CA2280196C - Genes chimeres et procede permettant d'accroitre la teneur en lysine dans les graines de vegetaux - Google Patents

Genes chimeres et procede permettant d'accroitre la teneur en lysine dans les graines de vegetaux Download PDF

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Publication number
CA2280196C
CA2280196C CA2280196A CA2280196A CA2280196C CA 2280196 C CA2280196 C CA 2280196C CA 2280196 A CA2280196 A CA 2280196A CA 2280196 A CA2280196 A CA 2280196A CA 2280196 C CA2280196 C CA 2280196C
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CA
Canada
Prior art keywords
lysine
gene
seq
seeds
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
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CA2280196A
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English (en)
Other versions
CA2280196A1 (fr
Inventor
Saverio Carl Falco
Raymond Ervin Mcdevitt Iii
Sabine Ursula Epelbaum
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EIDP Inc
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EI Du Pont de Nemours and Co
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Publication of CA2280196A1 publication Critical patent/CA2280196A1/fr
Application granted granted Critical
Publication of CA2280196C publication Critical patent/CA2280196C/fr
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8234—Seed-specific, e.g. embryo, endosperm
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C12N15/8254—Tryptophan or lysine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Nutrition Science (AREA)
  • Reproductive Health (AREA)
  • Pregnancy & Childbirth (AREA)
  • Developmental Biology & Embryology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Pretreatment Of Seeds And Plants (AREA)

Abstract

L'invention porte sur des gènes chimères, un gène chimère codant une cétoglutarate réductase de la lysine et un second gène chimère codant une synthase d'acide dihydrodipicolinique insensible à la lysine et qui est lié de manière fonctionnelle à une séquence de transit de chloroplaste de végétaux, ces gènes étant liés de manière fonctionnelle à des séquences régulatrices spécifiques des graines de végétaux. L'invention porte également sur les procédés d'utilisation de ces gènes en vue de produire des taux supérieurs de lysine dans les graines de végétaux transformés.
CA2280196A 1997-03-27 1998-03-27 Genes chimeres et procede permettant d'accroitre la teneur en lysine dans les graines de vegetaux Expired - Fee Related CA2280196C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US82462797A 1997-03-27 1997-03-27
US08/824,627 1997-03-27
PCT/US1998/006051 WO1998042831A2 (fr) 1997-03-27 1998-03-27 Genes chimeres et procede permettant d'accroitre la teneur en lysine dans les graines de vegetaux

Publications (2)

Publication Number Publication Date
CA2280196A1 CA2280196A1 (fr) 1998-10-01
CA2280196C true CA2280196C (fr) 2012-05-15

Family

ID=25241893

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2280196A Expired - Fee Related CA2280196C (fr) 1997-03-27 1998-03-27 Genes chimeres et procede permettant d'accroitre la teneur en lysine dans les graines de vegetaux

Country Status (12)

Country Link
EP (1) EP0973880A2 (fr)
JP (1) JP2001502923A (fr)
KR (1) KR20010005645A (fr)
CN (1) CN1253584A (fr)
AU (1) AU747997B2 (fr)
BR (1) BR9811256A (fr)
CA (1) CA2280196C (fr)
HU (1) HUP0002305A3 (fr)
ID (1) ID22486A (fr)
PL (1) PL336042A1 (fr)
TR (1) TR199902349T2 (fr)
WO (1) WO1998042831A2 (fr)

Families Citing this family (66)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1074629A1 (fr) * 1999-07-30 2001-02-07 Coöperatieve Verkoop- en Productievereniging van Aardappelmeel en Derivaten 'AVEBE' B.A. Protéines pièges
US7157281B2 (en) 2003-12-11 2007-01-02 Monsanto Technology Llc High lysine maize compositions and event LY038 maize plants
AR047598A1 (es) * 2004-02-10 2006-01-25 Monsanto Technology Llc Semilla de maiz transgenica con mayor contenido de aminoacidos
US7855323B2 (en) 2004-02-10 2010-12-21 Monsanto Technology Llc Recombinant DNA for gene suppression
RU2460281C2 (ru) * 2005-10-03 2012-09-10 Монсанто Текнолоджи Ллс Семена трансгенных растений с повышенным содержанием лизина
CN100412198C (zh) * 2006-04-05 2008-08-20 北京凯拓迪恩生物技术研发中心有限责任公司 一种提高水稻种子中赖氨酸含量的方法及其专用载体
US7947877B2 (en) 2008-05-14 2011-05-24 Monosanto Technology LLC Plants and seeds of spring canola variety SCV328921
US7964774B2 (en) 2008-05-14 2011-06-21 Monsanto Technology Llc Plants and seeds of spring canola variety SCV384196
US8829282B2 (en) 2008-05-14 2014-09-09 Monsanto Technology, Llc Plants and seeds of spring canola variety SCV425044
US7935870B2 (en) 2008-05-14 2011-05-03 Monsanto Technology Llc Plants and seeds of spring canola variety SCV354718
MX2011013224A (es) 2009-06-09 2012-06-01 Pioneer Hi Bred Int Promotor de endospermo temprano y metodos de uso.
US8071848B2 (en) 2009-06-17 2011-12-06 Monsanto Technology Llc Plants and seeds of spring canola variety SCV218328
BR112012009044A2 (pt) 2009-10-26 2015-09-01 Pioneer Hi Bred Int Molécula de ácido nucléico isolada, cassete de expressão, vetor, célula vegetal, planta, semente transgênica, método para expressão de um polinucleotídeo em uma planta ou célula vegetal e método para expressão de um polinucleotídeo, preferencialmente em tecidos somáticos de óvulo de uma planta
CN102051376B (zh) * 2010-01-27 2013-03-20 华中农业大学 基于油菜子叶叶绿体转化的组织培养体系及获得转化植株的方法
US8148611B2 (en) 2010-02-26 2012-04-03 Monsanto Technology Llc Plants and seeds of spring canola variety SCV453784
US8143488B2 (en) 2010-02-26 2012-03-27 Monsanto Technoloy LLC Plants and seeds of spring canola variety SCV470336
US8138394B2 (en) 2010-02-26 2012-03-20 Monsanto Technology Llc Plants and seeds of spring canola variety SCV431158
US8581048B2 (en) 2010-03-09 2013-11-12 Monsanto Technology, Llc Plants and seeds of spring canola variety SCV119103
US8153865B2 (en) 2010-03-11 2012-04-10 Monsanto Technology Llc Plants and seeds of spring canola variety SCV152154
US8513487B2 (en) 2011-04-07 2013-08-20 Zenon LISIECZKO Plants and seeds of spring canola variety ND-662c
US8513494B2 (en) 2011-04-08 2013-08-20 Chunren Wu Plants and seeds of spring canola variety SCV695971
US8507761B2 (en) 2011-05-05 2013-08-13 Teresa Huskowska Plants and seeds of spring canola variety SCV372145
US8513495B2 (en) 2011-05-10 2013-08-20 Dale Burns Plants and seeds of spring canola variety SCV291489
US20130167262A1 (en) 2011-12-21 2013-06-27 The Curators Of The University Of Missouri Soybean variety s05-11268
US9204603B2 (en) 2011-12-21 2015-12-08 The Curators Of The University Of Missouri Soybean variety S05-11482
WO2013103365A1 (fr) 2012-01-06 2013-07-11 Pioneer Hi-Bred International, Inc. Promoteurs de pollen préférés et procédés d'utilisation
EP2800816A1 (fr) 2012-01-06 2014-11-12 Pioneer Hi-Bred International Inc. Promoteur spécifique d'ovule et méthodes d'utilisation
US8835720B2 (en) 2012-04-26 2014-09-16 Monsanto Technology Llc Plants and seeds of spring canola variety SCV967592
US8802935B2 (en) 2012-04-26 2014-08-12 Monsanto Technology Llc Plants and seeds of spring canola variety SCV942568
US8859857B2 (en) 2012-04-26 2014-10-14 Monsanto Technology Llc Plants and seeds of spring canola variety SCV259778
US8878009B2 (en) 2012-04-26 2014-11-04 Monsanto Technology, LLP Plants and seeds of spring canola variety SCV318181
WO2014059155A1 (fr) 2012-10-11 2014-04-17 Pioneer Hi-Bred International, Inc. Promoteurs de cellules de garde et leur utilisation
EA028528B1 (ru) 2013-03-13 2017-11-30 Пайонир Хай-Бред Интернэшнл, Инк. Внесение глифосата для борьбы с сорняками у brassica
WO2014153254A2 (fr) 2013-03-14 2014-09-25 Pioneer Hi-Bred International Inc. Compositions et procédés pour contrôler des insectes ravageurs
CA2901316A1 (fr) 2013-03-15 2014-09-25 Pioneer Hi-Bred International, Inc. Polypeptides phi-4 et leurs procedes d'utilisation
BR112016003225B1 (pt) 2013-08-16 2022-10-25 Pioneer Hi-Bred International, Inc. Polipeptídeo pip-47, polipeptídeo pip-47 quimérico, composição, proteína de fusão, método para controlar uma população de inseto-praga, método para inibir o crescimento ou matar um inseto-praga, construto de dna, polinucleotídeo isolado, cassete de expressão, método de obtenção de uma planta transgênica e método para controlar infestação de inseto
BR122020001770B1 (pt) 2013-09-13 2022-11-29 Pioneer Hi-Bred International, Inc Construto de dna, método de obtenção de planta transgênica, proteína de fusão, método para controlar uma população de praga de inseto, método para inibir o crescimento ou matar uma praga de inseto
CN106029870A (zh) 2014-01-16 2016-10-12 凯利斯塔公司 用于增强的氨基酸产生的微生物及相关方法
CN106536545B (zh) 2014-02-07 2026-03-03 先锋国际良种公司 杀昆虫蛋白及其使用方法
CA2955828A1 (fr) 2014-08-08 2016-02-11 Pioneer Hi-Bred International, Inc. Promoteurs d'ubiquitine et introns, et procedes d'utilisation
WO2016044092A1 (fr) 2014-09-17 2016-03-24 Pioneer Hi Bred International Inc Compositions et procédés de lutte contre des insectes ravageurs
CN113372421B (zh) 2014-10-16 2024-08-06 先锋国际良种公司 杀昆虫蛋白及其使用方法
US20170359965A1 (en) 2014-12-19 2017-12-21 E I Du Pont De Nemours And Company Polylactic acid compositions with accelerated degradation rate and increased heat stability
CN114075267B (zh) 2015-01-15 2025-03-18 先锋国际良种公司 杀昆虫蛋白及其使用方法
CA2985198A1 (fr) 2015-05-19 2016-11-24 Pioneer Hi-Bred International, Inc. Proteines insecticides et leurs procedes d'utilisation
EP3310803A1 (fr) 2015-06-16 2018-04-25 Pioneer Hi-Bred International, Inc. Compositions et procédés de lutte contre des insectes nuisibles
BR112018002535A2 (pt) 2015-08-06 2018-09-25 Du Pont polipeptídeo inseticida recombinante, polinucleotídeo recombinante, construto de dna, planta transgênica ou célula de planta, composição, proteína de fusão, método para controlar uma praga, método para inibir crescimento ou para exterminar uma população de pragas ou uma praga e uso do polipeptídeo
DK3341483T3 (da) 2015-08-28 2020-03-16 Pioneer Hi Bred Int Ochrobactrum-medieret transformation af planter
CN108575091A (zh) 2015-12-18 2018-09-25 先锋国际良种公司 杀昆虫蛋白及其使用方法
CA3004056C (fr) 2015-12-22 2024-01-23 Pioneer Hi-Bred International, Inc. Promoteurs preferes par un tissu et procedes d'utilisation
CA3018384A1 (fr) 2016-05-04 2017-11-09 Pioneer Hi-Bred International, Inc. Proteines insecticides et procedes pour les utiliser
CA3022858A1 (fr) 2016-06-16 2017-12-21 Pioneer Hi-Bred International, Inc. Compositions et procedes de lutte contre des insectes nuisibles
UA127388C2 (uk) 2016-06-24 2023-08-09 Піонір Хай-Бред Інтернешнл, Інк. Регуляторний елемент рослини і спосіб його застосування
EP3478052B1 (fr) 2016-07-01 2021-08-25 Pioneer Hi-Bred International, Inc. Protéines insecticides issues de plantes et procédés pour leur utilisation
WO2018013333A1 (fr) 2016-07-12 2018-01-18 Pioneer Hi-Bred International, Inc. Compositions et procédés de lutte contre des insectes nuisibles
EP3535285B1 (fr) 2016-11-01 2022-04-06 Pioneer Hi-Bred International, Inc. Protéines insecticides et leurs procédés d'utilisation
CN111373046A (zh) 2017-09-25 2020-07-03 先锋国际良种公司 组织偏好性启动子和使用方法
US11332752B2 (en) 2018-03-12 2022-05-17 Pioneer Hi-Bred International, Inc. Use of morphogenic factors for the improvement of gene editing
CA3092078A1 (fr) 2018-03-14 2019-09-19 Pioneer Hi-Bred International, Inc. Proteines insecticides issues de plantes et procedes pour leur utilisation
AU2019234562B2 (en) 2018-03-14 2024-08-01 Pioneer Hi-Bred International, Inc. Insecticidal proteins from plants and methods for their use
WO2019226508A1 (fr) 2018-05-22 2019-11-28 Pioneer Hi-Bred International, Inc. Éléments régulateurs de plante et leurs procédés d'utilisation
CA3097915A1 (fr) 2018-06-28 2020-01-02 Pioneer Hi-Bred International, Inc. Procedes de selection de plantes transformees
US20210395758A1 (en) 2018-10-31 2021-12-23 Pioneer Hi-Bred International, Inc. Compositions and methods for ochrobactrum-mediated plant transformation
BR112022027035A2 (pt) 2020-07-14 2023-04-11 Pioneer Hi Bred Int Proteínas inseticidas e métodos para uso das mesmas
CN114317557B (zh) * 2022-01-06 2023-07-07 河南农业大学 玉米ZmRIBA1基因在高赖氨酸玉米育种中的应用
EP4725303A1 (fr) 2024-10-11 2026-04-15 Agrokray Limited Liability Company Procede de production de plantes sphellantes, grain produit a partir de plantes spellantes et produit alimentaire produit a partir de celles-ci

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5258300A (en) * 1988-06-09 1993-11-02 Molecular Genetics Research And Development Limited Partnership Method of inducing lysine overproduction in plants
DK1394257T3 (da) * 1992-03-19 2007-12-10 Du Pont Nukleinsyrefragmenter og fremgangsmåder til forögelse af lysin- og threonin-indholdet i fröene fra planter
JPH09511124A (ja) * 1993-11-30 1997-11-11 イー・アイ・デユポン・ドウ・ヌムール・アンド・カンパニー キメラ遺伝子ならびにトウモロコシ、ダイズおよびナタネ植物
WO1995034672A1 (fr) * 1994-06-14 1995-12-21 Ajinomoto Co., Inc. GENE A DESHYDROGENASE α-CETOGLUTARIQUE
US5919617A (en) * 1994-12-21 1999-07-06 Miami University Methods and reagents for detecting fungal pathogens in a biological sample

Also Published As

Publication number Publication date
JP2001502923A (ja) 2001-03-06
KR20010005645A (ko) 2001-01-15
CA2280196A1 (fr) 1998-10-01
WO1998042831A3 (fr) 1998-12-10
EP0973880A2 (fr) 2000-01-26
AU6780198A (en) 1998-10-20
TR199902349T2 (xx) 2000-01-21
CN1253584A (zh) 2000-05-17
HUP0002305A2 (hu) 2000-09-28
AU747997B2 (en) 2002-05-30
PL336042A1 (en) 2000-06-05
WO1998042831A2 (fr) 1998-10-01
HUP0002305A3 (en) 2002-09-30
ID22486A (id) 1999-10-21
BR9811256A (pt) 2000-07-18

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