CA2016518A1 - Systeme de diagnostic a base d'acide nucleique pour la detection de pneumocystis carinii dans les produits sanguins - Google Patents

Systeme de diagnostic a base d'acide nucleique pour la detection de pneumocystis carinii dans les produits sanguins

Info

Publication number: CA2016518A1
Authority: CA; Canada
Prior art keywords: nucleic acid; carinii; polynucleotide; pneumocystis carinii; polynucleotides
Prior art date: 1989-05-11
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Abandoned

Application number

CA 2016518

Other languages

English (en)

Inventor

Peter Baram

Kary B. Mullis

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Xytronyx Inc

Original Assignee

Xytronyx Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1989-05-11

Filing date

1990-05-10

Publication date

1990-11-11

1990-05-10 Application filed by Xytronyx Inc filed Critical Xytronyx Inc

1990-11-11 Publication of CA2016518A1 publication Critical patent/CA2016518A1/fr

Status Abandoned legal-status Critical Current

Links

241000233872 Pneumocystis carinii Species 0.000 title claims abstract description 100
150000007523 nucleic acids Chemical class 0.000 title claims abstract description 90
102000039446 nucleic acids Human genes 0.000 title claims abstract description 72
108020004707 nucleic acids Proteins 0.000 title claims abstract description 72
238000000034 method Methods 0.000 title claims abstract description 52
238000001514 detection method Methods 0.000 title description 26
239000010836 blood and blood product Substances 0.000 title description 2
229940125691 blood product Drugs 0.000 title description 2
206010035660 Pneumocystis Infections Diseases 0.000 claims abstract description 18
241000124008 Mammalia Species 0.000 claims abstract description 15
206010073756 Pneumocystis jirovecii infection Diseases 0.000 claims abstract description 6
108091033319 polynucleotide Proteins 0.000 claims description 99
102000040430 polynucleotide Human genes 0.000 claims description 99
239000002157 polynucleotide Substances 0.000 claims description 98
239000000523 sample Substances 0.000 claims description 79
239000002773 nucleotide Substances 0.000 claims description 38
125000003729 nucleotide group Chemical group 0.000 claims description 38
230000000295 complement effect Effects 0.000 claims description 22
108091028043 Nucleic acid sequence Proteins 0.000 claims description 21
108020004418 ribosomal RNA Proteins 0.000 claims description 14
239000012530 fluid Substances 0.000 claims description 12
230000002792 vascular Effects 0.000 claims description 12
239000000203 mixture Substances 0.000 claims description 9
230000003321 amplification Effects 0.000 claims description 6
238000003199 nucleic acid amplification method Methods 0.000 claims description 6
241000282414 Homo sapiens Species 0.000 claims description 5
108020004414 DNA Proteins 0.000 claims description 3
108091032973 (ribonucleotides)n+m Proteins 0.000 claims 1
238000012408 PCR amplification Methods 0.000 claims 1
238000003752 polymerase chain reaction Methods 0.000 abstract description 36
210000004369 blood Anatomy 0.000 abstract description 11
239000008280 blood Substances 0.000 abstract description 11
238000007899 nucleic acid hybridization Methods 0.000 abstract 1
239000000047 product Substances 0.000 description 33
238000009396 hybridization Methods 0.000 description 30
CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 21
238000006243 chemical reaction Methods 0.000 description 20
229920002477 rna polymer Polymers 0.000 description 15
210000004072 lung Anatomy 0.000 description 13
108020004635 Complementary DNA Proteins 0.000 description 12
241000700159 Rattus Species 0.000 description 12
238000010804 cDNA synthesis Methods 0.000 description 12
239000002299 complementary DNA Substances 0.000 description 12
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 11
208000031513 cyst Diseases 0.000 description 9
206010035664 Pneumonia Diseases 0.000 description 7
241000589500 Thermus aquaticus Species 0.000 description 7
239000000872 buffer Substances 0.000 description 7
239000000243 solution Substances 0.000 description 7
239000000427 antigen Substances 0.000 description 6
102000036639 antigens Human genes 0.000 description 6
108091007433 antigens Proteins 0.000 description 6
230000015572 biosynthetic process Effects 0.000 description 6
208000015181 infectious disease Diseases 0.000 description 6
210000002966 serum Anatomy 0.000 description 6
210000001519 tissue Anatomy 0.000 description 6
238000003556 assay Methods 0.000 description 5
150000001875 compounds Chemical class 0.000 description 5
201000010099 disease Diseases 0.000 description 5
208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 5
239000000499 gel Substances 0.000 description 5
238000002360 preparation method Methods 0.000 description 5
230000002285 radioactive effect Effects 0.000 description 5
230000000717 retained effect Effects 0.000 description 5
238000000926 separation method Methods 0.000 description 5
238000003786 synthesis reaction Methods 0.000 description 5
YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 4
239000002253 acid Substances 0.000 description 4
ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 4
229960005542 ethidium bromide Drugs 0.000 description 4
229910052747 lanthanoid Inorganic materials 0.000 description 4
150000002602 lanthanoids Chemical group 0.000 description 4
239000011159 matrix material Substances 0.000 description 4
OSBLTNPMIGYQGY-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;2-[2-[bis(carboxymethyl)amino]ethyl-(carboxymethyl)amino]acetic acid;boric acid Chemical compound OB(O)O.OCC(N)(CO)CO.OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O OSBLTNPMIGYQGY-UHFFFAOYSA-N 0.000 description 3
108010014303 DNA-directed DNA polymerase Proteins 0.000 description 3
102000016928 DNA-directed DNA polymerase Human genes 0.000 description 3
KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 3
102100031780 Endonuclease Human genes 0.000 description 3
108091034117 Oligonucleotide Proteins 0.000 description 3
208000005384 Pneumocystis Pneumonia Diseases 0.000 description 3
108010092799 RNA-directed DNA polymerase Proteins 0.000 description 3
HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
239000008051 TBE buffer Substances 0.000 description 3
238000001502 gel electrophoresis Methods 0.000 description 3
239000000463 material Substances 0.000 description 3
239000013642 negative control Substances 0.000 description 3
150000003839 salts Chemical class 0.000 description 3
FVAUCKIRQBBSSJ-UHFFFAOYSA-M sodium iodide Chemical compound [Na+].[I-] FVAUCKIRQBBSSJ-UHFFFAOYSA-M 0.000 description 3
238000012800 visualization Methods 0.000 description 3
208000030507 AIDS Diseases 0.000 description 2
241000713838 Avian myeloblastosis virus Species 0.000 description 2
OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
206010011732 Cyst Diseases 0.000 description 2
102000053602 DNA Human genes 0.000 description 2
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
206010062016 Immunosuppression Diseases 0.000 description 2
TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
239000000020 Nitrocellulose Substances 0.000 description 2
206010035742 Pneumonitis Diseases 0.000 description 2
206010036790 Productive cough Diseases 0.000 description 2
DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 2
238000002105 Southern blotting Methods 0.000 description 2
108010006785 Taq Polymerase Proteins 0.000 description 2
FJWGYAHXMCUOOM-QHOUIDNNSA-N [(2s,3r,4s,5r,6r)-2-[(2r,3r,4s,5r,6s)-4,5-dinitrooxy-2-(nitrooxymethyl)-6-[(2r,3r,4s,5r,6s)-4,5,6-trinitrooxy-2-(nitrooxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-3,5-dinitrooxy-6-(nitrooxymethyl)oxan-4-yl] nitrate Chemical compound O([C@@H]1O[C@@H]([C@H]([C@H](O[N+]([O-])=O)[C@H]1O[N+]([O-])=O)O[C@H]1[C@@H]([C@@H](O[N+]([O-])=O)[C@H](O[N+]([O-])=O)[C@@H](CO[N+]([O-])=O)O1)O[N+]([O-])=O)CO[N+](=O)[O-])[C@@H]1[C@@H](CO[N+]([O-])=O)O[C@@H](O[N+]([O-])=O)[C@H](O[N+]([O-])=O)[C@H]1O[N+]([O-])=O FJWGYAHXMCUOOM-QHOUIDNNSA-N 0.000 description 2
239000011543 agarose gel Substances 0.000 description 2
238000000246 agarose gel electrophoresis Methods 0.000 description 2
BTBJBAZGXNKLQC-UHFFFAOYSA-N ammonium lauryl sulfate Chemical compound [NH4+].CCCCCCCCCCCCOS([O-])(=O)=O BTBJBAZGXNKLQC-UHFFFAOYSA-N 0.000 description 2
229940063953 ammonium lauryl sulfate Drugs 0.000 description 2
238000013459 approach Methods 0.000 description 2
239000011616 biotin Substances 0.000 description 2
229960002685 biotin Drugs 0.000 description 2
235000020958 biotin Nutrition 0.000 description 2
229910052799 carbon Inorganic materials 0.000 description 2
230000003196 chaotropic effect Effects 0.000 description 2
239000013522 chelant Substances 0.000 description 2
239000003153 chemical reaction reagent Substances 0.000 description 2
239000003795 chemical substances by application Substances 0.000 description 2
239000008139 complexing agent Substances 0.000 description 2
OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 2
SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 description 2
SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 description 2
RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 description 2
HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 description 2
NHVNXKFIZYSCEB-XLPZGREQSA-N dTTP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C1 NHVNXKFIZYSCEB-XLPZGREQSA-N 0.000 description 2
238000004925 denaturation Methods 0.000 description 2
230000036425 denaturation Effects 0.000 description 2
239000005547 deoxyribonucleotide Substances 0.000 description 2
125000002637 deoxyribonucleotide group Chemical group 0.000 description 2
238000009795 derivation Methods 0.000 description 2
238000003745 diagnosis Methods 0.000 description 2
239000003651 drinking water Substances 0.000 description 2
235000020188 drinking water Nutrition 0.000 description 2
238000001962 electrophoresis Methods 0.000 description 2
230000002255 enzymatic effect Effects 0.000 description 2
UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 2
230000001506 immunosuppresive effect Effects 0.000 description 2
238000002955 isolation Methods 0.000 description 2
239000012160 loading buffer Substances 0.000 description 2
238000012423 maintenance Methods 0.000 description 2
229910052751 metal Inorganic materials 0.000 description 2
239000002184 metal Substances 0.000 description 2
229920001220 nitrocellulos Polymers 0.000 description 2
229940079938 nitrocellulose Drugs 0.000 description 2
239000002777 nucleoside Substances 0.000 description 2
239000003960 organic solvent Substances 0.000 description 2
239000008188 pellet Substances 0.000 description 2
150000002972 pentoses Chemical class 0.000 description 2
239000002953 phosphate buffered saline Substances 0.000 description 2
239000013641 positive control Substances 0.000 description 2
229960005205 prednisolone Drugs 0.000 description 2
OIGNJSKKLXVSLS-VWUMJDOOSA-N prednisolone Chemical compound O=C1C=C[C@]2(C)[C@H]3[C@@H](O)C[C@](C)([C@@](CC4)(O)C(=O)CO)[C@@H]4[C@@H]3CCC2=C1 OIGNJSKKLXVSLS-VWUMJDOOSA-N 0.000 description 2
108090000623 proteins and genes Proteins 0.000 description 2
230000035945 sensitivity Effects 0.000 description 2
208000024794 sputum Diseases 0.000 description 2
210000003802 sputum Anatomy 0.000 description 2
239000008223 sterile water Substances 0.000 description 2
UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
239000000126 substance Substances 0.000 description 2
239000004094 surface-active agent Substances 0.000 description 2
208000024891 symptom Diseases 0.000 description 2
RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 description 2
239000013598 vector Substances 0.000 description 2
QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
229930024421 Adenine Natural products 0.000 description 1
GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 1
APKFDSVGJQXUKY-KKGHZKTASA-N Amphotericin-B Natural products O[C@H]1[C@@H](N)[C@H](O)[C@@H](C)O[C@H]1O[C@H]1C=CC=CC=CC=CC=CC=CC=C[C@H](C)[C@@H](O)[C@@H](C)[C@H](C)OC(=O)C[C@H](O)C[C@H](O)CC[C@@H](O)[C@H](O)C[C@H](O)C[C@](O)(C[C@H](O)[C@H]2C(O)=O)O[C@H]2C1 APKFDSVGJQXUKY-KKGHZKTASA-N 0.000 description 1
208000035143 Bacterial infection Diseases 0.000 description 1
229920000742 Cotton Polymers 0.000 description 1
206010011224 Cough Diseases 0.000 description 1
206010011703 Cyanosis Diseases 0.000 description 1
238000009007 Diagnostic Kit Methods 0.000 description 1
QXNVGIXVLWOKEQ-UHFFFAOYSA-N Disodium Chemical compound [Na][Na] QXNVGIXVLWOKEQ-UHFFFAOYSA-N 0.000 description 1
208000000059 Dyspnea Diseases 0.000 description 1
206010013975 Dyspnoeas Diseases 0.000 description 1
102000004190 Enzymes Human genes 0.000 description 1
108090000790 Enzymes Proteins 0.000 description 1
241000588724 Escherichia coli Species 0.000 description 1
241000282412 Homo Species 0.000 description 1
206010061598 Immunodeficiency Diseases 0.000 description 1
208000032420 Latent Infection Diseases 0.000 description 1
241001465754 Metazoa Species 0.000 description 1
102000007474 Multiprotein Complexes Human genes 0.000 description 1
108010085220 Multiprotein Complexes Proteins 0.000 description 1
208000001388 Opportunistic Infections Diseases 0.000 description 1
229910019142 PO4 Inorganic materials 0.000 description 1
229930182555 Penicillin Natural products 0.000 description 1
JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 1
241000233870 Pneumocystis Species 0.000 description 1
206010037660 Pyrexia Diseases 0.000 description 1
-1 RNA or DNA Chemical class 0.000 description 1
108020004511 Recombinant DNA Proteins 0.000 description 1
102000006382 Ribonucleases Human genes 0.000 description 1
108010083644 Ribonucleases Proteins 0.000 description 1
108091028664 Ribonucleotide Proteins 0.000 description 1
240000004808 Saccharomyces cerevisiae Species 0.000 description 1
235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 1
229930006000 Sucrose Natural products 0.000 description 1
CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
239000007983 Tris buffer Substances 0.000 description 1
MJHYBJOMJPGNMM-KGWLDMEJSA-N [2-[(8s,9s,10r,11s,13s,14s,17r)-11,17-dihydroxy-10,13-dimethyl-3-oxo-7,8,9,11,12,14,15,16-octahydro-6h-cyclopenta[a]phenanthren-17-yl]-2-oxoethyl] 4-(nitrooxymethyl)benzoate Chemical compound O=C([C@@]1(O)CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)[C@@H](O)C[C@@]21C)COC(=O)C1=CC=C(CO[N+]([O-])=O)C=C1 MJHYBJOMJPGNMM-KGWLDMEJSA-N 0.000 description 1
JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 1
150000007513 acids Chemical class 0.000 description 1
229960000643 adenine Drugs 0.000 description 1
239000000908 ammonium hydroxide Substances 0.000 description 1
APKFDSVGJQXUKY-INPOYWNPSA-N amphotericin B Chemical compound O[C@H]1[C@@H](N)[C@H](O)[C@@H](C)O[C@H]1O[C@H]1/C=C/C=C/C=C/C=C/C=C/C=C/C=C/[C@H](C)[C@@H](O)[C@@H](C)[C@H](C)OC(=O)C[C@H](O)C[C@H](O)CC[C@@H](O)[C@H](O)C[C@H](O)C[C@](O)(C[C@H](O)[C@H]2C(O)=O)O[C@H]2C1 APKFDSVGJQXUKY-INPOYWNPSA-N 0.000 description 1
229960003942 amphotericin b Drugs 0.000 description 1
238000004458 analytical method Methods 0.000 description 1
125000000129 anionic group Chemical group 0.000 description 1
239000003945 anionic surfactant Substances 0.000 description 1
239000012062 aqueous buffer Substances 0.000 description 1
239000003125 aqueous solvent Substances 0.000 description 1
125000003118 aryl group Chemical group 0.000 description 1
230000001580 bacterial effect Effects 0.000 description 1
208000022362 bacterial infectious disease Diseases 0.000 description 1
230000005250 beta ray Effects 0.000 description 1
230000002146 bilateral effect Effects 0.000 description 1
238000001574 biopsy Methods 0.000 description 1
239000001045 blue dye Substances 0.000 description 1
210000001185 bone marrow Anatomy 0.000 description 1
KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 1
239000004327 boric acid Substances 0.000 description 1
UDSAIICHUKSCKT-UHFFFAOYSA-N bromophenol blue Chemical compound C1=C(Br)C(O)=C(Br)C=C1C1(C=2C=C(Br)C(O)=C(Br)C=2)C2=CC=CC=C2S(=O)(=O)O1 UDSAIICHUKSCKT-UHFFFAOYSA-N 0.000 description 1
XMEVHPAGJVLHIG-FMZCEJRJSA-N chembl454950 Chemical compound [Cl-].C1=CC=C2[C@](O)(C)[C@H]3C[C@H]4[C@H]([NH+](C)C)C(O)=C(C(N)=O)C(=O)[C@@]4(O)C(O)=C3C(=O)C2=C1O XMEVHPAGJVLHIG-FMZCEJRJSA-N 0.000 description 1
239000007795 chemical reaction product Substances 0.000 description 1
238000010367 cloning Methods 0.000 description 1
239000013599 cloning vector Substances 0.000 description 1
238000012864 cross contamination Methods 0.000 description 1
229940104302 cytosine Drugs 0.000 description 1
230000034994 death Effects 0.000 description 1
231100000517 death Toxicity 0.000 description 1
230000001419 dependent effect Effects 0.000 description 1
238000007865 diluting Methods 0.000 description 1
LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 1
208000037771 disease arising from reactivation of latent virus Diseases 0.000 description 1
229940079593 drug Drugs 0.000 description 1
239000003814 drug Substances 0.000 description 1
150000002148 esters Chemical class 0.000 description 1
GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 1
239000012634 fragment Substances 0.000 description 1
230000002538 fungal effect Effects 0.000 description 1
230000002496 gastric effect Effects 0.000 description 1
238000007429 general method Methods 0.000 description 1
PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical compound [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 description 1
ZJYYHGLJYGJLLN-UHFFFAOYSA-N guanidinium thiocyanate Chemical compound SC#N.NC(N)=N ZJYYHGLJYGJLLN-UHFFFAOYSA-N 0.000 description 1
229910052739 hydrogen Inorganic materials 0.000 description 1
239000001257 hydrogen Substances 0.000 description 1
238000000338 in vitro Methods 0.000 description 1
238000011065 in-situ storage Methods 0.000 description 1
238000010348 incorporation Methods 0.000 description 1
229910001629 magnesium chloride Inorganic materials 0.000 description 1
159000000003 magnesium salts Chemical class 0.000 description 1
239000003550 marker Substances 0.000 description 1
238000002844 melting Methods 0.000 description 1
230000008018 melting Effects 0.000 description 1
238000012986 modification Methods 0.000 description 1
230000004048 modification Effects 0.000 description 1
238000010369 molecular cloning Methods 0.000 description 1
150000003833 nucleoside derivatives Chemical class 0.000 description 1
125000003835 nucleoside group Chemical group 0.000 description 1
210000000056 organ Anatomy 0.000 description 1
244000052769 pathogen Species 0.000 description 1
230000001717 pathogenic effect Effects 0.000 description 1
229940049954 penicillin Drugs 0.000 description 1
125000001805 pentosyl group Chemical group 0.000 description 1
NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
239000010452 phosphate Substances 0.000 description 1
125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 description 1
239000013612 plasmid Substances 0.000 description 1
201000000317 pneumocystosis Diseases 0.000 description 1
229920002401 polyacrylamide Polymers 0.000 description 1
238000006116 polymerization reaction Methods 0.000 description 1
ONQDVAFWWYYXHM-UHFFFAOYSA-M potassium lauryl sulfate Chemical compound [K+].CCCCCCCCCCCCOS([O-])(=O)=O ONQDVAFWWYYXHM-UHFFFAOYSA-M 0.000 description 1
229940116985 potassium lauryl sulfate Drugs 0.000 description 1
239000000376 reactant Substances 0.000 description 1
239000012429 reaction media Substances 0.000 description 1
230000010076 replication Effects 0.000 description 1
210000002345 respiratory system Anatomy 0.000 description 1
108091008146 restriction endonucleases Proteins 0.000 description 1
PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 1
239000002336 ribonucleotide Substances 0.000 description 1
125000002652 ribonucleotide group Chemical group 0.000 description 1
235000009518 sodium iodide Nutrition 0.000 description 1
229950005425 sodium myristyl sulfate Drugs 0.000 description 1
UPUIQOIQVMNQAP-UHFFFAOYSA-M sodium;tetradecyl sulfate Chemical compound [Na+].CCCCCCCCCCCCCCOS([O-])(=O)=O UPUIQOIQVMNQAP-UHFFFAOYSA-M 0.000 description 1
241000894007 species Species 0.000 description 1
238000013222 sprague-dawley male rat Methods 0.000 description 1
238000010186 staining Methods 0.000 description 1
229960005322 streptomycin Drugs 0.000 description 1
239000005720 sucrose Substances 0.000 description 1
239000006228 supernatant Substances 0.000 description 1
239000000725 suspension Substances 0.000 description 1
229960002180 tetracycline Drugs 0.000 description 1
MPLHNVLQVRSVEE-UHFFFAOYSA-N texas red Chemical compound [O-]S(=O)(=O)C1=CC(S(Cl)(=O)=O)=CC=C1C(C1=CC=2CCCN3CCCC(C=23)=C1O1)=C2C1=C(CCC1)C3=[N+]1CCCC3=C2 MPLHNVLQVRSVEE-UHFFFAOYSA-N 0.000 description 1
229940113082 thymine Drugs 0.000 description 1
229950003937 tolonium Drugs 0.000 description 1
HNONEKILPDHFOL-UHFFFAOYSA-M tolonium chloride Chemical compound [Cl-].C1=C(C)C(N)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 HNONEKILPDHFOL-UHFFFAOYSA-M 0.000 description 1
LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
210000003812 trophozoite Anatomy 0.000 description 1
238000005406 washing Methods 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae

Landscapes

Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Analytical Chemistry (AREA)
Engineering & Computer Science (AREA)
Organic Chemistry (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Health & Medical Sciences (AREA)
Biotechnology (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Immunology (AREA)
Mycology (AREA)
Microbiology (AREA)
Molecular Biology (AREA)
Botany (AREA)
Biophysics (AREA)
Physics & Mathematics (AREA)
Biochemistry (AREA)
Bioinformatics & Cheminformatics (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

CA 2016518 1989-05-11 1990-05-10 Systeme de diagnostic a base d'acide nucleique pour la detection de pneumocystis carinii dans les produits sanguins Abandoned CA2016518A1 (fr)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US35093789A	1989-05-11	1989-05-11
US07/350,937		1989-05-11

Publications (1)

Publication Number	Publication Date
CA2016518A1 true CA2016518A1 (fr)	1990-11-11

Family

ID=23378835

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CA 2016518 Abandoned CA2016518A1 (fr)	1989-05-11	1990-05-10	Systeme de diagnostic a base d'acide nucleique pour la detection de pneumocystis carinii dans les produits sanguins

Country Status (8)

Country	Link
EP (1)	EP0471789A4 (fr)
JP (1)	JPH04505257A (fr)
AU (1)	AU5671190A (fr)
CA (1)	CA2016518A1 (fr)
GR (1)	GR900100354A (fr)
IE (1)	IE901720L (fr)
PT (1)	PT94001A (fr)
WO (1)	WO1990013669A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5643730A (en) *	1991-09-23	1997-07-01	Pfizer Inc.	Process for detecting specific mRNA and DNA in cells

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
GB8802667D0 (en) *	1988-02-05	1988-03-02	Chancellor Masters & Scholars	Probe for pneumoxystis carinii
DE69018961T2 (de) *	1989-08-11	1995-11-23	Amoco Corp	Detektion von pneumocystis carinii durch die verwendung von nukleinsäuresonden.
GB9012196D0 (en) *	1990-06-01	1990-07-18	Isis Innovation	Dna for diagnosing pneumocystis carinii
US5593836A (en) *	1993-05-14	1997-01-14	Niemiec; John T.	Primers and probes for detecting Pneumocystis carinii
US6180339B1 (en)	1995-01-13	2001-01-30	Bayer Corporation	Nucleic acid probes for the detection and identification of fungi
US5763169A (en) *	1995-01-13	1998-06-09	Chiron Diagnostics Corporation	Nucleic acid probes for the detection and identification of fungi

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US4683195A (en) *	1986-01-30	1987-07-28	Cetus Corporation	Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) *	1985-03-28	1987-07-28	Cetus Corporation	Process for amplifying nucleic acid sequences

1990
- 1990-05-10 CA CA 2016518 patent/CA2016518A1/fr not_active Abandoned
- 1990-05-10 PT PT9400190A patent/PT94001A/pt unknown
- 1990-05-11 AU AU56711/90A patent/AU5671190A/en not_active Abandoned
- 1990-05-11 JP JP2508214A patent/JPH04505257A/ja active Pending
- 1990-05-11 WO PCT/US1990/002633 patent/WO1990013669A1/fr not_active Ceased
- 1990-05-11 EP EP19900908762 patent/EP0471789A4/en not_active Withdrawn
- 1990-05-11 IE IE172090A patent/IE901720L/xx unknown
- 1990-05-11 GR GR900100354A patent/GR900100354A/el unknown

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5643730A (en) *	1991-09-23	1997-07-01	Pfizer Inc.	Process for detecting specific mRNA and DNA in cells

Also Published As

Publication number	Publication date
PT94001A (pt)	1991-02-08
AU5671190A (en)	1990-11-29
EP0471789A1 (fr)	1992-02-26
JPH04505257A (ja)	1992-09-17
IE901720L (en)	1990-11-11
EP0471789A4 (en)	1992-04-22
GR900100354A (el)	1991-10-10
WO1990013669A1 (fr)	1990-11-15

Legal Events

Date	Code	Title	Description
1992-11-10	FZDE	Dead

Publication	Publication Date	Title
US5935825A (en)	1999-08-10	Process and reagent for amplifying nucleic acid sequences
EP0875583B1 (fr)	2006-11-15	Amorces et trousse pour la detection de Chlamydia trachomatis
EP0824599B1 (fr)	2006-01-11	Cibles universelles pour identification d'especes
JP2798499B2 (ja)	1998-09-17	感染症診断用プローブ
EP0373960A2 (fr)	1990-06-20	Systéme auto-entretenu de réplication de séquences
EP0630971A2 (fr)	1994-12-28	Procédé, réactifs et kits de tests pour détection de neisseria gonorrhoea
EP0709467A2 (fr)	1996-05-01	Compositions et procédés pour la détection de chlamydia trachomatis
EP0687737A1 (fr)	1995-12-20	Détection du virus herpes simplex, treponema pallidum et haemophilus ducreyi
US20020102660A1 (en)	2002-08-01	Method for synthesis of nucleic acids
JP2023534457A5 (fr)	2024-07-26
US5968739A (en)	1999-10-19	Nucleic acid primers and probes for detecting Legionella pneumophila
AU5671190A (en)	1990-11-29	Nucleic acid based diagnostic system and process for the detection of (pneumocystis carinii) in blood products
US20220251630A1 (en)	2022-08-11	Polynucleotides for the amplification and detection of neisseria gonorrhoeae
US5545523A (en)	1996-08-13	Methods of detecting bovine herpesvirus 1 (BHV-1) in semen by nucleic acid amplification
CN112593014A (zh)	2021-04-02	一种检测猫上呼吸道疾病病原体的核酸组合物、试剂盒以及方法
AU716766B2 (en)	2000-03-09	Nucleic acid assay for the detection and differentiation of three chlamydia species
EP1176215B1 (fr)	2007-08-15	Amplification et détection de Mycoplasma pneumoniae
EP4269619A1 (fr)	2023-11-01	Composition pour diagnostiquer la maladie de lyme et kit la comprenant
JP3494509B2 (ja)	2004-02-09	核酸合成法
JP2787017B2 (ja)	1998-08-13	ミコバクテリア核酸の増幅および検出
EP0860503A1 (fr)	1998-08-26	Procede et materiau de detection de champignons
EP1809772B1 (fr)	2013-06-05	Compositions et methode de detection de streptocoques du groupe a
CN116479149A (zh)	2023-07-25	一组检测肺炎链球菌的引物探针及其检测试剂盒
JP3103882B2 (ja)	2000-10-30	バベシア・オバタに特異的な塩基配列とそれを利用したバベシア・オバタの検出方法
JP2004201636A (ja)	2004-07-22	病原真菌遺伝子の検出方法及び真菌の菌種同定用オリゴヌクレオチド

CA2016518A1 - Systeme de diagnostic a base d'acide nucleique pour la detection de pneumocystis carinii dans les produits sanguins - Google Patents

Info

Links

Classifications

Landscapes

Applications Claiming Priority (2)

Publications (1)

Family

ID=23378835

Family Applications (1)

Country Status (8)

Cited By (1)

Families Citing this family (6)

Family Cites Families (2)

Cited By (1)

Also Published As

Similar Documents

Legal Events