CA2261151C - Transformation de pichia methanolica - Google Patents
Transformation de pichia methanolica Download PDFInfo
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- CA2261151C CA2261151C CA002261151A CA2261151A CA2261151C CA 2261151 C CA2261151 C CA 2261151C CA 002261151 A CA002261151 A CA 002261151A CA 2261151 A CA2261151 A CA 2261151A CA 2261151 C CA2261151 C CA 2261151C
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
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Abstract
Procédés pour la transformation de Pichia méthanolica, et molécules d'ADN utiles dans cette transformation. En présence de molécules d'ADN, les cellules de P. méthanolica sont exposées à un champ électrique pulsé dont l'intensité varie entre 2,5 et 4,5 kV/cm, moyennant une durée d'impulsion comprise entre 1 et 40 millisecondes, et les molécules d'ADN sont ainsi introduites dans les cellules. Ces molécules d'ADN peuvent comprendre une unité d'expression renfermant un promoteur de transcription de gène P. méthanolica lié de manière opérationnelle à un segment qui code un polypeptide ou une protéine présentant de l'intérêt. Les molécules d'ADN peuvent aussi coder un marqueur sélectionnable, par exemple le gène ADE2 de P. méthanolica. Il est possible d'utiliser les cellules ainsi transformées dans les systèmes de production servant à préparer les protéines qui ont une certaine importance commerciale.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US68350096A | 1996-07-17 | 1996-07-17 | |
| US08/683,500 | 1996-07-17 | ||
| PCT/US1997/012581 WO1998002565A1 (fr) | 1996-07-17 | 1997-07-14 | TRANSFORMATION DE $i(PICHIA METHANOLICA) |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2261151A1 CA2261151A1 (fr) | 1998-01-22 |
| CA2261151C true CA2261151C (fr) | 2003-09-16 |
Family
ID=24744316
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002261151A Expired - Fee Related CA2261151C (fr) | 1996-07-17 | 1997-07-14 | Transformation de pichia methanolica |
Country Status (5)
| Country | Link |
|---|---|
| JP (1) | JP2002515746A (fr) |
| AU (1) | AU718510B2 (fr) |
| CA (1) | CA2261151C (fr) |
| IL (1) | IL128073A0 (fr) |
| WO (1) | WO1998002565A1 (fr) |
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| AU772172B2 (en) | 1998-09-23 | 2004-04-08 | Zymogenetics Inc. | Cytokine receptor zalpha11 |
| TR200101998T2 (tr) | 1999-01-07 | 2002-06-21 | Zymogenetics, Inc. | Çözünür reseptör BR 43x2 ve kullanım metodları |
| US7833529B1 (en) | 1999-01-07 | 2010-11-16 | Zymogenetics, Inc. | Methods for inhibiting B lymphocyte proliferation with soluble ztnf4 receptor |
| DK1165791T3 (da) | 1999-03-09 | 2008-02-11 | Zymogenetics Inc | Humant cytokin som zalpha-receptor-ligand og anvendelse deraf |
| AU3910000A (en) * | 1999-03-22 | 2000-10-09 | Zymogenetics Inc. | Improved methods for producing proteins in transformed (pichia) |
| US6258559B1 (en) | 1999-03-22 | 2001-07-10 | Zymogenetics, Inc. | Method for producing proteins in transformed Pichia |
| DE60045931D1 (de) | 1999-07-07 | 2011-06-16 | Zymogenetics Inc | Menschliches Rezeptor für Zytokine |
| JP2003524415A (ja) | 1999-12-23 | 2003-08-19 | ザイモジェネティクス，インコーポレイティド | サイトカインｚｃｙｔｏ１８ |
| WO2002000721A2 (fr) | 2000-06-26 | 2002-01-03 | Zymogenetics, Inc. | Zcytor17, recepteur de la cytokine |
| CA2438682A1 (fr) | 2001-02-20 | 2002-08-29 | Zymogenetics, Inc. | Anticorps liant a la fois bcma et taci |
| EA010594B1 (ru) | 2001-05-24 | 2008-10-30 | Займодженетикс, Инк. | Слитые белки taci-иммуноглобулина |
| EP2042196B1 (fr) | 2001-10-10 | 2016-07-13 | ratiopharm GmbH | Remodelage et glycoconjugation de facteur stimulant les colonies de granulocytes (G-CSF) |
| MXPA04004167A (es) | 2001-11-05 | 2004-07-08 | Zymogenetics Inc | Antagonistas de interleucina-21. |
| ES2382800T3 (es) | 2002-01-18 | 2012-06-13 | Zymogenetics, Inc. | Multímeros Zcytor17 receptores de citocinas |
| ES2377188T3 (es) | 2002-01-18 | 2012-03-23 | Zymogenetics, Inc. | Nuevo ligando de citocina ZCYTOR17 |
| EP1497415B1 (fr) | 2002-04-19 | 2010-12-29 | ZymoGenetics, L.L.C. | Methodes d'identification ou modulation de l'interaction d'un recepteur de cytokine et son ligand |
| NZ542586A (en) | 2003-04-09 | 2009-11-27 | Biogenerix Ag | Method of forming a covalent conjugate between a G-CSF peptide and PEG |
| EP1781784A2 (fr) | 2004-08-02 | 2007-05-09 | BASF Plant Science GmbH | Procede pour l'isolement de sequences de terminaison de transcription |
| EP2192132A3 (fr) | 2005-02-08 | 2010-08-18 | ZymoGenetics, Inc. | Anticorps et partenaires de liaison anti-il-20, anti-il-22 et anti-il-22ra, et methodes d'utilisation de ces anticorps et partenaires de liaison dans des processus inflammatoires |
| JP2008544746A (ja) | 2005-05-12 | 2008-12-11 | ザイモジェネティクス， インコーポレイテッド | 免疫応答を調節するための組成物および方法 |
| WO2007019573A2 (fr) | 2005-08-09 | 2007-02-15 | Zymogenetics, Inc. | Procedes pour le traitement et la prevention de proliferation cellulaire anormale par le biais de molecules de fusion taci |
| CN101262876A (zh) | 2005-08-09 | 2008-09-10 | 酶遗传学股份有限公司 | 用taci-ig融合分子治疗b细胞恶性肿瘤的方法 |
| EP1931697B1 (fr) | 2005-09-28 | 2010-09-15 | ZymoGenetics, Inc. | Antagonistes anti il-17a et anti il-17f et leurs methodes d'utilisation |
| CN101489573B (zh) | 2006-05-15 | 2013-05-22 | 阿雷斯贸易股份有限公司 | 用TACI-Ig融合分子治疗自身免疫疾病的方法 |
| EP2602263B1 (fr) | 2007-11-21 | 2019-07-17 | Roskilde Universitet | Polypeptides comprenant une activité de liaison à la glace |
| JP5960947B2 (ja) * | 2007-12-19 | 2016-08-02 | グライコフィ， インコーポレイテッド | タンパク質の製造のための酵母株 |
| MX2010008083A (es) | 2008-01-25 | 2010-12-20 | Univ Aarhus | Inhibicion de exositios selectivos de actividad papp-a contra igfbp-4. |
| CN102245771B (zh) | 2008-12-16 | 2013-04-10 | 诺瓦提斯公司 | 酵母展示系统 |
| SG10201606285YA (en) | 2012-02-01 | 2016-09-29 | Synthetic Genomics Inc | Materials and methods for the synthesis of error-minimized nucleic acid molecules |
| WO2014202089A2 (fr) | 2013-06-18 | 2014-12-24 | Roskilde Universitet | Variants de polypeptides antigel |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4857467A (en) * | 1986-07-23 | 1989-08-15 | Phillips Petroleum Company | Carbon and energy source markers for transformation of strains of the genes Pichia |
| WO1997017450A2 (fr) * | 1995-11-09 | 1997-05-15 | Zymogenetics, Inc. | Compositions et procedes de production de polypeptides heterologues sur pichia methanolica |
-
1997
- 1997-07-14 WO PCT/US1997/012581 patent/WO1998002565A1/fr not_active Ceased
- 1997-07-14 IL IL12807397A patent/IL128073A0/xx unknown
- 1997-07-14 CA CA002261151A patent/CA2261151C/fr not_active Expired - Fee Related
- 1997-07-14 JP JP50632298A patent/JP2002515746A/ja not_active Ceased
- 1997-07-14 AU AU38039/97A patent/AU718510B2/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| AU3803997A (en) | 1998-02-09 |
| AU718510B2 (en) | 2000-04-13 |
| CA2261151A1 (fr) | 1998-01-22 |
| WO1998002565A1 (fr) | 1998-01-22 |
| IL128073A0 (en) | 1999-11-30 |
| JP2002515746A (ja) | 2002-05-28 |
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