CA1341617C - Inhibine extracte de liquide folliculaire ovarien - Google Patents

Inhibine extracte de liquide folliculaire ovarien

Info

Publication number
CA1341617C
CA1341617C CA482470A CA482470A CA1341617C CA 1341617 C CA1341617 C CA 1341617C CA 482470 A CA482470 A CA 482470A CA 482470 A CA482470 A CA 482470A CA 1341617 C CA1341617 C CA 1341617C
Authority
CA
Canada
Prior art keywords
inhibin
protein
mammal
antiserum
sub
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA482470A
Other languages
English (en)
Inventor
Henry George Burger
David Moritz De Kretser
John Kerr Findlay
Francis Joseph Morgan
Milton Thomas William Hearn
David Mark Robertson
Robert Gregory Forage
Richard Edward Hugh Wettenhall
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Monash Medical Centre
St Vincents Institute of Medical Research
Inhibin Pty Ltd
Monash University
Original Assignee
Monash Medical Centre
St Vincents Institute of Medical Research
Inhibin Pty Ltd
Monash University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Monash Medical Centre, St Vincents Institute of Medical Research, Inhibin Pty Ltd, Monash University filed Critical Monash Medical Centre
Application granted granted Critical
Publication of CA1341617C publication Critical patent/CA1341617C/fr
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/689—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to pregnancy or the gonads
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/26—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against hormones ; against hormone releasing or inhibiting factors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/815—Test for named compound or class of compounds
    • Y10S436/817—Steroids or hormones
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/806—Antigenic peptides or proteins
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/827—Proteins from mammals or birds
    • Y10S530/85—Reproductive organs or embryos

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Endocrinology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Food Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Toxicology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Microbiology (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Reproductive Health (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Steroid Compounds (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Networks Using Active Elements (AREA)
  • Centrifugal Separators (AREA)
  • External Artificial Organs (AREA)

Abstract

Une protéine qui répond à tous les critères biologiques qui sont caractéristiques de l'inhibine a été isolée d'une source gonadique. La purification et la caractérisation d'inhibine et l'utilisation de la matière purifiée pour produire des anticorps, l'utilisation d'inhibine et dudit antisérum dans un dosage radio-dosage quantitatif, et les applications in vitro et in vivo d'anticorps d'inhibine et de l'inhibine, sont décrites. Il est fourni une protéine purifiée, l'inhibine, caractérisé en ce que : a. le poids moléculaire apparent tel que déterminé par SDS-PAGE est de 56.000 ~ 1.000 b. le point isoélectrique est compris entre 6.9 et 7.3 c. la protéine peut se lier spécifiquement à la concanavaline A - Sepharose d. la protéine est constituée de deux sous-unités, caractérisées en ce que: i. leurs poids moléculaires apparents comme déterminé par SDS-PAGE sont respectivement de 44.000 ~ 3.000 et 14.000 ~ 2.000. ii. le point isoélectrique de la sous-unité de poids moléculaire 44 000 est dans la plage de 6,0 à 7,0 iii. les séquences d'acides aminés N-terminales des deux sous-unités sont telles que décrites ici e. la protéine peut supprimer l'hormone folliculo-stimulante (FSH), mais pas l'hormone lutéinisante (LH), l'hormone stimulant la thyroïde ou la prolactine dans un système de dosage biologique in vitro f. la protéine peut être marquée à l'iode radioactif. Il est également prévu un procédé pour isoler et purifier l'inhibine de liquide folliculaire ovarien de mammifère, caractérisé par a. une ou plusieurs étapes de chromatographie par perméation de gel, b. une ou plusieurs étapes de chromatographie liquide haute performance en phase inversée ; c. une ou plusieurs étapes de préparation polyacrylamide d'électrophorèse sur gel  ; d. une élution électrophorétique de l'inhibine purifiée.
CA482470A 1984-06-08 1985-05-27 Inhibine extracte de liquide folliculaire ovarien Expired - Fee Related CA1341617C (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
AUPG542784 1984-06-08
AUPG5427 1984-06-08
AUPH003185 1985-04-04
AUPH00031 1985-04-04

Publications (1)

Publication Number Publication Date
CA1341617C true CA1341617C (fr) 2011-06-28

Family

ID=25642809

Family Applications (1)

Application Number Title Priority Date Filing Date
CA482470A Expired - Fee Related CA1341617C (fr) 1984-06-08 1985-05-27 Inhibine extracte de liquide folliculaire ovarien

Country Status (10)

Country Link
US (2) US5102807A (fr)
EP (1) EP0185034B1 (fr)
JP (2) JP2521707B2 (fr)
AT (1) ATE95194T1 (fr)
CA (1) CA1341617C (fr)
DE (1) DE3587603T2 (fr)
DK (1) DK171711B1 (fr)
NO (1) NO176570C (fr)
NZ (1) NZ212248A (fr)
WO (1) WO1986000078A1 (fr)

Families Citing this family (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1341617C (fr) * 1984-06-08 2011-06-28 Henry George Burger Inhibine extracte de liquide folliculaire ovarien
KR940004077B1 (ko) 1985-04-18 1994-05-11 바이오테크놀로지 오스트레일리아 프로프라이어터리 리밋티드 재조합 인히빈
US5089396A (en) * 1985-10-03 1992-02-18 Genentech, Inc. Nucleic acid encoding β chain prodomains of inhibin and method for synthesizing polypeptides using such nucleic acid
NZ217727A (en) * 1985-10-03 1990-05-28 Genentech Inc Nucleic acid encoding alpha or b chain of inhibin, its production and compositions containing it
US4798885A (en) * 1986-02-07 1989-01-17 Genentech, Inc. Compositions of hormonally active human and porcine inhibin containing an α chain and 62 chain
US5215893A (en) * 1985-10-03 1993-06-01 Genentech, Inc. Nucleic acid encoding the ba chain prodomains of inhibin and method for synthesizing polypeptides using such nucleic acid
US4864019A (en) * 1985-11-08 1989-09-05 The Salk Institute For Biological Studies Antibodies to inhibin and conjugates produced therefrom
NZ219609A (en) * 1986-03-13 1990-09-26 Univ Monash Assay methods for inhibin
US5015729A (en) * 1986-06-24 1991-05-14 The Salk Institute For Biological Studies Ovine inhibin
US6150328A (en) * 1986-07-01 2000-11-21 Genetics Institute, Inc. BMP products
WO1988005789A1 (fr) * 1987-01-29 1988-08-11 Biotechnology Australia Pty. Ltd. Isolement a partir de fluide folliculaire de proteines a chaine unique avec activite de suppression de la fsh
US5032507A (en) * 1987-11-13 1991-07-16 The Salk Institute For Biological Studies Potentiation of erythropoiesis
US5196192A (en) * 1988-05-31 1993-03-23 Biotechnology Australia Pty. Ltd. Actions of hormones
WO1991010445A1 (fr) * 1990-01-08 1991-07-25 Genentech, Inc. Procede destine a accroitre la fertilite feminine
DE4000327A1 (de) * 1990-01-08 1991-07-11 Stefan Heiden Arzneimittel zur verbesserung der ovarreaktion und der eizell- und embryonen- produktion bei haussaeugetieren
US5942220A (en) * 1990-03-16 1999-08-24 Chiron Corporation Inhibitor of cytokine activity and applications thereof
KR100259827B1 (ko) 1991-11-04 2000-06-15 브루스 엠. 에이센, 토마스 제이 데스로저 재조합 골형태 형성 단백질 헤테로다이머, 그 조성물 및 사용방법
US20080070842A1 (en) * 1991-11-04 2008-03-20 David Israel Recombinant bone morphogenetic protein heterodimers, compositions and methods of use
US5428011A (en) * 1992-06-16 1995-06-27 Procyon Biopharma, Inc. Pharmaceutical preparations for inhibiting tumours associated with prostate adenocarcinoma
EP0698094B1 (fr) 1993-05-12 2004-02-04 Genetics Institute, LLC Compositions de bmp-11
US6291206B1 (en) * 1993-09-17 2001-09-18 Genetics Institute, Inc. BMP receptor proteins
EP0733109B9 (fr) * 1993-12-07 2006-07-05 Genetics Institute, LLC Bmp-12, bmp-13 et compositions de celles-ci pour l'induction de tendons
US5786179A (en) * 1994-02-28 1998-07-28 Agritech Technologies Ltd. Heterologous protein comprising avian alpha-subunit inhibin protein and methods of producing same
US20030171283A1 (en) * 1994-02-28 2003-09-11 Satterlee Daniel G. Inhibin compositions and methods of enhancing production performance
US5747659A (en) * 1994-02-28 1998-05-05 Agritech Technologies, Ltd. Fusion gene products encoding avian alpha subunit inhibin protein, or an immunogenic fragment thereof, and a carrier protein
AUPO638897A0 (en) * 1997-04-23 1997-05-22 Monash University Modulation of cell growth and methods relating thereto
AU780982B2 (en) * 1997-04-23 2005-04-28 Monash University Inhibin modulation of cell growth
TW586934B (en) * 1997-05-19 2004-05-11 Sumitomo Pharma Immunopotentiating composition
US6727224B1 (en) * 1999-02-01 2004-04-27 Genetics Institute, Llc. Methods and compositions for healing and repair of articular cartilage
EP1223990B1 (fr) 1999-10-15 2004-07-28 Genetics Institute, LLC Formulations a base d'acide hyaluronique aux fins de l'administration de proteines osteogenes
CA2355334A1 (fr) * 2000-10-16 2002-04-16 Procyon Biopharma Inc. Preparations pharmaceutiques et methodes visant a inhiber la croissance de tumeurs
US20030082233A1 (en) * 2000-12-01 2003-05-01 Lyons Karen M. Method and composition for modulating bone growth
TWI329129B (en) * 2001-02-08 2010-08-21 Wyeth Corp Modified and stabilized gdf propeptides and uses thereof
US7226587B2 (en) * 2001-06-01 2007-06-05 Wyeth Compositions and methods for systemic administration of sequences encoding bone morphogenetic proteins
TWI267378B (en) * 2001-06-08 2006-12-01 Wyeth Corp Calcium phosphate delivery vehicles for osteoinductive proteins
US20050026833A1 (en) * 2001-11-08 2005-02-03 Rabbani Shafaat Ahmed PSP-94: use for treatment of hypercalcemia and bone metastasis
WO2003099992A2 (fr) * 2002-05-17 2003-12-04 Wyeth Vehicules injectables solides contenant de l'acide hyaluronique pour l'administration de proteines osteogenes
AU2003297254A1 (en) * 2002-11-07 2004-06-03 Agritech Technology, Ltd Novel compositions that enhance production performance in avians
TW200518767A (en) * 2003-09-12 2005-06-16 Wyeth Corp Injectable calcium phosphate solid rods and pastes for delivery of osteogenic proteins
ES2667013T3 (es) 2010-11-29 2018-05-09 Mote Marine Laboratory, Inc. Determinación de las características sexuales de peces usando hormonas peptídicas
BR102014005376A2 (pt) * 2014-03-07 2016-02-10 Ouro Fino Saúde Animal Ltda antígeno de inibina alfa, gene codificante de inibina alfa, gene codificante de proteína de fusão, processo de obtenção do antígeno de inibina alfa, composição antigênica, uso do antígeno de inibina alfa e uso da composição antigênica

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FR2361912A1 (fr) * 1976-08-19 1978-03-17 Merck & Co Inc Vaccin anti-fecondite a base de membrane pellucide solubilisee
US4409139A (en) * 1981-08-10 1983-10-11 The Salk Institute For Biological Studies Gonadostatin
US4423037A (en) * 1982-05-13 1983-12-27 The General Hospital Corporation Inhibitors of peptide hormone action
CA1341617C (fr) * 1984-06-08 2011-06-28 Henry George Burger Inhibine extracte de liquide folliculaire ovarien
KR940004077B1 (ko) * 1985-04-18 1994-05-11 바이오테크놀로지 오스트레일리아 프로프라이어터리 리밋티드 재조합 인히빈
US4624944A (en) * 1985-06-14 1986-11-25 The Regents Of The University Of California Human seminal alpha-inhibins
US4740587A (en) * 1985-07-18 1988-04-26 The Salk Institute For Biological Studies Inhibin and method of purifying same
US4798885A (en) * 1986-02-07 1989-01-17 Genentech, Inc. Compositions of hormonally active human and porcine inhibin containing an α chain and 62 chain
US4864019A (en) * 1985-11-08 1989-09-05 The Salk Institute For Biological Studies Antibodies to inhibin and conjugates produced therefrom
US4737578A (en) * 1986-02-10 1988-04-12 The Salk Institute For Biological Studies Human inhibin
US5196192A (en) * 1988-05-31 1993-03-23 Biotechnology Australia Pty. Ltd. Actions of hormones

Also Published As

Publication number Publication date
JP2521707B2 (ja) 1996-08-07
EP0185034A4 (fr) 1988-01-28
NO176570C (no) 1995-04-26
JPS61502399A (ja) 1986-10-23
DE3587603D1 (de) 1993-11-04
JP2583030B2 (ja) 1997-02-19
WO1986000078A1 (fr) 1986-01-03
US5364837A (en) 1994-11-15
DK171711B1 (da) 1997-04-01
NZ212248A (en) 1991-05-28
EP0185034B1 (fr) 1993-09-29
NO176570B (no) 1995-01-16
DE3587603T2 (de) 1994-02-10
DK60586D0 (da) 1986-02-07
US5102807A (en) 1992-04-07
EP0185034A1 (fr) 1986-06-25
DK60586A (da) 1986-02-07
JPH0848636A (ja) 1996-02-20
NO860427L (no) 1986-02-06
ATE95194T1 (de) 1993-10-15

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Legal Events

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MKLA Lapsed

Effective date: 20210628