CA2367723A1 - Procedes d'obtention de sequences d'acide nucleique pleine longueur a l'aide de e. coli topoisomerase iii et ses homologues - Google Patents
Procedes d'obtention de sequences d'acide nucleique pleine longueur a l'aide de e. coli topoisomerase iii et ses homologues Download PDFInfo
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- CA2367723A1 CA2367723A1 CA002367723A CA2367723A CA2367723A1 CA 2367723 A1 CA2367723 A1 CA 2367723A1 CA 002367723 A CA002367723 A CA 002367723A CA 2367723 A CA2367723 A CA 2367723A CA 2367723 A1 CA2367723 A1 CA 2367723A1
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- 101150066555 lacZ gene Proteins 0.000 description 1
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- 229940069446 magnesium acetate Drugs 0.000 description 1
- 235000011285 magnesium acetate Nutrition 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
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- DCWXELXMIBXGTH-UHFFFAOYSA-N phosphotyrosine Chemical compound OC(=O)C(N)CC1=CC=C(OP(O)(O)=O)C=C1 DCWXELXMIBXGTH-UHFFFAOYSA-N 0.000 description 1
- 230000008488 polyadenylation Effects 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000037452 priming Effects 0.000 description 1
- 210000001236 prokaryotic cell Anatomy 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000000527 sonication Methods 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 229960005322 streptomycin Drugs 0.000 description 1
- 229960002180 tetracycline Drugs 0.000 description 1
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- 235000019364 tetracycline Nutrition 0.000 description 1
- 150000003522 tetracyclines Chemical class 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- RYYWUUFWQRZTIU-UHFFFAOYSA-K thiophosphate Chemical compound [O-]P([O-])([O-])=S RYYWUUFWQRZTIU-UHFFFAOYSA-K 0.000 description 1
- 101150004556 traE gene Proteins 0.000 description 1
- 230000002103 transcriptional effect Effects 0.000 description 1
- 239000001226 triphosphate Substances 0.000 description 1
- 235000011178 triphosphate Nutrition 0.000 description 1
- UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 description 1
- 241000701447 unidentified baculovirus Species 0.000 description 1
- 241001529453 unidentified herpesvirus Species 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/90—Isomerases (5.)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4702—Regulators; Modulating activity
- C07K14/4705—Regulators; Modulating activity stimulating, promoting or activating activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
Abstract
L'invention porte sur un procédé d'obtention d'un ADNc codant pleine longueur. Ce procédé consiste à isoler un ARNm pleine longueur afin de synthétiser un premier brin d'ADNc, à fixer une étiquette non indigène sur l'ADNc et à utiliser le premier brin étiqueté pour synthétiser le second brin d'ADNc. L'ADNc final peut être ensuite amplifié et inséré dans un vecteur d'expression. L'invention porte également sur des ADNc codant pleine longueur préparés selon ce procédé, ainsi que sur des produits de recombinaison d'acide nucléique contenant les ADNc codant pleine longueur.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US12512699P | 1999-03-19 | 1999-03-19 | |
| US60/125,126 | 1999-03-19 | ||
| PCT/US2000/006560 WO2000056878A1 (fr) | 1999-03-19 | 2000-03-13 | Procedes d'obtention de sequences d'acide nucleique pleine longueur a l'aide de e. coli topoisomerase iii et ses homologues |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2367723A1 true CA2367723A1 (fr) | 2000-09-28 |
Family
ID=22418306
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002367723A Abandoned CA2367723A1 (fr) | 1999-03-19 | 2000-03-13 | Procedes d'obtention de sequences d'acide nucleique pleine longueur a l'aide de e. coli topoisomerase iii et ses homologues |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP1165768A1 (fr) |
| JP (1) | JP2002539784A (fr) |
| AU (1) | AU3397800A (fr) |
| CA (1) | CA2367723A1 (fr) |
| WO (1) | WO2000056878A1 (fr) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5766891A (en) | 1994-12-19 | 1998-06-16 | Sloan-Kettering Institute For Cancer Research | Method for molecular cloning and polynucleotide synthesis using vaccinia DNA topoisomerase |
| EP0920526B1 (fr) | 1997-06-12 | 2004-11-24 | Sloan-Kettering Institute For Cancer Research | Assemblage covalent de brins d'adn a des brins d'arn catalyse par une vaccinia topoisomerase |
| US6326175B1 (en) * | 1999-07-13 | 2001-12-04 | Incyte Pharmaceuticals, Inc. | Methods and compositions for producing full length cDNA libraries |
| WO2001062943A1 (fr) | 2000-02-25 | 2001-08-30 | Invitrogen Corporation | Techniques d'amplification induites par des lieurs de topoisomerase |
| ATE315086T1 (de) | 2000-08-21 | 2006-02-15 | Invitrogen Corp | Methoden und reagenzien für molekulares klonieren |
| US7198924B2 (en) | 2000-12-11 | 2007-04-03 | Invitrogen Corporation | Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites |
| JP4340779B2 (ja) | 2000-10-05 | 2009-10-07 | 独立行政法人理化学研究所 | 可変突出部位を含むオリゴヌクレオチドリンカー及び前記リンカーを用いたポリヌクレオチドライブラリーの調製方法 |
| US20060008817A1 (en) | 2000-12-08 | 2006-01-12 | Invitrogen Corporation | Methods and compositions for generating recombinant nucleic acid molecules |
| WO2002061034A2 (fr) | 2000-12-08 | 2002-08-08 | Invitrogen Corporation | Compositions et methodes permettant de produire rapidement des molecules d'acide nucleique recombinees |
| WO2005054438A2 (fr) | 2003-12-01 | 2005-06-16 | Invitrogen Corporation | Molecule d'acide nucleique contenant des sites de recombinaison et leurs procedes d'utilisation |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2005252C (fr) * | 1988-12-13 | 2001-07-17 | Nahum Sonenberg | Isolement de clones d'adn-c pleine longueur et de mrna chapeautes |
| JP3337748B2 (ja) * | 1992-09-25 | 2002-10-21 | 財団法人神奈川科学技術アカデミー | 完全長ｃＤＮＡの合成方法、その中間体の製造方法及び完全長ｃＤＮＡを含む組換えベクターの製造方法 |
| US5766891A (en) * | 1994-12-19 | 1998-06-16 | Sloan-Kettering Institute For Cancer Research | Method for molecular cloning and polynucleotide synthesis using vaccinia DNA topoisomerase |
| FR2733762B1 (fr) * | 1995-05-02 | 1997-08-01 | Genset Sa | Methode de couplage specifique de la coiffe de l'extremite 5' d'un fragment d'arnm et preparation d'arnm et d'adnc complet |
| US5962271A (en) * | 1996-01-03 | 1999-10-05 | Cloutech Laboratories, Inc. | Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end |
| JP3441899B2 (ja) * | 1996-11-01 | 2003-09-02 | 理化学研究所 | 完全長ｃＤＮＡライブラリーの作成方法 |
| EP0988313A4 (fr) * | 1997-06-04 | 2004-11-17 | Smithkline Beecham Corp | METHODES DE CLONAGE RAPIDE D'ADNc DE LONGUEUR TOTALE |
| EP0920526B1 (fr) * | 1997-06-12 | 2004-11-24 | Sloan-Kettering Institute For Cancer Research | Assemblage covalent de brins d'adn a des brins d'arn catalyse par une vaccinia topoisomerase |
-
2000
- 2000-03-13 CA CA002367723A patent/CA2367723A1/fr not_active Abandoned
- 2000-03-13 JP JP2000606737A patent/JP2002539784A/ja active Pending
- 2000-03-13 EP EP00912216A patent/EP1165768A1/fr not_active Withdrawn
- 2000-03-13 WO PCT/US2000/006560 patent/WO2000056878A1/fr not_active Ceased
- 2000-03-13 AU AU33978/00A patent/AU3397800A/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| EP1165768A1 (fr) | 2002-01-02 |
| JP2002539784A (ja) | 2002-11-26 |
| WO2000056878A1 (fr) | 2000-09-28 |
| AU3397800A (en) | 2000-10-09 |
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| Date | Code | Title | Description |
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| FZDE | Discontinued |