CA2324422A1 - Variants de serine-proteases ayant des deletions et des substitutions d'acides amines - Google Patents

Variants de serine-proteases ayant des deletions et des substitutions d'acides amines Download PDF

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Publication number
CA2324422A1
CA2324422A1 CA002324422A CA2324422A CA2324422A1 CA 2324422 A1 CA2324422 A1 CA 2324422A1 CA 002324422 A CA002324422 A CA 002324422A CA 2324422 A CA2324422 A CA 2324422A CA 2324422 A1 CA2324422 A1 CA 2324422A1
Authority
CA
Canada
Prior art keywords
ala
gly
asn
variants
leu
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002324422A
Other languages
English (en)
Inventor
Donn Nelton Rubingh
Elizabeth Ellen Sikorski
Paul Elliott Correa
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Procter and Gamble Co
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2324422A1 publication Critical patent/CA2324422A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
    • A61K8/0208—Tissues; Wipes; Patches
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
    • A61K8/66—Enzymes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/86—Products or compounds obtained by genetic engineering
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q11/00—Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/10—Washing or bathing preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q5/00—Preparations for care of the hair

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Veterinary Medicine (AREA)
  • Biomedical Technology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Biotechnology (AREA)
  • Epidemiology (AREA)
  • Birds (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Dermatology (AREA)
  • Detergent Compositions (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Cosmetics (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

Cette invention se rapporte à des variants de sérine-protéases ayant une immunogénicité inférieure à celle des protéases du type sauvage correspondantes. Cette invention se rapporte plus particulièrement à des variants ayant une séquence d'acides aminés modifiée par rapport à une séquence d'acides aminés de type sauvage, cette séquence d'acides aminés modifiée comportant une délétion et, éventuellement, une substitution d'une ou de plusieurs positions spécifiquement identifiées correspondant à la subtilisine BPN'. Cette invention se rapporte en outre à des gènes mutants codant ces variants et à des compositions de produits de nettoyage et de produits de soins personnels comprenant ces variants.
CA002324422A 1998-03-26 1999-03-25 Variants de serine-proteases ayant des deletions et des substitutions d'acides amines Abandoned CA2324422A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US7947798P 1998-03-26 1998-03-26
US60/079,477 1998-03-26
PCT/IB1999/000520 WO1999049057A1 (fr) 1998-03-26 1999-03-25 Variants de serine-proteases ayant des deletions et des substitutions d'acides amines

Publications (1)

Publication Number Publication Date
CA2324422A1 true CA2324422A1 (fr) 1999-09-30

Family

ID=22150818

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002324422A Abandoned CA2324422A1 (fr) 1998-03-26 1999-03-25 Variants de serine-proteases ayant des deletions et des substitutions d'acides amines

Country Status (9)

Country Link
EP (1) EP1082443A1 (fr)
JP (1) JP2002518993A (fr)
KR (1) KR20010052223A (fr)
CN (1) CN1303437A (fr)
AR (1) AR023021A1 (fr)
AU (1) AU750336B2 (fr)
BR (1) BR9909147A (fr)
CA (1) CA2324422A1 (fr)
WO (1) WO1999049057A1 (fr)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1373802A (zh) * 1999-07-22 2002-10-09 宝洁公司 在特定表位区域具有氨基酸缺失和取代的枯草杆菌蛋白酶变体
US6803222B2 (en) * 2000-11-22 2004-10-12 Kao Corporation Alkaline proteases
DE10162727A1 (de) 2001-12-20 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus gibsonii (DSM 14391) und Wasch-und Reinigungsmittel enthaltend diese neue Alkalische Protease
DE10162728A1 (de) 2001-12-20 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus gibsonii (DSM 14393) und Wasch-und Reinigungsmittel enthaltend diese neue Alkalische Protease
DE10163884A1 (de) 2001-12-22 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus sp. (DSM 14392) und Wasch- und Reinigungsmittel enthaltend diese neue Alkalische Protease
DE10260903A1 (de) 2002-12-20 2004-07-08 Henkel Kgaa Neue Perhydrolasen
DE10360805A1 (de) 2003-12-23 2005-07-28 Henkel Kgaa Neue Alkalische Protease und Wasch- und Reinigungsmittel, enthaltend diese neue Alkalische Protease
DE102007003143A1 (de) 2007-01-16 2008-07-17 Henkel Kgaa Neue Alkalische Protease aus Bacillus gibsonii und Wasch- und Reinigungsmittel enthaltend diese neue Alkalische Protease
DE102007032111B4 (de) 2007-07-09 2017-07-20 Henkel Ag & Co. Kgaa Neue Proteasen und Wasch- und Reinigungsmittel enthaltend diese Proteasen
DE102007036756A1 (de) 2007-08-03 2009-02-05 Henkel Ag & Co. Kgaa Neue Proteasen und Wasch- und Reinigungsmittel, enthaltend diese neuen Proteasen
DE102007051092A1 (de) 2007-10-24 2009-04-30 Henkel Ag & Co. Kgaa Subtilisin aus Becillus pumilus und Wasch- und Reinigungsmittel enthaltend dieses neue Subtilisin
AR076312A1 (es) * 2009-04-24 2011-06-01 Danisco Us Inc Proteasas con regiones pro modificadas
CN102648277B (zh) * 2009-09-25 2015-05-20 诺维信公司 蛋白酶变体的用途
WO2013076269A1 (fr) * 2011-11-25 2013-05-30 Novozymes A/S Variants de subtilase et polynucléotides codants pour ceux-ci
CN102839165B (zh) * 2012-09-26 2014-12-10 金普诺安生物科技（苏州）有限公司 基因突变型重组蛋白酶k及其工业化生产方法
US12497644B2 (en) 2021-02-12 2025-12-16 Fresenius Kabi Ipsum S.R.L. Subtilisin variants and their use

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3856593D1 (de) * 1987-04-06 2007-10-04 Novozymes As Regelung von elektrostatischen interaktionen an metallionen-Bindungsstellen zur Stabilisierung von Proteinen
BR9006827A (pt) * 1989-06-26 1991-08-06 Unilever Nv Composicoes detergentes enzimaticas
FI932561L (fi) * 1990-12-05 1993-06-04 Novo Nordisk As Proteiner med foeraendrade epitoper och foerfaranden foer deras framstaellning
US5567601A (en) * 1993-06-01 1996-10-22 University Of Maryland Subtilisin mutants lacking a primary calcium binding site
US6569663B1 (en) * 1998-03-26 2003-05-27 The Procter & Gamble Company Serine protease variants having amino acid substitutions

Also Published As

Publication number Publication date
EP1082443A1 (fr) 2001-03-14
BR9909147A (pt) 2000-12-05
KR20010052223A (ko) 2001-06-25
WO1999049057A1 (fr) 1999-09-30
AU750336B2 (en) 2002-07-18
AR023021A1 (es) 2002-09-04
JP2002518993A (ja) 2002-07-02
CN1303437A (zh) 2001-07-11
AU2742699A (en) 1999-10-18

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