CA2175202C - Procede de stabilisation de la proteine c ou de la proteine c activee et composition stabilisante obtenue par ce procede - Google Patents

Procede de stabilisation de la proteine c ou de la proteine c activee et composition stabilisante obtenue par ce procede Download PDF

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Publication number
CA2175202C
CA2175202C CA002175202A CA2175202A CA2175202C CA 2175202 C CA2175202 C CA 2175202C CA 002175202 A CA002175202 A CA 002175202A CA 2175202 A CA2175202 A CA 2175202A CA 2175202 C CA2175202 C CA 2175202C
Authority
CA
Canada
Prior art keywords
protein
activated protein
activated
activity
apc
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA002175202A
Other languages
English (en)
Other versions
CA2175202A1 (fr
Inventor
Kazumasa Miyata
Yoshinori Akimoto
Yoichi Ogata
Tomohiro Nakagaki
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Chemo Sero Therapeutic Research Institute Kaketsuken
Teijin Ltd
Original Assignee
Chemo Sero Therapeutic Research Institute Kaketsuken
Teijin Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP5292500A external-priority patent/JP2886061B2/ja
Application filed by Chemo Sero Therapeutic Research Institute Kaketsuken, Teijin Ltd filed Critical Chemo Sero Therapeutic Research Institute Kaketsuken
Publication of CA2175202A1 publication Critical patent/CA2175202A1/fr
Application granted granted Critical
Publication of CA2175202C publication Critical patent/CA2175202C/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6464—Protein C (3.4.21.69)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46—Hydrolases (3)
    • A61K38/48—Hydrolases (3) acting on peptide bonds (3.4)
    • A61K38/482—Serine endopeptidases (3.4.21)
    • A61K38/4866—Protein C (3.4.21.69)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Medicinal Preparation (AREA)

Abstract

La présente invention concerne un procédé visant à la stabilisation de la protéine C activée ou non activée pour les opérations de séparation/purification par conductivité, de lyophilisation, de traitement thermique ou de stockage. La composition stabilisée est obtenue par mélange d'un tampon salin contenant la protéine C activée ou non et des ions sodium avec au moins un acide aminé et de l'albumine et/ou un surfactant non ionique.
CA002175202A 1993-10-29 1994-10-27 Procede de stabilisation de la proteine c ou de la proteine c activee et composition stabilisante obtenue par ce procede Expired - Lifetime CA2175202C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP5292500A JP2886061B2 (ja) 1993-10-29 1993-10-29 プロテインｃもしくは活性化プロテインｃの安定化方法及び安定化組成物
JP292500/1993 1993-10-29
PCT/JP1994/001804 WO1995011698A1 (fr) 1993-10-29 1994-10-27 PROCEDE DE STABILISATION DE PROTéINE C ACTIVEE OU NON ACTIVEE ET COMPOSITION STABILISEE

Publications (2)

Publication Number Publication Date
CA2175202A1 CA2175202A1 (fr) 1995-05-04
CA2175202C true CA2175202C (fr) 2006-09-19

Family

ID=37056965

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002175202A Expired - Lifetime CA2175202C (fr) 1993-10-29 1994-10-27 Procede de stabilisation de la proteine c ou de la proteine c activee et composition stabilisante obtenue par ce procede

Country Status (1)

Country Link
CA (1) CA2175202C (fr)

Also Published As

Publication number Publication date
CA2175202A1 (fr) 1995-05-04

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Effective date: 20141027