CA2307832C - Procede de melange ameliore d'une culture cellulaire infectee par la varicelle dans une bouteille pour agitateur rotatif - Google Patents

Procede de melange ameliore d'une culture cellulaire infectee par la varicelle dans une bouteille pour agitateur rotatif Download PDF

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Publication number
CA2307832C
CA2307832C CA002307832A CA2307832A CA2307832C CA 2307832 C CA2307832 C CA 2307832C CA 002307832 A CA002307832 A CA 002307832A CA 2307832 A CA2307832 A CA 2307832A CA 2307832 C CA2307832 C CA 2307832C
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CA
Canada
Prior art keywords
bottle
rotation
flow
roller bottle
mixing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA002307832A
Other languages
English (en)
Other versions
CA2307832A1 (fr
Inventor
Joye L. Bramble
James A. Searles
Fernando J. Muzzio
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Merck Sharp and Dohme LLC
Original Assignee
Merck and Co Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB9727130.8A external-priority patent/GB9727130D0/en
Application filed by Merck and Co Inc filed Critical Merck and Co Inc
Publication of CA2307832A1 publication Critical patent/CA2307832A1/fr
Application granted granted Critical
Publication of CA2307832C publication Critical patent/CA2307832C/fr
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/10—Rotating vessel
    • C12M27/12—Roller bottles; Roller tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/16—Vibrating; Shaking; Tilting

Landscapes

  • Wood Science & Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention concerne un procédé permettant d'améliorer le mélange d'une culture cellulaire infectée par la varicelle dans une bouteille pour agitateur rotatif, ce procédé consistant à introduire de manière régulée des perturbations selon un écoulement transversal au cours du pivotement de ladite bouteille pour agitateur rotatif. L'efficacité de ce procédé se traduit par un rendement amélioré des mises en culture cellulaire et une meilleure propagation virale dans les bouteilles pour agitateur rotatif, grâce à l'introduction des perturbations susmentionnées au cours du processus.
CA002307832A 1997-10-31 1998-10-27 Procede de melange ameliore d'une culture cellulaire infectee par la varicelle dans une bouteille pour agitateur rotatif Expired - Fee Related CA2307832C (fr)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US6444897P 1997-10-31 1997-10-31
US60/064,448 1997-10-31
US6726497P 1997-12-03 1997-12-03
US60/067,264 1997-12-03
GBGB9727130.8A GB9727130D0 (en) 1997-12-22 1997-12-22 A method of improved mixing of a varicella-infected cell culture in roller bottles
GB9727130.8 1997-12-22
PCT/US1998/022716 WO1999023206A1 (fr) 1997-10-31 1998-10-27 Procede de melange ameliore d'une culture cellulaire infectee par la varicelle dans une bouteille pour agitateur rotatif

Publications (2)

Publication Number Publication Date
CA2307832A1 CA2307832A1 (fr) 1999-05-14
CA2307832C true CA2307832C (fr) 2005-09-06

Family

ID=27269149

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002307832A Expired - Fee Related CA2307832C (fr) 1997-10-31 1998-10-27 Procede de melange ameliore d'une culture cellulaire infectee par la varicelle dans une bouteille pour agitateur rotatif

Country Status (5)

Country Link
EP (1) EP1027426A4 (fr)
JP (1) JP2001521737A (fr)
AU (1) AU742756B2 (fr)
CA (1) CA2307832C (fr)
WO (1) WO1999023206A1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2295465T3 (es) 2002-09-16 2008-04-16 Pan-Biotech Gmbh Procedimiento para el cultivo de celulas, en especial de celulas humanas o de animales.

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3893887A (en) * 1973-07-09 1975-07-08 Univ Duke Method for rapid harvesting of roller culture supernatant fluid
US3982259A (en) * 1974-12-13 1976-09-21 Edward Van Baerle Photographic material processing module
US4000256A (en) * 1975-04-30 1976-12-28 Merck & Co., Inc. Varicella vaccine and process for its preparation
DE2934328C2 (de) * 1979-08-24 1982-04-29 Stephan Dipl.-Chem. Dr.rer.nat. 8000 München Nees Verfahren zur Kultivierung matrixgebundener biologischer Zellsysteme sowie Vorrichtung zur Ausübung des Verfahrens
US4307965A (en) * 1980-05-30 1981-12-29 Innovative Medical Systems Corp. Mixing apparatus
US4317811A (en) * 1980-09-11 1982-03-02 Merck & Co., Inc. Herpes simplex type 1 subunit vaccine
JPS61138522A (ja) * 1984-12-11 1986-06-26 Matsushita Electric Ind Co Ltd 混合機
US4686101A (en) * 1985-08-02 1987-08-11 Merck & Co., Inc. Vaccine against varicella-zoster virus
ATE77830T1 (de) * 1986-03-14 1992-07-15 Forschungszentrum Juelich Gmbh Verfahren zur durchfuehrung gasbildender biotechnologischer prozesse in festbettreaktoren und zum be- und entgasen geeignete vorrichtungen.
US5026650A (en) * 1988-06-30 1991-06-25 The United States Of Amercia As Represented By The Administrator Of The National Aeronautics And Space Administration Horizontally rotated cell culture system with a coaxial tubular oxygenator
JPH0568533A (ja) * 1991-09-12 1993-03-23 Hitachi Ltd 生物細胞の培養方法
DE4229334C2 (de) * 1992-09-02 1995-12-21 Heraeus Instr Gmbh Rollflaschenähnliches Kulturgefäß für Zellkulturen
IL114501A (en) * 1994-07-14 1999-08-17 Akzo Nobel Nv Marek's disease virus vaccine
DE19612967A1 (de) * 1996-04-01 1997-10-02 Behringwerke Ag Verfahren zur Vermehrung von Influenzaviren in Zellkultur, sowie die durch das Verfahren erhältlichen Influenzaviren

Also Published As

Publication number Publication date
AU742756B2 (en) 2002-01-10
EP1027426A1 (fr) 2000-08-16
CA2307832A1 (fr) 1999-05-14
WO1999023206A1 (fr) 1999-05-14
EP1027426A4 (fr) 2006-04-19
AU1280499A (en) 1999-05-24
JP2001521737A (ja) 2001-11-13

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Effective date: 20131029