CA2365431A1 - Systeme de groupement universel de proteines (upa) - Google Patents
Systeme de groupement universel de proteines (upa) Download PDFInfo
- Publication number
- CA2365431A1 CA2365431A1 CA002365431A CA2365431A CA2365431A1 CA 2365431 A1 CA2365431 A1 CA 2365431A1 CA 002365431 A CA002365431 A CA 002365431A CA 2365431 A CA2365431 A CA 2365431A CA 2365431 A1 CA2365431 A1 CA 2365431A1
- Authority
- CA
- Canada
- Prior art keywords
- array
- probe
- protein
- binding
- polypeptide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 238000003498 protein array Methods 0.000 title claims abstract description 27
- 239000000523 sample Substances 0.000 claims abstract description 234
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 197
- 102000004196 processed proteins & peptides Human genes 0.000 claims abstract description 196
- 229920001184 polypeptide Polymers 0.000 claims abstract description 195
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 177
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 158
- 238000000034 method Methods 0.000 claims abstract description 79
- 238000002493 microarray Methods 0.000 claims abstract description 24
- 230000027455 binding Effects 0.000 claims description 83
- 230000003993 interaction Effects 0.000 claims description 52
- 238000003556 assay Methods 0.000 claims description 40
- 210000004027 cell Anatomy 0.000 claims description 38
- 239000000872 buffer Substances 0.000 claims description 32
- 102000039446 nucleic acids Human genes 0.000 claims description 26
- 108020004707 nucleic acids Proteins 0.000 claims description 26
- 150000007523 nucleic acids Chemical class 0.000 claims description 26
- 238000001514 detection method Methods 0.000 claims description 22
- 239000003446 ligand Substances 0.000 claims description 22
- 238000004458 analytical method Methods 0.000 claims description 20
- 201000010099 disease Diseases 0.000 claims description 11
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 11
- 239000000203 mixture Substances 0.000 claims description 11
- 239000007787 solid Substances 0.000 claims description 11
- 102100022846 Histone acetyltransferase KAT2B Human genes 0.000 claims description 10
- -1 PCAF-C Proteins 0.000 claims description 10
- 108010038912 Retinoid X Receptors Proteins 0.000 claims description 9
- 239000012634 fragment Substances 0.000 claims description 9
- 239000011521 glass Substances 0.000 claims description 9
- 101001047006 Homo sapiens Histone acetyltransferase KAT2B Proteins 0.000 claims description 8
- 108010083268 Transcription Factor TFIID Proteins 0.000 claims description 8
- 102000006290 Transcription Factor TFIID Human genes 0.000 claims description 8
- 108090000941 Transcription factor TFIIB Proteins 0.000 claims description 8
- 108091006108 transcriptional coactivators Proteins 0.000 claims description 8
- 102100033840 General transcription factor IIF subunit 1 Human genes 0.000 claims description 7
- 108010014677 transcription factor TFIIE Proteins 0.000 claims description 7
- 108010014678 transcription factor TFIIF Proteins 0.000 claims description 7
- 102100032863 General transcription factor IIH subunit 3 Human genes 0.000 claims description 6
- 230000006916 protein interaction Effects 0.000 claims description 6
- 101100098711 Drosophila melanogaster Taf1 gene Proteins 0.000 claims description 5
- 101000666405 Homo sapiens General transcription factor IIH subunit 1 Proteins 0.000 claims description 5
- 101000655398 Homo sapiens General transcription factor IIH subunit 2 Proteins 0.000 claims description 5
- 101000655391 Homo sapiens General transcription factor IIH subunit 3 Proteins 0.000 claims description 5
- 101000655406 Homo sapiens General transcription factor IIH subunit 4 Proteins 0.000 claims description 5
- 101000655402 Homo sapiens General transcription factor IIH subunit 5 Proteins 0.000 claims description 5
- 239000000020 Nitrocellulose Substances 0.000 claims description 5
- 230000002159 abnormal effect Effects 0.000 claims description 5
- 230000002163 immunogen Effects 0.000 claims description 5
- 229920001220 nitrocellulos Polymers 0.000 claims description 5
- 230000002103 transcriptional effect Effects 0.000 claims description 5
- 239000002033 PVDF binder Substances 0.000 claims description 4
- 239000000835 fiber Substances 0.000 claims description 4
- 229920002981 polyvinylidene fluoride Polymers 0.000 claims description 4
- 108091006106 transcriptional activators Proteins 0.000 claims description 4
- 239000004677 Nylon Substances 0.000 claims description 3
- 102100035593 POU domain, class 2, transcription factor 1 Human genes 0.000 claims description 3
- 101710084414 POU domain, class 2, transcription factor 1 Proteins 0.000 claims description 3
- 210000000349 chromosome Anatomy 0.000 claims description 3
- 230000007613 environmental effect Effects 0.000 claims description 3
- 229920001778 nylon Polymers 0.000 claims description 3
- 230000003698 anagen phase Effects 0.000 claims description 2
- 230000003612 virological effect Effects 0.000 claims description 2
- 101000870519 Rattus norvegicus Glutathione S-transferase alpha-4 Proteins 0.000 claims 2
- 102100040296 TATA-box-binding protein Human genes 0.000 claims 2
- 101710156592 Putative TATA-binding protein pB263R Proteins 0.000 claims 1
- 101710145783 TATA-box-binding protein Proteins 0.000 claims 1
- 108010083262 Transcription Factor TFIIA Proteins 0.000 claims 1
- 102000006289 Transcription Factor TFIIA Human genes 0.000 claims 1
- 102000004408 Transcription factor TFIIB Human genes 0.000 claims 1
- 239000002253 acid Substances 0.000 claims 1
- 230000009141 biological interaction Effects 0.000 claims 1
- 238000003491 array Methods 0.000 abstract description 63
- 238000002360 preparation method Methods 0.000 abstract description 11
- 238000004519 manufacturing process Methods 0.000 abstract description 4
- 235000018102 proteins Nutrition 0.000 description 130
- 102000053602 DNA Human genes 0.000 description 37
- 108020004414 DNA Proteins 0.000 description 37
- 239000000758 substrate Substances 0.000 description 25
- 238000013518 transcription Methods 0.000 description 18
- 230000035897 transcription Effects 0.000 description 18
- 101000663223 Mus musculus Serine/arginine-rich splicing factor 1 Proteins 0.000 description 17
- 229920002477 rna polymer Polymers 0.000 description 15
- 240000008791 Antiaris toxicaria Species 0.000 description 14
- 101001095095 Homo sapiens Proline-rich acidic protein 1 Proteins 0.000 description 14
- 102000006467 TATA-Box Binding Protein Human genes 0.000 description 14
- 108010044281 TATA-Box Binding Protein Proteins 0.000 description 14
- 238000002372 labelling Methods 0.000 description 14
- 102100036464 Activated RNA polymerase II transcriptional coactivator p15 Human genes 0.000 description 13
- 101710111216 Activated RNA polymerase II transcriptional coactivator p15 Proteins 0.000 description 13
- 241000894006 Bacteria Species 0.000 description 13
- 206010028980 Neoplasm Diseases 0.000 description 13
- 230000014509 gene expression Effects 0.000 description 13
- 102000005720 Glutathione transferase Human genes 0.000 description 12
- 108010070675 Glutathione transferase Proteins 0.000 description 12
- 239000012190 activator Substances 0.000 description 12
- 108700005092 MHC Class II Genes Proteins 0.000 description 11
- 238000011002 quantification Methods 0.000 description 11
- 108010038807 Oligopeptides Proteins 0.000 description 10
- 102000015636 Oligopeptides Human genes 0.000 description 10
- 108091023040 Transcription factor Proteins 0.000 description 10
- 201000011510 cancer Diseases 0.000 description 10
- 239000000463 material Substances 0.000 description 10
- 239000002773 nucleotide Substances 0.000 description 10
- 125000003729 nucleotide group Chemical group 0.000 description 10
- 230000008569 process Effects 0.000 description 10
- 102000040945 Transcription factor Human genes 0.000 description 9
- 102000004217 thyroid hormone receptors Human genes 0.000 description 9
- 108090000721 thyroid hormone receptors Proteins 0.000 description 9
- 102100021010 Nucleolin Human genes 0.000 description 8
- 108091034117 Oligonucleotide Proteins 0.000 description 8
- 108020004518 RNA Probes Proteins 0.000 description 8
- 239000003391 RNA probe Substances 0.000 description 8
- 102000034527 Retinoid X Receptors Human genes 0.000 description 8
- 230000000694 effects Effects 0.000 description 8
- 230000006870 function Effects 0.000 description 8
- 239000012528 membrane Substances 0.000 description 8
- 108010044762 nucleolin Proteins 0.000 description 8
- 150000003839 salts Chemical class 0.000 description 8
- 102100034701 Cilia- and flagella-associated protein 20 Human genes 0.000 description 7
- 230000004570 RNA-binding Effects 0.000 description 7
- 238000005516 engineering process Methods 0.000 description 7
- 239000000243 solution Substances 0.000 description 7
- 238000003786 synthesis reaction Methods 0.000 description 7
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 6
- 230000004568 DNA-binding Effects 0.000 description 6
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 6
- 108010033040 Histones Proteins 0.000 description 6
- 108010009460 RNA Polymerase II Proteins 0.000 description 6
- 102000009572 RNA Polymerase II Human genes 0.000 description 6
- 102000015097 RNA Splicing Factors Human genes 0.000 description 6
- 108010039259 RNA Splicing Factors Proteins 0.000 description 6
- 150000001413 amino acids Chemical class 0.000 description 6
- 230000015572 biosynthetic process Effects 0.000 description 6
- 210000004899 c-terminal region Anatomy 0.000 description 6
- 238000006243 chemical reaction Methods 0.000 description 6
- 230000003081 coactivator Effects 0.000 description 6
- 238000000338 in vitro Methods 0.000 description 6
- 108020004999 messenger RNA Proteins 0.000 description 6
- 229920000642 polymer Polymers 0.000 description 6
- 230000004850 protein–protein interaction Effects 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- 238000005406 washing Methods 0.000 description 6
- 102000052510 DNA-Binding Proteins Human genes 0.000 description 5
- 125000000539 amino acid group Chemical group 0.000 description 5
- 229940079593 drug Drugs 0.000 description 5
- 239000003814 drug Substances 0.000 description 5
- 230000007246 mechanism Effects 0.000 description 5
- 102000005962 receptors Human genes 0.000 description 5
- 108020003175 receptors Proteins 0.000 description 5
- 238000011160 research Methods 0.000 description 5
- 241000701447 unidentified baculovirus Species 0.000 description 5
- 108700020911 DNA-Binding Proteins Proteins 0.000 description 4
- 241000588724 Escherichia coli Species 0.000 description 4
- 101150062179 II gene Proteins 0.000 description 4
- 108091093037 Peptide nucleic acid Proteins 0.000 description 4
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 4
- 230000000875 corresponding effect Effects 0.000 description 4
- 230000005284 excitation Effects 0.000 description 4
- 230000005847 immunogenicity Effects 0.000 description 4
- 238000011534 incubation Methods 0.000 description 4
- 239000000178 monomer Substances 0.000 description 4
- 239000002547 new drug Substances 0.000 description 4
- 230000003287 optical effect Effects 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
- 238000002731 protein assay Methods 0.000 description 4
- 238000000746 purification Methods 0.000 description 4
- 230000002285 radioactive effect Effects 0.000 description 4
- 238000003196 serial analysis of gene expression Methods 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 239000013598 vector Substances 0.000 description 4
- 238000001262 western blot Methods 0.000 description 4
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 4
- 101150000874 11 gene Proteins 0.000 description 3
- 108090001008 Avidin Proteins 0.000 description 3
- 102000052052 Casein Kinase II Human genes 0.000 description 3
- 108010010919 Casein Kinase II Proteins 0.000 description 3
- 238000000018 DNA microarray Methods 0.000 description 3
- 239000003298 DNA probe Substances 0.000 description 3
- 102100037907 High mobility group protein B1 Human genes 0.000 description 3
- 102000006947 Histones Human genes 0.000 description 3
- 108091005461 Nucleic proteins Proteins 0.000 description 3
- 108091000080 Phosphotransferase Proteins 0.000 description 3
- 102220551501 Protein kinase C eta type_K29A_mutation Human genes 0.000 description 3
- 229920002684 Sepharose Polymers 0.000 description 3
- 238000000376 autoradiography Methods 0.000 description 3
- 229960002685 biotin Drugs 0.000 description 3
- 235000020958 biotin Nutrition 0.000 description 3
- 239000011616 biotin Substances 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 239000002299 complementary DNA Substances 0.000 description 3
- 238000012217 deletion Methods 0.000 description 3
- 230000037430 deletion Effects 0.000 description 3
- SLPJGDQJLTYWCI-UHFFFAOYSA-N dimethyl-(4,5,6,7-tetrabromo-1h-benzoimidazol-2-yl)-amine Chemical compound BrC1=C(Br)C(Br)=C2NC(N(C)C)=NC2=C1Br SLPJGDQJLTYWCI-UHFFFAOYSA-N 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 230000004927 fusion Effects 0.000 description 3
- 108020001507 fusion proteins Proteins 0.000 description 3
- 102000037865 fusion proteins Human genes 0.000 description 3
- 238000013090 high-throughput technology Methods 0.000 description 3
- 230000000670 limiting effect Effects 0.000 description 3
- 210000004962 mammalian cell Anatomy 0.000 description 3
- 235000013336 milk Nutrition 0.000 description 3
- 239000008267 milk Substances 0.000 description 3
- 210000004080 milk Anatomy 0.000 description 3
- 238000010369 molecular cloning Methods 0.000 description 3
- 230000035772 mutation Effects 0.000 description 3
- 210000004165 myocardium Anatomy 0.000 description 3
- 230000037361 pathway Effects 0.000 description 3
- 230000026731 phosphorylation Effects 0.000 description 3
- 238000006366 phosphorylation reaction Methods 0.000 description 3
- 102000020233 phosphotransferase Human genes 0.000 description 3
- 239000013612 plasmid Substances 0.000 description 3
- 102000040430 polynucleotide Human genes 0.000 description 3
- 108091033319 polynucleotide Proteins 0.000 description 3
- 239000002157 polynucleotide Substances 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 238000001742 protein purification Methods 0.000 description 3
- 230000001105 regulatory effect Effects 0.000 description 3
- 241000894007 species Species 0.000 description 3
- 210000001519 tissue Anatomy 0.000 description 3
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 2
- 239000004475 Arginine Substances 0.000 description 2
- 108091026890 Coding region Proteins 0.000 description 2
- 108020004705 Codon Proteins 0.000 description 2
- 108020003215 DNA Probes Proteins 0.000 description 2
- 108090000323 DNA Topoisomerases Proteins 0.000 description 2
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 2
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 2
- 102100023348 DNA-directed RNA polymerases I, II, and III subunit RPABC2 Human genes 0.000 description 2
- 101710153582 DNA-directed RNA polymerases I, II, and III subunit RPABC5 Proteins 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- 102000006580 General Transcription Factors Human genes 0.000 description 2
- 108010008945 General Transcription Factors Proteins 0.000 description 2
- 239000007995 HEPES buffer Substances 0.000 description 2
- 101710185235 High mobility group protein 1 Proteins 0.000 description 2
- 101710168537 High mobility group protein B1 Proteins 0.000 description 2
- 101710083341 Histone acetyltransferase KAT2B Proteins 0.000 description 2
- 101000686009 Homo sapiens DNA-directed RNA polymerases I, II, and III subunit RPABC2 Proteins 0.000 description 2
- 102220596833 Non-structural maintenance of chromosomes element 1 homolog_K41A_mutation Human genes 0.000 description 2
- 102000043276 Oncogene Human genes 0.000 description 2
- 108700020796 Oncogene Proteins 0.000 description 2
- 102000005613 PAX5 Transcription Factor Human genes 0.000 description 2
- 108010045055 PAX5 Transcription Factor Proteins 0.000 description 2
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 2
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 239000011324 bead Substances 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 239000004202 carbamide Substances 0.000 description 2
- 230000001413 cellular effect Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000007795 chemical reaction product Substances 0.000 description 2
- 238000004891 communication Methods 0.000 description 2
- 230000006854 communication Effects 0.000 description 2
- 230000009137 competitive binding Effects 0.000 description 2
- 230000000295 complement effect Effects 0.000 description 2
- 238000004132 cross linking Methods 0.000 description 2
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 2
- 238000013500 data storage Methods 0.000 description 2
- 238000000151 deposition Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 238000006911 enzymatic reaction Methods 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 235000013861 fat-free Nutrition 0.000 description 2
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 2
- 239000005556 hormone Substances 0.000 description 2
- 229940088597 hormone Drugs 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 229910052500 inorganic mineral Inorganic materials 0.000 description 2
- 239000011707 mineral Substances 0.000 description 2
- 239000013642 negative control Substances 0.000 description 2
- 210000003463 organelle Anatomy 0.000 description 2
- YBYRMVIVWMBXKQ-UHFFFAOYSA-N phenylmethanesulfonyl fluoride Chemical compound FS(=O)(=O)CC1=CC=CC=C1 YBYRMVIVWMBXKQ-UHFFFAOYSA-N 0.000 description 2
- 229920005597 polymer membrane Polymers 0.000 description 2
- 238000002203 pretreatment Methods 0.000 description 2
- 238000010379 pull-down assay Methods 0.000 description 2
- 230000005855 radiation Effects 0.000 description 2
- 108020004418 ribosomal RNA Proteins 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 230000019491 signal transduction Effects 0.000 description 2
- 230000009870 specific binding Effects 0.000 description 2
- 230000000087 stabilizing effect Effects 0.000 description 2
- 238000002560 therapeutic procedure Methods 0.000 description 2
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 description 2
- 239000003053 toxin Substances 0.000 description 2
- 231100000765 toxin Toxicity 0.000 description 2
- 108700012359 toxins Proteins 0.000 description 2
- 230000007704 transition Effects 0.000 description 2
- 230000014616 translation Effects 0.000 description 2
- 238000010396 two-hybrid screening Methods 0.000 description 2
- 241000701161 unidentified adenovirus Species 0.000 description 2
- 239000002435 venom Substances 0.000 description 2
- 231100000611 venom Toxicity 0.000 description 2
- 210000001048 venom Anatomy 0.000 description 2
- 102220497337 14-3-3 protein zeta/delta_R59A_mutation Human genes 0.000 description 1
- ASJSAQIRZKANQN-CRCLSJGQSA-N 2-deoxy-D-ribose Chemical group OC[C@@H](O)[C@@H](O)CC=O ASJSAQIRZKANQN-CRCLSJGQSA-N 0.000 description 1
- 108091006112 ATPases Proteins 0.000 description 1
- 101710159080 Aconitate hydratase A Proteins 0.000 description 1
- 101710159078 Aconitate hydratase B Proteins 0.000 description 1
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
- 229930024421 Adenine Natural products 0.000 description 1
- 102000057290 Adenosine Triphosphatases Human genes 0.000 description 1
- 241000219194 Arabidopsis Species 0.000 description 1
- 101100178203 Arabidopsis thaliana HMGB3 gene Proteins 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 108010034798 CDC2 Protein Kinase Proteins 0.000 description 1
- 102000009728 CDC2 Protein Kinase Human genes 0.000 description 1
- 241000244203 Caenorhabditis elegans Species 0.000 description 1
- 208000005623 Carcinogenesis Diseases 0.000 description 1
- 208000017667 Chronic Disease Diseases 0.000 description 1
- 102000008130 Cyclic AMP-Dependent Protein Kinases Human genes 0.000 description 1
- 108010049894 Cyclic AMP-Dependent Protein Kinases Proteins 0.000 description 1
- 102000004594 DNA Polymerase I Human genes 0.000 description 1
- 108010017826 DNA Polymerase I Proteins 0.000 description 1
- 102000003915 DNA Topoisomerases Human genes 0.000 description 1
- 238000001712 DNA sequencing Methods 0.000 description 1
- 102100024607 DNA topoisomerase 1 Human genes 0.000 description 1
- 101710096438 DNA-binding protein Proteins 0.000 description 1
- 241000255601 Drosophila melanogaster Species 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 108091092566 Extrachromosomal DNA Proteins 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 238000010631 GST assay Methods 0.000 description 1
- 229910005540 GaP Inorganic materials 0.000 description 1
- 108010001515 Galectin 4 Proteins 0.000 description 1
- 102100039556 Galectin-4 Human genes 0.000 description 1
- 229910001218 Gallium arsenide Inorganic materials 0.000 description 1
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 1
- 241000447439 Gerres Species 0.000 description 1
- 101150091750 HMG1 gene Proteins 0.000 description 1
- 108700010013 HMGB1 Proteins 0.000 description 1
- 101150021904 HMGB1 gene Proteins 0.000 description 1
- 101710105440 High mobility group protein I Proteins 0.000 description 1
- 208000026350 Inborn Genetic disease Diseases 0.000 description 1
- 108010070716 Intercellular Signaling Peptides and Proteins Proteins 0.000 description 1
- 102000005755 Intercellular Signaling Peptides and Proteins Human genes 0.000 description 1
- 102000018697 Membrane Proteins Human genes 0.000 description 1
- 108010052285 Membrane Proteins Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 108020004711 Nucleic Acid Probes Proteins 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 102000003840 Opioid Receptors Human genes 0.000 description 1
- 108090000137 Opioid Receptors Proteins 0.000 description 1
- 108010021757 Polynucleotide 5'-Hydroxyl-Kinase Proteins 0.000 description 1
- 102000008422 Polynucleotide 5'-hydroxyl-kinase Human genes 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 102220551503 Protein kinase C eta type_R27A_mutation Human genes 0.000 description 1
- 108010065868 RNA polymerase SP6 Proteins 0.000 description 1
- 102000044126 RNA-Binding Proteins Human genes 0.000 description 1
- 101710105008 RNA-binding protein Proteins 0.000 description 1
- 108091028664 Ribonucleotide Proteins 0.000 description 1
- 108091078180 SR family Proteins 0.000 description 1
- 241000235070 Saccharomyces Species 0.000 description 1
- 101710106714 Shutoff protein Proteins 0.000 description 1
- 108020004682 Single-Stranded DNA Proteins 0.000 description 1
- 108700026226 TATA Box Proteins 0.000 description 1
- 108010091120 TATA-Binding Protein Associated Factors Proteins 0.000 description 1
- 102000018068 TATA-Binding Protein Associated Factors Human genes 0.000 description 1
- AUYYCJSJGJYCDS-LBPRGKRZSA-N Thyrolar Chemical compound IC1=CC(C[C@H](N)C(O)=O)=CC(I)=C1OC1=CC=C(O)C(I)=C1 AUYYCJSJGJYCDS-LBPRGKRZSA-N 0.000 description 1
- 108010083256 Transcription Factor TFIIH Proteins 0.000 description 1
- 102000044209 Tumor Suppressor Genes Human genes 0.000 description 1
- 108700025716 Tumor Suppressor Genes Proteins 0.000 description 1
- 102000001742 Tumor Suppressor Proteins Human genes 0.000 description 1
- 108010040002 Tumor Suppressor Proteins Proteins 0.000 description 1
- 108700005077 Viral Genes Proteins 0.000 description 1
- 241000269368 Xenopus laevis Species 0.000 description 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 239000008186 active pharmaceutical agent Substances 0.000 description 1
- 229960000643 adenine Drugs 0.000 description 1
- 150000001408 amides Chemical class 0.000 description 1
- 235000021120 animal protein Nutrition 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 230000009830 antibody antigen interaction Effects 0.000 description 1
- 239000002246 antineoplastic agent Substances 0.000 description 1
- 229940041181 antineoplastic drug Drugs 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 210000003719 b-lymphocyte Anatomy 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008238 biochemical pathway Effects 0.000 description 1
- 238000005842 biochemical reaction Methods 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000008512 biological response Effects 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 230000036952 cancer formation Effects 0.000 description 1
- 231100000504 carcinogenesis Toxicity 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 230000033077 cellular process Effects 0.000 description 1
- 230000002759 chromosomal effect Effects 0.000 description 1
- 238000000749 co-immunoprecipitation Methods 0.000 description 1
- 238000011490 co-immunoprecipitation assay Methods 0.000 description 1
- 238000012875 competitive assay Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 229940104302 cytosine Drugs 0.000 description 1
- RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 239000005547 deoxyribonucleotide Substances 0.000 description 1
- 125000002637 deoxyribonucleotide group Chemical group 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 230000005670 electromagnetic radiation Effects 0.000 description 1
- 238000001952 enzyme assay Methods 0.000 description 1
- 210000003527 eukaryotic cell Anatomy 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 239000013604 expression vector Substances 0.000 description 1
- 208000016361 genetic disease Diseases 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 229910052732 germanium Inorganic materials 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 239000012145 high-salt buffer Substances 0.000 description 1
- 108091008039 hormone receptors Proteins 0.000 description 1
- 210000003917 human chromosome Anatomy 0.000 description 1
- 238000010191 image analysis Methods 0.000 description 1
- 238000003119 immunoblot Methods 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 210000003000 inclusion body Anatomy 0.000 description 1
- 230000002458 infectious effect Effects 0.000 description 1
- 238000012482 interaction analysis Methods 0.000 description 1
- 230000009878 intermolecular interaction Effects 0.000 description 1
- 230000008863 intramolecular interaction Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 101150066555 lacZ gene Proteins 0.000 description 1
- 238000004020 luminiscence type Methods 0.000 description 1
- 239000008176 lyophilized powder Substances 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 229910021645 metal ion Inorganic materials 0.000 description 1
- MYWUZJCMWCOHBA-VIFPVBQESA-N methamphetamine Chemical compound CN[C@@H](C)CC1=CC=CC=C1 MYWUZJCMWCOHBA-VIFPVBQESA-N 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000011278 mitosis Effects 0.000 description 1
- 230000009149 molecular binding Effects 0.000 description 1
- 238000000329 molecular dynamics simulation Methods 0.000 description 1
- 239000002853 nucleic acid probe Substances 0.000 description 1
- 229940046166 oligodeoxynucleotide Drugs 0.000 description 1
- 238000002966 oligonucleotide array Methods 0.000 description 1
- 244000045947 parasite Species 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000008506 pathogenesis Effects 0.000 description 1
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 description 1
- 238000000206 photolithography Methods 0.000 description 1
- 210000002826 placenta Anatomy 0.000 description 1
- 239000013600 plasmid vector Substances 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920000515 polycarbonate Polymers 0.000 description 1
- 239000004417 polycarbonate Substances 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 229920001343 polytetrafluoroethylene Polymers 0.000 description 1
- 239000004810 polytetrafluoroethylene Substances 0.000 description 1
- 238000003259 recombinant expression Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 230000026267 regulation of growth Effects 0.000 description 1
- 238000004153 renaturation Methods 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 230000000241 respiratory effect Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 239000002336 ribonucleotide Substances 0.000 description 1
- 125000002652 ribonucleotide group Chemical group 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- LIVNPJMFVYWSIS-UHFFFAOYSA-N silicon monoxide Inorganic materials [Si-]#[O+] LIVNPJMFVYWSIS-UHFFFAOYSA-N 0.000 description 1
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 1
- 239000011343 solid material Substances 0.000 description 1
- 108020001568 subdomains Proteins 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- RYYWUUFWQRZTIU-UHFFFAOYSA-K thiophosphate Chemical compound [O-]P([O-])([O-])=S RYYWUUFWQRZTIU-UHFFFAOYSA-K 0.000 description 1
- 229940113082 thymine Drugs 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 230000009261 transgenic effect Effects 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 102000035160 transmembrane proteins Human genes 0.000 description 1
- 108091005703 transmembrane proteins Proteins 0.000 description 1
- 239000000225 tumor suppressor protein Substances 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 239000011534 wash buffer Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54306—Solid-phase reaction mechanisms
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
- B01J2219/0061—The surface being organic
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00639—Making arrays on substantially continuous surfaces the compounds being trapped in or bound to a porous medium
- B01J2219/00641—Making arrays on substantially continuous surfaces the compounds being trapped in or bound to a porous medium the porous medium being continuous, e.g. porous oxide substrates
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00659—Two-dimensional arrays
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00718—Type of compounds synthesised
- B01J2219/0072—Organic compounds
- B01J2219/00725—Peptides
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/04—Libraries containing only organic compounds
- C40B40/10—Libraries containing peptides or polypeptides, or derivatives thereof
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Cell Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Pathology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Peptides Or Proteins (AREA)
Abstract
L'invention a trait à des groupements ordonnés de molécules, par exemple de polypeptides ou de protéines. Ces groupements, appelés groupements universels de protéines, sont décrits dans des formats de macrogroupements et de microgroupements. Des procédés de production et d'utilisation de ces groupements sont également décrits. L'invention concerne également des trousses utiles pour ces groupements et pour la préparation de sondes s'utilisant avec ceux-ci.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US12358699P | 1999-03-10 | 1999-03-10 | |
| US60/123,586 | 1999-03-10 | ||
| PCT/US2000/006244 WO2000054046A2 (fr) | 1999-03-10 | 2000-03-10 | Systeme de groupement universel de proteines (upa) |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2365431A1 true CA2365431A1 (fr) | 2000-09-14 |
Family
ID=22409568
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002365431A Abandoned CA2365431A1 (fr) | 1999-03-10 | 2000-03-10 | Systeme de groupement universel de proteines (upa) |
Country Status (4)
| Country | Link |
|---|---|
| EP (1) | EP1159615A2 (fr) |
| AU (1) | AU773291B2 (fr) |
| CA (1) | CA2365431A1 (fr) |
| WO (1) | WO2000054046A2 (fr) |
Families Citing this family (61)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6576478B1 (en) | 1998-07-14 | 2003-06-10 | Zyomyx, Inc. | Microdevices for high-throughput screening of biomolecules |
| US6406921B1 (en) | 1998-07-14 | 2002-06-18 | Zyomyx, Incorporated | Protein arrays for high-throughput screening |
| US6897073B2 (en) | 1998-07-14 | 2005-05-24 | Zyomyx, Inc. | Non-specific binding resistant protein arrays and methods for making the same |
| US6780582B1 (en) | 1998-07-14 | 2004-08-24 | Zyomyx, Inc. | Arrays of protein-capture agents and methods of use thereof |
| US6682942B1 (en) | 1998-07-14 | 2004-01-27 | Zyomyx, Inc. | Microdevices for screening biomolecules |
| AU2001284899A1 (en) * | 2000-08-11 | 2002-02-25 | Qianjin Hu | Methods and universal monoclonal antibody array |
| AUPR005600A0 (en) * | 2000-09-12 | 2000-10-05 | University Of Sydney, The | Diagnostic assay |
| GB0022978D0 (en) * | 2000-09-19 | 2000-11-01 | Oxford Glycosciences Uk Ltd | Detection of peptides |
| US7354721B2 (en) | 2000-09-22 | 2008-04-08 | Clontech Laboratories, Inc. | Highly sensitive proteomic analysis methods, and kits and systems for practicing the same |
| DE10054055A1 (de) | 2000-10-31 | 2002-05-23 | Nmi Univ Tuebingen | Verfahren zur Analyse von Proteinen |
| EP1397679B1 (fr) | 2000-11-27 | 2010-01-20 | Intelligent Medical Devices LLC | Dispositifs et procedes de diagnostic cliniquement intelligents |
| EP1360490B1 (fr) | 2001-01-23 | 2011-12-21 | President and Fellows of Harvard College | Reseaux de proteines a acide nucleique programmable |
| US7332286B2 (en) | 2001-02-02 | 2008-02-19 | University Of Pennsylvania | Peptide or protein microassay method and apparatus |
| DE10118774A1 (de) * | 2001-04-17 | 2002-10-31 | Jerini Ag | Verfahren zur Bestimmung der Substratspezifität einer enzymatischen Aktivität und Vorrichtung hierzu |
| ATE380342T1 (de) | 2001-05-07 | 2007-12-15 | Hipep Lab | Peptidimmobilisierte grundplatte und vefahren zum testen eines zielproteins unter verwendung derselben |
| US7981842B2 (en) * | 2001-06-08 | 2011-07-19 | Panomics, Inc. | Method for detecting transcription factor-protein interactions |
| EP1279963A1 (fr) * | 2001-07-27 | 2003-01-29 | Université de Nantes | Procédé de criblage de cibles des protéines utilisant des colorants fluorescents dans le proche infrarouge |
| WO2003023360A2 (fr) | 2001-09-10 | 2003-03-20 | Meso Scale Technologies, Llc | Procedes et appareils permettant de realiser de multiples mesures sur un echantillon |
| EP1451579A4 (fr) * | 2001-11-19 | 2005-12-28 | Protometrix Inc | Procede d'utilisation d'une proteine non anticorps permettant de detecter et de mesurer un analyte |
| GB0200617D0 (en) * | 2002-01-11 | 2002-02-27 | Oxford Glycosciences Uk Ltd | Characterisation of analytes |
| GB0205910D0 (en) * | 2002-03-13 | 2002-04-24 | Sense Proteomic Ltd | Arrays and methods |
| US7618788B2 (en) | 2002-05-10 | 2009-11-17 | Millipore Corporation | Proteome epitope tags and methods of use thereof in protein modification analysis |
| US7460960B2 (en) | 2002-05-10 | 2008-12-02 | Epitome Biosystems, Inc. | Proteome epitope tags and methods of use thereof in protein modification analysis |
| GB2402131A (en) * | 2003-03-13 | 2004-12-01 | Sense Proteomic Ltd | Early entry |
| US20040209262A1 (en) * | 2003-04-21 | 2004-10-21 | Bass Jay K. | Biopolymeric arrays comprising test probes for two or more different species and methods for using the same |
| US20040248323A1 (en) | 2003-06-09 | 2004-12-09 | Protometrix, Inc. | Methods for conducting assays for enzyme activity on protein microarrays |
| EP1711819A4 (fr) | 2003-12-15 | 2008-04-16 | Univ Pennsylvania Ct For Techn | Procede et dispositifs pour la realisation de reactions sur une plaque cible pour la spectrometrie de masse de desorption-ionisation par impact laser assistee par matrice |
| KR100690948B1 (ko) | 2004-06-17 | 2007-03-09 | 한국과학기술원 | 대장균의 외막 단백질을 이용한 목적 단백질의 미생물표면발현 방법 |
| CN1312293C (zh) * | 2004-11-08 | 2007-04-25 | 博奥生物有限公司 | 一种高通量生物芯片及其应用 |
| KR100784261B1 (ko) | 2006-01-02 | 2007-12-11 | 한국과학기술원 | 탄저균의 포자외막 단백질을 이용한 목적단백질의 미생물표면발현방법 |
| US7855057B2 (en) | 2006-03-23 | 2010-12-21 | Millipore Corporation | Protein splice variant/isoform discrimination and quantitative measurements thereof |
| US8178316B2 (en) | 2006-06-29 | 2012-05-15 | President And Fellows Of Harvard College | Evaluating proteins |
| EP3796002B1 (fr) | 2006-07-14 | 2023-11-22 | The Regents of The University of California | Biomarqueurs du cancer et leurs procédés d'utilisation |
| EP2408905B1 (fr) | 2009-03-16 | 2017-05-10 | Pangu Biopharma Limited | Compositions et procedes comprenant des variants d'epissage d'histidyl-arnt synthetase presentant des activites biologiques non canoniques |
| CN102449143B (zh) | 2009-03-31 | 2017-11-14 | Atyr医药公司 | 包含具有非常规生物活性的天冬氨酰‑tRNA合成酶的组合物和方法 |
| US8980253B2 (en) | 2010-04-26 | 2015-03-17 | Atyr Pharma, Inc. | Innovative discovery of therapeutic, diagnostic, and antibody compositions related to protein fragments of cysteinyl-tRNA synthetase |
| CA2797362C (fr) | 2010-04-27 | 2020-12-08 | Atyr Pharma, Inc. | Decouverte innovante de compositions therapeutiques, de diagnostic et d'anticorps se rapportant a des fragments proteiques d'isoleucyl arnt synthetases |
| WO2011139721A1 (fr) | 2010-04-27 | 2011-11-10 | The Regents Of The University Of California | Biomarqueurs de cancer et leurs procédés d'utilisation |
| WO2011139853A2 (fr) | 2010-04-28 | 2011-11-10 | Atyr Pharma, Inc. | Compositions thérapeutiques, diagnostiques et d'anticorps à base de fragments de protéines d'aminoacyl-arnt synthétases |
| CA2797393C (fr) | 2010-04-29 | 2020-03-10 | Pangu Biopharma Limited | Decouverte innovatrice de compositions therapeutiques, diagnostiques, et d'anticorps associees aux fragments proteiques des valyle arnt synthesases |
| CA2800557A1 (fr) | 2010-04-29 | 2011-11-03 | Medical Prognosis Institute A/S | Methodes et dispositifs permettant de predire l'efficacite d'un traitement |
| CA2797374C (fr) | 2010-04-29 | 2021-02-16 | Pangu Biopharma Limited | Decouverte innovante de compositions therapeutiques, diagnostiques et a based' anticorps associees a des fragments proteiques d'asparaginyl-arnt-synthetases |
| CN103140233B (zh) | 2010-05-03 | 2017-04-05 | Atyr 医药公司 | 与甲硫氨酰‑tRNA合成酶的蛋白片段相关的治疗、诊断和抗体组合物的发现 |
| US8961961B2 (en) | 2010-05-03 | 2015-02-24 | a Tyr Pharma, Inc. | Innovative discovery of therapeutic, diagnostic, and antibody compositions related protein fragments of arginyl-tRNA synthetases |
| CN103096912A (zh) | 2010-05-03 | 2013-05-08 | Atyr医药公司 | 与苯丙氨酰-α-tRNA合成酶的蛋白片段相关的治疗、诊断和抗体组合物的创新发现 |
| JP6008844B2 (ja) | 2010-05-04 | 2016-10-19 | エータイアー ファーマ， インコーポレイテッド | ｐ３８ＭＵＬＴＩ−ｔＲＮＡ合成酵素複合体のタンパク質フラグメントに関連した治療用、診断用および抗体組成物の革新的発見 |
| EP2568996B1 (fr) | 2010-05-14 | 2017-10-04 | aTyr Pharma, Inc. | Compositions thérapeutique, diagnostique et à base d'anticorps contenant des fragments protéiques de phénylalanyl-bêta-arnt synthétases |
| CN103096914B (zh) | 2010-05-17 | 2015-08-12 | Atyr医药公司 | 与亮氨酰-tRNA合成酶的蛋白片段相关的治疗、诊断和抗体组合物的创新发现 |
| CA2800375C (fr) | 2010-05-27 | 2021-03-09 | Atyr Pharma, Inc. | Decouverte innovante de compositions therapeutiques, de diagnostic et d'anticorps liees a fragments proteiques de glutaminyl-arnt synthetases |
| EP2575857B1 (fr) | 2010-06-01 | 2018-01-24 | aTyr Pharma, Inc. | Découverte innovante de compositions thérapeutiques, diagnostiques, et d'anticorps associés à des fragments de protéine de lysyl-tarn synthétases |
| EP2593125B1 (fr) | 2010-07-12 | 2017-11-01 | aTyr Pharma, Inc. | DÉCOUVERTE INNOVANTE DE COMPOSITIONS THÉRAPEUTIQUES, DE DIAGNOSTIC ET D'ANTICORPS SE RAPPORTANT À DES FRAGMENTS PROTÉIQUES DE GLYCYL-ARNt SYNTHÉTASES |
| AU2011293294B2 (en) | 2010-08-25 | 2016-03-24 | Pangu Biopharma Limited | Innovative discovery of therapeutic, diagnostic, and antibody compositions related to protein fragments of Tyrosyl-tRNA synthetases |
| CA2836836A1 (fr) | 2011-06-01 | 2012-12-06 | Medical Prognosis Institute A/S | Procedes et dispositifs pour le pronostic d'une rechute du cancer |
| MX356107B (es) | 2012-02-16 | 2018-05-15 | Atyr Pharma Inc | Histidil-arnt sintetasas para tratar enfermedades autoinmunes e inflamatorias. |
| US10260103B2 (en) | 2012-11-27 | 2019-04-16 | Pontificia Universidad Catolica De Chile | Compositions and methods for diagnosing thyroid tumors |
| WO2014195032A1 (fr) | 2013-06-07 | 2014-12-11 | Medical Prognosis Institute A/S | Procédés et dispositifs pour prédire une efficacité de traitement de fulvestrant chez des patients atteints de cancer |
| CA2954764A1 (fr) | 2014-07-15 | 2016-01-21 | Ontario Institute For Cancer Research | Procedes et dispositifs permettant de predire l'efficacite d'un traitement a l'anthracycline |
| WO2016090323A1 (fr) | 2014-12-05 | 2016-06-09 | Prelude, Inc. | Récurrence de carcinome canalaire in situ (dcis) et cancer du sein invasif |
| CN108348547B (zh) | 2015-08-28 | 2023-09-22 | 纽约市哥伦比亚大学信托人 | 用于匹配肿瘤学特征的系统和方法 |
| EP3341498B1 (fr) | 2015-08-28 | 2022-03-30 | The Trustees of Columbia University in the City of New York | Interférence virtuelle de l'activité protéique par analyse d'enrichissement d'un régulon |
| WO2020056338A1 (fr) | 2018-09-14 | 2020-03-19 | Prelude Corporation | Méthode de sélection pour le traitement d'individus présentant un risque de cancer du sein invasif |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0063810B1 (fr) * | 1981-04-29 | 1986-03-05 | Ciba-Geigy Ag | Dispositif et trousses pour les analyses immunologiques |
| JPH1025299A (ja) * | 1996-07-12 | 1998-01-27 | Nec Corp | 整列ペプチド、及びそれを用いたタンパク質の結合・相互作用部位検出方法 |
| US6406921B1 (en) * | 1998-07-14 | 2002-06-18 | Zyomyx, Incorporated | Protein arrays for high-throughput screening |
-
2000
- 2000-03-10 CA CA002365431A patent/CA2365431A1/fr not_active Abandoned
- 2000-03-10 AU AU41705/00A patent/AU773291B2/en not_active Ceased
- 2000-03-10 EP EP00921374A patent/EP1159615A2/fr not_active Withdrawn
- 2000-03-10 WO PCT/US2000/006244 patent/WO2000054046A2/fr not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| WO2000054046A2 (fr) | 2000-09-14 |
| WO2000054046A3 (fr) | 2000-12-21 |
| EP1159615A2 (fr) | 2001-12-05 |
| AU773291B2 (en) | 2004-05-20 |
| AU4170500A (en) | 2000-09-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| AU773291B2 (en) | UPA, a universal protein array system | |
| US20030087298A1 (en) | Detection of hybridization on oligonucleotide microarray through covalently labeling microarray probe | |
| US6534271B2 (en) | Cloning and copying on surfaces | |
| US6448387B1 (en) | Polymeric arrays adapted for high expressing polynucleotides | |
| EP1356860A2 (fr) | Procédé de controle de la qualité d'un microréseau d'ADN | |
| WO1991000288A1 (fr) | Sonde a acide nucleique hydrophobe | |
| JP4425640B2 (ja) | Ｄｎａ−結合タンパク質の検出法 | |
| US7217518B2 (en) | Fluorescence polarization assay | |
| AU2001247634A1 (en) | Combined polynucleotide sequences as discrete assay endpoints | |
| WO2008003100A2 (fr) | Fabrication et utilisation de molécules de surface à différentes densités | |
| JP2006518183A (ja) | コード核酸担体 | |
| US20030044320A1 (en) | High throughput screening micro array platform | |
| US20070160998A1 (en) | Device for analyzing an interaction between target and probe molecules | |
| CN101663406A (zh) | 用于得到未知生物分子和单链核酸的结合谱的核酸芯片及其制备方法和利用该核酸芯片分析未知生物分子的方法 | |
| JP2002538836A (ja) | 遺伝子発現の変化の分析 | |
| Venton et al. | Screening combinatorial libraries | |
| KR20040035248A (ko) | 단일가닥핵산 어레이를 이용한 특정물질 동정 및 분석방법및 대장균에 대한 핵산 리간드 | |
| US20060014161A1 (en) | Combination comprising biochip and optical detection device | |
| CN101939448A (zh) | 与多重基因组获得和丢失分析相关的方法和组合物 | |
| US20060084101A1 (en) | Two-color chemiluminescent microarray system | |
| JP3944576B2 (ja) | マイクロアレイを用いたアプタマーの取得方法 | |
| Janitz | Assigning functions to genes—the main challenge of the post-genomics era | |
| US20050239078A1 (en) | Sequence tag microarray and method for detection of multiple proteins through DNA methods | |
| JP2001255327A (ja) | 多孔質支持体と遅延蛍光を用いる物質の検出及び／又は定量法 | |
| KR101132052B1 (ko) | Ｄｎａ 염기 서열에 특이적으로 결합하는 단백질을 이용한ｄｎａ선별 검출방법 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| FZDE | Discontinued |