication Critical patent/CA1250231A/fr

Status Expired legal-status Critical Current

Links

238000004458 analytical method Methods 0.000 title claims abstract description 15
239000003153 chemical reaction reagent Substances 0.000 claims abstract description 41
239000012491 analyte Substances 0.000 claims abstract description 34
238000006243 chemical reaction Methods 0.000 claims abstract description 34
239000007788 liquid Substances 0.000 claims abstract description 22
239000000427 antigen Substances 0.000 claims description 42
102000036639 antigens Human genes 0.000 claims description 41
108091007433 antigens Proteins 0.000 claims description 41
238000000034 method Methods 0.000 claims description 26
229920000936 Agarose Polymers 0.000 claims description 23
230000005855 radiation Effects 0.000 claims description 21
239000000463 material Substances 0.000 claims description 15
GWEVSGVZZGPLCZ-UHFFFAOYSA-N Titan oxide Chemical compound O=[Ti]=O GWEVSGVZZGPLCZ-UHFFFAOYSA-N 0.000 claims description 14
230000007480 spreading Effects 0.000 claims description 13
238000001514 detection method Methods 0.000 claims description 11
238000009792 diffusion process Methods 0.000 claims description 10
239000004408 titanium dioxide Substances 0.000 claims description 6
239000004793 Polystyrene Substances 0.000 claims description 5
239000000839 emulsion Substances 0.000 claims description 2
239000004744 fabric Substances 0.000 claims description 2
230000000717 retained effect Effects 0.000 claims description 2
239000012857 radioactive material Substances 0.000 claims 1
239000010410 layer Substances 0.000 description 122
241000894007 species Species 0.000 description 45
239000000523 sample Substances 0.000 description 17
239000000126 substance Substances 0.000 description 17
239000000243 solution Substances 0.000 description 14
238000012360 testing method Methods 0.000 description 14
239000000203 mixture Substances 0.000 description 8
229920000642 polymer Polymers 0.000 description 8
108060003951 Immunoglobulin Proteins 0.000 description 7
238000000576 coating method Methods 0.000 description 7
102000018358 immunoglobulin Human genes 0.000 description 7
238000001114 immunoprecipitation Methods 0.000 description 7
108010010803 Gelatin Proteins 0.000 description 6
239000000084 colloidal system Substances 0.000 description 6
239000008273 gelatin Substances 0.000 description 6
229920000159 gelatin Polymers 0.000 description 6
235000019322 gelatine Nutrition 0.000 description 6
235000011852 gelatine desserts Nutrition 0.000 description 6
239000000047 product Substances 0.000 description 6
239000000049 pigment Substances 0.000 description 5
229920001223 polyethylene glycol Polymers 0.000 description 5
239000010409 thin film Substances 0.000 description 5
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
BPYKTIZUTYGOLE-IFADSCNNSA-N Bilirubin Chemical compound N1C(=O)C(C)=C(C=C)\C1=C\C1=C(C)C(CCC(O)=O)=C(CC2=C(C(C)=C(\C=C/3C(=C(C=C)C(=O)N\3)C)N2)CCC(O)=O)N1 BPYKTIZUTYGOLE-IFADSCNNSA-N 0.000 description 4
239000011248 coating agent Substances 0.000 description 4
LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 4
230000002401 inhibitory effect Effects 0.000 description 4
239000002953 phosphate buffered saline Substances 0.000 description 4
229920002223 polystyrene Polymers 0.000 description 4
229920000036 polyvinylpyrrolidone Polymers 0.000 description 4
239000001267 polyvinylpyrrolidone Substances 0.000 description 4
235000013855 polyvinylpyrrolidone Nutrition 0.000 description 4
239000000376 reactant Substances 0.000 description 4
238000000926 separation method Methods 0.000 description 4
229920000663 Hydroxyethyl cellulose Polymers 0.000 description 3
239000004354 Hydroxyethyl cellulose Substances 0.000 description 3
239000002202 Polyethylene glycol Substances 0.000 description 3
229920002472 Starch Polymers 0.000 description 3
230000000903 blocking effect Effects 0.000 description 3
230000002860 competitive effect Effects 0.000 description 3
238000006073 displacement reaction Methods 0.000 description 3
239000012153 distilled water Substances 0.000 description 3
238000001914 filtration Methods 0.000 description 3
239000012530 fluid Substances 0.000 description 3
230000006870 function Effects 0.000 description 3
235000019447 hydroxyethyl cellulose Nutrition 0.000 description 3
239000003446 ligand Substances 0.000 description 3
238000005259 measurement Methods 0.000 description 3
230000005012 migration Effects 0.000 description 3
238000013508 migration Methods 0.000 description 3
230000035945 sensitivity Effects 0.000 description 3
239000008107 starch Substances 0.000 description 3
235000019698 starch Nutrition 0.000 description 3
MPNXSZJPSVBLHP-UHFFFAOYSA-N 2-chloro-n-phenylpyridine-3-carboxamide Chemical compound ClC1=NC=CC=C1C(=O)NC1=CC=CC=C1 MPNXSZJPSVBLHP-UHFFFAOYSA-N 0.000 description 2
102000009027 Albumins Human genes 0.000 description 2
108010088751 Albumins Proteins 0.000 description 2
241000283707 Capra Species 0.000 description 2
FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 2
102000008100 Human Serum Albumin Human genes 0.000 description 2
108091006905 Human Serum Albumin Proteins 0.000 description 2
229920003171 Poly (ethylene oxide) Polymers 0.000 description 2
102000007562 Serum Albumin Human genes 0.000 description 2
108010071390 Serum Albumin Proteins 0.000 description 2
239000000654 additive Substances 0.000 description 2
239000007795 chemical reaction product Substances 0.000 description 2
150000001875 compounds Chemical class 0.000 description 2
238000000354 decomposition reaction Methods 0.000 description 2
239000006185 dispersion Substances 0.000 description 2
230000000694 effects Effects 0.000 description 2
GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 2
239000000499 gel Substances 0.000 description 2
238000003018 immunoassay Methods 0.000 description 2
230000002452 interceptive effect Effects 0.000 description 2
239000011229 interlayer Substances 0.000 description 2
230000000670 limiting effect Effects 0.000 description 2
239000011159 matrix material Substances 0.000 description 2
230000007246 mechanism Effects 0.000 description 2
TWNQGVIAIRXVLR-UHFFFAOYSA-N oxo(oxoalumanyloxy)alumane Chemical compound O=[Al]O[Al]=O TWNQGVIAIRXVLR-UHFFFAOYSA-N 0.000 description 2
-1 p-nonylphenoxy Chemical group 0.000 description 2
239000002245 particle Substances 0.000 description 2
230000002028 premature Effects 0.000 description 2
230000008569 process Effects 0.000 description 2
239000000700 radioactive tracer Substances 0.000 description 2
239000002904 solvent Substances 0.000 description 2
239000000600 sorbitol Substances 0.000 description 2
238000004381 surface treatment Methods 0.000 description 2
239000004094 surface-active agent Substances 0.000 description 2
229920002554 vinyl polymer Polymers 0.000 description 2
239000011800 void material Substances 0.000 description 2
KFDVPJUYSDEJTH-UHFFFAOYSA-N 4-ethenylpyridine Chemical compound C=CC1=CC=NC=C1 KFDVPJUYSDEJTH-UHFFFAOYSA-N 0.000 description 1
241000331231 Amorphocerini gen. n. 1 DAD-2008 Species 0.000 description 1
NLZUEZXRPGMBCV-UHFFFAOYSA-N Butylhydroxytoluene Chemical compound CC1=CC(C(C)(C)C)=C(O)C(C(C)(C)C)=C1 NLZUEZXRPGMBCV-UHFFFAOYSA-N 0.000 description 1
239000004971 Cross linker Substances 0.000 description 1
FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 description 1
229920002307 Dextran Polymers 0.000 description 1
102000004190 Enzymes Human genes 0.000 description 1
108090000790 Enzymes Proteins 0.000 description 1
229920001917 Ficoll Polymers 0.000 description 1
CTKINSOISVBQLD-UHFFFAOYSA-N Glycidol Chemical compound OCC1CO1 CTKINSOISVBQLD-UHFFFAOYSA-N 0.000 description 1
229920000084 Gum arabic Polymers 0.000 description 1
239000000899 Gutta-Percha Substances 0.000 description 1
101100412856 Mus musculus Rhod gene Proteins 0.000 description 1
241001163743 Perlodes Species 0.000 description 1
239000004698 Polyethylene Substances 0.000 description 1
239000004372 Polyvinyl alcohol Substances 0.000 description 1
241000978776 Senegalia senegal Species 0.000 description 1
VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
ATJFFYVFTNAWJD-UHFFFAOYSA-N Tin Chemical compound [Sn] ATJFFYVFTNAWJD-UHFFFAOYSA-N 0.000 description 1
101710123661 Venom allergen 5 Proteins 0.000 description 1
VSYMNDBTCKIDLT-UHFFFAOYSA-N [2-(carbamoyloxymethyl)-2-ethylbutyl] carbamate Chemical compound NC(=O)OCC(CC)(CC)COC(N)=O VSYMNDBTCKIDLT-UHFFFAOYSA-N 0.000 description 1
238000010521 absorption reaction Methods 0.000 description 1
239000000205 acacia gum Substances 0.000 description 1
235000010489 acacia gum Nutrition 0.000 description 1
239000002253 acid Substances 0.000 description 1
239000000853 adhesive Substances 0.000 description 1
230000001070 adhesive effect Effects 0.000 description 1
239000002998 adhesive polymer Substances 0.000 description 1
238000007605 air drying Methods 0.000 description 1
239000010425 asbestos Substances 0.000 description 1
230000003935 attention Effects 0.000 description 1
230000004888 barrier function Effects 0.000 description 1
239000011230 binding agent Substances 0.000 description 1
230000005540 biological transmission Effects 0.000 description 1
238000004364 calculation method Methods 0.000 description 1
239000001913 cellulose Substances 0.000 description 1
229920002678 cellulose Polymers 0.000 description 1
229920002301 cellulose acetate Polymers 0.000 description 1
230000008859 change Effects 0.000 description 1
239000008119 colloidal silica Substances 0.000 description 1
230000000052 comparative effect Effects 0.000 description 1
238000010668 complexation reaction Methods 0.000 description 1
239000012141 concentrate Substances 0.000 description 1
239000013068 control sample Substances 0.000 description 1
230000009849 deactivation Effects 0.000 description 1
238000010790 dilution Methods 0.000 description 1
239000012895 dilution Substances 0.000 description 1
230000003292 diminished effect Effects 0.000 description 1
DTVMXQFQTCJMOR-UHFFFAOYSA-N dioctyl propanedioate Chemical compound CCCCCCCCOC(=O)CC(=O)OCCCCCCCC DTVMXQFQTCJMOR-UHFFFAOYSA-N 0.000 description 1
NJLLQSBAHIKGKF-UHFFFAOYSA-N dipotassium dioxido(oxo)titanium Chemical compound [K+].[K+].[O-][Ti]([O-])=O NJLLQSBAHIKGKF-UHFFFAOYSA-N 0.000 description 1
NJDNXYGOVLYJHP-UHFFFAOYSA-L disodium;2-(3-oxido-6-oxoxanthen-9-yl)benzoate Chemical compound [Na+].[Na+].[O-]C(=O)C1=CC=CC=C1C1=C2C=CC(=O)C=C2OC2=CC([O-])=CC=C21 NJDNXYGOVLYJHP-UHFFFAOYSA-L 0.000 description 1
238000010494 dissociation reaction Methods 0.000 description 1
230000005593 dissociations Effects 0.000 description 1
238000001704 evaporation Methods 0.000 description 1
230000008020 evaporation Effects 0.000 description 1
230000005284 excitation Effects 0.000 description 1
238000001125 extrusion Methods 0.000 description 1
239000002657 fibrous material Substances 0.000 description 1
239000010408 film Substances 0.000 description 1
239000012847 fine chemical Substances 0.000 description 1
MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 1
239000011521 glass Substances 0.000 description 1
150000004676 glycans Chemical class 0.000 description 1
229910052739 hydrogen Inorganic materials 0.000 description 1
239000001257 hydrogen Substances 0.000 description 1
238000005213 imbibition Methods 0.000 description 1
230000003100 immobilizing effect Effects 0.000 description 1
229940027941 immunoglobulin g Drugs 0.000 description 1
229940072221 immunoglobulins Drugs 0.000 description 1
239000012784 inorganic fiber Substances 0.000 description 1
150000002500 ions Chemical class 0.000 description 1
238000003367 kinetic assay Methods 0.000 description 1
238000002372 labelling Methods 0.000 description 1
238000000464 low-speed centrifugation Methods 0.000 description 1
238000004519 manufacturing process Methods 0.000 description 1
230000001404 mediated effect Effects 0.000 description 1
239000012528 membrane Substances 0.000 description 1
239000002736 nonionic surfactant Substances 0.000 description 1
239000000123 paper Substances 0.000 description 1
239000011236 particulate material Substances 0.000 description 1
230000035699 permeability Effects 0.000 description 1
229920002401 polyacrylamide Polymers 0.000 description 1
229920000515 polycarbonate Polymers 0.000 description 1
239000004417 polycarbonate Substances 0.000 description 1
229920000573 polyethylene Polymers 0.000 description 1
229920002557 polyglycidol polymer Polymers 0.000 description 1
229920001282 polysaccharide Polymers 0.000 description 1
239000005017 polysaccharide Substances 0.000 description 1
229920002451 polyvinyl alcohol Polymers 0.000 description 1
239000011148 porous material Substances 0.000 description 1
239000002244 precipitate Substances 0.000 description 1
238000001556 precipitation Methods 0.000 description 1
102000004169 proteins and genes Human genes 0.000 description 1
108090000623 proteins and genes Proteins 0.000 description 1
230000002285 radioactive effect Effects 0.000 description 1
230000035484 reaction time Effects 0.000 description 1
238000002310 reflectometry Methods 0.000 description 1
238000009877 rendering Methods 0.000 description 1
230000003307 reticuloendothelial effect Effects 0.000 description 1
229910052895 riebeckite Inorganic materials 0.000 description 1
238000001179 sorption measurement Methods 0.000 description 1
230000000007 visual effect Effects 0.000 description 1
239000002982 water resistant material Substances 0.000 description 1

Landscapes

Investigating Or Analysing Biological Materials (AREA)

CA000455810A 1984-06-04 1984-06-04 Element multicouche d'analyse Expired CA1250231A (fr)

Priority Applications (1)

Application Number	Priority Date	Filing Date	Title
CA000455810A CA1250231A (fr)	1984-06-04	1984-06-04	Element multicouche d'analyse

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
CA000455810A CA1250231A (fr)	1984-06-04	1984-06-04	Element multicouche d'analyse

Publications (1)

Publication Number	Publication Date
CA1250231A true CA1250231A (fr)	1989-02-21

Family

ID=4128027

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CA000455810A Expired CA1250231A (fr)	1984-06-04	1984-06-04	Element multicouche d'analyse

Country Status (1)

Country	Link
CA (1)	CA1250231A (fr)

1984
- 1984-06-04 CA CA000455810A patent/CA1250231A/fr not_active Expired

Legal Events

Date	Code	Title	Description
2006-02-21	MKEX	Expiry

Publication	Publication Date	Title
US4459358A (en)	1984-07-10	Multilayer element for analysis
CA1267081A (fr)	1990-03-27	Element analytique multizone et reactif marque
US4806312A (en)	1989-02-21	Multizone analytical element having detectable signal concentrating zone
EP0070300B1 (fr)	1987-11-19	Systeme en phase solide pour essai avec agent de liaison
CA2375453C (fr)	2009-04-28	Papier reactif en ecoulement lateral bidirectionnel et procede
AU619480B2 (en)	1992-01-30	Lateral flow chromatographic binding assay device
US5212060A (en)	1993-05-18	Dry test strip comprising a dextran barrier for excluding erythrocytes
US4870005A (en)	1989-09-26	Multilayer analysis element
US4144306A (en)	1979-03-13	Element for analysis of liquids
CA2091255C (fr)	2001-11-06	Micro-analyse sur carte
US5217905A (en)	1993-06-08	Device and method for the rapid qualitative and quantitative determination of the presence of a reactive ligand in a fluid
US4956302A (en)	1990-09-11	Lateral flow chromatographic binding assay device
EP0198011B2 (fr)	1998-12-16	Essai d'identification de ligands sur le terrain
EP0726462A1 (fr)	1996-08-14	Appareil et méthode pour un essai de liaison sur un matériau porteur absorbant
JPH0278934A (ja)	1990-03-19	液体試料の成分を分析測定するためのテスト担体
KR20060126964A (ko)	2006-12-11	분석 장치의 동적 검출 범위의 확장 방법
US5958339A (en)	1999-09-28	Format for immunoassay in thin film
JPH0648271B2 (ja)	1994-06-22	液体試料の成分を測定するためのテストキヤリヤおよびその方法
US20100317126A1 (en)	2010-12-16	Agglutination assay method in porous medium layer
CA1250231A (fr)	1989-02-21	Element multicouche d'analyse
AU576771B2 (en)	1988-09-08	Multilayer element for analysis
US20050153460A1 (en)	2005-07-14	Agglutination assay method in binder medium
JPH0424660B2 (fr)	1992-04-27
USH1664H (en)	1997-07-01	Analytical element for immunoassay and method for its preparation
JPH01237455A (ja)	1989-09-21	乾式免疫分析要素