CA2205657A1 - Sequences de stearoyl-(proteine vectrice d'acyle)-thioesterase vegetales et procedes pour augmenter la teneur en stearate des huiles de graines vegetales - Google Patents

Sequences de stearoyl-(proteine vectrice d'acyle)-thioesterase vegetales et procedes pour augmenter la teneur en stearate des huiles de graines vegetales

Info

Publication number
CA2205657A1
CA2205657A1 CA 2205657 CA2205657A CA2205657A1 CA 2205657 A1 CA2205657 A1 CA 2205657A1 CA 2205657 CA2205657 CA 2205657 CA 2205657 A CA2205657 A CA 2205657A CA 2205657 A1 CA2205657 A1 CA 2205657A1
Authority
CA
Canada
Prior art keywords
plant
acp
seed
stearoyl
stearate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA 2205657
Other languages
English (en)
Inventor
Jean Kridl
Vic C. Knauf
Michael W. Lassner
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Monsanto Co
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2205657A1 publication Critical patent/CA2205657A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Fats And Perfumes (AREA)

Abstract

L'invention concerne les thioestérases végétales, en particulier des acyl-PVA thioestérases ayant une activité significative sur la stéaroyl-PVA. On décrit des constructions d'ADN utiles pour l'expression d'une stéaroyl-PVA thioestérase végétale dans des graines de plante. Ces constructions vont contenir une séquence d'ADN codant pour la stéaroyl-PVA thioestérase végétale choisie sous le contrôle d'éléments régulateurs capables de diriger préférentiellement l'expression de la stéaroyl-PVA thioestérase végétale vers des tissus de graines, plutôt que d'autres tissus de plante, quand une telle construction est exprimée dans une plante transgénique. Cette invention concerne également des procédés d'utilisation d'une séquence d'ADN codant une stéaroyl-PVA thioestérase de plante pour la modification d'un stéarate produit dans une cellule de graine de plante. Une thioestérase de plante ayant une activité significative sur la stéaroyl-PVA donnée ici comme exemple vient de mangoustan. La concentration modifiée de stéarate peut être obtenue par l'expression de la thioestérase de mangoustan seule ou en combinaison avec des constructions antisense diminuant la stéaroyl-PVA désaturase dans les graines de plantes.
CA 2205657 1995-09-29 1996-09-30 Sequences de stearoyl-(proteine vectrice d'acyle)-thioesterase vegetales et procedes pour augmenter la teneur en stearate des huiles de graines vegetales Abandoned CA2205657A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US53708395A 1995-09-29 1995-09-29
US08/537,083 1995-09-29

Publications (1)

Publication Number Publication Date
CA2205657A1 true CA2205657A1 (fr) 1997-04-03

Family

ID=24141133

Family Applications (1)

Application Number Title Priority Date Filing Date
CA 2205657 Abandoned CA2205657A1 (fr) 1995-09-29 1996-09-30 Sequences de stearoyl-(proteine vectrice d'acyle)-thioesterase vegetales et procedes pour augmenter la teneur en stearate des huiles de graines vegetales

Country Status (4)

Country Link
EP (1) EP0795020A4 (fr)
JP (1) JPH10510438A (fr)
CA (1) CA2205657A1 (fr)
WO (1) WO1997012047A1 (fr)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6150512A (en) * 1995-05-15 2000-11-21 Yuan; Ling Engineering plant thioesterases and disclosure of plant thioesterases having novel substrate specificity
US6331664B1 (en) 1997-05-05 2001-12-18 Dow Agrosciences Llc Acyl-ACP thioesterase nucleic acids from maize and methods of altering palmitic acid levels in transgenic plants therewith
EP1124973B1 (fr) * 1998-07-21 2008-02-13 Dow AgroSciences LLC Regulation negative de proteines vegetales a mediation par anticorps
US6365802B2 (en) * 1998-08-14 2002-04-02 Calgene Llc Methods for increasing stearate content in soybean oil
US6713117B1 (en) 1998-10-02 2004-03-30 Dharma Kodali Vegetable oil having elevated stearic acid content
US6388113B1 (en) 1999-06-04 2002-05-14 Consejo Superior De Investigaciones Cientificas ( Csic) High oleic/high stearic sunflower oils
EP1185160B1 (fr) 1999-06-04 2006-08-23 Consejo Superior de Investigaciones Cientificas Plantes, graines et huiles de tournesol a teneur elevee en acide stearique et en acide oleique
US7592015B2 (en) 1999-06-04 2009-09-22 Consejo Superior De Investigaciones Cientificas Use of high oleic high stearic oils
US6348610B1 (en) 1999-06-04 2002-02-19 Consejo Superior De Investigaciones Cientificas (Csic) Oil from seeds with a modified fatty acid composition
CA2700545C (fr) * 2005-06-30 2012-08-28 Toyota Jidosha Kabushiki Kaisha Gene innovant implique dans la biosynthese de l'acide petroselinique et procede de production d'acide petroselinique
CN105647842A (zh) 2008-12-23 2016-06-08 Reg生命科学有限责任公司 硫酯酶相关的方法和组合物

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5512482A (en) * 1990-04-26 1996-04-30 Calgene, Inc. Plant thioesterases
US5344771A (en) * 1990-04-26 1994-09-06 Calgene, Inc. Plant thiosterases
EP0563191B2 (fr) * 1990-12-20 2000-01-19 E.I. Du Pont De Nemours And Company Sequences de nucleotides de genes d'acyle-acp thioesterase de soja
US5455167A (en) * 1991-05-21 1995-10-03 Calgene Inc. Medium-chain thioesterases in plants
GB9204583D0 (en) * 1992-03-03 1992-04-15 Unilever Plc Recombinant plant enzyme
EP0728212A1 (fr) * 1993-11-10 1996-08-28 Calgene, Inc. Sequences de thioesterases vegetales actives sur les substrats acyl-(proteine vectrice d'acyle)
JPH11505115A (ja) * 1995-05-15 1999-05-18 カルジーン，インコーポレーテッド 植物チオエステラーゼの遺伝子操作および新規な基質特異性を有する植物チオエステラーゼの開示

Also Published As

Publication number Publication date
EP0795020A1 (fr) 1997-09-17
EP0795020A4 (fr) 1999-02-24
WO1997012047A1 (fr) 1997-04-03
JPH10510438A (ja) 1998-10-13

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Legal Events

Date Code Title Description
FZDE Dead