CA2088257A1 - Incorporation of selectably clevable and/or abasic sites into oligonucleotide chains and reagents therefor - Google Patents

Incorporation of selectably clevable and/or abasic sites into oligonucleotide chains and reagents therefor

Info

Publication number: CA2088257A1
Authority: CA; Canada
Prior art keywords: reagent; group; support; hydroxyl; polynucleotide
Prior art date: 1990-07-27
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Abandoned

Application number

CA 2088257

Other languages

English (en)

French (fr)

Inventor

Michael S. Urdea

Thomas Horn

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Novartis Vaccines and Diagnostics Inc

Original Assignee

Individual

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1990-07-27

Filing date

1991-07-25

Publication date

1992-01-28

1990-07-27 Priority claimed from US07/559,961 external-priority patent/US5430136A/en

1991-07-24 Priority claimed from US07/736,445 external-priority patent/US5367066A/en

1991-07-25 Application filed by Individual filed Critical Individual

1992-01-28 Publication of CA2088257A1 publication Critical patent/CA2088257A1/en

Status Abandoned legal-status Critical Current

Links

239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 131
108091034117 Oligonucleotide Proteins 0.000 title claims abstract description 64
208000035657 Abasia Diseases 0.000 title abstract description 17
238000010348 incorporation Methods 0.000 title description 9
239000002157 polynucleotide Substances 0.000 claims abstract description 48
108091033319 polynucleotide Proteins 0.000 claims abstract description 46
102000040430 polynucleotide Human genes 0.000 claims abstract description 46
125000002887 hydroxy group Chemical group [H]O* 0.000 claims abstract description 36
150000001875 compounds Chemical class 0.000 claims abstract description 24
238000009396 hybridization Methods 0.000 claims abstract description 14
-1 dimethoxytrityl Chemical group 0.000 claims description 55
238000003776 cleavage reaction Methods 0.000 claims description 43
230000007017 scission Effects 0.000 claims description 43
238000000034 method Methods 0.000 claims description 40
150000007523 nucleic acids Chemical class 0.000 claims description 31
102000039446 nucleic acids Human genes 0.000 claims description 30
108020004707 nucleic acids Proteins 0.000 claims description 30
108020004414 DNA Proteins 0.000 claims description 27
239000007787 solid Substances 0.000 claims description 26
238000003786 synthesis reaction Methods 0.000 claims description 20
125000001449 isopropyl group Chemical group [H]C([H])([H])C([H])(*)C([H])([H])[H] 0.000 claims description 19
150000008300 phosphoramidites Chemical group 0.000 claims description 19
125000003545 alkoxy group Chemical group 0.000 claims description 18
125000000217 alkyl group Chemical group 0.000 claims description 18
229910052739 hydrogen Inorganic materials 0.000 claims description 17
239000001257 hydrogen Substances 0.000 claims description 17
125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 claims description 17
125000000449 nitro group Chemical group [O-][N+](*)=O 0.000 claims description 17
125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims description 17
239000002777 nucleoside Substances 0.000 claims description 16
238000006303 photolysis reaction Methods 0.000 claims description 15
230000015843 photosynthesis, light reaction Effects 0.000 claims description 15
125000005843 halogen group Chemical group 0.000 claims description 14
150000003833 nucleoside derivatives Chemical class 0.000 claims description 14
239000012491 analyte Substances 0.000 claims description 11
125000003118 aryl group Chemical group 0.000 claims description 11
150000002431 hydrogen Chemical group 0.000 claims description 11
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RYYWUUFWQRZTIU-UHFFFAOYSA-N Thiophosphoric acid Chemical class OP(O)(S)=O RYYWUUFWQRZTIU-UHFFFAOYSA-N 0.000 claims description 8
125000003710 aryl alkyl group Chemical group 0.000 claims description 8
AQSJGOWTSHOLKH-UHFFFAOYSA-N phosphite(3-) Chemical class [O-]P([O-])[O-] AQSJGOWTSHOLKH-UHFFFAOYSA-N 0.000 claims description 8
150000004713 phosphodiesters Chemical class 0.000 claims description 8
OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical group [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims description 6
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239000000178 monomer Substances 0.000 claims description 5
230000000865 phosphorylative effect Effects 0.000 claims description 5
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Saccharide Compounds (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

CA 2088257 1990-07-27 1991-07-25 Incorporation of selectably clevable and/or abasic sites into oligonucleotide chains and reagents therefor Abandoned CA2088257A1 (en)

Applications Claiming Priority (3)

Application Number	Priority Date	Filing Date	Title
US559,961		1990-07-27
US07/559,961 US5430136A (en)	1984-10-16	1990-07-27	Oligonucleotides having selectably cleavable and/or abasic sites
US07/736,445 US5367066A (en)	1984-10-16	1991-07-24	Oligonucleotides with selectably cleavable and/or abasic sites

Publications (1)

Publication Number	Publication Date
CA2088257A1 true CA2088257A1 (en)	1992-01-28

Family

ID=27072214

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CA 2088257 Abandoned CA2088257A1 (en)	1990-07-27	1991-07-25	Incorporation of selectably clevable and/or abasic sites into oligonucleotide chains and reagents therefor

Country Status (4)

Country	Link
CA (1)	CA2088257A1 (pl)
IE (1)	IE912661A1 (pl)
PL (1)	PL170146B1 (pl)
PT (1)	PT98488B (pl)

1991
- 1991-07-25 PL PL91298545A patent/PL170146B1/pl unknown
- 1991-07-25 CA CA 2088257 patent/CA2088257A1/en not_active Abandoned
- 1991-07-29 IE IE266191A patent/IE912661A1/en unknown
- 1991-07-29 PT PT9848891A patent/PT98488B/pt not_active IP Right Cessation

Also Published As

Publication number	Publication date
IE912661A1 (en)	1992-01-29
PL170146B1 (pl)	1996-10-31
PT98488A (pt)	1992-05-29
PT98488B (pt)	1998-04-30

Legal Events

Date	Code	Title	Description
1997-08-07	EEER	Examination request
2000-07-25	FZDE	Dead

Publication	Publication Date	Title
US5367066A (en)	1994-11-22	Oligonucleotides with selectably cleavable and/or abasic sites
US5545730A (en)	1996-08-13	Multifunctional nucleic acid monomer
US5258506A (en)	1993-11-02	Photolabile reagents for incorporation into oligonucleotide chains
EP0777674B1 (en)	2001-11-28	Polynucleotide reagents having nonnucleotidic moieties, and associated methods of synthesis and use
JP2787775B2 (ja)	1998-08-20	固体支持体上にアンモニアに不安定な基で標識化されたオリゴヌクレオチド類の合成方法
JP2519406B2 (ja)	1996-07-31	保護剤を結合したオリゴヌクレオチド連鎖を含む新規化合物、及び該新規化合物の製造方法
JPH07233188A (ja)	1995-09-05	新規ヌクレオシドホスホルアミダイトおよびその製造方法
FR2607507A1 (fr)	1988-06-03	Nouveaux derives a-d-oligonucleotides, leur preparation et leur emploi
EP0688784A2 (de)	1995-12-27	Neue 3'-Modifizierte Oligonucleotid-Derivate
EP3898841A1 (en)	2021-10-27	Asymmetric rhodamine dye and use thereof in biological assays
US5252760A (en)	1993-10-12	Method of using colored phosphorylating reagents
FR2627590A1 (fr)	1989-08-25	Sonde d'acides nucleiques comportant un nucleotide terminal modifie chimiquement en 5(prime) (oh) en vue de son marquage non radioactif et procedes de preparation
CA2088257A1 (en)	1992-01-28	Incorporation of selectably clevable and/or abasic sites into oligonucleotide chains and reagents therefor
US5332845A (en)	1994-07-26	Phosphorylating reagents
JP3061659B2 (ja)	2000-07-10	２−｛２−（モノメトキシトリチルオキシ）エチルチオ｝エチル基および該基の使用法
JPH1129591A (ja)	1999-02-02	人工ヌクレアーゼ機能を有するオリゴデオキシリボヌクレオチド、ならびに、その製造用の中間体、その製造方法および使用方法
HK1000191A1 (en)	1998-01-27	Modified oligodeoxyribonucleotides, their preparation and their therapeutic use
HK1000191B (en)	1998-01-27	Modified oligodeoxyribonucleotides, their preparation and their therapeutic use