CA1266435A - Facteur partiellement purifie favorisant la croissance du tissu osseux - Google Patents

Facteur partiellement purifie favorisant la croissance du tissu osseux

Info

Publication number: CA1266435A
Authority: CA; Canada
Prior art keywords: bone; partially purified; factor; inducing factor; cmc
Prior art date: 1986-04-24
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired

Application number

CA000507485A

Other languages

English (en)

Inventor

Saeid Seyedin

Thomas Thomas

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Celtrix Pharmaceuticals Inc

Original Assignee

Collagen Corp

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1986-04-24

Filing date

1986-04-24

Publication date

1990-03-06

1986-04-24 Application filed by Collagen Corp filed Critical Collagen Corp

1986-04-24 Priority to CA000507485A priority Critical patent/CA1266435A/fr

1990-03-06 Application granted granted Critical

1990-03-06 Publication of CA1266435A publication Critical patent/CA1266435A/fr

Status Expired legal-status Critical Current

Links

210000000988 bone and bone Anatomy 0.000 title claims abstract description 49
230000001939 inductive effect Effects 0.000 title claims abstract description 18
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 22
229920002134 Carboxymethyl cellulose Polymers 0.000 claims abstract description 15
239000001768 carboxy methyl cellulose Substances 0.000 claims abstract description 11
235000010948 carboxy methyl cellulose Nutrition 0.000 claims abstract description 11
239000008112 carboxymethyl-cellulose Substances 0.000 claims abstract description 11
239000011780 sodium chloride Substances 0.000 claims abstract description 11
230000003196 chaotropic effect Effects 0.000 claims abstract description 10
239000003795 chemical substances by application Substances 0.000 claims abstract description 8
238000002523 gelfiltration Methods 0.000 claims abstract description 6
102000004169 proteins and genes Human genes 0.000 claims description 31
108090000623 proteins and genes Proteins 0.000 claims description 31
238000000034 method Methods 0.000 claims description 21
239000000463 material Substances 0.000 claims description 13
PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical group [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 claims description 9
239000007943 implant Substances 0.000 claims description 9
150000001768 cations Chemical class 0.000 claims description 6
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229960000789 guanidine hydrochloride Drugs 0.000 claims description 5
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RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 5
HDFGOPSGAURCEO-UHFFFAOYSA-N N-ethylmaleimide Chemical compound CCN1C(=O)C=CC1=O HDFGOPSGAURCEO-UHFFFAOYSA-N 0.000 description 5
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GHAZCVNUKKZTLG-UHFFFAOYSA-N N-ethyl-succinimide Natural products CCN1C(=O)CCC1=O GHAZCVNUKKZTLG-UHFFFAOYSA-N 0.000 description 4
PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 4
XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 4
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XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 3
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239000008367 deionised water Substances 0.000 description 3
229910021641 deionized water Inorganic materials 0.000 description 3
LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 3
239000012091 fetal bovine serum Substances 0.000 description 3
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LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
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DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 2
241000124008 Mammalia Species 0.000 description 2
101000986989 Naja kaouthia Acidic phospholipase A2 CM-II Proteins 0.000 description 2
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239000012506 Sephacryl® Substances 0.000 description 2
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239000011543 agarose gel Substances 0.000 description 2
150000001413 amino acids Chemical class 0.000 description 2
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238000002360 preparation method Methods 0.000 description 2
238000004007 reversed phase HPLC Methods 0.000 description 2
229910052708 sodium Inorganic materials 0.000 description 2
UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 description 1
GEYOCULIXLDCMW-UHFFFAOYSA-N 1,2-phenylenediamine Chemical compound NC1=CC=CC=C1N GEYOCULIXLDCMW-UHFFFAOYSA-N 0.000 description 1
WCVOGSZTONGSQY-UHFFFAOYSA-N 2,4,6-trichloroanisole Chemical compound COC1=C(Cl)C=C(Cl)C=C1Cl WCVOGSZTONGSQY-UHFFFAOYSA-N 0.000 description 1
SLXKOJJOQWFEFD-UHFFFAOYSA-N 6-aminohexanoic acid Chemical compound NCCCCCC(O)=O SLXKOJJOQWFEFD-UHFFFAOYSA-N 0.000 description 1
108010088751 Albumins Proteins 0.000 description 1
102000009027 Albumins Human genes 0.000 description 1
CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 1
241000282693 Cercopithecidae Species 0.000 description 1
241000861718 Chloris <Aves> Species 0.000 description 1
235000019750 Crude protein Nutrition 0.000 description 1
229920002307 Dextran Polymers 0.000 description 1
241001465754 Metazoa Species 0.000 description 1
102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 description 1
108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 description 1
229920001213 Polysorbate 20 Polymers 0.000 description 1
239000004793 Polystyrene Substances 0.000 description 1
241000208474 Protea Species 0.000 description 1
241000700157 Rattus norvegicus Species 0.000 description 1
VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
239000004480 active ingredient Substances 0.000 description 1
238000007792 addition Methods 0.000 description 1
230000002411 adverse Effects 0.000 description 1
125000003275 alpha amino acid group Chemical group 0.000 description 1
125000000539 amino acid group Chemical group 0.000 description 1
229960002684 aminocaproic acid Drugs 0.000 description 1
238000004458 analytical method Methods 0.000 description 1
238000005349 anion exchange Methods 0.000 description 1
150000001450 anions Chemical class 0.000 description 1
239000003242 anti bacterial agent Substances 0.000 description 1
229940088710 antibiotic agent Drugs 0.000 description 1
239000000427 antigen Substances 0.000 description 1
102000036639 antigens Human genes 0.000 description 1
108091007433 antigens Proteins 0.000 description 1
239000012298 atmosphere Substances 0.000 description 1
230000003190 augmentative effect Effects 0.000 description 1
PXXJHWLDUBFPOL-UHFFFAOYSA-N benzamidine Chemical compound NC(=N)C1=CC=CC=C1 PXXJHWLDUBFPOL-UHFFFAOYSA-N 0.000 description 1
238000004166 bioassay Methods 0.000 description 1
OHJMTUPIZMNBFR-UHFFFAOYSA-N biuret Chemical compound NC(=O)NC(N)=O OHJMTUPIZMNBFR-UHFFFAOYSA-N 0.000 description 1
230000008468 bone growth Effects 0.000 description 1
239000001506 calcium phosphate Substances 0.000 description 1
235000011010 calcium phosphates Nutrition 0.000 description 1
235000011089 carbon dioxide Nutrition 0.000 description 1
238000004113 cell culture Methods 0.000 description 1
230000001413 cellular effect Effects 0.000 description 1
238000005119 centrifugation Methods 0.000 description 1
238000004587 chromatography analysis Methods 0.000 description 1
239000012141 concentrate Substances 0.000 description 1
239000013068 control sample Substances 0.000 description 1
230000009260 cross reactivity Effects 0.000 description 1
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238000012217 deletion Methods 0.000 description 1
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210000004268 dentin Anatomy 0.000 description 1
238000010586 diagram Methods 0.000 description 1
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238000010790 dilution Methods 0.000 description 1
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210000003754 fetus Anatomy 0.000 description 1
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230000006698 induction Effects 0.000 description 1
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210000001872 metatarsal bone Anatomy 0.000 description 1
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230000002138 osteoinductive effect Effects 0.000 description 1
206010033675 panniculitis Diseases 0.000 description 1
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235000017281 sodium acetate Nutrition 0.000 description 1
229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
239000007787 solid Substances 0.000 description 1
239000002904 solvent Substances 0.000 description 1
238000001179 sorption measurement Methods 0.000 description 1
239000007858 starting material Substances 0.000 description 1
229960005322 streptomycin Drugs 0.000 description 1
210000004304 subcutaneous tissue Anatomy 0.000 description 1
239000000758 substrate Substances 0.000 description 1
239000012134 supernatant fraction Substances 0.000 description 1
238000010257 thawing Methods 0.000 description 1
210000000115 thoracic cavity Anatomy 0.000 description 1
210000001519 tissue Anatomy 0.000 description 1
238000004454 trace mineral analysis Methods 0.000 description 1
QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical class [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
210000001364 upper extremity Anatomy 0.000 description 1

Landscapes

Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Peptides Or Proteins (AREA)

CA000507485A 1986-04-24 1986-04-24 Facteur partiellement purifie favorisant la croissance du tissu osseux Expired CA1266435A (fr)

Priority Applications (1)

Application Number	Priority Date	Filing Date	Title
CA000507485A CA1266435A (fr)	1986-04-24	1986-04-24	Facteur partiellement purifie favorisant la croissance du tissu osseux

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
CA000507485A CA1266435A (fr)	1986-04-24	1986-04-24	Facteur partiellement purifie favorisant la croissance du tissu osseux

Publications (1)

Publication Number	Publication Date
CA1266435A true CA1266435A (fr)	1990-03-06

Family

ID=4132958

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CA000507485A Expired CA1266435A (fr)	1986-04-24	1986-04-24	Facteur partiellement purifie favorisant la croissance du tissu osseux

Country Status (1)

Country	Link
CA (1)	CA1266435A (fr)

1986
- 1986-04-24 CA CA000507485A patent/CA1266435A/fr not_active Expired

Legal Events

Date	Code	Title	Description
2004-03-08	MKLA	Lapsed

Publication	Publication Date	Title
US4627982A (en)	1986-12-09	Partially purified bone-inducing factor
AU594949B2 (en)	1990-03-22	Partially purified bone-inducing factor
Sampath et al.	1983	Homology of bone-inductive proteins from human, monkey, bovine, and rat extracellular matrix.
US4843063A (en)	1989-06-27	Polypeptide cartilage-inducing factors found in bone
US5001169A (en)	1991-03-19	Inductive collagen-based bone repair preparations
US4563350A (en)	1986-01-07	Inductive collagen based bone repair preparations
CA1338663C (fr)	1996-10-22	Proteines d'osteogenese biosynthetiques et dispositifs d'osteogenese a base de ces proteines
Sampath et al.	1987	Isolation of osteogenin, an extracellular matrix-associated, bone-inductive protein, by heparin affinity chromatography.
US5108753A (en)	1992-04-28	Osteogenic devices
US5840325A (en)	1998-11-24	Osteogenic devices
Sampath et al.	1984	Distribution of bone inductive proteins in mineralized and demineralized extracellular matrix
EP1539812B1 (fr)	2010-06-02	Biomateriaux osteo-inducteurs
Sampath et al.	1984	Extracellular matrix proteins involved in bone induction are vitamin D dependent
CA1266435A (fr)	1990-03-06	Facteur partiellement purifie favorisant la croissance du tissu osseux
USRE35694E (en)	1997-12-16	Polypeptide cartilage-inducing factors found in bone
Yoshimura et al.	1993	Purification of water-soluble bone-inductive protein from bovine demineralized bone matrix
USRE34090E (en)	1992-10-06	Polypeptide cartilage-inducing factors found in bone
JPS62255429A (ja)	1987-11-07	部分精製骨誘導因子
ZA200500427B (en)	2006-04-26	Osteoinductive biomaterials