CA2286739A1 - Methodes ameliorees de transport utilisant des lipides cationiques et des lipides assistants - Google Patents

Methodes ameliorees de transport utilisant des lipides cationiques et des lipides assistants Download PDF

Info

Publication number
CA2286739A1
CA2286739A1 CA002286739A CA2286739A CA2286739A1 CA 2286739 A1 CA2286739 A1 CA 2286739A1 CA 002286739 A CA002286739 A CA 002286739A CA 2286739 A CA2286739 A CA 2286739A CA 2286739 A1 CA2286739 A1 CA 2286739A1
Authority
CA
Canada
Prior art keywords
lipid
glycero
phosphoethanolamine
dilauroyl
cationic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002286739A
Other languages
English (en)
Inventor
Jinkang Wang
Yi-Lin Zhang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Urigen Pharmaceuticals Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2286739A1 publication Critical patent/CA2286739A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/88—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microencapsulation, e.g. using amphiphile liposome vesicle
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/54—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic compound
    • A61K47/543—Lipids, e.g. triglycerides; Polyamines, e.g. spermine or spermidine
    • A61K47/544—Phospholipids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/10—Dispersions; Emulsions
    • A61K9/127—Synthetic bilayered vehicles, e.g. liposomes or liposomes with cholesterol as the only non-phosphatidyl surfactant
    • A61K9/1271—Non-conventional liposomes, e.g. PEGylated liposomes or liposomes coated or grafted with polymers
    • A61K9/1272—Non-conventional liposomes, e.g. PEGylated liposomes or liposomes coated or grafted with polymers comprising non-phosphatidyl surfactants as bilayer-forming substances, e.g. cationic lipids or non-phosphatidyl liposomes coated or grafted with polymers

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Dispersion Chemistry (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Medicinal Preparation (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

L'invention concerne des méthodes et des compositions pouvant être utilisées pour introduire des molécules polyanioniques, en particulier des acides nucléiques, dans des cellules mammaliennes, en utilisant certains phosphatidyl éthanolamines comme lipides assistants conjointement avec divers lipides cationiques. En particulier, on améliore la transfection de cellules mammaliennes induite par des lipides cationiques en utilisant des supports lipidiques comprenant 1,2-dilauroyl-sn-glycéro-3-phosphoéthanolamine (DLPE) ou 1,2-diphytanoyl-sn-glycéro-3-phosphoéthanolamine (DiPPE) et des lipides cationiques.
CA002286739A 1997-04-04 1998-04-03 Methodes ameliorees de transport utilisant des lipides cationiques et des lipides assistants Abandoned CA2286739A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US83274997A 1997-04-04 1997-04-04
US08/832,749 1997-04-04
PCT/US1998/006606 WO1998044909A1 (fr) 1997-04-04 1998-04-03 Methodes ameliorees de transport utilisant des lipides cationiques et des lipides assistants

Publications (1)

Publication Number Publication Date
CA2286739A1 true CA2286739A1 (fr) 1998-10-15

Family

ID=25262531

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002286739A Abandoned CA2286739A1 (fr) 1997-04-04 1998-04-03 Methodes ameliorees de transport utilisant des lipides cationiques et des lipides assistants

Country Status (4)

Country Link
EP (1) EP0975332A1 (fr)
AU (1) AU6949098A (fr)
CA (1) CA2286739A1 (fr)
WO (1) WO1998044909A1 (fr)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AUPQ259399A0 (en) 1999-09-01 1999-09-23 Lustre Investments Pte Ltd Therapeutic agents
WO2002089770A2 (fr) * 2001-05-10 2002-11-14 Kuhs Gmbh & Co. Kg Composition pharmaceutique
SI1771206T1 (en) 2004-05-05 2018-06-29 Silence Therapeutics Gmbh Lipids, lipid complexes and use thereof
CN101287497B (zh) 2004-12-27 2013-03-06 赛伦斯治疗公司 涂层脂质复合体和它们的用途
AU2007276388A1 (en) 2006-07-21 2008-01-24 Silence Therapeutics Ag Means for inhibiting the expression of protein kinase 3

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5279833A (en) * 1990-04-04 1994-01-18 Yale University Liposomal transfection of nucleic acids into animal cells
JPH069365A (ja) * 1992-06-25 1994-01-18 Kanebo Ltd 皮膚化粧料
US5650096A (en) * 1994-12-09 1997-07-22 Genzyme Corporation Cationic amphiphiles for intracellular delivery of therapeutic molecules
JPH09248182A (ja) * 1996-03-15 1997-09-22 Oyo Seikagaku Kenkyusho プラスミド包埋多重膜リポソーム
US5935936A (en) * 1996-06-03 1999-08-10 Genzyme Corporation Compositions comprising cationic amphiphiles and co-lipids for intracellular delivery of therapeutic molecules

Also Published As

Publication number Publication date
EP0975332A1 (fr) 2000-02-02
AU6949098A (en) 1998-10-30
WO1998044909A1 (fr) 1998-10-15

Similar Documents

Publication Publication Date Title
US6235310B1 (en) Methods of delivery using cationic lipids and helper lipids
US6187760B1 (en) Cationic phospholipids for transfection
AU754563B2 (en) Cationic lipid formulation delivering nucleic acid to peritoneal tumors
Maurer et al. Lipid-based systems for the intracellular delivery of genetic drugs
US6008202A (en) Stable lipid-comprising drug delivery complexes and methods for their production
Hug et al. Liposomes for the transformation of eukaryotic cells
Zabner Cationic lipids used in gene transfer
US7189705B2 (en) Methods of enhancing SPLP-mediated transfection using endosomal membrane destabilizers
US7993672B2 (en) Stable lipid-comprising drug delivery complexes and methods for their production
US6303378B1 (en) Methods for preparing polynucleotide transfection complexes
US5958894A (en) Amphiphilic biguanide derivatives
Singhal et al. Gene transfer in mammalian cells using liposomes as carriers
CA2286739A1 (fr) Methodes ameliorees de transport utilisant des lipides cationiques et des lipides assistants
WO1998044909A9 (fr) Methodes ameliorees de transport utilisant des lipides cationiques et des lipides assistants
CN120615011A (zh) 脂质组合物及用于递送到免疫细胞的方法
WO1999015206A1 (fr) Procedes de preparation de complexes de transfection lipidiques/polynucleotidiques
Audrezet et al. Novel cationic phosphonolipids agents for in vitro gene transfer to epithelial cell lines

Legal Events

Date Code Title Description
FZDE Dead