CA2280196C - Genes chimeres et procede permettant d'accroitre la teneur en lysine dans les graines de vegetaux - Google Patents

Genes chimeres et procede permettant d'accroitre la teneur en lysine dans les graines de vegetaux Download PDF

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Publication number
CA2280196C
CA2280196C CA2280196A CA2280196A CA2280196C CA 2280196 C CA2280196 C CA 2280196C CA 2280196 A CA2280196 A CA 2280196A CA 2280196 A CA2280196 A CA 2280196A CA 2280196 C CA2280196 C CA 2280196C
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Canada
Prior art keywords
lysine
gene
seq
seeds
protein
Prior art date
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Expired - Fee Related
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CA2280196A
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English (en)
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CA2280196A1 (fr
Inventor
Saverio Carl Falco
Raymond Ervin Mcdevitt Iii
Sabine Ursula Epelbaum
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EIDP Inc
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EI Du Pont de Nemours and Co
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Publication of CA2280196A1 publication Critical patent/CA2280196A1/fr
Application granted granted Critical
Publication of CA2280196C publication Critical patent/CA2280196C/fr
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8234—Seed-specific, e.g. embryo, endosperm
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C12N15/8254—Tryptophan or lysine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Nutrition Science (AREA)
  • Reproductive Health (AREA)
  • Pregnancy & Childbirth (AREA)
  • Developmental Biology & Embryology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Pretreatment Of Seeds And Plants (AREA)

Abstract

L'invention porte sur des gènes chimères, un gène chimère codant une cétoglutarate réductase de la lysine et un second gène chimère codant une synthase d'acide dihydrodipicolinique insensible à la lysine et qui est lié de manière fonctionnelle à une séquence de transit de chloroplaste de végétaux, ces gènes étant liés de manière fonctionnelle à des séquences régulatrices spécifiques des graines de végétaux. L'invention porte également sur les procédés d'utilisation de ces gènes en vue de produire des taux supérieurs de lysine dans les graines de végétaux transformés.
CA2280196A 1997-03-27 1998-03-27 Genes chimeres et procede permettant d'accroitre la teneur en lysine dans les graines de vegetaux Expired - Fee Related CA2280196C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US82462797A 1997-03-27 1997-03-27
US08/824,627 1997-03-27
PCT/US1998/006051 WO1998042831A2 (fr) 1997-03-27 1998-03-27 Genes chimeres et procede permettant d'accroitre la teneur en lysine dans les graines de vegetaux

Publications (2)

Publication Number Publication Date
CA2280196A1 CA2280196A1 (fr) 1998-10-01
CA2280196C true CA2280196C (fr) 2012-05-15

Family

ID=25241893

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2280196A Expired - Fee Related CA2280196C (fr) 1997-03-27 1998-03-27 Genes chimeres et procede permettant d'accroitre la teneur en lysine dans les graines de vegetaux

Country Status (12)

Country Link
EP (1) EP0973880A2 (fr)
JP (1) JP2001502923A (fr)
KR (1) KR20010005645A (fr)
CN (1) CN1253584A (fr)
AU (1) AU747997B2 (fr)
BR (1) BR9811256A (fr)
CA (1) CA2280196C (fr)
HU (1) HUP0002305A3 (fr)
ID (1) ID22486A (fr)
PL (1) PL336042A1 (fr)
TR (1) TR199902349T2 (fr)
WO (1) WO1998042831A2 (fr)

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Also Published As

Publication number Publication date
HUP0002305A3 (en) 2002-09-30
AU6780198A (en) 1998-10-20
HUP0002305A2 (hu) 2000-09-28
WO1998042831A3 (fr) 1998-12-10
EP0973880A2 (fr) 2000-01-26
WO1998042831A2 (fr) 1998-10-01
BR9811256A (pt) 2000-07-18
ID22486A (id) 1999-10-21
AU747997B2 (en) 2002-05-30
CA2280196A1 (fr) 1998-10-01
JP2001502923A (ja) 2001-03-06
TR199902349T2 (xx) 2000-01-21
KR20010005645A (ko) 2001-01-15
CN1253584A (zh) 2000-05-17
PL336042A1 (en) 2000-06-05

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