CA2261020C - Preparation de mutants auxotrophes de pichia methanolica - Google Patents

Preparation de mutants auxotrophes de pichia methanolica Download PDF

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Publication number
CA2261020C
CA2261020C CA002261020A CA2261020A CA2261020C CA 2261020 C CA2261020 C CA 2261020C CA 002261020 A CA002261020 A CA 002261020A CA 2261020 A CA2261020 A CA 2261020A CA 2261020 C CA2261020 C CA 2261020C
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CA
Canada
Prior art keywords
cells
dna
methanolica
culturing
ade
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA002261020A
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English (en)
Other versions
CA2261020A1 (fr
Inventor
Christopher K. Raymond
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Zymogenetics Inc
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Zymogenetics Inc
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Publication date
Priority claimed from US08/703,808 external-priority patent/US5736383A/en
Application filed by Zymogenetics Inc filed Critical Zymogenetics Inc
Publication of CA2261020A1 publication Critical patent/CA2261020A1/fr
Application granted granted Critical
Publication of CA2261020C publication Critical patent/CA2261020C/fr
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Procédés de préparation de cellules de Pichia methanolica à mutations auxotrophes, qui comprennent les étapes suivantes: (a) exposition de cellules de P. methanolica à des conditions de mutagenèse, (b) mise en culture de cellules issues de (a) dans un milieu riche pour l'établissement et la réplication de mutations au moins dans une partie des cellules, (c) mise en culture de cellules de (b) dans un milieu de culture déficient en azote assimilable pour appauvrir les unités cellulaires de stockage d'azote, (d) mise en culture de cellules de (c) dans un milieu de culture définie renfermant une source d'azote inorganique et une quantité de nystatine suffisante pour tuer les cellules de P. methanolica en développement afin de sélectionner les cellules à déficience en gènes nutritionnels; et (e) mise en culture de cellules sélectionnées à partir de (d) dans un milieu de culture riche.
CA002261020A 1996-07-17 1997-07-14 Preparation de mutants auxotrophes de pichia methanolica Expired - Fee Related CA2261020C (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US68350096A 1996-07-17 1996-07-17
US60/042,910 1996-07-17
US08/703,808 US5736383A (en) 1996-08-26 1996-08-26 Preparation of Pichia methanolica auxotrophic mutants
US08/703,808 1996-08-26
PCT/US1997/012582 WO1998002536A2 (fr) 1996-07-17 1997-07-14 PREPARATION DE MUTANTS AUXOTROPHES DE $i(PICHIA METHANOLICA)

Publications (2)

Publication Number Publication Date
CA2261020A1 CA2261020A1 (fr) 1998-01-22
CA2261020C true CA2261020C (fr) 2004-06-08

Family

ID=27103122

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002261020A Expired - Fee Related CA2261020C (fr) 1996-07-17 1997-07-14 Preparation de mutants auxotrophes de pichia methanolica

Country Status (6)

Country Link
JP (1) JP2002514049A (fr)
CN (1) CN1238806A (fr)
AU (1) AU708572B2 (fr)
CA (1) CA2261020C (fr)
IL (1) IL128072A0 (fr)
WO (1) WO1998002536A2 (fr)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69941126D1 (de) 1998-09-23 2009-08-27 Zymogenetics Inc Cytokinerezeptor zalpha11
US7833529B1 (en) 1999-01-07 2010-11-16 Zymogenetics, Inc. Methods for inhibiting B lymphocyte proliferation with soluble ztnf4 receptor
SI1642972T1 (sl) 1999-01-07 2010-05-31 Zymogenetics Inc Terapevtske uporabe BR X topnih receptorjev
KR100743640B1 (ko) 1999-03-09 2007-07-27 지모제넥틱스, 인코포레이티드 신규한 사이토킨 ｚａｌｐｈａ 11 리간드
EP2241623A3 (fr) 1999-07-07 2010-12-01 ZymoGenetics, Inc. Anti-corps monoclonal contre un récepteur de cytokine humain
AU2292601A (en) 1999-12-23 2001-07-03 Zymogenetics Inc. Novel cytokine zcyto18
CA2412239C (fr) 2000-06-26 2013-05-28 Zymogenetics, Inc. Zcytor17, recepteur de la cytokine
WO2002066516A2 (fr) 2001-02-20 2002-08-29 Zymogenetics, Inc. Anticorps liant a la fois bcma et taci
BRPI0209933B8 (pt) 2001-05-24 2021-05-25 Zymogenetics Inc proteína de fusão, e, molécula de ácido nucleico
ES2411007T3 (es) 2001-10-10 2013-07-04 Novo Nordisk A/S Remodelación y glicoconjugación de péptidos
ES2334338T3 (es) 2001-11-05 2010-03-09 Zymogenetics, Inc. Antagonistas de il-21.
EP2840089A1 (fr) 2002-01-18 2015-02-25 ZymoGenetics, Inc. Multimères Zcytor17 du récepteur de la cytokine
DK1961811T3 (da) 2002-01-18 2010-11-08 Zymogenetics Inc Cytokinligand til behandling af asthma og luftvejs-hyper-responsivitet
CN1659274A (zh) 2002-04-19 2005-08-24 津莫吉尼蒂克斯公司 细胞因子受体
PL1615945T3 (pl) 2003-04-09 2012-03-30 Ratiopharm Gmbh Sposoby glikopegylacji i białka/peptydy wytwarzane tymi sposobami
WO2006013072A2 (fr) 2004-08-02 2006-02-09 Basf Plant Science Gmbh Procede pour l'isolement de sequences de terminaison de transcription
CN100347287C (zh) * 2004-09-30 2007-11-07 汪和睦 一种重组多形汉逊酵母菌及其构建方法与应用
EP1856156A2 (fr) 2005-02-08 2007-11-21 ZymoGenetics, Inc. Anticorps et partenaires de liaison anti-il-20, anti-il-22 et anti-il-22ra, et methodes d'utilisation de ces anticorps et partenaires de liaison dans des processus inflammatoires
EP1891107B1 (fr) 2005-05-12 2011-07-06 ZymoGenetics, Inc. Compositions et procedes permettant de moduler des reponses immunitaires
EP1922080A2 (fr) 2005-08-09 2008-05-21 ZymoGenetics, Inc. Procedes destines au traitement des malignites des lymphocytes b au moyen d'une molecule de fusion taci-ig
WO2007019573A2 (fr) 2005-08-09 2007-02-15 Zymogenetics, Inc. Procedes pour le traitement et la prevention de proliferation cellulaire anormale par le biais de molecules de fusion taci
JP2009510093A (ja) 2005-09-28 2009-03-12 ザイモジェネティクス， インコーポレイテッド Ｉｌ−１７ａおよびｉｌ−１７ｆアンタゴニストならびにその使用方法
BRPI0711823A2 (pt) 2006-05-15 2012-01-17 Ares Trading Sa métodos para tratamento de doenças auto-imunes com uma molécula de fusão taci-ig
WO2009065415A1 (fr) 2007-11-21 2009-05-28 Roskilde Universitet Polypeptides comprenant une activité de liaison à la glace
KR101720760B1 (ko) 2008-01-25 2017-03-28 오르후스 우니베르시테트 Ｉｇｆｂｐ-4에 대한 ｐａｐｐ-ａ 활성의 선택적 엑소사이트 억제
ES2410579T5 (es) 2008-12-16 2021-10-04 Novartis Ag Sistemas de despliegue de levaduras
EP3597764A3 (fr) 2012-02-01 2020-05-06 SGI-DNA, Inc. Matériau et procédés de synthèse de molécules d'acide nucléique à réduction d'erreurs
WO2014202089A2 (fr) 2013-06-18 2014-12-24 Roskilde Universitet Variants de polypeptides antigel
CN111157680B (zh) * 2019-12-31 2021-10-26 北京辰安科技股份有限公司 室内挥发性物质的泄漏溯源方法及装置
CN116064597B (zh) * 2023-01-17 2024-04-26 北京大学 通过自主复制rna实现哺乳动物细胞中的定向进化和达尔文适应

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5965389A (en) * 1995-11-09 1999-10-12 Zymogenetics, Inc. Production of GAD65 in methylotrophic yeast

Also Published As

Publication number Publication date
CN1238806A (zh) 1999-12-15
WO1998002536A2 (fr) 1998-01-22
IL128072A0 (en) 1999-11-30
AU3885697A (en) 1998-02-09
WO1998002536A3 (fr) 1998-02-26
CA2261020A1 (fr) 1998-01-22
JP2002514049A (ja) 2002-05-14
AU708572B2 (en) 1999-08-05

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