CA2275974C - Procedes permettant de produire des plantes transgeniques femelles steriles - Google Patents

Procedes permettant de produire des plantes transgeniques femelles steriles Download PDF

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Publication number
CA2275974C
CA2275974C CA002275974A CA2275974A CA2275974C CA 2275974 C CA2275974 C CA 2275974C CA 002275974 A CA002275974 A CA 002275974A CA 2275974 A CA2275974 A CA 2275974A CA 2275974 C CA2275974 C CA 2275974C
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CA
Canada
Prior art keywords
nucleic acid
sequence
seq
promoter
plants
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA002275974A
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English (en)
Other versions
CA2275974A1 (fr
Inventor
Heinz Saedler
Hans Sommer
Giuseppe Leonardo Rotino
Angelo Spena
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Phytowelt GreenTechnologies GmbH
CRA - UNITA DI RICERCA PER I'ORTICOLTURA (MONTANASO LOMBARDO LO)
Original Assignee
Phytowelt GreenTechnologies GmbH
ISTITUTO SPERIMENTALE PER L'ORTICOLTURA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Phytowelt GreenTechnologies GmbH, ISTITUTO SPERIMENTALE PER L'ORTICOLTURA filed Critical Phytowelt GreenTechnologies GmbH
Publication of CA2275974A1 publication Critical patent/CA2275974A1/fr
Application granted granted Critical
Publication of CA2275974C publication Critical patent/CA2275974C/fr
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8233—Female-specific, e.g. pistil, ovule
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
    • C12N15/829—Female sterility
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8291—Hormone-influenced development
    • C12N15/8294—Auxins

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Reproductive Health (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Endocrinology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Cosmetics (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • External Artificial Organs (AREA)
  • Mushroom Cultivation (AREA)

Abstract

L'invention concerne l'utilisation de la région du promoteur du gène DefH9 d'Anthirrhinum majus ou d'un promoteur d'un gène homologue présentant le même motif d'expression et les mêmes caractéristiques, en vue de l'établissement d'une parthénocarpie ou d'une stérilité de plantes femelles dans le but d'accroître une gynogenèse ou pour améliorer la nouaison et le développement de fruits. Des molécules d'ADN recombinées sont également décrites, dont notamment un promoteur de DefH9 en combinaison avec une séquence d'ADN qui, après expression dans des plantes, produit les effets mentionnés précédemment. De plus, des cellules de plantes et des plantes transformées à l'aide de telles molécules d'ADN recombinées sont décrites.
CA002275974A 1996-12-20 1997-12-19 Procedes permettant de produire des plantes transgeniques femelles steriles Expired - Fee Related CA2275974C (fr)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
EP96120645 1996-12-20
EP96120645.5 1996-12-20
EP97104156 1997-03-12
EP97104156.1 1997-03-12
EP97105843 1997-04-09
EP97105843.3 1997-04-09
PCT/EP1997/007202 WO1998028430A1 (fr) 1996-12-20 1997-12-19 Procedes permettant de produire des plantes transgeniques femelles steriles

Publications (2)

Publication Number Publication Date
CA2275974A1 CA2275974A1 (fr) 1998-07-02
CA2275974C true CA2275974C (fr) 2008-05-27

Family

ID=27237479

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002275974A Expired - Fee Related CA2275974C (fr) 1996-12-20 1997-12-19 Procedes permettant de produire des plantes transgeniques femelles steriles

Country Status (12)

Country Link
US (1) US6483012B1 (fr)
EP (1) EP0951558B1 (fr)
JP (1) JP2001506857A (fr)
AT (1) ATE291093T1 (fr)
AU (1) AU728015C (fr)
BR (1) BR9714425A (fr)
CA (1) CA2275974C (fr)
DE (1) DE69732792D1 (fr)
IL (1) IL130457A0 (fr)
NZ (1) NZ336555A (fr)
TR (2) TR200000858T2 (fr)
WO (1) WO1998028430A1 (fr)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IT1306169B1 (it) * 1999-07-16 2001-05-30 G In E St R A Soc Consortile A Metodo per modulare l'espressione di geni che inducono il caratterepartenocarpia in piante.
AU7614700A (en) * 1999-09-20 2001-04-24 Cambia Megagametophyte transcriptional control elements and uses thereof
AU2001237421B2 (en) 2000-03-02 2006-06-22 Limagrain Europe Embryo sac-specific genes
ATE530654T1 (de) 2001-07-06 2011-11-15 Monsanto Technology Llc Verfahren zur förderung der aufspaltung von transgenen bei pflanzen und zusammensetzungen dafür
US20040016016A1 (en) * 2002-06-19 2004-01-22 Mankin S. Luke Compositions and methods for improving plant performance
WO2004108903A2 (fr) * 2003-06-06 2004-12-16 Arborgen, Llc Transformation et selection de vegetaux
CN101023175B (zh) * 2004-04-20 2012-02-29 辛根塔参与股份公司 用于在植物繁殖组织中表达基因产物的调控序列
JP5330231B2 (ja) 2006-05-12 2013-10-30 モンサント テクノロジー エルエルシー マーカーフリーのトランスジェニック植物を得るための方法および組成物
CA2615249C (fr) 2006-12-01 2014-02-18 Pontificia Universidad Catolica De Chile Methode de production de fleurs males steriles et de fruits parthenocarpiques au moyen d'extinction de genes, sequences associees et vecteurs contenant ces sequences
IL183902A0 (en) * 2007-06-13 2007-10-31 Zeraim Gedera Ltd Parthenocarpic pepper
US8178756B2 (en) 2009-02-05 2012-05-15 Curry Seed & Chili Co. Thin-skinned chile pepper variety
BR112012009044A2 (pt) * 2009-10-26 2015-09-01 Pioneer Hi Bred Int Molécula de ácido nucléico isolada, cassete de expressão, vetor, célula vegetal, planta, semente transgênica, método para expressão de um polinucleotídeo em uma planta ou célula vegetal e método para expressão de um polinucleotídeo, preferencialmente em tecidos somáticos de óvulo de uma planta
CA2860611A1 (fr) * 2012-01-06 2013-07-11 Pioneer Hi-Bred International, Inc. Compositions et procedes d'expression de sequence dans un tissu de reproduction de plante
EP3079460B1 (fr) 2013-12-12 2026-05-06 Rijk Zwaan Zaadteelt en Zaadhandel B.V. Formation de fruit indépendante de la fertilisation dans l'aubergine

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4943674A (en) * 1987-05-26 1990-07-24 Calgene, Inc. Fruit specific transcriptional factors
US6268546B1 (en) * 1989-07-19 2001-07-31 Calgene Llc Ovary-tissue transcriptional factors
EP0412006B1 (fr) * 1989-08-04 2000-11-29 Aventis CropScience N.V. Plantes à fleurs, semences ou embryons modifiés
IL117139A0 (en) 1996-02-14 1996-06-18 Volcani Center Method for the induction of genetic parthenocarpy in plants
US5877400A (en) 1996-05-01 1999-03-02 Pioneer Hi-Bred International, Inc. Transgenic methods and compositions for producing parthenocarpic fruits and vegetables

Also Published As

Publication number Publication date
ATE291093T1 (de) 2005-04-15
DE69732792D1 (de) 2005-04-21
AU728015C (en) 2002-02-21
JP2001506857A (ja) 2001-05-29
IL130457A0 (en) 2000-06-01
EP0951558B1 (fr) 2005-03-16
NZ336555A (en) 2000-12-22
BR9714425A (pt) 2000-04-25
AU728015B2 (en) 2001-01-04
TR199901418T2 (xx) 1999-10-21
US6483012B1 (en) 2002-11-19
AU5762398A (en) 1998-07-17
WO1998028430A1 (fr) 1998-07-02
CA2275974A1 (fr) 1998-07-02
TR200000858T2 (tr) 2000-07-21
EP0951558A1 (fr) 1999-10-27

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