CA2236616C - Detection d'acides nucleique par extinction de la fluorescence - Google Patents
Detection d'acides nucleique par extinction de la fluorescence Download PDFInfo
- Publication number
- CA2236616C CA2236616C CA002236616A CA2236616A CA2236616C CA 2236616 C CA2236616 C CA 2236616C CA 002236616 A CA002236616 A CA 002236616A CA 2236616 A CA2236616 A CA 2236616A CA 2236616 C CA2236616 C CA 2236616C
- Authority
- CA
- Canada
- Prior art keywords
- primer
- fluorescence
- donor fluorophore
- amplification
- target
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/855,085 | 1997-05-13 | ||
| US08/855,085 US5846726A (en) | 1997-05-13 | 1997-05-13 | Detection of nucleic acids by fluorescence quenching |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2236616A1 CA2236616A1 (fr) | 1998-11-13 |
| CA2236616C true CA2236616C (fr) | 2002-04-23 |
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| Application Number | Title | Priority Date | Filing Date |
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| CA002236616A Expired - Lifetime CA2236616C (fr) | 1997-05-13 | 1998-05-04 | Detection d'acides nucleique par extinction de la fluorescence |
Country Status (5)
| Country | Link |
|---|---|
| US (3) | US5846726A (fr) |
| EP (1) | EP0878554B2 (fr) |
| JP (1) | JP3477366B2 (fr) |
| CA (1) | CA2236616C (fr) |
| DE (1) | DE69837125T3 (fr) |
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| JPS613063A (ja) * | 1984-06-18 | 1986-01-09 | Fujirebio Inc | 発光物質を利用したポリヌクレオチドの測定方法 |
| US5118605A (en) * | 1984-10-16 | 1992-06-02 | Chiron Corporation | Polynucleotide determination with selectable cleavage sites |
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| US5538848A (en) * | 1994-11-16 | 1996-07-23 | Applied Biosystems Division, Perkin-Elmer Corp. | Method for detecting nucleic acid amplification using self-quenching fluorescence probe |
| US5547861A (en) * | 1994-04-18 | 1996-08-20 | Becton, Dickinson And Company | Detection of nucleic acid amplification |
| CA2209080C (fr) * | 1994-12-30 | 2008-07-29 | Georgetown University | Methode de detection fluorometrique de clivage d'acide nucleique |
| US5550025A (en) * | 1995-07-19 | 1996-08-27 | Becton, Dickinson And Company | Detection of hydrophobic amplification products by extraction into an organic phase |
| US5846726A (en) * | 1997-05-13 | 1998-12-08 | Becton, Dickinson And Company | Detection of nucleic acids by fluorescence quenching |
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- 1998-05-11 DE DE69837125.9T patent/DE69837125T3/de not_active Expired - Lifetime
- 1998-05-11 JP JP12706898A patent/JP3477366B2/ja not_active Expired - Lifetime
- 1998-05-11 EP EP98108491.6A patent/EP0878554B2/fr not_active Expired - Lifetime
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| EP0878554B2 (fr) | 2017-03-22 |
| DE69837125T3 (de) | 2017-08-31 |
| JP3477366B2 (ja) | 2003-12-10 |
| JPH11123083A (ja) | 1999-05-11 |
| CA2236616A1 (fr) | 1998-11-13 |
| US6054279A (en) | 2000-04-25 |
| EP0878554A3 (fr) | 2001-11-28 |
| DE69837125T2 (de) | 2007-12-13 |
| US5919630A (en) | 1999-07-06 |
| EP0878554B1 (fr) | 2007-02-21 |
| EP0878554A2 (fr) | 1998-11-18 |
| US5846726A (en) | 1998-12-08 |
| DE69837125D1 (de) | 2007-04-05 |
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