CA2272788A1 - Moyens et methodes pour transformer les mitochondries des vegetaux - Google Patents

Moyens et methodes pour transformer les mitochondries des vegetaux Download PDF

Info

Publication number: CA2272788A1
Authority: CA; Canada
Prior art keywords: mitochondria; nucleotide; dna; plant cell; plant
Prior art date: 1999-06-11
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Abandoned

Application number

CA 2272788

Other languages

English (en)

Inventor

Renate Luehrs

Steffen Backert

Thomas Boerner

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

GARCHING INNOVATION GmbH

Original Assignee

GARCHING INNOVATION GMBH

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1999-06-11

Filing date

1999-06-11

Publication date

2000-12-11

1999-06-11 Application filed by GARCHING INNOVATION GMBH filed Critical GARCHING INNOVATION GMBH

1999-06-11 Priority to CA 2272788 priority Critical patent/CA2272788A1/fr

2000-12-11 Publication of CA2272788A1 publication Critical patent/CA2272788A1/fr

Status Abandoned legal-status Critical Current

Links

210000003470 mitochondria Anatomy 0.000 title claims abstract description 158
238000000034 method Methods 0.000 title claims abstract description 60
230000009466 transformation Effects 0.000 title description 21
108020004414 DNA Proteins 0.000 claims abstract description 186
210000004027 cell Anatomy 0.000 claims abstract description 130
108090000623 proteins and genes Proteins 0.000 claims abstract description 92
108700039691 Genetic Promoter Regions Proteins 0.000 claims abstract description 51
230000010076 replication Effects 0.000 claims abstract description 47
230000009261 transgenic effect Effects 0.000 claims abstract description 45
239000012634 fragment Substances 0.000 claims abstract description 40
102000004169 proteins and genes Human genes 0.000 claims abstract description 38
108091026890 Coding region Proteins 0.000 claims abstract description 34
230000014509 gene expression Effects 0.000 claims abstract description 29
238000013518 transcription Methods 0.000 claims abstract description 15
230000035897 transcription Effects 0.000 claims abstract description 15
238000004519 manufacturing process Methods 0.000 claims abstract description 14
230000001172 regenerating effect Effects 0.000 claims abstract description 9
108700026244 Open Reading Frames Proteins 0.000 claims abstract description 8
108700007698 Genetic Terminator Regions Proteins 0.000 claims abstract description 5
241000196324 Embryophyta Species 0.000 claims description 225
239000002773 nucleotide Substances 0.000 claims description 99
125000003729 nucleotide group Chemical group 0.000 claims description 99
239000013612 plasmid Substances 0.000 claims description 81
210000001938 protoplast Anatomy 0.000 claims description 67
230000002438 mitochondrial effect Effects 0.000 claims description 62
238000011144 upstream manufacturing Methods 0.000 claims description 19
108090000765 processed proteins & peptides Proteins 0.000 claims description 17
102000004196 processed proteins & peptides Human genes 0.000 claims description 15
102000053602 DNA Human genes 0.000 claims description 14
229920001184 polypeptide Polymers 0.000 claims description 14
239000003550 marker Substances 0.000 claims description 10
240000006677 Vicia faba Species 0.000 claims description 7
235000010749 Vicia faba Nutrition 0.000 claims description 7
101150071146 COX2 gene Proteins 0.000 claims description 6
240000006122 Chenopodium album Species 0.000 claims description 6
235000009344 Chenopodium album Nutrition 0.000 claims description 6
230000001086 cytosolic effect Effects 0.000 claims description 6
101150078442 RPL5 gene Proteins 0.000 claims description 5
206010021929 Infertility male Diseases 0.000 claims description 4
208000007466 Male Infertility Diseases 0.000 claims description 4
238000004520 electroporation Methods 0.000 claims description 4
230000002401 inhibitory effect Effects 0.000 claims description 4
150000005846 sugar alcohols Polymers 0.000 claims description 3
239000005090 green fluorescent protein Substances 0.000 description 26
108091032973 (ribonucleotides)n+m Proteins 0.000 description 20
244000061176 Nicotiana tabacum Species 0.000 description 17
235000002637 Nicotiana tabacum Nutrition 0.000 description 17
239000000243 solution Substances 0.000 description 16
108010043121 Green Fluorescent Proteins Proteins 0.000 description 12
102000004144 Green Fluorescent Proteins Human genes 0.000 description 12
101100324954 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) oli gene Proteins 0.000 description 12
240000002791 Brassica napus Species 0.000 description 11
108020005196 Mitochondrial DNA Proteins 0.000 description 11
101150093272 ATP9 gene Proteins 0.000 description 10
108091034117 Oligonucleotide Proteins 0.000 description 10
238000002105 Southern blotting Methods 0.000 description 10
238000002474 experimental method Methods 0.000 description 9
235000011293 Brassica napus Nutrition 0.000 description 8
210000000805 cytoplasm Anatomy 0.000 description 8
230000000977 initiatory effect Effects 0.000 description 8
239000000523 sample Substances 0.000 description 7
210000003763 chloroplast Anatomy 0.000 description 6
238000000338 in vitro Methods 0.000 description 6
230000000749 insecticidal effect Effects 0.000 description 6
108020004705 Codon Proteins 0.000 description 5
102000004190 Enzymes Human genes 0.000 description 5
108090000790 Enzymes Proteins 0.000 description 5
108091028043 Nucleic acid sequence Proteins 0.000 description 5
238000012408 PCR amplification Methods 0.000 description 5
239000002202 Polyethylene glycol Substances 0.000 description 5
229930006000 Sucrose Natural products 0.000 description 5
CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 5
229940088598 enzyme Drugs 0.000 description 5
230000035772 mutation Effects 0.000 description 5
102000039446 nucleic acids Human genes 0.000 description 5
108020004707 nucleic acids Proteins 0.000 description 5
150000007523 nucleic acids Chemical class 0.000 description 5
229920001223 polyethylene glycol Polymers 0.000 description 5
230000001105 regulatory effect Effects 0.000 description 5
239000005720 sucrose Substances 0.000 description 5
238000012546 transfer Methods 0.000 description 5
FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 4
101150066002 GFP gene Proteins 0.000 description 4
229930195725 Mannitol Natural products 0.000 description 4
101100271432 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) atp-9 gene Proteins 0.000 description 4
240000004808 Saccharomyces cerevisiae Species 0.000 description 4
108091081024 Start codon Proteins 0.000 description 4
239000000594 mannitol Substances 0.000 description 4
235000010355 mannitol Nutrition 0.000 description 4
239000000047 product Substances 0.000 description 4
230000003362 replicative effect Effects 0.000 description 4
210000001519 tissue Anatomy 0.000 description 4
229920000936 Agarose Polymers 0.000 description 3
108020004998 Chloroplast DNA Proteins 0.000 description 3
101000635955 Homo sapiens Myelin P2 protein Proteins 0.000 description 3
102100030738 Myelin P2 protein Human genes 0.000 description 3
239000002253 acid Substances 0.000 description 3
230000000295 complement effect Effects 0.000 description 3
238000010276 construction Methods 0.000 description 3
230000004927 fusion Effects 0.000 description 3
238000009396 hybridization Methods 0.000 description 3
238000002955 isolation Methods 0.000 description 3
239000000463 material Substances 0.000 description 3
239000008188 pellet Substances 0.000 description 3
230000008929 regeneration Effects 0.000 description 3
238000011069 regeneration method Methods 0.000 description 3
230000000241 respiratory effect Effects 0.000 description 3
238000003757 reverse transcription PCR Methods 0.000 description 3
239000000725 suspension Substances 0.000 description 3
239000003053 toxin Substances 0.000 description 3
231100000765 toxin Toxicity 0.000 description 3
108700012359 toxins Proteins 0.000 description 3
238000013519 translation Methods 0.000 description 3
239000013598 vector Substances 0.000 description 3
241000193388 Bacillus thuringiensis Species 0.000 description 2
235000004977 Brassica sinapistrum Nutrition 0.000 description 2
UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 2
108010059892 Cellulase Proteins 0.000 description 2
241000195585 Chlamydomonas Species 0.000 description 2
108020004638 Circular DNA Proteins 0.000 description 2
SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 description 2
VZCYOOQTPOCHFL-OWOJBTEDSA-N Fumaric acid Chemical compound OC(=O)\C=C\C(O)=O VZCYOOQTPOCHFL-OWOJBTEDSA-N 0.000 description 2
241000238631 Hexapoda Species 0.000 description 2
102000006404 Mitochondrial Proteins Human genes 0.000 description 2
108010058682 Mitochondrial Proteins Proteins 0.000 description 2
240000007594 Oryza sativa Species 0.000 description 2
235000007164 Oryza sativa Nutrition 0.000 description 2
108700001094 Plant Genes Proteins 0.000 description 2
235000019057 Raphanus caudatus Nutrition 0.000 description 2
244000088415 Raphanus sativus Species 0.000 description 2
235000011380 Raphanus sativus Nutrition 0.000 description 2
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
241000607479 Yersinia pestis Species 0.000 description 2
240000008042 Zea mays Species 0.000 description 2
JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 2
150000007513 acids Chemical class 0.000 description 2
238000004458 analytical method Methods 0.000 description 2
238000009395 breeding Methods 0.000 description 2
230000001488 breeding effect Effects 0.000 description 2
239000001110 calcium chloride Substances 0.000 description 2
235000011148 calcium chloride Nutrition 0.000 description 2
229910001628 calcium chloride Inorganic materials 0.000 description 2
229940106157 cellulase Drugs 0.000 description 2
238000005119 centrifugation Methods 0.000 description 2
239000007795 chemical reaction product Substances 0.000 description 2
KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Natural products OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 2
238000012411 cloning technique Methods 0.000 description 2
244000038559 crop plants Species 0.000 description 2
101150102059 cry3Aa gene Proteins 0.000 description 2
230000007123 defense Effects 0.000 description 2
230000002950 deficient Effects 0.000 description 2
238000011161 development Methods 0.000 description 2
230000018109 developmental process Effects 0.000 description 2
235000021186 dishes Nutrition 0.000 description 2
230000000694 effects Effects 0.000 description 2
108020001507 fusion proteins Proteins 0.000 description 2
102000037865 fusion proteins Human genes 0.000 description 2
230000002068 genetic effect Effects 0.000 description 2
238000003780 insertion Methods 0.000 description 2
230000037431 insertion Effects 0.000 description 2
239000000543 intermediate Substances 0.000 description 2
150000002500 ions Chemical class 0.000 description 2
239000007788 liquid Substances 0.000 description 2
230000008774 maternal effect Effects 0.000 description 2
210000004940 nucleus Anatomy 0.000 description 2
210000003463 organelle Anatomy 0.000 description 2
210000002706 plastid Anatomy 0.000 description 2
230000010152 pollination Effects 0.000 description 2
238000002741 site-directed mutagenesis Methods 0.000 description 2
238000012360 testing method Methods 0.000 description 2
230000005030 transcription termination Effects 0.000 description 2
235000013311 vegetables Nutrition 0.000 description 2
BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 description 1
102000040650 (ribonucleotides)n+m Human genes 0.000 description 1
239000005631 2,4-Dichlorophenoxyacetic acid Substances 0.000 description 1
108091006112 ATPases Proteins 0.000 description 1
102000057290 Adenosine Triphosphatases Human genes 0.000 description 1
108020005544 Antisense RNA Proteins 0.000 description 1
101000977023 Azospirillum brasilense Uncharacterized 17.8 kDa protein in nodG 5'region Proteins 0.000 description 1
101000961984 Bacillus thuringiensis Uncharacterized 30.3 kDa protein Proteins 0.000 description 1
241000894006 Bacteria Species 0.000 description 1
241000219310 Beta vulgaris subsp. vulgaris Species 0.000 description 1
241000219198 Brassica Species 0.000 description 1
235000011331 Brassica Nutrition 0.000 description 1
235000006008 Brassica napus var napus Nutrition 0.000 description 1
108090000994 Catalytic RNA Proteins 0.000 description 1
102000053642 Catalytic RNA Human genes 0.000 description 1
241000219312 Chenopodium Species 0.000 description 1
241000195597 Chlamydomonas reinhardtii Species 0.000 description 1
101000703651 Chlamydomonas reinhardtii Arylsulfatase Proteins 0.000 description 1
108700031407 Chloroplast Genes Proteins 0.000 description 1
244000298479 Cichorium intybus Species 0.000 description 1
235000007542 Cichorium intybus Nutrition 0.000 description 1
229920000742 Cotton Polymers 0.000 description 1
101710151559 Crystal protein Proteins 0.000 description 1
GUBGYTABKSRVRQ-CUHNMECISA-N D-Cellobiose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-CUHNMECISA-N 0.000 description 1
FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 1
SHZGCJCMOBCMKK-UHFFFAOYSA-N D-mannomethylose Natural products CC1OC(O)C(O)C(O)C1O SHZGCJCMOBCMKK-UHFFFAOYSA-N 0.000 description 1
WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 1
HMFHBZSHGGEWLO-SOOFDHNKSA-N D-ribofuranose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H]1O HMFHBZSHGGEWLO-SOOFDHNKSA-N 0.000 description 1
101000644901 Drosophila melanogaster Putative 115 kDa protein in type-1 retrotransposable element R1DM Proteins 0.000 description 1
101000747702 Enterobacteria phage N4 Uncharacterized protein Gp2 Proteins 0.000 description 1
101000758599 Escherichia coli Uncharacterized 14.7 kDa protein Proteins 0.000 description 1
241000206602 Eukaryota Species 0.000 description 1
108091029865 Exogenous DNA Proteins 0.000 description 1
229930091371 Fructose Natural products 0.000 description 1
239000005715 Fructose Substances 0.000 description 1
RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
241000233866 Fungi Species 0.000 description 1
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
244000068988 Glycine max Species 0.000 description 1
235000010469 Glycine max Nutrition 0.000 description 1
241000219146 Gossypium Species 0.000 description 1
244000020551 Helianthus annuus Species 0.000 description 1
235000003222 Helianthus annuus Nutrition 0.000 description 1
101000958664 Homo sapiens Nucleus accumbens-associated protein 1 Proteins 0.000 description 1
101000631760 Homo sapiens Sodium channel protein type 1 subunit alpha Proteins 0.000 description 1
240000005979 Hordeum vulgare Species 0.000 description 1
235000007340 Hordeum vulgare Nutrition 0.000 description 1
206010020649 Hyperkeratosis Diseases 0.000 description 1
SHZGCJCMOBCMKK-JFNONXLTSA-N L-rhamnopyranose Chemical compound C[C@@H]1OC(O)[C@H](O)[C@H](O)[C@H]1O SHZGCJCMOBCMKK-JFNONXLTSA-N 0.000 description 1
PNNNRSAQSRJVSB-UHFFFAOYSA-N L-rhamnose Natural products CC(O)C(O)C(O)C(O)C=O PNNNRSAQSRJVSB-UHFFFAOYSA-N 0.000 description 1
101000768930 Lactococcus lactis subsp. cremoris Uncharacterized protein in pepC 5'region Proteins 0.000 description 1
240000008415 Lactuca sativa Species 0.000 description 1
235000003228 Lactuca sativa Nutrition 0.000 description 1
101000976302 Leptospira interrogans Uncharacterized protein in sph 3'region Proteins 0.000 description 1
101000778886 Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601) Uncharacterized protein LA_2151 Proteins 0.000 description 1
241000209510 Liliopsida Species 0.000 description 1
240000000894 Lupinus albus Species 0.000 description 1
235000010649 Lupinus albus Nutrition 0.000 description 1
235000007688 Lycopersicon esculentum Nutrition 0.000 description 1
241001465754 Metazoa Species 0.000 description 1
108091093105 Nuclear DNA Proteins 0.000 description 1
206010034133 Pathogen resistance Diseases 0.000 description 1
241001148062 Photorhabdus Species 0.000 description 1
229920002535 Polyethylene Glycol 1500 Polymers 0.000 description 1
238000010240 RT-PCR analysis Methods 0.000 description 1
108020004511 Recombinant DNA Proteins 0.000 description 1
PYMYPHUHKUWMLA-LMVFSUKVSA-N Ribose Natural products OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PYMYPHUHKUWMLA-LMVFSUKVSA-N 0.000 description 1
101001121571 Rice tungro bacilliform virus (isolate Philippines) Protein P2 Proteins 0.000 description 1
240000000111 Saccharum officinarum Species 0.000 description 1
235000007201 Saccharum officinarum Nutrition 0.000 description 1
101710169845 Sesquipedalian-2 Proteins 0.000 description 1
102100037576 Sestrin-2 Human genes 0.000 description 1
102100028910 Sodium channel protein type 1 subunit alpha Human genes 0.000 description 1
240000003768 Solanum lycopersicum Species 0.000 description 1
244000061456 Solanum tuberosum Species 0.000 description 1
235000002595 Solanum tuberosum Nutrition 0.000 description 1
101000818098 Spirochaeta aurantia Uncharacterized protein in trpE 3'region Proteins 0.000 description 1
101001026590 Streptomyces cinnamonensis Putative polyketide beta-ketoacyl synthase 2 Proteins 0.000 description 1
235000021536 Sugar beet Nutrition 0.000 description 1
101000750896 Synechococcus elongatus (strain PCC 7942 / FACHB-805) Uncharacterized protein Synpcc7942_2318 Proteins 0.000 description 1
108020005038 Terminator Codon Proteins 0.000 description 1
108091023040 Transcription factor Proteins 0.000 description 1
102000040945 Transcription factor Human genes 0.000 description 1
108700019146 Transgenes Proteins 0.000 description 1
235000021307 Triticum Nutrition 0.000 description 1
244000098338 Triticum aestivum Species 0.000 description 1
241000700605 Viruses Species 0.000 description 1
101000916321 Xenopus laevis Transposon TX1 uncharacterized 149 kDa protein Proteins 0.000 description 1
241000607757 Xenorhabdus Species 0.000 description 1
235000007244 Zea mays Nutrition 0.000 description 1
235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
235000002017 Zea mays subsp mays Nutrition 0.000 description 1
101000760088 Zymomonas mobilis subsp. mobilis (strain ATCC 10988 / DSM 424 / LMG 404 / NCIMB 8938 / NRRL B-806 / ZM1) 20.9 kDa protein Proteins 0.000 description 1
HMFHBZSHGGEWLO-UHFFFAOYSA-N alpha-D-Furanose-Ribose Natural products OCC1OC(O)C(O)C1O HMFHBZSHGGEWLO-UHFFFAOYSA-N 0.000 description 1
BJEPYKJPYRNKOW-UHFFFAOYSA-N alpha-hydroxysuccinic acid Natural products OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 description 1
239000003392 amylase inhibitor Substances 0.000 description 1
238000013459 approach Methods 0.000 description 1
PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 1
229940097012 bacillus thuringiensis Drugs 0.000 description 1
230000008901 benefit Effects 0.000 description 1
SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 1
230000001851 biosynthetic effect Effects 0.000 description 1
239000000969 carrier Substances 0.000 description 1
210000002421 cell wall Anatomy 0.000 description 1
238000006243 chemical reaction Methods 0.000 description 1
-1 citric acid sugars Chemical class 0.000 description 1
239000003184 complementary RNA Substances 0.000 description 1
229920001940 conductive polymer Polymers 0.000 description 1
239000000470 constituent Substances 0.000 description 1
235000005822 corn Nutrition 0.000 description 1
238000012217 deletion Methods 0.000 description 1
230000037430 deletion Effects 0.000 description 1
230000001419 dependent effect Effects 0.000 description 1
238000001514 detection method Methods 0.000 description 1
229940042399 direct acting antivirals protease inhibitors Drugs 0.000 description 1
230000027721 electron transport chain Effects 0.000 description 1
210000002257 embryonic structure Anatomy 0.000 description 1
230000006862 enzymatic digestion Effects 0.000 description 1
230000002255 enzymatic effect Effects 0.000 description 1
241001233957 eudicotyledons Species 0.000 description 1
210000003527 eukaryotic cell Anatomy 0.000 description 1
238000009363 floriculture Methods 0.000 description 1
239000001530 fumaric acid Substances 0.000 description 1
239000008103 glucose Substances 0.000 description 1
238000002744 homologous recombination Methods 0.000 description 1
230000006801 homologous recombination Effects 0.000 description 1
238000003898 horticulture Methods 0.000 description 1
238000011534 incubation Methods 0.000 description 1
238000009616 inductively coupled plasma Methods 0.000 description 1
229960000367 inositol Drugs 0.000 description 1
CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 description 1
230000010354 integration Effects 0.000 description 1
230000003993 interaction Effects 0.000 description 1
239000002502 liposome Substances 0.000 description 1
238000012423 maintenance Methods 0.000 description 1
239000001630 malic acid Substances 0.000 description 1
235000011090 malic acid Nutrition 0.000 description 1
108020004999 messenger RNA Proteins 0.000 description 1
230000037353 metabolic pathway Effects 0.000 description 1
239000002207 metabolite Substances 0.000 description 1
238000000520 microinjection Methods 0.000 description 1
239000003595 mist Substances 0.000 description 1
210000001700 mitochondrial membrane Anatomy 0.000 description 1
239000000203 mixture Substances 0.000 description 1
238000010369 molecular cloning Methods 0.000 description 1
229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
150000007524 organic acids Chemical class 0.000 description 1
235000005985 organic acids Nutrition 0.000 description 1
244000052769 pathogen Species 0.000 description 1
230000007170 pathology Effects 0.000 description 1
239000000137 peptide hydrolase inhibitor Substances 0.000 description 1
230000008569 process Effects 0.000 description 1
230000000644 propagated effect Effects 0.000 description 1
230000006798 recombination Effects 0.000 description 1
238000005215 recombination Methods 0.000 description 1
230000022532 regulation of transcription, DNA-dependent Effects 0.000 description 1
238000009877 rendering Methods 0.000 description 1
238000011160 research Methods 0.000 description 1
210000003705 ribosome Anatomy 0.000 description 1
108091092562 ribozyme Proteins 0.000 description 1
235000009566 rice Nutrition 0.000 description 1
239000011833 salt mixture Substances 0.000 description 1
239000002795 scorpion venom Substances 0.000 description 1
CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 description 1
239000013605 shuttle vector Substances 0.000 description 1
239000011780 sodium chloride Substances 0.000 description 1
239000002689 soil Substances 0.000 description 1
238000007711 solidification Methods 0.000 description 1
230000008023 solidification Effects 0.000 description 1
239000000600 sorbitol Substances 0.000 description 1
239000002708 spider venom Substances 0.000 description 1
239000010421 standard material Substances 0.000 description 1
238000006467 substitution reaction Methods 0.000 description 1
239000000758 substrate Substances 0.000 description 1
235000000346 sugar Nutrition 0.000 description 1
VUYXVWGKCKTUMF-UHFFFAOYSA-N tetratriacontaethylene glycol monomethyl ether Chemical compound COCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCO VUYXVWGKCKTUMF-UHFFFAOYSA-N 0.000 description 1
231100000331 toxic Toxicity 0.000 description 1
230000002588 toxic effect Effects 0.000 description 1
VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 description 1
238000011426 transformation method Methods 0.000 description 1
230000001131 transforming effect Effects 0.000 description 1
229940088594 vitamin Drugs 0.000 description 1
239000011782 vitamin Substances 0.000 description 1
229930003231 vitamin Natural products 0.000 description 1
235000013343 vitamin Nutrition 0.000 description 1
150000003722 vitamin derivatives Chemical class 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8214—Plastid transformation

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Genetics & Genomics (AREA)
Engineering & Computer Science (AREA)
Biomedical Technology (AREA)
Biotechnology (AREA)
Bioinformatics & Cheminformatics (AREA)
Organic Chemistry (AREA)
Chemical & Material Sciences (AREA)
General Engineering & Computer Science (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Cell Biology (AREA)
Microbiology (AREA)
Plant Pathology (AREA)
Molecular Biology (AREA)
Biophysics (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Physics & Mathematics (AREA)
Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)

CA 2272788 1999-06-11 1999-06-11 Moyens et methodes pour transformer les mitochondries des vegetaux Abandoned CA2272788A1 (fr)

Priority Applications (1)

Application Number	Priority Date	Filing Date	Title
CA 2272788 CA2272788A1 (fr)	1999-06-11	1999-06-11	Moyens et methodes pour transformer les mitochondries des vegetaux

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
CA 2272788 CA2272788A1 (fr)	1999-06-11	1999-06-11	Moyens et methodes pour transformer les mitochondries des vegetaux

Publications (1)

Publication Number	Publication Date
CA2272788A1 true CA2272788A1 (fr)	2000-12-11

Family

ID=29589052

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CA 2272788 Abandoned CA2272788A1 (fr)	1999-06-11	1999-06-11	Moyens et methodes pour transformer les mitochondries des vegetaux

Country Status (1)

Country	Link
CA (1)	CA2272788A1 (fr)

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
EP1463836A4 (fr) *	2001-12-13	2007-09-12	Univ Virginia	Transfection d'organelle induite par un vecteur
WO2009150441A1 (fr) *	2008-06-13	2009-12-17	University Of Stavanger	Transformation mitochondriale
GB2465748A (en) *	2008-11-25	2010-06-02	Algentech Ltd	Plant mitochondrial transformation method
US8062891B2 (en) *	2003-10-24	2011-11-22	Gencia Corporation	Nonviral vectors for delivering polynucleotides to plants
US8133733B2 (en)	2003-10-24	2012-03-13	Gencia Corporation	Nonviral vectors for delivering polynucleotides to target tissues
US8507277B2 (en)	2003-10-24	2013-08-13	Gencia Corporation	Nonviral vectors for delivering polynucleotides
US8541550B2 (en)	2003-10-24	2013-09-24	Gencia Corporation	Methods and compositions for delivering polynucleotides
US8927691B2 (en)	2003-10-24	2015-01-06	Gencia Corporation	Transducible polypeptides for modifying metabolism

1999
- 1999-06-11 CA CA 2272788 patent/CA2272788A1/fr not_active Abandoned

Cited By (14)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US7741112B2 (en)	2001-12-13	2010-06-22	University Of Virginia Patent Foundation	Vector mediated organelle transfection
EP1463836A4 (fr) *	2001-12-13	2007-09-12	Univ Virginia	Transfection d'organelle induite par un vecteur
US8133733B2 (en)	2003-10-24	2012-03-13	Gencia Corporation	Nonviral vectors for delivering polynucleotides to target tissues
US8062891B2 (en) *	2003-10-24	2011-11-22	Gencia Corporation	Nonviral vectors for delivering polynucleotides to plants
US8470972B2 (en)	2003-10-24	2013-06-25	Gencia Corporation	Nonviral vectors for delivering polynucleotides to plants
US8507277B2 (en)	2003-10-24	2013-08-13	Gencia Corporation	Nonviral vectors for delivering polynucleotides
US8541550B2 (en)	2003-10-24	2013-09-24	Gencia Corporation	Methods and compositions for delivering polynucleotides
US8927691B2 (en)	2003-10-24	2015-01-06	Gencia Corporation	Transducible polypeptides for modifying metabolism
US8952133B2 (en)	2003-10-24	2015-02-10	Gencia Corporation	Nonviral vectors for delivering polynucleotides to target tissue
WO2009150441A1 (fr) *	2008-06-13	2009-12-17	University Of Stavanger	Transformation mitochondriale
GB2465748A (en) *	2008-11-25	2010-06-02	Algentech Ltd	Plant mitochondrial transformation method
WO2010061187A3 (fr) *	2008-11-25	2010-07-29	Algentech Sas	Procédé de transformation de mitochondries de plante
GB2465748B (en) *	2008-11-25	2012-04-25	Algentech Sas	Plant cell transformation method
US9663792B2 (en)	2008-11-25	2017-05-30	Algentech Sas	Plant mitochondria transformation method

Legal Events

Date	Code	Title	Description
2002-06-11	FZDE	Dead

Publication	Publication Date	Title
US5451513A (en)	1995-09-19	Method for stably transforming plastids of multicellular plants
Singh et al.	2010	Plastid transformation in eggplant (Solanum melongena L.)
AU620039B2 (en)	1992-02-13	Inducible virus resistance in plants
US7557266B2 (en)	2009-07-07	Isolated polynucleotide molecules corresponding to mutant and wild-type alleles of the maize D9 gene and methods of use
JPS6094041A (ja)	1985-05-27	昆虫耐性植物
Asad et al.	2008	Silicon carbide whisker-mediated embryogenic callus transformation of cotton (Gossypium hirsutum L.) and regeneration of salt tolerant plants
US20230313212A1 (en)	2023-10-05	Plastid transformation by complementation of nuclear mutations
CN107267527A (zh)	2017-10-20	雄性育性的保持方法及其应用
Cao et al.	2004	Engineering higher yield and herbicide resistance in rice by Agrobacterium‐mediated multiple gene transformation
WO2019129145A1 (fr)	2019-07-04	Gène cmp1 de régulation de l'époque de floraison et constructions associées et applications correspondantes
US20240417703A1 (en)	2024-12-19	Error prone dna polymerase for organelle mutation
CA2272788A1 (fr)	2000-12-11	Moyens et methodes pour transformer les mitochondries des vegetaux
ES2388522T3 (es)	2012-10-16	Plantas transgénicas con una distribución controlada de un rasgo a una progenie
Shaya et al.	2012	Expression of Mitochondrial Gene Fragments within the Tapetum Induce Male Sterility by Limiting the Biogenesis of the Respiratory Machinery in Transgenic Tobacco F
US12595489B2 (en)	2026-04-07	Sterile genes and related constructs and applications thereof
HUP0000493A2 (hu)	2000-06-28	Nematódával indukálható, szabályozó DNS-szekvencia
CN101479294A (zh)	2009-07-08	对应于玉米d9基因的突变等位基因和野生型等位基因的分离的多核苷酸分子和使用方法
CN104650204B (zh)	2018-02-06	与水稻atp运输和叶绿体发育相关的蛋白及其编码基因和应用
EP2423316B1 (fr)	2016-11-16	Procédé pour déterminer la fréquence de la recombinaison meiotique dans les plantes
US20230225331A1 (en)	2023-07-20	Plant Endophytic Bacteria And Methods To Control Plant Pathogens And Pests
CN100424177C (zh)	2008-10-08	融合杀虫基因cryci及其应用
EP1896592B1 (fr)	2010-03-17	Procédés destinés à générer des plantes manifestant une conductance plasmodesmatale modifiée
Chikkala	2024	Production and Transformation of tobacco and Brassica containing Macrochloroplasts
Poddar	2021	Advancing enabling technology and genome editing in monocot crops for disease resistance and sustainability
WO2026072580A1 (fr)	2026-04-02	Compositions à base d'eif4e et procédés pour assurer une résistance virale chez le maïs