CA2362771A1 - Analyse genetique - Google Patents

Analyse genetique Download PDF

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Publication number
CA2362771A1
CA2362771A1 CA002362771A CA2362771A CA2362771A1 CA 2362771 A1 CA2362771 A1 CA 2362771A1 CA 002362771 A CA002362771 A CA 002362771A CA 2362771 A CA2362771 A CA 2362771A CA 2362771 A1 CA2362771 A1 CA 2362771A1
Authority
CA
Canada
Prior art keywords
dna
fragments
fragment
interest
restriction
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002362771A
Other languages
English (en)
Inventor
Michael Alan Reeve
Nicholas Ian Workman
Luis Martin-Parras
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
GE Healthcare UK Ltd
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2362771A1 publication Critical patent/CA2362771A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

L'invention concerne une technique d'analyse portant sur le génome complet. Cette technique, appelée déplétion en duplex à mésappariements parfaits inter-population, peut surmonter de nombreuses limitations des approches courantes basées sur les SNP et sur le déséquilibre de liaison au sein de populations isolées. La déplétion en duplex à mésappariements parfaits inter-population consiste à isoler un ou plusieurs fragments présentant des différences entre populations ou cellules <= touchées >= et <= non touchées >=. L'invention concerne une technique pratique - la matrice différentielle d'établissement de profils de sites de restriction terminaux (TRSPA) - pour l'analyse de tels fragments. L'invention concerne également un ADN de régulation interne totalement diagnostique permettant de déterminer sans ambiguïtés l'étendue et la nature exacte de toute digestion partielle pour la déplétion en duplex contenant des mésappariements parfaits inter-population et les restrictions TRSPA.
CA002362771A 1999-03-12 2000-03-10 Analyse genetique Abandoned CA2362771A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP99301933.0 1999-03-12
EP99301933 1999-03-12
PCT/GB2000/000916 WO2000055364A2 (fr) 1999-03-12 2000-03-10 Analyse genetique

Publications (1)

Publication Number Publication Date
CA2362771A1 true CA2362771A1 (fr) 2000-09-21

Family

ID=8241266

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002362771A Abandoned CA2362771A1 (fr) 1999-03-12 2000-03-10 Analyse genetique

Country Status (5)

Country Link
EP (1) EP1173609A2 (fr)
JP (1) JP2002538837A (fr)
AU (1) AU3178200A (fr)
CA (1) CA2362771A1 (fr)
WO (1) WO2000055364A2 (fr)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7141371B2 (en) 2002-09-06 2006-11-28 State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of The University Of Oregon Methods for detecting and localizing DNA mutations by microarray
DK1885882T3 (da) * 2005-05-10 2011-04-11 State Of Oregon Acting By & Through The State Board Of Higher Eduction On Behalf Of The University O Fremgangsmåder til kortlægning af polymorfier og polymorfi-mikroarray
WO2011053987A1 (fr) * 2009-11-02 2011-05-05 Nugen Technologies, Inc. Compositions et procédés de sélection et d'amplification de séquences d'acide nucléique ciblées
SG10201510189WA (en) 2011-10-19 2016-01-28 Nugen Technologies Inc Compositions And Methods For Directional Nucleic Acid Amplification And Sequencing
SG11201404243WA (en) 2012-01-26 2014-08-28 Nugen Technologies Inc Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
JP6181751B2 (ja) 2012-06-18 2017-08-16 ニューゲン テクノロジーズ， インコーポレイテッド 望まれない核酸配列のネガティブ選択のための組成物および方法
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
WO2014144092A1 (fr) 2013-03-15 2014-09-18 Nugen Technologies, Inc. Séquençage séquentiel
CA2929596C (fr) 2013-11-13 2022-07-05 Nugen Technologies, Inc. Compositions et procedes pour l'identification d'une lecture de sequencage en double
US9745614B2 (en) 2014-02-28 2017-08-29 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
CN107075581B (zh) 2014-08-06 2022-03-18 纽亘技术公司 由靶向测序进行数字测量
US12492430B2 (en) 2017-04-11 2025-12-09 Tecan Genomics, Inc. Library quantitation and qualification
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
EP3851542A1 (fr) 2020-01-20 2021-07-21 Tecan Genomics, Inc. Épuisement de séquences non informatives abondantes
CN113275053A (zh) 2020-02-03 2021-08-20 帝肯基因组学公司 试剂存储系统

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0226288A3 (fr) * 1985-10-09 1989-06-28 Collaborative Research Inc. Moyens et procédé de dosage de fibrose cystique à base d'une liaison génétique
US4771384A (en) * 1986-07-24 1988-09-13 Dnastar, Inc. System and method for fragmentation mapping
WO1989001526A1 (fr) * 1987-08-07 1989-02-23 Genelabs Incorporated Librairie et procede de clonage par coincidence
US5556750A (en) * 1989-05-12 1996-09-17 Duke University Methods and kits for fractionating a population of DNA molecules based on the presence or absence of a base-pair mismatch utilizing mismatch repair systems
US5316908A (en) * 1990-07-13 1994-05-31 Life Technologies, Inc. Size markers for electrophoretic analysis of DNA
AU3728093A (en) * 1992-02-19 1993-09-13 Public Health Research Institute Of The City Of New York, Inc., The Novel oligonucleotide arrays and their use for sorting, isolating, sequencing, and manipulating nucleic acids
WO1993022457A1 (fr) * 1992-04-24 1993-11-11 Massachusetts Institute Of Technology Criblage servant a detecter une variation genetique
US5376526A (en) * 1992-05-06 1994-12-27 The Board Of Trustees Of The Leland Stanford Junior University Genomic mismatch scanning
US5436142A (en) * 1992-11-12 1995-07-25 Cold Spring Harbor Laboratory Methods for producing probes capable of distingushing variant genomic sequences
EP0725821B1 (fr) * 1993-10-28 1998-07-15 Life Technologies, Inc. Echelle de reference d'acide nucleique pour l'estimation de la masse
US5710000A (en) * 1994-09-16 1998-01-20 Affymetrix, Inc. Capturing sequences adjacent to Type-IIs restriction sites for genomic library mapping
AU2264197A (en) * 1996-02-09 1997-08-28 Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The Restriction display (rd-pcr) of differentially expressed mrnas

Also Published As

Publication number Publication date
WO2000055364A3 (fr) 2001-10-11
WO2000055364A2 (fr) 2000-09-21
AU3178200A (en) 2000-10-04
JP2002538837A (ja) 2002-11-19
EP1173609A2 (fr) 2002-01-23

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