CA2189486A1 - Analyse de l'activite enzymatique utilisant des substrats immobilises, marques par fluorescence - Google Patents

Analyse de l'activite enzymatique utilisant des substrats immobilises, marques par fluorescence

Info

Publication number
CA2189486A1
CA2189486A1 CA 2189486 CA2189486A CA2189486A1 CA 2189486 A1 CA2189486 A1 CA 2189486A1 CA 2189486 CA2189486 CA 2189486 CA 2189486 A CA2189486 A CA 2189486A CA 2189486 A1 CA2189486 A1 CA 2189486A1
Authority
CA
Canada
Prior art keywords
microspheres
activity
gelatinase
substrate
fitc
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA 2189486
Other languages
English (en)
Inventor
Yves St-Pierre
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to CA 2189486 priority Critical patent/CA2189486A1/fr
Priority to AU48595/97A priority patent/AU4859597A/en
Priority to PCT/CA1997/000823 priority patent/WO1998020153A1/fr
Priority to CA 2220656 priority patent/CA2220656A1/fr
Priority to EP97911088A priority patent/EP0951562A1/fr
Publication of CA2189486A1 publication Critical patent/CA2189486A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/78Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin, cold insoluble globulin [CIG]
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90Enzymes; Proenzymes
    • G01N2333/914Hydrolases (3)
    • G01N2333/948Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/95Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
    • G01N2333/964Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue
    • G01N2333/96425Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals
    • G01N2333/96427Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general
    • G01N2333/9643Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general with EC number
    • G01N2333/96486Metalloendopeptidases (3.4.24)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA 2189486 1996-11-04 1996-11-04 Analyse de l'activite enzymatique utilisant des substrats immobilises, marques par fluorescence Abandoned CA2189486A1 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
CA 2189486 CA2189486A1 (fr) 1996-11-04 1996-11-04 Analyse de l'activite enzymatique utilisant des substrats immobilises, marques par fluorescence
AU48595/97A AU4859597A (en) 1996-11-04 1997-11-04 Fluorescence-labeled substrates and their use to analyze enzyme activity using flow cytometry
PCT/CA1997/000823 WO1998020153A1 (fr) 1996-11-04 1997-11-04 Substrats a marquage fluorescent et leur utilisation pour analyser une activite enzymatique par cytometrie de flux
CA 2220656 CA2220656A1 (fr) 1996-11-04 1997-11-04 Substrats marques par fluorescence et leur emploi pour analyser l'activite enzymatique par cytometrie de flux
EP97911088A EP0951562A1 (fr) 1996-11-04 1997-11-04 Substrats a marquage fluorescent et leur utilisation pour analyser une activite enzymatique par cytometrie de flux

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CA 2189486 CA2189486A1 (fr) 1996-11-04 1996-11-04 Analyse de l'activite enzymatique utilisant des substrats immobilises, marques par fluorescence

Publications (1)

Publication Number Publication Date
CA2189486A1 true CA2189486A1 (fr) 1998-05-04

Family

ID=4159198

Family Applications (1)

Application Number Title Priority Date Filing Date
CA 2189486 Abandoned CA2189486A1 (fr) 1996-11-04 1996-11-04 Analyse de l'activite enzymatique utilisant des substrats immobilises, marques par fluorescence

Country Status (4)

Country Link
EP (1) EP0951562A1 (fr)
AU (1) AU4859597A (fr)
CA (1) CA2189486A1 (fr)
WO (1) WO1998020153A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111742209A (zh) * 2018-12-21 2020-10-02 索尼公司 粒子确认方法、粒子捕获芯片、以及粒子分析系统

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2255599C (fr) 1996-04-25 2006-09-05 Bioarray Solutions, Llc Assemblage electrocinetique de particules proches des surfaces regule par la lumiere
US9709559B2 (en) 2000-06-21 2017-07-18 Bioarray Solutions, Ltd. Multianalyte molecular analysis using application-specific random particle arrays
US7262063B2 (en) 2001-06-21 2007-08-28 Bio Array Solutions, Ltd. Directed assembly of functional heterostructures
CA2497740C (fr) 2001-10-15 2011-06-21 Bioarray Solutions, Ltd. Analyse multiplexee de loci polymorphes par une methode de detection basee sur le prolongement de la sonde
US7041453B2 (en) 2002-08-22 2006-05-09 Bioarray Solutions Ltd. Molecular constructs and methods of use for detection of biochemical reactions
AU2003298655A1 (en) 2002-11-15 2004-06-15 Bioarray Solutions, Ltd. Analysis, secure access to, and transmission of array images
CA2539824C (fr) 2003-09-22 2015-02-03 Xinwen Wang Polyelectrolyte immobilise en surface a plusieurs groupes fonctionnels pouvant se lier de maniere covalente a des biomolecules
CA2544041C (fr) 2003-10-28 2015-12-08 Bioarray Solutions Ltd. Optimisation de l'analyse de l'expression genique a l'aide de sondes de capture immobilisees
US7848889B2 (en) 2004-08-02 2010-12-07 Bioarray Solutions, Ltd. Automated analysis of multiplexed probe-target interaction patterns: pattern matching and allele identification
EP2315842A2 (fr) * 2008-07-28 2011-05-04 Dorit Arad Méthodes et compositions destinées à la détection d'un agent pathogène, d'une maladie, d'un état pathologique, ou d'un biomarqueur se rapportant à ceux-ci
KR102587439B1 (ko) * 2017-06-27 2023-10-10 코리올리스 파마 리서치 게엠베하 폴리소르베이트 정량 어세이

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1339812C (fr) * 1988-02-19 1998-04-14 Tamami Koyama Substance de remplissage utilisee pour mesurer l'activite enzymatique, colonne remplie avec cette substance et methode pour l'activite enzymatique au moyen de cette colonne
NO164622C (no) * 1988-05-11 1990-10-24 Tore Lindmo Binaer immunometrisk partikkelbasert metode for maaling av spesifikke serum-antigener ved hjelp av vaeskestroemsmikrofotometri og et ferdigpreparert maaloppsett derav.
US5416003A (en) * 1993-04-14 1995-05-16 Litmus Concepts, Inc. Reporter enzyme release technology: methods of assaying for the presence of aspartic proteases and other hydrolytic enzyme activities

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111742209A (zh) * 2018-12-21 2020-10-02 索尼公司 粒子确认方法、粒子捕获芯片、以及粒子分析系统
US12313639B2 (en) 2018-12-21 2025-05-27 Sony Corporation Particle confirming method, particle trapping chip, and particle analyzing system

Also Published As

Publication number Publication date
AU4859597A (en) 1998-05-29
EP0951562A1 (fr) 1999-10-27
WO1998020153A1 (fr) 1998-05-14

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Legal Events

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FZDE Dead