CA2348054C - Procedes et moyens pour l'isolement et la purification d'acides nucleiques sur des surfaces - Google Patents

Procedes et moyens pour l'isolement et la purification d'acides nucleiques sur des surfaces Download PDF

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Publication number
CA2348054C
CA2348054C CA2348054A CA2348054A CA2348054C CA 2348054 C CA2348054 C CA 2348054C CA 2348054 A CA2348054 A CA 2348054A CA 2348054 A CA2348054 A CA 2348054A CA 2348054 C CA2348054 C CA 2348054C
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CA
Canada
Prior art keywords
nucleic acids
membrane
rna
buffer
isolation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA2348054A
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English (en)
Other versions
CA2348054A1 (fr
Inventor
Simone Gauch
Helge Bastian
Susanne Ullmann
Uwe Oelmuller
Martin Weber
Guido Fuhrmann
Joachim Schorr
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Qiagen GmbH
Original Assignee
Qiagen GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/EP1998/006756 external-priority patent/WO1999022021A1/fr
Application filed by Qiagen GmbH filed Critical Qiagen GmbH
Priority to CA2771000A priority Critical patent/CA2771000A1/fr
Publication of CA2348054A1 publication Critical patent/CA2348054A1/fr
Application granted granted Critical
Publication of CA2348054C publication Critical patent/CA2348054C/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5023—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures with a sample being transported to, and subsequently stored in an absorbent for analysis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Clinical Laboratory Science (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Detergent Compositions (AREA)
  • Cleaning Or Drying Semiconductors (AREA)
  • Cleaning By Liquid Or Steam (AREA)

Abstract

La présente invention concerne de nouveaux procédés et appareils pour l'isolement et la purification d'acides nucléiques sur des surfaces. Elle porte sur des procédés utilisant des surfaces, par exemple des membranes poreuses, sur lesquelles les acides nucléiques peuvent être immobilisés de manière simple à partir de l'échantillon les contenant et peuvent être à nouveau séparés au moyen d'étapes de procédé également simples, cela permettant la conduite du processus selon l'invention de manière simple, et notamment de réaliser les procédés de manière entièrement automatique. Un autre aspect de la présente invention porte sur la fixation d'acides nucléiques sur une phase immobile, notamment sur une membrane, de telle manière qu'ils puissent être à nouveau séparés de cette phase en toute simplicité lors d'une étape de réaction subséquente et éventuellement être utilisés dans d'autres applications, comme par exemple la digestion par restriction, la RT, la PCR ou la RT-PCR ou bien dans chaque autre réaction analytique ou enzymatique appropriée précitée. Finalement l'invention porte sur des récipients d'isolement spéciaux permettant de réaliser les procédés décrits.
CA2348054A 1998-10-23 1999-04-20 Procedes et moyens pour l'isolement et la purification d'acides nucleiques sur des surfaces Expired - Lifetime CA2348054C (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA2771000A CA2771000A1 (fr) 1998-10-23 1999-04-20 Procedes et moyens pour l'isolement et la purification d'acides nucleiques sur des surfaces

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EPPCT/EP98/06756 1998-10-23
PCT/EP1998/006756 WO1999022021A1 (fr) 1997-10-23 1998-10-23 Procede pour isoler et purifier des acides nucleiques sur des surfaces
PCT/EP1999/002664 WO2000024927A1 (fr) 1997-10-23 1999-04-20 Procedes et moyens pour l'isolement et la purification d'acides nucleiques sur des surfaces

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CA2771000A Division CA2771000A1 (fr) 1998-10-23 1999-04-20 Procedes et moyens pour l'isolement et la purification d'acides nucleiques sur des surfaces

Publications (2)

Publication Number Publication Date
CA2348054A1 CA2348054A1 (fr) 2000-05-04
CA2348054C true CA2348054C (fr) 2012-06-19

Family

ID=8167111

Family Applications (2)

Application Number Title Priority Date Filing Date
CA2348054A Expired - Lifetime CA2348054C (fr) 1998-10-23 1999-04-20 Procedes et moyens pour l'isolement et la purification d'acides nucleiques sur des surfaces
CA2771000A Abandoned CA2771000A1 (fr) 1998-10-23 1999-04-20 Procedes et moyens pour l'isolement et la purification d'acides nucleiques sur des surfaces

Family Applications After (1)

Application Number Title Priority Date Filing Date
CA2771000A Abandoned CA2771000A1 (fr) 1998-10-23 1999-04-20 Procedes et moyens pour l'isolement et la purification d'acides nucleiques sur des surfaces

Country Status (6)

Country Link
JP (2) JP2002528093A (fr)
AT (1) ATE474063T1 (fr)
AU (1) AU765375C (fr)
CA (2) CA2348054C (fr)
DE (1) DE59915184D1 (fr)
DK (1) DK1121460T3 (fr)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2527216A1 (fr) * 2003-05-28 2004-12-09 Qiagen Gmbh Procede pour obtenir un adn plasmidique au moyen d'un systeme aqueux 2-phase
JP4831725B2 (ja) * 2004-02-13 2011-12-07 栄研化学株式会社 簡易的核酸抽出法
JP2006217839A (ja) * 2005-02-09 2006-08-24 Fuji Photo Film Co Ltd 核酸の分離精製方法
JP5627162B2 (ja) * 2005-10-05 2014-11-19 倉敷紡績株式会社 核酸の分離精製方法および当該方法を利用した核酸分離キット
US20080113357A1 (en) * 2006-06-29 2008-05-15 Millipore Corporation Filter device for the isolation of a nucleic acid
WO2013002261A1 (fr) 2011-06-27 2013-01-03 オリンパス株式会社 Procédé de détection de particules cibles
ES2815684T3 (es) * 2015-06-10 2021-03-30 Biocartis N V Detección mejorada de ADN metilado
WO2024079611A1 (fr) * 2022-10-10 2024-04-18 Waters Technologies Corporation Procédé de filtration sur gel entraîné sous vide

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4806546A (en) * 1985-09-30 1989-02-21 Miles Inc. Immobilization of nucleic acids on derivatized nylon supports
JPH02190200A (ja) * 1989-01-19 1990-07-26 Sanyo Chem Ind Ltd 核酸断片の検出法及び装置
JPH0666B2 (ja) * 1989-05-02 1994-01-05 倉敷紡績株式会社 Ｄｎａの単離精製法
JPH0667B2 (ja) * 1989-06-08 1994-01-05 倉敷紡績株式会社 核酸単離用水性組成物
JP2898306B2 (ja) * 1989-07-20 1999-05-31 扶桑薬品工業株式会社 核酸増幅・検出法およびキット
CA2025476A1 (fr) * 1989-09-27 1991-03-28 Shan F. Ching Membranes poreuses, hydrophiles et stratifiees et methodes de preparation
US5136032A (en) * 1989-12-07 1992-08-04 Daicel Chemical Industries, Ltd. Method for separating phosphopolyol compounds using a separating agent
IT1240870B (it) * 1990-02-14 1993-12-17 Talent Procedimento per l'estrazione e la purificazione di dna genomico umano
JPH04187077A (ja) * 1990-11-22 1992-07-03 Shimadzu Corp 核酸の抽出精製装置
JPH04325099A (ja) * 1991-04-25 1992-11-13 Mitsui Petrochem Ind Ltd 核酸の膜への固定法
CA2067711C (fr) * 1991-05-03 2000-08-08 Daniel Lee Woodard Purification par extraction en phase solide de l'adn
JPH0751099A (ja) * 1993-08-11 1995-02-28 Toyobo Co Ltd 核酸配列検査方法及び検査装置
JPH08159932A (ja) * 1994-12-06 1996-06-21 Meito Sangyo Kk 検体採取用具、それを用いる測定方法ならびに測定キツト
JPH08285849A (ja) * 1995-04-14 1996-11-01 Mochida Pharmaceut Co Ltd 簡易測定装置およびこれを用いる測定方法
WO1997003348A1 (fr) * 1995-07-13 1997-01-30 Immunological Associates Of Denver Appareil autonome integrant l'extraction, l'amplification et la detection de l'acide nucleique
JP3641301B2 (ja) * 1995-08-09 2005-04-20 株式会社セルシード 刺激応答型分離材料および分離精製方法
KR19990064358A (ko) * 1995-10-18 1999-07-26 다윈 몰레큘러 코포레이션 하이브리드화용 고체 지지체의 제조 방법 및 비-특이적 배경의 감소 방법
JPH10257887A (ja) * 1996-09-30 1998-09-29 Dainippon Printing Co Ltd 遺伝子解析装置および方法
GB2337261B (en) * 1998-05-15 2002-09-18 Fsm Technologies Ltd Separation of nucleic acids

Also Published As

Publication number Publication date
DK1121460T3 (da) 2010-09-20
CA2771000A1 (fr) 2000-05-04
AU4031599A (en) 2000-05-15
DE59915184D1 (de) 2010-08-26
AU765375C (en) 2004-04-08
CA2348054A1 (fr) 2000-05-04
AU765375B2 (en) 2003-09-18
ATE474063T1 (de) 2010-07-15
JP2002528093A (ja) 2002-09-03
JP2008220377A (ja) 2008-09-25

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