CA2365594A1 - Methode de production microbiologique d'.alpha.-l-aspartyl-l-phenylalanine - Google Patents

Methode de production microbiologique d'.alpha.-l-aspartyl-l-phenylalanine Download PDF

Info

Publication number
CA2365594A1
CA2365594A1 CA002365594A CA2365594A CA2365594A1 CA 2365594 A1 CA2365594 A1 CA 2365594A1 CA 002365594 A CA002365594 A CA 002365594A CA 2365594 A CA2365594 A CA 2365594A CA 2365594 A1 CA2365594 A1 CA 2365594A1
Authority
CA
Canada
Prior art keywords
asp
phe
domain
synthetase
dipeptide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002365594A
Other languages
English (en)
Inventor
Sasha Doekel
Mohamed Abdalla Marahiel
Peter Jan Leonard Mario Quaedflieg
Theodorus Sonke
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Holland Sweetener Co VOF
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from EP99203518A external-priority patent/EP1096011A1/fr
Application filed by Holland Sweetener Co VOF filed Critical Holland Sweetener Co VOF
Publication of CA2365594A1 publication Critical patent/CA2365594A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La présente invention concerne une nouvelle méthode microbiologique de production d'.alpha.-L-aspartyl-L-phénylalanine (Asp-Phe) à partir des substrats acide L-aspartique (L-Asp) et L-phénylalanine (L-Phe) dans laquelle les substrats sont mis en contact, en la présence d'ATP, avec une synthétase dipeptidique non ribosomique contenant deux modules minimaux reliés par un domaine de condensation dans lequel les modules C-terminaux N-resp reconnaissent L-Asp et L-Phe respectivement, et le dernier module est lié de manière covalente au niveau de son extrémité N-terminale au domaine de condensation, chacun de ces modules minimaux étant composés d'un domaine d'adénylation et d'un domaine de thiolation contenant un cofacteur 4'-phosphopantétheinyle, et le Asp-Phe formé est récupéré. La présente invention concerne également de nouveaux fragments d'ADN ou une combinaison de fragemts d'ADN codant une nouvelle synthétase dipeptidique Asp-Phe, des micro-organismes contenant ces fragments d'ADN, ainsi que les nouvelles synthétases dipeptidiques Asp-Phe elles-mêmes.
CA002365594A 1999-03-29 2000-03-28 Methode de production microbiologique d'.alpha.-l-aspartyl-l-phenylalanine Abandoned CA2365594A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP99200954 1999-03-29
EP99200954.8 1999-10-26
EP99203518.8 1999-10-26
EP99203518A EP1096011A1 (fr) 1999-05-10 1999-10-26 Procédé microbiologique pour la préparation de l'alpha-L-aspartyl-L-phénylalanine
PCT/NL2000/000206 WO2000058478A1 (fr) 1999-03-29 2000-03-28 METHODE DE PRODUCTION MICROBIOLOGIQUE D'α-L-ASPARTYL-L-PHENYLALANINE

Publications (1)

Publication Number Publication Date
CA2365594A1 true CA2365594A1 (fr) 2000-10-05

Family

ID=26153289

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002365594A Abandoned CA2365594A1 (fr) 1999-03-29 2000-03-28 Methode de production microbiologique d'.alpha.-l-aspartyl-l-phenylalanine

Country Status (9)

Country Link
EP (1) EP1165799A1 (fr)
JP (1) JP2002539835A (fr)
KR (1) KR20010112931A (fr)
CN (1) CN1353757A (fr)
AU (1) AU3843900A (fr)
BR (1) BR0010398A (fr)
CA (1) CA2365594A1 (fr)
EA (1) EA200101015A1 (fr)
MX (1) MXPA01009875A (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100362109C (zh) * 2003-01-24 2008-01-16 味之素株式会社 α-L-天冬氨酰-L-苯丙氨酸-β-酯的生产方法和α-L-天冬氨酰-L-苯丙氨酸-α-甲酯的生产方法
JP4500978B2 (ja) * 2003-01-24 2010-07-14 味の素株式会社 α−Ｌ−アスパルチル−Ｌ−フェニルアラニン−β−エステルの製造方法およびα−Ｌ−アスパルチル−Ｌ−フェニルアラニン−α−メチルエステルの製造方法
JP2005040037A (ja) * 2003-07-25 2005-02-17 Ajinomoto Co Inc ジペプチドの製造方法、それに用いるペプチド生成酵素、およびペプチド生成酵素の製造方法
US20050106703A1 (en) * 2003-11-05 2005-05-19 Kyowa Hakko Kogyo Co., Ltd. Microorganisms producing dipeptides and process for producing dipeptides using the microorganisms
US20080213827A1 (en) * 2004-06-25 2008-09-04 Hashimoto Shin-Ichi Process For Producing Dipeptides or Dipeptide Derivatives
DE602005017707D1 (de) * 2004-06-25 2009-12-31 Kyowa Hakko Bio Co Ltd Verfahren zur Herstellung von Dipeptiden oder Dipeptidderivaten.

Also Published As

Publication number Publication date
KR20010112931A (ko) 2001-12-22
EA200101015A1 (ru) 2002-02-28
AU3843900A (en) 2000-10-16
BR0010398A (pt) 2002-12-03
CN1353757A (zh) 2002-06-12
MXPA01009875A (es) 2002-04-24
EP1165799A1 (fr) 2002-01-02
JP2002539835A (ja) 2002-11-26

Similar Documents

Publication Publication Date Title
CN1973042B (zh) 二肽的制造方法
US20100285563A1 (en) Non-Ribosomal Peptide Synthetases
US5795738A (en) Engineered peptide synthetases and their use for the non-ribosomal production of peptides
JPWO2004011653A1 (ja) 新規ペプチド生成酵素遺伝子
JP6020706B2 (ja) ジペプチド合成酵素（バリアント）をコードするｄｎａ、エシェリヒア属に属する細菌、およびそれらを用いるジペププドの生産方法
EP1096011A1 (fr) Procédé microbiologique pour la préparation de l'alpha-L-aspartyl-L-phénylalanine
Shu et al. Amino acids activated by fengycin synthetase FenE
CA2365594A1 (fr) Methode de production microbiologique d'.alpha.-l-aspartyl-l-phenylalanine
US12281340B2 (en) Method of producing N-acyl-amino group-containing compound
US20020064834A1 (en) Microbiological production method for alpha-L-aspartyl-L-phenylalanine
JP2007181452A (ja) デキストラン生成酵素遺伝子、デキストラン生成酵素およびその製造方法、デキストランの製造方法
WO2004011652A1 (fr) Procede de production de tripeptide ou de peptide superieur
EP1197557A1 (fr) Gene codant pour une acylase des lipopeptides cycliques et expression dudit gene
AU783603B2 (en) Transformant producing secondary metabolite modified with functional group and novel biosynthesis genes
JP4516712B2 (ja) Ｌ−ピペコリン酸の生物学的な製造方法
JP2009534048A (ja) シクロジペプチド合成酵素及びシクロ（Ｌｅｕ−Ｌｅｕ）シクロジペプチドの合成のためのその使用
WO2004076477A1 (fr) Procede pour la production microbiologique de $g(a)-l-aspartyl-l-phenylalanine
EP1543126B1 (fr) Vitamine b6-phosphate phosphatase codant un gene et son utilisation
EP4031557A1 (fr) Procédés de production de d-tryptophane et de d-tryptophanes substitués
WO2000008170A1 (fr) Gene participant a la production d'acide homoglutamique, et utilisation associee
US6077699A (en) Polypeptides involved in the biosynthesis of streptogramins, nucleotide sequences coding for these polypeptides and their use
US20050227325A1 (en) Recombinant polynucleotide
JP2024157855A (ja) 変異型テトラプレニル－β－クルクメン環化酵素及びそれを用いたアンブレインの製造方法
Lukk Discovery of function in the enolase superfamily
JPWO2001002585A1 (ja) 環状リポペプチドアシラーゼをコードする遺伝子およびその発現

Legal Events

Date Code Title Description
FZDE Dead