CA2355579C - Enzymes subtilases des sous-groupes i-s1 et i-s2 ayant un residu d'acide amine additionnel dans une region boucle de site actif - Google Patents

Enzymes subtilases des sous-groupes i-s1 et i-s2 ayant un residu d'acide amine additionnel dans une region boucle de site actif Download PDF

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Publication number
CA2355579C
CA2355579C CA2355579A CA2355579A CA2355579C CA 2355579 C CA2355579 C CA 2355579C CA 2355579 A CA2355579 A CA 2355579A CA 2355579 A CA2355579 A CA 2355579A CA 2355579 C CA2355579 C CA 2355579C
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CA
Canada
Prior art keywords
amino acid
enzyme
subtilase
isolated
subtilase enzyme
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
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CA2355579A
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English (en)
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CA2355579A1 (fr
Inventor
Kim Andersen Vilbour
Frank Mikkelsen
Peter Hansen Kamp
Carsten Andersen
Mads Norregaard-Madsen
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Novozymes AS
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Novozymes AS
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Publication date
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Publication of CA2355579A1 publication Critical patent/CA2355579A1/fr
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Publication of CA2355579C publication Critical patent/CA2355579C/fr
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Cette invention se rapporte à des enzymes subtilases des sous-groupes I-S1 et I-S2 comportant un résidu d'acide aminé additionnel dans la position 100 de la région boucle de site actif (b) entre la position 95 et 103. Des variants de ces substilases possèdent des performances de lavage améliorées dans un détergent par rapport à leur enzyme parente.
CA2355579A 1998-12-18 1999-12-20 Enzymes subtilases des sous-groupes i-s1 et i-s2 ayant un residu d'acide amine additionnel dans une region boucle de site actif Expired - Fee Related CA2355579C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DKPA199801673 1998-12-18
DKPA199801673 1998-12-18
PCT/DK1999/000715 WO2000037624A1 (fr) 1998-12-18 1999-12-20 Enzymes subtilases des sous-groupes i-s1 et i-s2 ayant un residu d'acide amine additionnel dans une region boucle de site actif

Publications (2)

Publication Number Publication Date
CA2355579A1 CA2355579A1 (fr) 2000-06-29
CA2355579C true CA2355579C (fr) 2011-11-22

Family

ID=8107093

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2355579A Expired - Fee Related CA2355579C (fr) 1998-12-18 1999-12-20 Enzymes subtilases des sous-groupes i-s1 et i-s2 ayant un residu d'acide amine additionnel dans une region boucle de site actif

Country Status (7)

Country Link
JP (1) JP4647788B2 (fr)
KR (1) KR100660799B1 (fr)
CN (1) CN1302106C (fr)
AU (1) AU773327B2 (fr)
BR (1) BRPI9916350B1 (fr)
CA (1) CA2355579C (fr)
WO (1) WO2000037624A1 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20250311734A1 (en) 2022-05-14 2025-10-09 Novozymes A/S Compositions and Methods for Preventing, Treating, Suppressing and/or Eliminatting Phytopathogenic Infestations and Infections
CN118685387B (zh) * 2024-08-28 2024-10-29 中国农业科学院北京畜牧兽医研究所 一种改进丝氨酸蛋白酶ScAprE的热稳定性的方法及突变体和应用

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK316989D0 (da) * 1989-06-26 1989-06-26 Novo Nordisk As Enzymer
ES2227508T3 (es) * 1989-06-26 2005-04-01 Unilever N.V. Composiciones detergentes enzimaticas.
US6599730B1 (en) * 1994-05-02 2003-07-29 Procter & Gamble Company Subtilisin 309 variants having decreased adsorption and increased hydrolysis
US6455295B1 (en) * 1995-03-08 2002-09-24 The Procter & Gamble Company Subtilisin Carlsberg variants having decreased adsorption and increased hydrolysis
SK284609B6 (sk) * 1995-05-05 2005-07-01 Unilever Nv Detergentná zmes obsahujúca subtilázový BLS309 variant
WO1998055634A1 (fr) * 1997-06-04 1998-12-10 The Procter & Gamble Company Enzymes proteases pour eliminer les souillures tenaces et/ou reduire la formation de taches et de films et compositions nettoyantes incorporant ces enzymes
ATE298791T1 (de) * 1997-11-21 2005-07-15 Novozymes As Protease-varianten und zusammensetzungen

Also Published As

Publication number Publication date
BR9916350A (pt) 2001-09-18
WO2000037624A1 (fr) 2000-06-29
KR20010093172A (ko) 2001-10-27
CN1333825A (zh) 2002-01-30
BRPI9916350B1 (pt) 2016-04-12
CN1302106C (zh) 2007-02-28
AU1772900A (en) 2000-07-12
KR100660799B1 (ko) 2006-12-26
AU773327B2 (en) 2004-05-20
JP4647788B2 (ja) 2011-03-09
CA2355579A1 (fr) 2000-06-29
JP2002533077A (ja) 2002-10-08

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Effective date: 20171220