CA2210986A1 - Procede visant a ameliorer l'efficacite du criblage de cellules fusionnees - Google Patents
Procede visant a ameliorer l'efficacite du criblage de cellules fusionneesInfo
- Publication number
- CA2210986A1 CA2210986A1 CA002210986A CA2210986A CA2210986A1 CA 2210986 A1 CA2210986 A1 CA 2210986A1 CA 002210986 A CA002210986 A CA 002210986A CA 2210986 A CA2210986 A CA 2210986A CA 2210986 A1 CA2210986 A1 CA 2210986A1
- Authority
- CA
- Canada
- Prior art keywords
- cells
- fusion
- cell line
- yac
- marker
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
- C12N5/12—Fused cells, e.g. hybridomas
- C12N5/16—Animal cells
- C12N5/163—Animal cells one of the fusion partners being a B or a T lymphocyte
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
- C12N5/12—Fused cells, e.g. hybridomas
- C12N5/16—Animal cells
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Abstract
On divulgue une méthode pour améliorer l'efficacité du criblage des cellules de fusion pour déceler la présence de gènes recherchés. La méthode consiste à doter le gène recherché d'un marqueur pour surmonter la sensibilité d'un partenaire de fusion cellulaire immortalisant à certaines conditions du milieu. La méthode est illustrée par l'inclusion d'un marqueur dans les gènes d'immunoglobuline présents dans les lymphocytes B.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US37627995A | 1995-01-20 | 1995-01-20 | |
| US376,279 | 1995-01-20 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2210986A1 true CA2210986A1 (fr) | 1996-07-25 |
Family
ID=23484361
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002210986A Abandoned CA2210986A1 (fr) | 1995-01-20 | 1996-01-19 | Procede visant a ameliorer l'efficacite du criblage de cellules fusionnees |
Country Status (6)
| Country | Link |
|---|---|
| EP (1) | EP0804604A2 (fr) |
| JP (1) | JPH10512449A (fr) |
| KR (1) | KR19980701536A (fr) |
| AU (1) | AU4906696A (fr) |
| CA (1) | CA2210986A1 (fr) |
| WO (1) | WO1996022380A2 (fr) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6682736B1 (en) | 1998-12-23 | 2004-01-27 | Abgenix, Inc. | Human monoclonal antibodies to CTLA-4 |
| US7109003B2 (en) | 1998-12-23 | 2006-09-19 | Abgenix, Inc. | Methods for expressing and recovering human monoclonal antibodies to CTLA-4 |
| NZ517202A (en) | 1999-08-24 | 2004-05-28 | Medarex Inc | Human CTLA-4 antibodies and their uses |
| US7605238B2 (en) | 1999-08-24 | 2009-10-20 | Medarex, Inc. | Human CTLA-4 antibodies and their uses |
| ES2645563T3 (es) | 2001-11-30 | 2017-12-05 | Amgen Fremont Inc. | Animales transgénicos que portan genes de cadena ligera de Ig humana |
| NZ536420A (en) | 2002-04-12 | 2008-04-30 | Medarex Inc | Methods of treatment using CTLA-4 antibodies |
| NZ568016A (en) | 2005-12-07 | 2011-12-22 | Medarex Inc | CTLA-4 antibody dosage escalation regimens |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2637613B1 (fr) * | 1987-06-19 | 1991-09-27 | Transgene Sa | Procede de preparation de lignees cellulaires stables pour la production de proteines determinees, a partir d'animaux transgeniques; lignees cellulaires tumorales et proteines obtenues |
| JP3008208B2 (ja) * | 1990-06-01 | 2000-02-14 | 武田薬品工業株式会社 | 新規ハイブリドーマ，その製造法および生理活性物質の製造法 |
| ES2301158T3 (es) * | 1992-07-24 | 2008-06-16 | Amgen Fremont Inc. | Produccion de anticuerpos xenogenicos. |
-
1996
- 1996-01-19 EP EP96905259A patent/EP0804604A2/fr not_active Withdrawn
- 1996-01-19 WO PCT/US1996/001169 patent/WO1996022380A2/fr not_active Ceased
- 1996-01-19 AU AU49066/96A patent/AU4906696A/en not_active Abandoned
- 1996-01-19 JP JP8522458A patent/JPH10512449A/ja active Pending
- 1996-01-19 CA CA002210986A patent/CA2210986A1/fr not_active Abandoned
- 1996-01-19 KR KR1019970704927A patent/KR19980701536A/ko not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| JPH10512449A (ja) | 1998-12-02 |
| KR19980701536A (ko) | 1998-05-15 |
| AU4906696A (en) | 1996-08-07 |
| WO1996022380A3 (fr) | 1996-09-12 |
| WO1996022380A2 (fr) | 1996-07-25 |
| EP0804604A2 (fr) | 1997-11-05 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FZDE | Discontinued | ||
| FZDE | Discontinued |
Effective date: 20020121 |