CA2108134A1 - Nucleases specifiques universelles - Google Patents

Nucleases specifiques universelles

Info

Publication number
CA2108134A1
CA2108134A1 CA002108134A CA2108134A CA2108134A1 CA 2108134 A1 CA2108134 A1 CA 2108134A1 CA 002108134 A CA002108134 A CA 002108134A CA 2108134 A CA2108134 A CA 2108134A CA 2108134 A1 CA2108134 A1 CA 2108134A1
Authority
CA
Canada
Prior art keywords
receptor
fusion protein
nucleotide sequence
dna
ligand
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002108134A
Other languages
English (en)
Inventor
Yie-Hwa Chang
John A. Smith
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
General Hospital Corp
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2108134A1 publication Critical patent/CA2108134A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/22—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a Strep-tag
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C12N2310/3513—Protein; Peptide

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA002108134A 1991-06-11 1992-05-06 Nucleases specifiques universelles Abandoned CA2108134A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US71290591A 1991-06-11 1991-06-11
US07/712,905 1991-06-11

Publications (1)

Publication Number Publication Date
CA2108134A1 true CA2108134A1 (fr) 1992-12-12

Family

ID=24864017

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002108134A Abandoned CA2108134A1 (fr) 1991-06-11 1992-05-06 Nucleases specifiques universelles

Country Status (7)

Country Link
EP (1) EP0589958A4 (fr)
JP (1) JPH06510901A (fr)
AU (1) AU1972392A (fr)
CA (1) CA2108134A1 (fr)
IL (1) IL101837A0 (fr)
NZ (1) NZ242694A (fr)
WO (1) WO1992022642A1 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5436150A (en) * 1992-04-03 1995-07-25 The Johns Hopkins University Functional domains in flavobacterium okeanokoities (foki) restriction endonuclease
EP1340812B1 (fr) * 1993-02-12 2011-06-15 The Johns-Hopkins University Domaines fonctionnelles dans l'endonucléase de restriction d'okéanokoites flavobactérium (FOKI)
CN111751540B (zh) * 2020-07-20 2023-03-03 烟台大学 一种用于核酸酶检测的sers横向流动试纸条及检测方法

Also Published As

Publication number Publication date
JPH06510901A (ja) 1994-12-08
EP0589958A1 (fr) 1994-04-06
AU1972392A (en) 1993-01-12
WO1992022642A1 (fr) 1992-12-23
NZ242694A (en) 1993-07-27
IL101837A0 (en) 1992-12-30
EP0589958A4 (fr) 1995-04-26

Similar Documents

Publication Publication Date Title
JP3370094B2 (ja) リーダー配列なしに細胞質外に輸送される融合ポリペプチドの発現
Bragg et al. Nucleotide sequence and analysis of the lethal factor gene (lef) from Bacillus anthracis
US5863765A (en) Production in yeasts of stable antibody fragments
McCarter et al. Substrate specificity of the Escherichia coli outer membrane protease OmpT
Liu et al. The yeast KEM1 gene encodes a nuclease specific for G4 tetraplex DNA: implication of in vivo functions for this novel DNA structure
Fortkamp et al. Cloning and expression in Escherichia coli of a synthetic DNA for hirudin, the blood coagulation inhibitor in the leech
JP4493271B2 (ja) Ｃ型レクチン様ドメインの骨格構造を有する蛋白質のコンビナトリアルライブラリ
US6265196B1 (en) Methods for inactivating target DNA and for detecting conformational change in a nucleic acid
JPH05503000A (ja) 新規の遺伝子及びポリペチドの無細胞合成並びに単離
EP0230222A1 (fr) Expression du gène gag de HTLV-III
US6027893A (en) Method of identifying a nucleic acid using triple helix formation of adjacently annealed probes
JP3644961B2 (ja) ストレプトコッカス・ピオゲネスから誘導された組換えｄｎアーゼｂ
Schaefer et al. Ribosome-binding sites and RNA-processing sites in the transcript of the Escherichia coli unc operon
Parker et al. Adenovirus DNA polymerase: domain organisation and interaction with preterminal protein
Hines et al. The Crithidia fasciculata KAP1 gene encodes a highly basic protein associated with kinetoplast DNA
Li et al. Functional analysis of affinity-purified polyhistidine-tagged DnaA protein
Trojanowska et al. The bacteriophage T4 regA gene: primary sequence of a translational repressor
CA2108134A1 (fr) Nucleases specifiques universelles
Cyrne et al. The Tetrahymena chaperonin subunit CCTη gene is coexpressed with CCTγ gene during cilia biogenesis and cell sexual reproduction
Parkinson et al. Catalytic and binding mutants of the junction-resolving enzyme endonuclease I of bacteriophage T7: role of acidic residues
JPH11510683A (ja) エピトープタギングシステム
EP0329710A1 (fr) Production microbienne d'oligomeres peptidiques
Birkenbihl et al. Endonuclease VII has two DNA‐binding sites each composed from one N‐and one C‐terminus provided by different subunits of the protein dimer
Liu et al. A protein (M9) associated with monoclonal antibody-mediated agglutination of Mycoplasma gallisepticum is a member of the pMGA family
Ku et al. Phage display of catalytically active staphylococcal nuclease

Legal Events

Date Code Title Description
FZDE Discontinued