CA2247131A1 - Procedes modifies d'expansion rapide (rem modifie) permettant la propagation in vitro de lymphocytes t - Google Patents
Procedes modifies d'expansion rapide (rem modifie) permettant la propagation in vitro de lymphocytes t Download PDFInfo
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- CA2247131A1 CA2247131A1 CA002247131A CA2247131A CA2247131A1 CA 2247131 A1 CA2247131 A1 CA 2247131A1 CA 002247131 A CA002247131 A CA 002247131A CA 2247131 A CA2247131 A CA 2247131A CA 2247131 A1 CA2247131 A1 CA 2247131A1
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
- C12N5/0636—T lymphocytes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/20—Cytokines; Chemokines
- C12N2501/23—Interleukins [IL]
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/50—Cell markers; Cell surface determinants
- C12N2501/515—CD3, T-cell receptor complex
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/50—Cell markers; Cell surface determinants
- C12N2501/58—Adhesion molecules, e.g. ICAM, VCAM, CD18 (ligand), CD11 (ligand), CD49 (ligand)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/50—Cell markers; Cell surface determinants
- C12N2501/599—Cell markers; Cell surface determinants with CD designations not provided for elsewhere
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/11—Coculture with; Conditioned medium produced by blood or immune system cells
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- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
L'invention porte sur un procédé modifié d'expansion rapide (dit PBMC-REM basse ou REM modifié), permettant de produire rapidement d'importantes quantités de lymphocytes T y compris des lymphocytes T cytolytiques et auxiliaires sans recourir à de forts excès de globules sanguins périphériques mononucléaires (PBMC) ou de cellules lymphoblastoïdes (LCL) à transformation EBV, caractéristiques de la PBMC-REM haute. Des expansions clonales supérieures à 500 fois peuvent être atteintes au cours d'un seul cycle de stimulation d'environ 8 à 14 jours.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US61071096A | 1996-03-04 | 1996-03-04 | |
| US08/610,710 | 1996-03-04 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2247131A1 true CA2247131A1 (fr) | 1997-09-12 |
Family
ID=24446115
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002247131A Pending CA2247131A1 (fr) | 1996-03-04 | 1997-03-03 | Procedes modifies d'expansion rapide (rem modifie) permettant la propagation in vitro de lymphocytes t |
Country Status (4)
| Country | Link |
|---|---|
| AU (1) | AU726198B2 (fr) |
| CA (1) | CA2247131A1 (fr) |
| IL (1) | IL125884A0 (fr) |
| WO (1) | WO1997032970A1 (fr) |
Families Citing this family (28)
| Publication number | Priority date | Publication date | Assignee | Title |
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| JP4389039B2 (ja) * | 1998-04-09 | 2009-12-24 | 株式会社リンフォテック | アロジェニックな活性化ｃｄ４陽性細胞を主成分とする医薬組成物、およびその製造方法、ならびに該医薬組成物調製用キット |
| US7541184B2 (en) | 2000-02-24 | 2009-06-02 | Invitrogen Corporation | Activation and expansion of cells |
| US7572631B2 (en) | 2000-02-24 | 2009-08-11 | Invitrogen Corporation | Activation and expansion of T cells |
| CN1222612C (zh) * | 2000-08-16 | 2005-10-12 | 宝生物工程株式会社 | 抗原特异细胞毒性t细胞的扩大培养方法 |
| US20040071671A1 (en) | 2001-02-20 | 2004-04-15 | Leturcq Didier J. | Cell therapy method for the treatment of tumors |
| US20030077248A1 (en) * | 2001-02-20 | 2003-04-24 | Ann Moriarty | Cell therapy method for the treatment of tumors |
| DE10120505A1 (de) * | 2001-04-26 | 2002-11-07 | Rudolf Wank | Verwendung von stimulierten mononukleären Zellen des peripheren Blutes zur Behandlung von Krebserkrankungen |
| JP4949607B2 (ja) * | 2001-08-15 | 2012-06-13 | タカラバイオ株式会社 | 抗原特異的細胞傷害性ｔ細胞拡大培養方法 |
| ES2397319T3 (es) | 2002-03-25 | 2013-03-06 | Takara Bio Inc. | Proceso para producir linfocitos citotóxicos |
| JP4562353B2 (ja) * | 2002-04-08 | 2010-10-13 | 株式会社リンフォテック | Ｈｌａ一致他人由来活性化リンパ球を含有してなる腫瘍・感染症および自己免疫疾患の予防・治療用製剤 |
| US20050084967A1 (en) | 2002-06-28 | 2005-04-21 | Xcyte Therapies, Inc. | Compositions and methods for eliminating undesired subpopulations of T cells in patients with immunological defects related to autoimmunity and organ or hematopoietic stem cell transplantation |
| PL1897548T5 (pl) | 2003-02-28 | 2025-03-17 | The Johns Hopkins University | Regulacja komórek T |
| KR20060039940A (ko) | 2003-08-22 | 2006-05-09 | 다카라 바이오 가부시키가이샤 | 세포 상해성 림프구의 제조 방법 |
| CN101243187B (zh) | 2005-08-17 | 2012-07-11 | 宝生物工程株式会社 | 淋巴细胞的制备方法 |
| JPWO2007142300A1 (ja) | 2006-06-09 | 2009-10-29 | タカラバイオ株式会社 | リンパ球の製造方法 |
| AU2008262487B2 (en) * | 2007-05-23 | 2013-10-31 | Sangamo Therapeutics, Inc. | Methods and compositions for increased transgene expression |
| WO2013094988A1 (fr) * | 2011-12-22 | 2013-06-27 | Mogam Biotechnology Research Institute | Procédé pour produire des cellules tueuses naturelles, cellules tueuses naturelles produites de cette façon, et composition pour traiter des cancers et des maladies infectieuses contenant les mêmes |
| JP2016539929A (ja) | 2013-10-25 | 2016-12-22 | ボード・オブ・リージエンツ，ザ・ユニバーシテイ・オブ・テキサス・システム | 免疫療法のためのポリクローナルγδＴ細胞 |
| LT3689899T (lt) | 2014-04-25 | 2021-12-10 | 2Seventy Bio, Inc. | Mnd promotorių chimerinių antigenų receptoriai |
| RS60544B1 (sr) * | 2014-04-25 | 2020-08-31 | Bluebird Bio Inc | Poboljšani metodi za proizvodnju adoptivnih ćelijskih terapija |
| MX380612B (es) | 2014-06-06 | 2025-03-12 | 2Seventy Bio Inc | Composiciones de celulas t mejoradas. |
| RU2747457C2 (ru) | 2014-07-24 | 2021-05-05 | Блубёрд Био, Инк. | Химерные антигенные рецепторы к bcma |
| KR101697473B1 (ko) | 2014-11-26 | 2017-01-18 | 주식회사 녹십자랩셀 | T 세포를 이용한 자연살해세포의 배양방법 |
| PL3230321T3 (pl) | 2014-12-12 | 2020-03-31 | Bluebird Bio, Inc. | Chimeryczne receptory antygenowe bcma |
| WO2017099712A1 (fr) | 2015-12-07 | 2017-06-15 | Bluebird Bio, Inc. | Compositions de lymphocytes t améliorées |
| US12109234B2 (en) | 2016-11-04 | 2024-10-08 | 2Seventy Bio, Inc. | Anti-BCMA CAR T cell compositions |
| EP3633029A4 (fr) | 2017-05-26 | 2021-06-09 | Green Cross Lab Cell Corporation | Procédé de culture de cellules tueuses naturelles au moyen d'un lymphocyte t transformé |
| CN113383069B (zh) | 2018-11-14 | 2024-12-13 | Gc细胞治疗 | 使用转化的t细胞培养脐带血来源的自然杀伤细胞的方法 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| JPH02502424A (ja) * | 1987-03-11 | 1990-08-09 | ザ・チルドレンズ・ホスピタル，インコーポレイテッド | 抗原特異的ｔセルラインの調製法並びに治療のためのその使用 |
| US5057423A (en) | 1987-12-18 | 1991-10-15 | University Of Pittsburgh | Method for the preparation of pure LAK-active lymphocytes |
| ZA91463B (en) * | 1990-01-25 | 1992-09-30 | Bristol Myers Squibb Co | Method of activating cytolytic activity of lymphocytes using anti-cd28 antibody |
| WO1992005794A1 (fr) * | 1990-09-28 | 1992-04-16 | Immunex Corporation | Methode servant a produire des lymphocytes t cytotoxiques th-independants |
| AU650085B2 (en) * | 1990-11-13 | 1994-06-09 | Immunex Corporation | Bifunctional selectable fusion genes |
| DE4410136A1 (de) * | 1993-03-31 | 1994-10-06 | Boehringer Mannheim Gmbh | Tumorizide T-Lymphozyten |
| US5827642A (en) * | 1994-08-31 | 1998-10-27 | Fred Hutchinson Cancer Research Center | Rapid expansion method ("REM") for in vitro propagation of T lymphocytes |
-
1997
- 1997-03-03 CA CA002247131A patent/CA2247131A1/fr active Pending
- 1997-03-03 IL IL12588497A patent/IL125884A0/xx unknown
- 1997-03-03 AU AU21947/97A patent/AU726198B2/en not_active Ceased
- 1997-03-03 WO PCT/US1997/003293 patent/WO1997032970A1/fr not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| WO1997032970A1 (fr) | 1997-09-12 |
| AU2194797A (en) | 1997-09-22 |
| IL125884A0 (en) | 1999-04-11 |
| AU726198B2 (en) | 2000-11-02 |
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