CA1292960C - Procede pour augmenter la teneur en lysine dans le pool libre du mais - Google Patents

Procede pour augmenter la teneur en lysine dans le pool libre du mais

Info

Publication number: CA1292960C
Authority: CA; Canada
Prior art keywords: medium; subculturing; aminoethyl; cysteine; concentration
Prior art date: 1987-11-30
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired - Fee Related

Application number

CA000553057A

Other languages

English (en)

Inventor

Judith P. Cook

Ernest T. Hubbard

Raghav Ram

Michele D. Hollingsworth

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Sungene Technologies Corp

Original Assignee

Sungene Technologies Corp

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1987-11-30

Filing date

1987-11-30

Publication date

1991-12-10

1987-11-30 Application filed by Sungene Technologies Corp filed Critical Sungene Technologies Corp

1987-11-30 Priority to CA000553057A priority Critical patent/CA1292960C/fr

1991-12-10 Application granted granted Critical

1991-12-10 Publication of CA1292960C publication Critical patent/CA1292960C/fr

2008-12-10 Anticipated expiration legal-status Critical

Status Expired - Fee Related legal-status Critical Current

Links

238000000034 method Methods 0.000 title claims abstract description 80
230000008569 process Effects 0.000 title claims abstract description 76
239000004472 Lysine Substances 0.000 title claims abstract description 61
KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 title claims abstract description 60
240000008042 Zea mays Species 0.000 title claims abstract description 55
235000002017 Zea mays subsp mays Nutrition 0.000 title claims abstract description 45
235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 title claims abstract description 30
235000009973 maize Nutrition 0.000 title claims abstract description 30
241000196324 Embryophyta Species 0.000 claims abstract description 88
206010020649 Hyperkeratosis Diseases 0.000 claims abstract description 80
GHSJKUNUIHUPDF-BYPYZUCNSA-N L-thialysine Chemical compound NCCSC[C@H](N)C(O)=O GHSJKUNUIHUPDF-BYPYZUCNSA-N 0.000 claims abstract description 27
230000008929 regeneration Effects 0.000 claims description 50
238000011069 regeneration method Methods 0.000 claims description 50
150000003839 salts Chemical class 0.000 claims description 33
229940088594 vitamin Drugs 0.000 claims description 29
229930003231 vitamin Natural products 0.000 claims description 29
235000013343 vitamin Nutrition 0.000 claims description 29
239000011782 vitamin Substances 0.000 claims description 29
230000006698 induction Effects 0.000 claims description 27
229930006000 Sucrose Natural products 0.000 claims description 23
CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 23
229910052500 inorganic mineral Inorganic materials 0.000 claims description 23
239000011707 mineral Substances 0.000 claims description 23
239000005720 sucrose Substances 0.000 claims description 23
238000012423 maintenance Methods 0.000 claims description 20
239000005556 hormone Substances 0.000 claims description 19
229940088597 hormone Drugs 0.000 claims description 19
UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 claims description 15
239000004062 cytokinin Substances 0.000 claims description 15
210000001161 mammalian embryo Anatomy 0.000 claims description 12
238000012258 culturing Methods 0.000 claims description 9
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239000004201 L-cysteine Substances 0.000 claims 1
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239000002609 medium Substances 0.000 description 251
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XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 35
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DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 21
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KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 20
235000010755 mineral Nutrition 0.000 description 19
SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 18
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239000008272 agar Substances 0.000 description 11
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NWBJYWHLCVSVIJ-UHFFFAOYSA-N N-benzyladenine Chemical compound N=1C=NC=2NC=NC=2C=1NCC1=CC=CC=C1 NWBJYWHLCVSVIJ-UHFFFAOYSA-N 0.000 description 10
239000002826 coolant Substances 0.000 description 10
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239000000243 solution Substances 0.000 description 10
239000003617 indole-3-acetic acid Substances 0.000 description 8
ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 7
101150114843 Mgll gene Proteins 0.000 description 7
AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 description 7
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239000011664 nicotinic acid Substances 0.000 description 7
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102000004169 proteins and genes Human genes 0.000 description 7
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239000002151 riboflavin Substances 0.000 description 7
239000002689 soil Substances 0.000 description 7
229940024606 amino acid Drugs 0.000 description 6
229960004106 citric acid Drugs 0.000 description 6
229960000367 inositol Drugs 0.000 description 6
CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 description 6
NLKNQRATVPKPDG-UHFFFAOYSA-M potassium iodide Chemical compound [K+].[I-] NLKNQRATVPKPDG-UHFFFAOYSA-M 0.000 description 6
FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 6
LXNHXLLTXMVWPM-UHFFFAOYSA-N pyridoxine Chemical compound CC1=NC=C(CO)C(CO)=C1O LXNHXLLTXMVWPM-UHFFFAOYSA-N 0.000 description 6
CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 description 6
QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 5
QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 5
238000004519 manufacturing process Methods 0.000 description 5
LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 5
DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 5
229960000344 thiamine hydrochloride Drugs 0.000 description 5
235000019190 thiamine hydrochloride Nutrition 0.000 description 5
239000011747 thiamine hydrochloride Substances 0.000 description 5
UZKQTCBAMSWPJD-UQCOIBPSSA-N trans-Zeatin Natural products OCC(/C)=C\CNC1=NC=NC2=C1N=CN2 UZKQTCBAMSWPJD-UQCOIBPSSA-N 0.000 description 5
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235000001014 amino acid Nutrition 0.000 description 4
150000001413 amino acids Chemical class 0.000 description 4
KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 4
239000004327 boric acid Substances 0.000 description 4
ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 4
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235000019157 thiamine Nutrition 0.000 description 4
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239000011721 thiamine Substances 0.000 description 4
KYMBYSLLVAOCFI-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SCN1CC1=CN=C(C)N=C1N KYMBYSLLVAOCFI-UHFFFAOYSA-N 0.000 description 4
GHOKWGTUZJEAQD-ZETCQYMHSA-N (D)-(+)-Pantothenic acid Chemical compound OCC(C)(C)[C@@H](O)C(=O)NCCC(O)=O GHOKWGTUZJEAQD-ZETCQYMHSA-N 0.000 description 3
WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 3
229930192334 Auxin Natural products 0.000 description 3
229930182816 L-glutamine Natural products 0.000 description 3
OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 3
230000009418 agronomic effect Effects 0.000 description 3
238000003556 assay Methods 0.000 description 3
239000002363 auxin Substances 0.000 description 3
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238000006243 chemical reaction Methods 0.000 description 3
IXORZMNAPKEEDV-OBDJNFEBSA-N gibberellin A3 Chemical compound C([C@@]1(O)C(=C)C[C@@]2(C1)[C@H]1C(O)=O)C[C@H]2[C@]2(C=C[C@@H]3O)[C@H]1[C@]3(C)C(=O)O2 IXORZMNAPKEEDV-OBDJNFEBSA-N 0.000 description 3
229910000402 monopotassium phosphate Inorganic materials 0.000 description 3
235000019796 monopotassium phosphate Nutrition 0.000 description 3
GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 3
239000004323 potassium nitrate Substances 0.000 description 3
235000010333 potassium nitrate Nutrition 0.000 description 3
238000004382 potting Methods 0.000 description 3
235000008160 pyridoxine Nutrition 0.000 description 3
239000011677 pyridoxine Substances 0.000 description 3
230000002829 reductive effect Effects 0.000 description 3
YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 3
229940011671 vitamin b6 Drugs 0.000 description 3
SODPIMGUZLOIPE-UHFFFAOYSA-N (4-chlorophenoxy)acetic acid Chemical compound OC(=O)COC1=CC=C(Cl)C=C1 SODPIMGUZLOIPE-UHFFFAOYSA-N 0.000 description 2
VDRACTHWBNITQP-UHFFFAOYSA-N 2-(3-methylbutyl)-7h-purin-6-amine Chemical compound CC(C)CCC1=NC(N)=C2NC=NC2=N1 VDRACTHWBNITQP-UHFFFAOYSA-N 0.000 description 2
RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical class [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 2
239000005980 Gibberellic acid Substances 0.000 description 2
VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 2
CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
RWRDLPDLKQPQOW-UHFFFAOYSA-N Pyrrolidine Chemical compound C1CCNC1 RWRDLPDLKQPQOW-UHFFFAOYSA-N 0.000 description 2
239000002253 acid Substances 0.000 description 2
230000002411 adverse Effects 0.000 description 2
BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 2
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235000011130 ammonium sulphate Nutrition 0.000 description 2
238000004458 analytical method Methods 0.000 description 2
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FAPWYRCQGJNNSJ-UBKPKTQASA-L calcium D-pantothenic acid Chemical compound [Ca+2].OCC(C)(C)[C@@H](O)C(=O)NCCC([O-])=O.OCC(C)(C)[C@@H](O)C(=O)NCCC([O-])=O FAPWYRCQGJNNSJ-UBKPKTQASA-L 0.000 description 2
LLSDKQJKOVVTOJ-UHFFFAOYSA-L calcium chloride dihydrate Chemical compound O.O.[Cl-].[Cl-].[Ca+2] LLSDKQJKOVVTOJ-UHFFFAOYSA-L 0.000 description 2
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239000010941 cobalt Chemical class 0.000 description 2
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GUTLYIVDDKVIGB-UHFFFAOYSA-N cobalt atom Chemical class [Co] GUTLYIVDDKVIGB-UHFFFAOYSA-N 0.000 description 2
150000001875 compounds Chemical class 0.000 description 2
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239000010949 copper Substances 0.000 description 2
238000001514 detection method Methods 0.000 description 2
238000011161 development Methods 0.000 description 2
239000012737 fresh medium Substances 0.000 description 2
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IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 description 2
238000000338 in vitro Methods 0.000 description 2
WRUGWIBCXHJTDG-UHFFFAOYSA-L magnesium sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Mg+2].[O-]S([O-])(=O)=O WRUGWIBCXHJTDG-UHFFFAOYSA-L 0.000 description 2
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ISPYRSDWRDQNSW-UHFFFAOYSA-L manganese(II) sulfate monohydrate Chemical compound O.[Mn+2].[O-]S([O-])(=O)=O ISPYRSDWRDQNSW-UHFFFAOYSA-L 0.000 description 2
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238000002955 isolation Methods 0.000 description 1
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239000011572 manganese Substances 0.000 description 1
239000012528 membrane Substances 0.000 description 1
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239000004570 mortar (masonry) Substances 0.000 description 1
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125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
229940042943 pyridoxine 2 mg Drugs 0.000 description 1
ZUFQODAHGAHPFQ-UHFFFAOYSA-N pyridoxine hydrochloride Chemical compound Cl.CC1=NC=C(CO)C(CO)=C1O ZUFQODAHGAHPFQ-UHFFFAOYSA-N 0.000 description 1
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229940001585 sodium molybdate(vi) Drugs 0.000 description 1
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241000894007 species Species 0.000 description 1
239000008223 sterile water Substances 0.000 description 1
238000006467 substitution reaction Methods 0.000 description 1
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235000019155 vitamin A Nutrition 0.000 description 1
239000011719 vitamin A Substances 0.000 description 1
230000004580 weight loss Effects 0.000 description 1
NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 1
229960001763 zinc sulfate Drugs 0.000 description 1
229910000368 zinc sulfate Inorganic materials 0.000 description 1
RZLVQBNCHSJZPX-UHFFFAOYSA-L zinc sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Zn+2].[O-]S([O-])(=O)=O RZLVQBNCHSJZPX-UHFFFAOYSA-L 0.000 description 1

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Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

CA000553057A 1987-11-30 1987-11-30 Procede pour augmenter la teneur en lysine dans le pool libre du mais Expired - Fee Related CA1292960C (fr)

Priority Applications (1)

Application Number	Priority Date	Filing Date	Title
CA000553057A CA1292960C (fr)	1987-11-30	1987-11-30	Procede pour augmenter la teneur en lysine dans le pool libre du mais

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
CA000553057A CA1292960C (fr)	1987-11-30	1987-11-30	Procede pour augmenter la teneur en lysine dans le pool libre du mais

Publications (1)

Publication Number	Publication Date
CA1292960C true CA1292960C (fr)	1991-12-10

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Family Applications (1)

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CA000553057A Expired - Fee Related CA1292960C (fr)	1987-11-30	1987-11-30	Procede pour augmenter la teneur en lysine dans le pool libre du mais

Country Status (1)

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CA (1)	CA1292960C (fr)

1987
- 1987-11-30 CA CA000553057A patent/CA1292960C/fr not_active Expired - Fee Related

Legal Events

Date	Code	Title	Description
1995-06-11	MKLA	Lapsed

Publication	Publication Date	Title
EP0271408A2 (fr)	1988-06-15	Procédé pour augmenter le contenu du pool de lysine libre dans le mais
Emershad et al.	1984	In‐ovulo embryo culture of Vitis vinifera LCV ‘Thompson seedless’
US4666844A (en)	1987-05-19	Process for regenerating cereals
US5445961A (en)	1995-08-29	Isolated microscope and anther culture of maize
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CA1269537A (fr)	1990-05-29	Procede pour la regeneration du mais
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US4684612A (en)	1987-08-04	Process for regenerating soybeans
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CA1292960C (fr)	1991-12-10	Procede pour augmenter la teneur en lysine dans le pool libre du mais
US5066595A (en)	1991-11-19	Process for increasing free pool lysine content in maize
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