CA1297406C - Methode pour la detection et la quantification des ligands dans des liquides - Google Patents
Methode pour la detection et la quantification des ligands dans des liquidesInfo
- Publication number
- CA1297406C CA1297406C CA000556618A CA556618A CA1297406C CA 1297406 C CA1297406 C CA 1297406C CA 000556618 A CA000556618 A CA 000556618A CA 556618 A CA556618 A CA 556618A CA 1297406 C CA1297406 C CA 1297406C
- Authority
- CA
- Canada
- Prior art keywords
- biotin
- ligand
- interest
- avidin
- labeled
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
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- 238000000034 method Methods 0.000 title claims abstract description 100
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- 229960002685 biotin Drugs 0.000 claims abstract description 187
- 239000011616 biotin Substances 0.000 claims abstract description 187
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- YMXHPSHLTSZXKH-RVBZMBCESA-N (2,5-dioxopyrrolidin-1-yl) 5-[(3as,4s,6ar)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoate Chemical compound C([C@H]1[C@H]2NC(=O)N[C@H]2CS1)CCCC(=O)ON1C(=O)CCC1=O YMXHPSHLTSZXKH-RVBZMBCESA-N 0.000 description 5
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Cell Biology (AREA)
- Pathology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Applications Claiming Priority (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US360487A | 1987-01-15 | 1987-01-15 | |
| US356687A | 1987-01-15 | 1987-01-15 | |
| US003,604 | 1987-01-15 | ||
| US003,566 | 1987-01-15 | ||
| US07/013,581 US4863876A (en) | 1987-01-15 | 1987-02-11 | Method of detecting and quantifying ligands in liquids via biotin-avidin-medicated fluorescence polarization |
| US013,581 | 1987-02-11 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1297406C true CA1297406C (fr) | 1992-03-17 |
Family
ID=27357435
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA000556618A Expired - Lifetime CA1297406C (fr) | 1987-01-15 | 1988-01-15 | Methode pour la detection et la quantification des ligands dans des liquides |
Country Status (4)
| Country | Link |
|---|---|
| JP (1) | JPH02502122A (fr) |
| AU (1) | AU1342488A (fr) |
| CA (1) | CA1297406C (fr) |
| WO (1) | WO1988005538A1 (fr) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IE64286B1 (en) * | 1988-05-25 | 1995-07-26 | Boehringer Mannheim Gmbh | Process for the determination of an immunologically detectable substance and a suitable vessel therefor |
| DE3834766A1 (de) * | 1988-10-12 | 1990-04-26 | Boehringer Mannheim Gmbh | Verfahren zur bestimmung einer spezifisch bindefaehigen substanz |
| GB8902689D0 (en) * | 1989-02-07 | 1989-03-30 | Ici Plc | Assay method |
| US6087188A (en) * | 1992-11-13 | 2000-07-11 | Alk A/S | Two-site immunoassay for an antibody with chemiluminescent label and biotin bound ligand |
| US6503709B1 (en) | 1997-07-03 | 2003-01-07 | Id Biomedical Corporation | Methods for rapidly detecting methicillin resistant staphylococci |
| DE60238928D1 (de) * | 2001-09-19 | 2011-02-24 | Masaaki Kai | Lumineszierendes polymer und seine verwendung im bioassay |
| CN107533052B (zh) * | 2015-05-11 | 2023-04-28 | 万迈医疗仪器有限公司 | 在免疫测定中重复使用测试探针和试剂的方法 |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0201211A1 (fr) * | 1985-04-10 | 1986-11-12 | Whittaker Corporation | Méthode et compositions pour immuno-essais visuels à phase solide basés sur des particules microsphériques et luminescente |
-
1988
- 1988-01-14 JP JP63501878A patent/JPH02502122A/ja active Pending
- 1988-01-14 AU AU13424/88A patent/AU1342488A/en not_active Abandoned
- 1988-01-14 WO PCT/US1988/000067 patent/WO1988005538A1/fr not_active Ceased
- 1988-01-15 CA CA000556618A patent/CA1297406C/fr not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JPH02502122A (ja) | 1990-07-12 |
| WO1988005538A1 (fr) | 1988-07-28 |
| AU1342488A (en) | 1988-08-10 |
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