CA2304945A1 - Dispositif et procede d'amplification et de dosage d'adn - Google Patents
Dispositif et procede d'amplification et de dosage d'adn Download PDFInfo
- Publication number
- CA2304945A1 CA2304945A1 CA002304945A CA2304945A CA2304945A1 CA 2304945 A1 CA2304945 A1 CA 2304945A1 CA 002304945 A CA002304945 A CA 002304945A CA 2304945 A CA2304945 A CA 2304945A CA 2304945 A1 CA2304945 A1 CA 2304945A1
- Authority
- CA
- Canada
- Prior art keywords
- sample
- dna
- assay
- liquid biological
- card
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 238000000034 method Methods 0.000 title claims description 23
- 238000003556 assay Methods 0.000 title abstract description 75
- 230000004544 DNA amplification Effects 0.000 title abstract description 47
- 239000000523 sample Substances 0.000 claims abstract description 165
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 56
- 230000003321 amplification Effects 0.000 claims abstract description 32
- 238000003199 nucleic acid amplification method Methods 0.000 claims abstract description 32
- 238000006243 chemical reaction Methods 0.000 claims abstract description 29
- 239000007788 liquid Substances 0.000 claims description 73
- 239000012472 biological sample Substances 0.000 claims description 72
- 108020004707 nucleic acids Proteins 0.000 claims description 25
- 150000007523 nucleic acids Chemical class 0.000 claims description 25
- 102000039446 nucleic acids Human genes 0.000 claims description 25
- 238000012546 transfer Methods 0.000 claims description 10
- 238000011067 equilibration Methods 0.000 claims description 4
- 239000003298 DNA probe Substances 0.000 abstract description 34
- 238000002875 fluorescence polarization Methods 0.000 abstract description 31
- 108020003215 DNA Probes Proteins 0.000 abstract description 30
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- 238000007826 nucleic acid assay Methods 0.000 description 6
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- 230000005540 biological transmission Effects 0.000 description 3
- 238000010276 construction Methods 0.000 description 3
- 239000012530 fluid Substances 0.000 description 3
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- 238000011109 contamination Methods 0.000 description 2
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- 239000002985 plastic film Substances 0.000 description 2
- 229920006255 plastic film Polymers 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000006276 transfer reaction Methods 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 229920006358 Fluon Polymers 0.000 description 1
- 101100059652 Mus musculus Cetn1 gene Proteins 0.000 description 1
- 101100059655 Mus musculus Cetn2 gene Proteins 0.000 description 1
- 108020004711 Nucleic Acid Probes Proteins 0.000 description 1
- 239000002390 adhesive tape Substances 0.000 description 1
- 230000031018 biological processes and functions Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
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- 239000011159 matrix material Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000002853 nucleic acid probe Substances 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
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- 238000003856 thermoforming Methods 0.000 description 1
- ILJSQTXMGCGYMG-UHFFFAOYSA-N triacetic acid Chemical compound CC(=O)CC(=O)CC(O)=O ILJSQTXMGCGYMG-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US52725395A | 1995-09-12 | 1995-09-12 | |
| US08/527,253 | 1995-09-12 | ||
| CA002205057A CA2205057C (fr) | 1995-09-12 | 1996-09-10 | Dispositif et procede d'amplification et de dosage d'adn |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002205057A Division CA2205057C (fr) | 1995-09-12 | 1996-09-10 | Dispositif et procede d'amplification et de dosage d'adn |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2304945A1 true CA2304945A1 (fr) | 1997-03-20 |
Family
ID=25679322
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002304945A Abandoned CA2304945A1 (fr) | 1995-09-12 | 1996-09-10 | Dispositif et procede d'amplification et de dosage d'adn |
Country Status (1)
| Country | Link |
|---|---|
| CA (1) | CA2304945A1 (fr) |
-
1996
- 1996-09-10 CA CA002304945A patent/CA2304945A1/fr not_active Abandoned
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| FZDE | Dead |