CA2275885C - Pommes de terre transgeniques avec reduction des niveaux d'activite de la tubercule-phosphorylase de type l ou h alpha-glucan et avec reduction de l'adoucissement au froid - Google Patents

Pommes de terre transgeniques avec reduction des niveaux d'activite de la tubercule-phosphorylase de type l ou h alpha-glucan et avec reduction de l'adoucissement au froid Download PDF

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Publication number
CA2275885C
CA2275885C CA002275885A CA2275885A CA2275885C CA 2275885 C CA2275885 C CA 2275885C CA 002275885 A CA002275885 A CA 002275885A CA 2275885 A CA2275885 A CA 2275885A CA 2275885 C CA2275885 C CA 2275885C
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tubers
glu
leu
ala
lys
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Expired - Fee Related
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CA002275885A
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CA2275885A1 (fr
Inventor
Lawrence Michael Kawchuk
John David Armstrong
Dermot Roborg Lynch
Norman Richard Knowles
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Agriculture and Agri-Food Canada
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Agriculture and Agri-Food Canada
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Priority claimed from US08/868,786 external-priority patent/US5998701A/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Nutrition Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Preparation Of Fruits And Vegetables (AREA)
  • Buildings Adapted To Withstand Abnormal External Influences (AREA)
  • Vibration Prevention Devices (AREA)
  • Vibration Dampers (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Cette invention se rapporte à des cultures de pommes de terre, qui présentent une réduction des niveaux d'activité des enzymes tubercule-phosphorylase de type L alpha -glucan (GLTP) ou tubercule-phosphorylase de type H alpha -glucan (GHTP) dans le tubercule de la pomme de terre. La conversion des amidons en sucres dans les tubercules des pommes de terre, en particulier lorsqu'ils sont conservés à des températures inférieures à 7 DEG C, est réduite dans les tubercules présentant une réduction d'activité des enzymes GLTP ou GHTP. La réduction de l'adoucissement au froid des pommes de terre permet une conservation des pommes de terre à des températures plus froides, ce qui permet d'obtenir une dormance prolongée, une incidence réduite des maladies et une durée de conservation accrue. Cette invention décrit également des procédés pour produire des cultures de pommes de terre qui génèrent des tubercules présentant une réduction d'activité des enzymes GLTP ou GHTP. La réduction de l'activité des GLTP ou GHTP dans le tubercule de la pomme de terre peut être obtenu par des techniques telles que la suppression de l'expression génique, au moyen d'ARN anti-sens homologue, l'utilisation de la co-suppression, de séquences silencieuses régulatoires, d'inhibiteurs chimiques et protéiques et l'utilisation de la mutagenèse dirigée sur site ou de l'isolation d'allèles alternatifs, pour obtenir des variants de GLTP ou de GHTP avec une affinité réduite à l'amidon ou une activité réduite de l'amidon.
CA002275885A 1997-02-10 1998-02-05 Pommes de terre transgeniques avec reduction des niveaux d'activite de la tubercule-phosphorylase de type l ou h alpha-glucan et avec reduction de l'adoucissement au froid Expired - Fee Related CA2275885C (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US3694697P 1997-02-10 1997-02-10
US60/036,946 1997-02-10
US08/868,786 1997-06-04
US08/868,786 US5998701A (en) 1997-06-04 1997-06-04 Potatoes having improved quality characteristics and methods for their production
PCT/CA1998/000055 WO1998035051A1 (fr) 1997-02-10 1998-02-05 Pommes de terre transgeniques avec reduction des niveaux d'activite de la tubercule-phosphorylase de type l ou h alpha-glucan et avec reduction de l'adoucissement au froid

Publications (2)

Publication Number Publication Date
CA2275885A1 CA2275885A1 (fr) 1998-08-13
CA2275885C true CA2275885C (fr) 2007-01-09

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Family Applications (1)

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CA002275885A Expired - Fee Related CA2275885C (fr) 1997-02-10 1998-02-05 Pommes de terre transgeniques avec reduction des niveaux d'activite de la tubercule-phosphorylase de type l ou h alpha-glucan et avec reduction de l'adoucissement au froid

Country Status (11)

Country Link
EP (1) EP1009839A1 (fr)
JP (1) JP2001511007A (fr)
CN (2) CN1246894A (fr)
AR (1) AR011121A1 (fr)
AU (1) AU724942B2 (fr)
BR (1) BR9807214A (fr)
CA (1) CA2275885C (fr)
HU (1) HUP0000542A3 (fr)
NZ (1) NZ336766A (fr)
PL (1) PL334962A1 (fr)
WO (1) WO1998035051A1 (fr)

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AUPO069996A0 (en) 1996-06-27 1996-07-18 Australian National University, The Manipulation of plant cellulose
NZ535395A (en) 2002-02-20 2009-01-31 Simplot Co J R Precise plant breeding using plant DNA border like sequences instead of Agrobacterium borders
US7534934B2 (en) 2002-02-20 2009-05-19 J.R. Simplot Company Precise breeding
US8250818B2 (en) * 2004-03-03 2012-08-28 Robert Tremblay Self-centering energy dissipative brace apparatus with tensioning elements
FR2868080B1 (fr) * 2004-03-29 2007-11-16 Genoplante Valor Soc Par Actio Procede d'amelioration des plantes
RU2331433C2 (ru) * 2004-10-27 2008-08-20 Закрытое акционерное общество научно-производственная фирма "Гемма-Б" (ЗАО НПФ "Гемма-Б") Новый растительный глюкан как иммуноадъювант
JP4654089B2 (ja) * 2004-12-03 2011-03-16 新日本製鐵株式会社 耐久密着性に優れたクロメートフリー樹脂複合型制振材料
CN100398758C (zh) * 2004-12-13 2008-07-02 东南大学 复合减震器
CN100516394C (zh) * 2005-12-02 2009-07-22 卢锐 叠合钢板消能减震阻尼器
DE102007030883A1 (de) 2007-07-03 2009-01-08 Schaeffler Kg Dichtung für eine hydrostatische Linearführung
CN102325884B (zh) * 2009-02-20 2013-11-20 国立大学法人东京大学 低免疫原性链霉抗生物素蛋白及其应用
KR101296702B1 (ko) * 2009-03-27 2013-08-20 무라다기카이가부시끼가이샤 2축 공작 기계
EP4215041A1 (fr) * 2009-10-26 2023-07-26 Agventure B.V. Culture de semences de pomme de terre hybride
FR2957998B1 (fr) * 2010-03-29 2013-04-26 Eurocopter France Amortisseur d'une pale, et rotor muni d'un tel amortisseur
CN102409774B (zh) * 2011-09-02 2013-07-31 北京交通大学 一种防屈曲支撑式建筑用黏弹性阻尼器
CN103216017A (zh) * 2012-01-18 2013-07-24 南京丹普科技工程有限公司 一种粘弹性阻尼器
CN103276828B (zh) * 2013-05-22 2015-08-19 太原理工大学 短接加劲的曲线拉杆式抗拔器
KR101516421B1 (ko) * 2013-08-22 2015-05-26 김시언 감자의 포도당 함량 속성 측정 방법
CN104314192A (zh) * 2014-10-22 2015-01-28 上海大学 一种带位移放大装置的多层阻尼器
CN104453000A (zh) * 2014-10-24 2015-03-25 大连理工大学 一种填充粘弹性材料的复合型防屈曲支撑
CN104534014B (zh) * 2014-11-11 2016-10-12 上海宇航系统工程研究所 一种具有减振性能的剪切式阻尼管及减振适配器
CN104895253B (zh) * 2015-04-20 2017-04-26 北京工业大学 一种阻尼钢筋连接器
CN104879414B (zh) * 2015-04-30 2017-04-05 北京空间机电研究所 一种粘弹性阻尼桁架隔振器
CN105507494B (zh) * 2016-01-15 2018-03-27 兰州理工大学 叠合齿形钢板复合阻尼耗能型可更换连梁及其施工方法
CN106436951B (zh) * 2016-10-18 2018-08-31 中国建筑第八工程局有限公司 咬合式两阶段摩擦耗能阻尼器
CN106481135B (zh) * 2016-11-15 2019-04-12 东南大学 一种端部具有中部凹陷型诱导单元的屈曲诱导支撑
CN106760834B (zh) * 2016-11-15 2019-01-08 东南大学 一种端部具有变长度双层内凹型诱导单元的屈曲诱导支撑
CN107130831B (zh) * 2017-05-25 2019-03-29 东南大学 一种多维波面厚粘弹性层隔减振/震装置
BR112021021149A2 (pt) 2019-05-29 2021-12-14 Monsanto Technology Llc Métodos e composições para a geração de alelos dominantes utilizando a edição de genomas
CN110528720B (zh) * 2019-09-20 2024-05-17 广州大学 一种金属复合型阻尼器
CN115045947A (zh) * 2022-06-09 2022-09-13 北京金茂人居环境科技有限公司 轴向磁流变阻尼宽频隔振器
LU502613B1 (en) * 2022-08-01 2024-02-01 Plant Bioscience Ltd Methods of altering the starch granule profile in plants

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DK198089D0 (da) * 1989-04-24 1989-04-24 Danske Spritfabrikker Dna-materialer og anvendelse deraf
DE4220758A1 (de) * 1992-06-24 1994-01-05 Inst Genbiologische Forschung DNA-Sequenz und Plasmide zur Herstellung von Pflanzen mit veränderter Saccharose-Konzentration
US5648249A (en) * 1993-05-28 1997-07-15 Monsato Company Method of improving the quality of stored potatoes
CN1137997C (zh) * 1995-12-27 2004-02-11 日本烟草产业株式会社 低温诱导性启动子序列

Also Published As

Publication number Publication date
HUP0000542A3 (en) 2002-02-28
AR011121A1 (es) 2000-08-02
CA2275885A1 (fr) 1998-08-13
CN1246894A (zh) 2000-03-08
NZ336766A (en) 2000-08-25
AU724942B2 (en) 2000-10-05
CN1113143C (zh) 2003-07-02
JP2001511007A (ja) 2001-08-07
PL334962A1 (en) 2000-03-27
BR9807214A (pt) 2000-04-25
EP1009839A1 (fr) 2000-06-21
AU5849398A (en) 1998-08-26
CN1300338A (zh) 2001-06-20
HUP0000542A2 (hu) 2000-06-28
WO1998035051A1 (fr) 1998-08-13

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