CA2151731A1 - Sonde marquee par une lipase - Google Patents

Sonde marquee par une lipase

Info

Publication number
CA2151731A1
CA2151731A1 CA 2151731 CA2151731A CA2151731A1 CA 2151731 A1 CA2151731 A1 CA 2151731A1 CA 2151731 CA2151731 CA 2151731 CA 2151731 A CA2151731 A CA 2151731A CA 2151731 A1 CA2151731 A1 CA 2151731A1
Authority
CA
Canada
Prior art keywords
lipase
enzyme
activity
solution
alkaline phosphatase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA 2151731
Other languages
English (en)
Inventor
Fritz Pittner
Thomas Schalkhammer
Bernhard Ecker
Eva Kynclova
Werner Wakolbinger
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2151731A1 publication Critical patent/CA2151731A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/535—Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Afin d'améliorer la stabilité thermique et chimique d'une sonde marquée par une enzyme, on utilise comme enzyme de la lipase, de préférence extraite de Candida rugosa, et dont les isoenzymes ou analogues structuraux sont homologues d'acides aminés pour au moins 70 % et ont une activité de lipase.
CA 2151731 1993-10-15 1994-10-13 Sonde marquee par une lipase Abandoned CA2151731A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ATA207193 1993-10-15
AT207193A AT400036B (de) 1993-10-15 1993-10-15 Mit enzym markierte probe oder testsubstanz

Publications (1)

Publication Number Publication Date
CA2151731A1 true CA2151731A1 (fr) 1995-04-20

Family

ID=3527156

Family Applications (1)

Application Number Title Priority Date Filing Date
CA 2151731 Abandoned CA2151731A1 (fr) 1993-10-15 1994-10-13 Sonde marquee par une lipase

Country Status (6)

Country Link
EP (1) EP0679257A1 (fr)
JP (1) JPH07509618A (fr)
AT (1) AT400036B (fr)
AU (1) AU671392B2 (fr)
CA (1) CA2151731A1 (fr)
WO (1) WO1995010775A1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050074828A1 (en) * 2003-07-16 2005-04-07 Dimagno Theodore John Uae of lipase for high-density lipoprotein cholesterol detection

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK601984D0 (da) * 1984-12-14 1984-12-14 Hansens Lab Fremgangsmaade til fremstilling af genprodukter
EP0350808B1 (fr) * 1988-07-11 1995-11-08 Roche Diagnostics GmbH Méthode pour la détection de substances avec activité hydrolase
US5093256A (en) * 1989-02-22 1992-03-03 Shen Gwo Jenn Essentially purified, thermostable and alkalophilic lipase from bacillus sp. a30-1 atcc 53841
US5108916A (en) * 1989-06-05 1992-04-28 Rhone-Poulenc Rorer, S.A. Process for stereoselectively hydrolyzing, transesterifying or esterifying with immobilized isozyme of lipase from candida rugosa
US5248618A (en) * 1991-06-05 1993-09-28 Life Technologies, Inc. Methods for generating light with chemiluminescent dioxetanes activated by anchimeric assisted cleavage

Also Published As

Publication number Publication date
WO1995010775A1 (fr) 1995-04-20
JPH07509618A (ja) 1995-10-26
AT400036B (de) 1995-09-25
AU671392B2 (en) 1996-08-22
EP0679257A1 (fr) 1995-11-02
AU7855794A (en) 1995-05-04
ATA207193A (de) 1995-01-15

Similar Documents

Publication Publication Date Title
EP0219286B1 (fr) Composés ioniques contenant la mériquinone cationique d'une benzidine, trousses les contenant et leur utilisation pour l'examen
US4556643A (en) Assay method and probe for polynucleotide sequences
US6610504B1 (en) Methods of determining SAM-dependent methyltransferase activity using a mutant SAH hydrolase
US6326136B1 (en) Macromolecular conjugate made using unsaturated aldehydes
US20030219848A1 (en) Short enzyme donor fragment
JPH06178700A (ja) 相補的核酸配列の検出方法及びそのためのキット
US6362328B1 (en) Assays and probes with enzyme labels
EP0123300A2 (fr) Complexes de composés biologiquement actifs ou fonctionnels et méthode pour leur préparation et leur utilisation
AU671392B2 (en) Lipase-labelled probe
Zauner et al. Tethered DNA hairpins facilitate electrochemical detection of DNA ligation
JPS63282659A (ja) 核酸の測定方法および測定剤
Lima et al. Immobilization of commercial acid phosphatases from wheat germ and potato onto ion exchangers
EP4148144B1 (fr) Composition pour identifier le dopage génétique de l'érythropoïétine et son utilisation
JPH06507318A (ja) 染色体構造および転位の検出方法
US12037631B2 (en) System and method for detecting a target enzyme
JP4035738B2 (ja) 新規なアルカリホスファターゼ
EP0739421B1 (fr) Dosage par hybridation
Meiss et al. Microtiter-plate assay and related assays for nonspecific endonucleases
US5955369A (en) Method for the determination of mutant restriction enzymes
AU642854B2 (en) Macromolecular conjugate
US20050239078A1 (en) Sequence tag microarray and method for detection of multiple proteins through DNA methods
JP3658820B2 (ja) 試料中のリガンド検出法およびそのための試薬
Tannous et al. Heterobifunctional linker between antibodies and reporter genes for immunoassay development
Hermsdorf Rapid assay to detect peptidases in column effluent fractions using l-amino acid oxidase

Legal Events

Date Code Title Description
EEER Examination request
FZDE Dead