CA1341365C - Composition a base de sequences de polynucleotides visant a detecter un analyte - Google Patents

Composition a base de sequences de polynucleotides visant a detecter un analyte Download PDF

Info

Publication number: CA1341365C
Authority: CA; Canada
Prior art keywords: composition according; nucleic acid; polynucleotide; entity; oligo
Prior art date: 1983-05-05
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired - Lifetime

Application number

CA000546847A

Other languages

English (en)

Inventor

Robert G. Pergolizzi

Jannis G. Stavrianopoulos

Elazar Rabbani

Dean L. Engelhardt

Stan Kline

Paula J. Olsiewski

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Enzo Biochem Inc

Original Assignee

Enzo Biochem Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1983-05-05

Filing date

1984-03-30

Publication date

2002-06-04

1984-03-30 Application filed by Enzo Biochem Inc filed Critical Enzo Biochem Inc

1984-03-30 Priority claimed from CA000451043A external-priority patent/CA1228811A/fr

2002-06-04 Application granted granted Critical

2002-06-04 Publication of CA1341365C publication Critical patent/CA1341365C/fr

2019-06-04 Anticipated expiration legal-status Critical

Status Expired - Lifetime legal-status Critical Current

Links

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NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
239000010452 phosphate Substances 0.000 description 1
150000003013 phosphoric acid derivatives Chemical class 0.000 description 1
229910052698 phosphorus Inorganic materials 0.000 description 1
239000013612 plasmid Substances 0.000 description 1
239000004033 plastic Substances 0.000 description 1
229920003023 plastic Polymers 0.000 description 1
229920002401 polyacrylamide Polymers 0.000 description 1
229920001282 polysaccharide Polymers 0.000 description 1
239000005017 polysaccharide Substances 0.000 description 1
235000015497 potassium bicarbonate Nutrition 0.000 description 1
229910000028 potassium bicarbonate Inorganic materials 0.000 description 1
239000011736 potassium bicarbonate Substances 0.000 description 1
CHKVPAROMQMJNQ-UHFFFAOYSA-M potassium bisulfate Chemical compound [K+].OS([O-])(=O)=O CHKVPAROMQMJNQ-UHFFFAOYSA-M 0.000 description 1
229910000343 potassium bisulfate Inorganic materials 0.000 description 1
TYJJADVDDVDEDZ-UHFFFAOYSA-M potassium hydrogencarbonate Chemical compound [K+].OC([O-])=O TYJJADVDDVDEDZ-UHFFFAOYSA-M 0.000 description 1
125000000075 primary alcohol group Chemical group 0.000 description 1
230000000644 propagated effect Effects 0.000 description 1
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239000002212 purine nucleoside Substances 0.000 description 1
150000003212 purines Chemical class 0.000 description 1
210000004915 pus Anatomy 0.000 description 1
229940107700 pyruvic acid Drugs 0.000 description 1
238000010791 quenching Methods 0.000 description 1
238000003127 radioimmunoassay Methods 0.000 description 1
230000009467 reduction Effects 0.000 description 1
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238000010992 reflux Methods 0.000 description 1
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238000010839 reverse transcription Methods 0.000 description 1
238000004007 reversed phase HPLC Methods 0.000 description 1
230000002441 reversible effect Effects 0.000 description 1
239000002336 ribonucleotide Substances 0.000 description 1
125000002652 ribonucleotide group Chemical group 0.000 description 1
238000002390 rotary evaporation Methods 0.000 description 1
210000003296 saliva Anatomy 0.000 description 1
239000011833 salt mixture Substances 0.000 description 1
229920006395 saturated elastomer Polymers 0.000 description 1
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238000000926 separation method Methods 0.000 description 1
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239000011734 sodium Substances 0.000 description 1
229910052708 sodium Inorganic materials 0.000 description 1
229910000029 sodium carbonate Inorganic materials 0.000 description 1
239000007787 solid Substances 0.000 description 1
239000011343 solid material Substances 0.000 description 1
239000002904 solvent Substances 0.000 description 1
229940063675 spermine Drugs 0.000 description 1
239000011550 stock solution Substances 0.000 description 1
238000003860 storage Methods 0.000 description 1
150000008163 sugars Chemical class 0.000 description 1
125000000446 sulfanediyl group Chemical group *S* 0.000 description 1
BDHFUVZGWQCTTF-UHFFFAOYSA-M sulfonate Chemical compound [O-]S(=O)=O BDHFUVZGWQCTTF-UHFFFAOYSA-M 0.000 description 1
230000002194 synthesizing effect Effects 0.000 description 1
125000003396 thiol group Chemical group [H]S* 0.000 description 1
229940104230 thymidine Drugs 0.000 description 1
238000007070 tosylation reaction Methods 0.000 description 1
238000013519 translation Methods 0.000 description 1
210000002700 urine Anatomy 0.000 description 1
238000012800 visualization Methods 0.000 description 1

Landscapes

Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

CA000546847A 1983-05-05 1984-03-30 Composition a base de sequences de polynucleotides visant a detecter un analyte Expired - Lifetime CA1341365C (fr)

Applications Claiming Priority (3)

Application Number	Priority Date	Filing Date	Title
US49192983A	1983-05-05	1983-05-05
US491,929		1983-05-05
CA000451043A CA1228811A (fr)	1983-05-05	1984-03-30	Methode d'analyse utilisant des sequences de polynucleotides

Related Parent Applications (1)

Application Number	Title	Priority Date	Filing Date
CA000451043A Division CA1228811A (fr)	1983-05-05	1984-03-30	Methode d'analyse utilisant des sequences de polynucleotides

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Publication Number	Publication Date
CA1341365C true CA1341365C (fr)	2002-06-04

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Date	Code	Title	Description
2019-08-06	MKEX	Expiry	Effective date: 20190604

Publication	Publication Date	Title
EP0611828B1 (fr)	2000-11-08	Composition pour l'utilisation dans un procédé de test utilisant des séquences polynucléotidiques
US4775619A (en)	1988-10-04	Polynucleotide determination with selectable cleavage sites
EP0360940B1 (fr)	1996-01-31	Détermination de polynucléotides aux sites de scission sélectables
US4824775A (en)	1989-04-25	Cells labeled with multiple Fluorophores bound to a nucleic acid carrier
EP0163220B1 (fr)	1990-06-20	Essai d'hybridation d'un acide nucléique au moyen d'anticorps anti-hybrides détectables
EP0117440B1 (fr)	1993-04-07	Méthodes et structures utilisant des sondes de polynucléotides marqués chimiquement et non-radioactifs
CA1234061A (fr)	1988-03-15	Adn marque
EP0703296B1 (fr)	1998-07-22	Détermination de polynucléotides par substitution d'un brin sur une sonde de capture
EP0664339A1 (fr)	1995-07-26	Methode de discrimination des acides nucleiques et necessaire d'essai a cette fin
US4670380A (en)	1987-06-02	Assays utilizing labeled nucleic acid probes
JPS61195699A (ja)	1986-08-29	ポリヌクレオチド配列を検出するための溶液相二重交雑アツセイ
JPS60237361A (ja)	1985-11-26	目標遺伝物質の検出方法
EP0131830A1 (fr)	1985-01-23	Cordons d'acides nucléiques marqués et produits d'addition pour leur préparation
SE500262C2 (sv)	1994-05-24	Ett förbättrat sätt för analys av nukleinsyror, en reagenskombination och en utrustning därför
CA2005557A1 (fr)	1990-06-16	Preparation chimique de sondes nucleotidiques ayant une activite hautement specifique
CA1238575A (fr)	1988-06-28	Hybridation de l'acide nucleique au moyen d'anticorps contre les complexes d'insertion
EP0146039A2 (fr)	1985-06-26	Test d'hybridation avec immobilisation d'hybrides par liaison antihybride
CA2202480A1 (fr)	1996-04-25	Marquage de polynucleotides par porphyrines
JPS61502889A (ja)	1986-12-11	化学的にラベルした刻酸、その利用法およびその実行用キット
US6027879A (en)	2000-02-22	Detection and isolation of nucleic acid sequences using a bifunctional hybridization probe
CA1341365C (fr)	2002-06-04	Composition a base de sequences de polynucleotides visant a detecter un analyte
US5783387A (en)	1998-07-21	Method for identifying and quantifying nucleic acid sequence aberrations
EP0209702A1 (fr)	1987-01-28	Essai d'hybridation en phase solide utilisant des anticorps antihybrides
JPS63282659A (ja)	1988-11-18	核酸の測定方法および測定剤
JPS60201257A (ja)	1985-10-11	特定のポリヌクレオチド配列順序の検出方法