CA2347004A1 - Procede de concentration de micro-organismes au moyen d'une separation par affinite - Google Patents

Procede de concentration de micro-organismes au moyen d'une separation par affinite Download PDF

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Publication number
CA2347004A1
CA2347004A1 CA002347004A CA2347004A CA2347004A1 CA 2347004 A1 CA2347004 A1 CA 2347004A1 CA 002347004 A CA002347004 A CA 002347004A CA 2347004 A CA2347004 A CA 2347004A CA 2347004 A1 CA2347004 A1 CA 2347004A1
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CA
Canada
Prior art keywords
matrix
sample
microorganisms
cbd
cfu
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002347004A
Other languages
English (en)
Inventor
Daniel L. Siegel
Anthony Saunders
Oded Shoseyov
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
CBD Technologies Ltd
Yissum Research Development Co of Hebrew University of Jerusalem
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2347004A1 publication Critical patent/CA2347004A1/fr
Abandoned legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/02—Separating microorganisms from their culture media
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Sustainable Development (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne un procédé permettant de concentrer un(des) micro-organisme(s) particulier(s) ou d'intérêt dans un échantillon. Pour ce faire, on met l'échantillon en contact avec une matrice à laquelle est lié un récepteur d'affinité spécifique au(x) micro-organisme(s) particulier(s), le récepteur d'affinité et la matrice étant sélectionnés de manière à permettre la capture du(des) micro-organisme(s) vers la matrice au moyen du récepteur d'affinité, lorsque le(s) micro-organisme(s) est(sont) présent(s) en quantité négligeable dans l'échantillon. Aussi, l'étape de pré-enrichissement prolongée est-elle nécessaire et dans certains cas, il faut la combiner avec une étape de pré-enrichissement du(des) micro-organisme(s) dans l'échantillon.
CA002347004A 1998-10-19 1999-08-04 Procede de concentration de micro-organismes au moyen d'une separation par affinite Abandoned CA2347004A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US17504098A 1998-10-19 1998-10-19
US09/175,040 1998-10-19
US30145199A 1999-04-29 1999-04-29
US09/301,451 1999-04-29
PCT/US1999/017589 WO2000023792A1 (fr) 1998-10-19 1999-08-04 Procede de concentration de micro-organismes au moyen d'une separation par affinite

Publications (1)

Publication Number Publication Date
CA2347004A1 true CA2347004A1 (fr) 2000-04-27

Family

ID=26870805

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002347004A Abandoned CA2347004A1 (fr) 1998-10-19 1999-08-04 Procede de concentration de micro-organismes au moyen d'une separation par affinite

Country Status (4)

Country Link
EP (1) EP1123501A4 (fr)
AU (1) AU5670299A (fr)
CA (1) CA2347004A1 (fr)
WO (1) WO2000023792A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11187631B2 (en) * 2016-09-20 2021-11-30 Kabcyt Device for capturing biological particles

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CN1320631A (zh) * 2000-04-27 2001-11-07 上海博德基因开发有限公司 一种新的多肽——人含纤维素结合结构域的蛋白10和编码这种多肽的多核苷酸
GB0010910D0 (en) * 2000-05-05 2000-06-28 Jones Osborn Analytical method and apparatus
ATE366301T1 (de) * 2001-02-05 2007-07-15 Millipore Corp Detektion von mikroorganismen
EP1253203A1 (fr) 2001-04-25 2002-10-30 Becton Dickinson and Company Germination, développement, immobilisation et détéction rapide des microorganismes
GB0112428D0 (en) * 2001-05-22 2001-07-11 Secr Defence Brit Identifying micro-organisms
US20030022358A1 (en) * 2001-05-29 2003-01-30 Hall Jane H. Growth of filterable organisms that require non-diffusable media components
DE10129815A1 (de) * 2001-06-24 2003-01-09 Profos Ag Verfahren zur Aufreinigung von Bakterienzellen und Zellbestandteilen
US7569025B2 (en) 2002-04-02 2009-08-04 Scantibodies Laboratory, Inc. Methods and devices for treating severe peripheral bacterial infections
GB2387130A (en) 2002-04-04 2003-10-08 Fluid Technologies Plc Hollow fibre filter membrane unit with microorganism detector, and associated usage
EP1909833A2 (fr) * 2005-06-20 2008-04-16 Scantibodies Laboratory, Inc. Procédés et dispositifs destinés à éliminer des pathogènes et des substances nuisibles des liquides organiques
DK1960016T3 (en) 2005-12-13 2017-01-09 Exthera Medical Corp Method for extracorporeal removal by a pathogenic MI-KROBE, an inflammatory cell or inflammatory protein from the blood.
EP2092071B1 (fr) * 2006-12-14 2014-11-12 Technion Research & Development Foundation Ltd. Compositions et procedes de concentration et d'appauvrissement de micro-organismes
CN101215598B (zh) * 2008-01-11 2010-11-17 中华人民共和国吉林出入境检验检疫局 免疫富集丝检测细菌的方法及免疫富集刷
BRPI0918693A2 (pt) 2008-12-31 2016-07-26 3M Innovative Properties Co dispositivos para amostragem e métodos para concentração de microorganismos
EP2379738B1 (fr) * 2008-12-31 2017-01-25 3M Innovative Properties Company Procede de detection de coliformes
EP2509604B1 (fr) 2009-12-01 2021-03-17 ExThera Medical Corporation Dispositif pour éliminer de cytokines du sang par des polysaccharides immobilisés en surface
WO2012112724A1 (fr) 2011-02-15 2012-08-23 Exthera Medical, Llc Dispositifs et procédé d'élimination de toxines, de cytokines inflammatoires et de pathogènes à diffusion hématogène
CN104284984B (zh) * 2012-02-29 2017-10-13 哈佛大学校长及研究员协会 抗生素药敏性的快速测试
EP2861273B1 (fr) 2012-06-13 2017-08-23 ExThera Medical Corporation Utilisation de l'héparine et des hydrates de carbone pour traiter le cancer
MX376935B (es) 2013-06-24 2025-03-07 Exthera Medical Corp Sistema de filtración de sangre que contiene substrato recubierto con manosa.
CA2928866C (fr) * 2013-11-08 2021-11-09 Exthera Medical Corporation Procedes pour diagnostiquer des maladies infectieuses a l'aide de milieux d'adsorption
AU2015250107A1 (en) 2014-04-24 2016-11-17 Exthera Medical Corporation Method for removing bacteria from blood using high flow rate
CA2959975A1 (fr) 2014-09-22 2016-03-31 Exthera Medical Corporation Dispositif d'hemoperfusion pouvant etre porte sur soi
US11911551B2 (en) 2016-03-02 2024-02-27 Exthera Medical Corporation Method for treating drug intoxication
US10786615B2 (en) 2016-03-02 2020-09-29 Exthera Medical Corporation Method for treating drug intoxication
WO2020231830A1 (fr) 2019-05-16 2020-11-19 Exthera Medical Corporation Procédé de modulation de la structure du glycocalyx endothélial
CN119318642B (zh) * 2024-12-19 2025-03-07 深圳万可森生物科技有限公司 纳米淀粉颗粒载药系统及在防治水产动物病害中的应用

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US4563418A (en) * 1982-04-09 1986-01-07 Bio-Controls Systems, Inc. Process for detection of selected motile organisms
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11187631B2 (en) * 2016-09-20 2021-11-30 Kabcyt Device for capturing biological particles

Also Published As

Publication number Publication date
AU5670299A (en) 2000-05-08
EP1123501A1 (fr) 2001-08-16
WO2000023792A1 (fr) 2000-04-27
EP1123501A4 (fr) 2005-04-13

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Legal Events

Date Code Title Description
FZDE Discontinued