CA2355920A1 - Procedes et kits permettant de determiner la stabilite de duplex d'acide nucleique - Google Patents

Procedes et kits permettant de determiner la stabilite de duplex d'acide nucleique Download PDF

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Publication number
CA2355920A1
CA2355920A1 CA002355920A CA2355920A CA2355920A1 CA 2355920 A1 CA2355920 A1 CA 2355920A1 CA 002355920 A CA002355920 A CA 002355920A CA 2355920 A CA2355920 A CA 2355920A CA 2355920 A1 CA2355920 A1 CA 2355920A1
Authority
CA
Canada
Prior art keywords
nucleic acid
duplex
strand
target
initial
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002355920A
Other languages
English (en)
Inventor
Kenneth J. Breslauer
Craig A. Gelfand
G. Eric Plum
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Rutgers State University of New Jersey
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2355920A1 publication Critical patent/CA2355920A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Cette invention concerne des procédés et des kits simples permettant de déterminer la stabilité thermodynamique de duplex d'acide nucléique et de polymorphismes polynucléotidiques simples via des équilibres concurrentiels.
CA002355920A 1998-12-23 1999-12-23 Procedes et kits permettant de determiner la stabilite de duplex d'acide nucleique Abandoned CA2355920A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US11373198P 1998-12-23 1998-12-23
US60/113,731 1998-12-23
US11990999P 1999-02-12 1999-02-12
US60/119,909 1999-02-12
PCT/US1999/030751 WO2000037686A1 (fr) 1998-12-23 1999-12-23 Procedes et kits permettant de determiner la stabilite de duplex d'acide nucleique

Publications (1)

Publication Number Publication Date
CA2355920A1 true CA2355920A1 (fr) 2000-06-29

Family

ID=26811403

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002355920A Abandoned CA2355920A1 (fr) 1998-12-23 1999-12-23 Procedes et kits permettant de determiner la stabilite de duplex d'acide nucleique

Country Status (4)

Country Link
EP (1) EP1155152A4 (fr)
AU (1) AU773480B2 (fr)
CA (1) CA2355920A1 (fr)
WO (1) WO2000037686A1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0129662D0 (en) 2001-12-11 2002-01-30 Whatman Internat Ltd Stabilisation of double-stranded nucleic acids

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5925517A (en) * 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
SE9502608D0 (sv) * 1995-07-14 1995-07-14 Pharmacia Biosensor Ab Method for nucleic acid senquencing
DE19782089T1 (de) * 1996-10-29 1999-12-23 Univ Nebraska At Lincoln Linco Verfahren zum Nachweisen von Punktmutationen in DNA unter Verwendung von Fluoreszenzenergieübergang

Also Published As

Publication number Publication date
EP1155152A4 (fr) 2003-05-02
AU773480B2 (en) 2004-05-27
EP1155152A1 (fr) 2001-11-21
AU2382100A (en) 2000-07-12
WO2000037686A1 (fr) 2000-06-29

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued