CA2344591A1 - Methodes permettant d'ameliorer la fonction des recepteurs heterologues couples a la proteine g - Google Patents
Methodes permettant d'ameliorer la fonction des recepteurs heterologues couples a la proteine g Download PDFInfo
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- CA2344591A1 CA2344591A1 CA002344591A CA2344591A CA2344591A1 CA 2344591 A1 CA2344591 A1 CA 2344591A1 CA 002344591 A CA002344591 A CA 002344591A CA 2344591 A CA2344591 A CA 2344591A CA 2344591 A1 CA2344591 A1 CA 2344591A1
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- MMWCIQZXVOZEGG-HOZKJCLWSA-N [(1S,2R,3S,4S,5R,6S)-2,3,5-trihydroxy-4,6-diphosphonooxycyclohexyl] dihydrogen phosphate Chemical compound O[C@H]1[C@@H](O)[C@H](OP(O)(O)=O)[C@@H](OP(O)(O)=O)[C@H](O)[C@H]1OP(O)(O)=O MMWCIQZXVOZEGG-HOZKJCLWSA-N 0.000 description 1
- 230000000895 acaricidal effect Effects 0.000 description 1
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- 238000009825 accumulation Methods 0.000 description 1
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- 229940126157 adrenergic receptor agonist Drugs 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- RKUNBYITZUJHSG-SPUOUPEWSA-N atropine Chemical compound O([C@H]1C[C@H]2CC[C@@H](C1)N2C)C(=O)C(CO)C1=CC=CC=C1 RKUNBYITZUJHSG-SPUOUPEWSA-N 0.000 description 1
- 229960000396 atropine Drugs 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000008238 biochemical pathway Effects 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
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- 229960002896 clonidine Drugs 0.000 description 1
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- 239000003814 drug Substances 0.000 description 1
- 238000007876 drug discovery Methods 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 210000003527 eukaryotic cell Anatomy 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 102000034356 gene-regulatory proteins Human genes 0.000 description 1
- 108091006104 gene-regulatory proteins Proteins 0.000 description 1
- 238000012252 genetic analysis Methods 0.000 description 1
- MASNOZXLGMXCHN-ZLPAWPGGSA-N glucagon Chemical compound C([C@@H](C(=O)N[C@H](C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(O)=O)C(C)C)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](C)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CO)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CO)NC(=O)[C@@H](NC(=O)[C@H](CC=1C=CC=CC=1)NC(=O)[C@@H](NC(=O)CNC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC=1NC=NC=1)[C@@H](C)O)[C@@H](C)O)C1=CC=CC=C1 MASNOZXLGMXCHN-ZLPAWPGGSA-N 0.000 description 1
- 229960004666 glucagon Drugs 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 101150087371 gpd1 gene Proteins 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 102000045019 human CHRM2 Human genes 0.000 description 1
- 230000006303 immediate early viral mRNA transcription Effects 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 230000000749 insecticidal effect Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
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- 230000009456 molecular mechanism Effects 0.000 description 1
- 238000002703 mutagenesis Methods 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- PCJGZPGTCUMMOT-ISULXFBGSA-N neurotensin Chemical compound C([C@@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC(C)C)C(O)=O)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CCCCN)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CC(C)C)NC(=O)[C@H]1NC(=O)CC1)C1=CC=C(O)C=C1 PCJGZPGTCUMMOT-ISULXFBGSA-N 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 239000004031 partial agonist Substances 0.000 description 1
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- 238000006366 phosphorylation reaction Methods 0.000 description 1
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- 108060006633 protein kinase Proteins 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
- C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
- C07K14/395—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts from Saccharomyces
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4722—G-proteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/70571—Receptors; Cell surface antigens; Cell surface determinants for neuromediators, e.g. serotonin receptor, dopamine receptor
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/72—Receptors; Cell surface antigens; Cell surface determinants for hormones
- C07K14/723—G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH receptor
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Toxicology (AREA)
- Cell Biology (AREA)
- Immunology (AREA)
- Mycology (AREA)
- Endocrinology (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Neurology (AREA)
- Microbiology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
L'invention concerne des récepteurs mutants couplés à la protéine G, lesquels présentent un meilleur couplage à la protéine G et une meilleure réponse de réception. L'invention concerne également des cellules de levure exprimant ces récepteurs, des vecteurs convenant à la fabrication de ces cellules et des méthodes de fabrication et d'utilisation associées.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US9870498P | 1998-09-01 | 1998-09-01 | |
| US60/098,704 | 1998-09-01 | ||
| PCT/US1999/020013 WO2000012705A2 (fr) | 1998-09-01 | 1999-09-01 | Methodes permettant d'ameliorer la fonction des recepteurs heterologues couples a la proteine g |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2344591A1 true CA2344591A1 (fr) | 2000-03-09 |
Family
ID=22270543
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002344591A Abandoned CA2344591A1 (fr) | 1998-09-01 | 1999-09-01 | Methodes permettant d'ameliorer la fonction des recepteurs heterologues couples a la proteine g |
| CA002343046A Abandoned CA2343046A1 (fr) | 1998-09-01 | 1999-09-01 | Expression fonctionnelle amelioree des recepteurs couples a la proteine g |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002343046A Abandoned CA2343046A1 (fr) | 1998-09-01 | 1999-09-01 | Expression fonctionnelle amelioree des recepteurs couples a la proteine g |
Country Status (5)
| Country | Link |
|---|---|
| EP (2) | EP1123391A2 (fr) |
| JP (2) | JP2002523091A (fr) |
| AU (2) | AU5700999A (fr) |
| CA (2) | CA2344591A1 (fr) |
| WO (2) | WO2000012705A2 (fr) |
Families Citing this family (30)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6528271B1 (en) | 1997-06-05 | 2003-03-04 | Duke University | Inhibition of βarrestin mediated effects prolongs and potentiates opioid receptor-mediated analgesia |
| US5891646A (en) | 1997-06-05 | 1999-04-06 | Duke University | Methods of assaying receptor activity and constructs useful in such methods |
| US7541151B2 (en) | 1997-06-05 | 2009-06-02 | Duke University | Single-cell biosensor for the measurement of GPCR ligands in a test sample |
| NZ521696A (en) * | 2000-04-24 | 2004-06-25 | Wyeth Corp | Transgenic animal models for diseases that are regulated by G-protein signalling |
| CA2426859A1 (fr) * | 2000-10-26 | 2002-05-02 | The University Of British Columbia | Essai fonctionnel a base de cellules d'insectes concernant les recepteurs couples aux proteines g |
| EP1331228B1 (fr) * | 2000-11-01 | 2007-03-21 | Astellas Pharma Inc. | Procede de criblage d'anti-plaquettes |
| US7163800B2 (en) | 2000-11-03 | 2007-01-16 | Molecular Devices Corporation | Methods of screening compositions for G protein-coupled receptor desensitization inhibitory activity |
| US7018812B2 (en) | 2000-11-03 | 2006-03-28 | Duke University | Modified G-protein coupled receptors |
| DE10056899A1 (de) | 2000-11-16 | 2002-05-29 | Aventis Pharma Gmbh | Promotor zur funktionellen Charakterisierung von G-Protein gekoppelten Rezeptoren in der Hefe Saccharomyces cerevisiae |
| AU2002228844A1 (en) * | 2000-12-06 | 2002-06-18 | Deltagen, Inc. | Transgenic mice containing targeted gene disruptions |
| GB0030038D0 (en) * | 2000-12-08 | 2001-01-24 | Univ Warwick | Yeast-based assay |
| US7279324B2 (en) | 2001-01-23 | 2007-10-09 | Duke University | Nucleic acid encoding G-protein coupled receptor with modified DRY motif |
| EP1336655A1 (fr) * | 2002-01-12 | 2003-08-20 | Aventis Pharma Deutschland GmbH | Méthode pour l'identification des mutants ayant une actività constitutive |
| DE10233516A1 (de) * | 2002-07-23 | 2004-02-12 | Aventis Pharma Deutschland Gmbh | Verfahren zur Identifizierung von Substanzen |
| WO2006104254A1 (fr) * | 2005-03-29 | 2006-10-05 | Bio-Energy Corporation | Procede pour le criblage d'une substance de liaison des recepteurs |
| US8354241B2 (en) | 2005-04-27 | 2013-01-15 | Arena Pharmaceuticals, Inc. | Methods for identifying a GLP-1 secretagogue |
| AU2006264649A1 (en) | 2005-06-30 | 2007-01-11 | Prosidion Limited | GPCR agonists |
| NZ571110A (en) | 2006-04-11 | 2011-03-31 | Arena Pharm Inc | Methods of using GPR119 receptor to identify Glucose-dependent Insulinotropic Peptide (GIP) secretagogue compounds useful for increasing bone mass in an individual |
| CL2008000018A1 (es) | 2007-01-04 | 2008-08-01 | Prosidion Ltd | Compuestos derivados de heterociclos de nitrogeno y oxigeno, agonistas de gpcr; composicion farmaceutica que comprende a dicho compuesto; y uso del compuesto para el tratamiento de la obesidad, diabetes, sindrome metabolico, hiperlipidemia, toleranci |
| EP2114933B1 (fr) | 2007-01-04 | 2011-09-07 | Prosidion Ltd | Agonistes du gpcr de pipéridine |
| PE20081849A1 (es) | 2007-01-04 | 2009-01-26 | Prosidion Ltd | Derivados de piperidin-4-il-propoxi-benzamida como agonistas de gpcr |
| GB2456236B8 (en) * | 2007-03-22 | 2009-12-09 | Heptares Therapeutics Ltd | Stable muscarinic receptor mutants |
| JP2010539152A (ja) | 2007-09-10 | 2010-12-16 | プロシディオン・リミテッド | 代謝障害の治療のための化合物 |
| GB0720390D0 (en) | 2007-10-18 | 2007-11-28 | Prosidion Ltd | G-Protein coupled receptor agonists |
| GB0720389D0 (en) | 2007-10-18 | 2008-11-12 | Prosidion Ltd | G-Protein Coupled Receptor Agonists |
| EP2146210A1 (fr) | 2008-04-07 | 2010-01-20 | Arena Pharmaceuticals, Inc. | Procédés d'utilisation du récepteur couplé aux protéines A G pour identifier les secrétagogues de peptide YY (PYY) et composés utiles dans le traitement d'états modulés par PYY |
| GB0904285D0 (en) | 2009-03-12 | 2009-04-22 | Prosidion Ltd | Compounds for the treatment of metabolic disorders |
| GB0904284D0 (en) | 2009-03-12 | 2009-04-22 | Prosidion Ltd | Compounds for the treatment of metabolic disorders |
| GB0904287D0 (en) | 2009-03-12 | 2009-04-22 | Prosidion Ltd | Compounds for the treatment of metabolic disorders |
| JP7422498B2 (ja) * | 2019-07-25 | 2024-01-26 | 芳武 村山 | ペプチド、細胞遊走抑制剤、細胞浸潤抑制剤、がん細胞転移抑制剤、及び医薬 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5691188A (en) * | 1994-02-14 | 1997-11-25 | American Cyanamid Company | Transformed yeast cells expressing heterologous G-protein coupled receptor |
| CA2193809A1 (fr) * | 1994-06-29 | 1996-01-11 | Catherine D. Strader | Recepteurs modifies lies a des proteines g |
| US6255089B1 (en) * | 1997-02-27 | 2001-07-03 | Albany Medical College | Constitutively activated serotonin receptors |
-
1999
- 1999-09-01 CA CA002344591A patent/CA2344591A1/fr not_active Abandoned
- 1999-09-01 AU AU57009/99A patent/AU5700999A/en not_active Abandoned
- 1999-09-01 EP EP99944035A patent/EP1123391A2/fr not_active Withdrawn
- 1999-09-01 EP EP99944033A patent/EP1112359A2/fr not_active Withdrawn
- 1999-09-01 JP JP2000567692A patent/JP2002523091A/ja not_active Withdrawn
- 1999-09-01 CA CA002343046A patent/CA2343046A1/fr not_active Abandoned
- 1999-09-01 AU AU57011/99A patent/AU756244B2/en not_active Ceased
- 1999-09-01 JP JP2000567691A patent/JP2002523090A/ja not_active Withdrawn
- 1999-09-01 WO PCT/US1999/020013 patent/WO2000012705A2/fr not_active Ceased
- 1999-09-01 WO PCT/US1999/020011 patent/WO2000012704A2/fr not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| AU5701199A (en) | 2000-03-21 |
| WO2000012704A9 (fr) | 2000-06-08 |
| AU756244B2 (en) | 2003-01-09 |
| WO2000012704A3 (fr) | 2000-11-30 |
| JP2002523090A (ja) | 2002-07-30 |
| WO2000012704A2 (fr) | 2000-03-09 |
| WO2000012705A9 (fr) | 2000-07-13 |
| WO2000012705A3 (fr) | 2000-10-05 |
| JP2002523091A (ja) | 2002-07-30 |
| EP1123391A2 (fr) | 2001-08-16 |
| CA2343046A1 (fr) | 2000-03-09 |
| EP1112359A2 (fr) | 2001-07-04 |
| WO2000012705A2 (fr) | 2000-03-09 |
| AU5700999A (en) | 2000-03-21 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FZDE | Discontinued |