ATE315086T1 - Methoden und reagenzien für molekulares klonieren - Google Patents

Methoden und reagenzien für molekulares klonieren

Info

Publication number
ATE315086T1
ATE315086T1 AT01968079T AT01968079T ATE315086T1 AT E315086 T1 ATE315086 T1 AT E315086T1 AT 01968079 T AT01968079 T AT 01968079T AT 01968079 T AT01968079 T AT 01968079T AT E315086 T1 ATE315086 T1 AT E315086T1
Authority
AT
Austria
Prior art keywords
methods
nucleic acid
acid molecules
kits
compositions
Prior art date
Application number
AT01968079T
Other languages
English (en)
Inventor
Jonathan D Chesnut
Stewart Shuman
Knut R Madden
John Heyman
Robert P Bennett
Original Assignee
Invitrogen Corp
Sloan Kettering Inst Cancer
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Invitrogen Corp, Sloan Kettering Inst Cancer filed Critical Invitrogen Corp
Application granted granted Critical
Publication of ATE315086T1 publication Critical patent/ATE315086T1/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Polyesters Or Polycarbonates (AREA)
AT01968079T 2000-08-21 2001-08-21 Methoden und reagenzien für molekulares klonieren ATE315086T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US22656300P 2000-08-21 2000-08-21

Publications (1)

Publication Number Publication Date
ATE315086T1 true ATE315086T1 (de) 2006-02-15

Family

ID=22849413

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01968079T ATE315086T1 (de) 2000-08-21 2001-08-21 Methoden und reagenzien für molekulares klonieren

Country Status (9)

Country Link
US (6) US6916632B2 (de)
EP (1) EP1313851B1 (de)
JP (1) JP5043277B2 (de)
AT (1) ATE315086T1 (de)
AU (2) AU2001288356B2 (de)
CA (1) CA2421057A1 (de)
DE (1) DE60116506T9 (de)
NZ (1) NZ524135A (de)
WO (1) WO2002016594A2 (de)

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NZ539430A (en) 1999-12-10 2006-09-29 Invitrogen Corp Use of multiple recombination sites with unique specificity in recombinational cloning
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US7244560B2 (en) * 2000-05-21 2007-07-17 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US7198924B2 (en) * 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
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AU2003273995A1 (en) * 2002-06-05 2003-12-22 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
WO2005028615A2 (en) * 2003-06-26 2005-03-31 Invitrogen Corporation Methods and compositions for detecting promoter activity and expressing fusion proteins
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US10253321B2 (en) 2013-05-01 2019-04-09 Dna2.0, Inc. Methods, compositions and kits for a one-step DNA cloning system
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CN103642829B (zh) * 2013-12-13 2016-05-18 北京全式金生物技术有限公司 一种高效率基因定向克隆的方法
WO2016011174A1 (en) 2014-07-15 2016-01-21 Life Technologies Corporation Compositions and methods for nucleic acid assembly
FR3028527A1 (fr) 2014-11-13 2016-05-20 Pivert Identification de facteurs de transcription de yarrowia lipolytica
JP2020530979A (ja) 2017-06-30 2020-11-05 インスクリプタ， インコーポレイテッド 自動細胞処理方法、モジュール、機器及びシステム
US10738327B2 (en) 2017-08-28 2020-08-11 Inscripta, Inc. Electroporation cuvettes for automation
JP6985508B2 (ja) 2017-09-30 2021-12-22 インスクリプタ， インコーポレイテッド フロースルーエレクトロポレーション機器編成
US10443031B1 (en) 2018-03-29 2019-10-15 Inscripta, Inc. Methods for controlling the growth of prokaryotic and eukaryotic cells
WO2019200004A1 (en) 2018-04-13 2019-10-17 Inscripta, Inc. Automated cell processing instruments comprising reagent cartridges
WO2019209926A1 (en) 2018-04-24 2019-10-31 Inscripta, Inc. Automated instrumentation for production of peptide libraries
US10557216B2 (en) 2018-04-24 2020-02-11 Inscripta, Inc. Automated instrumentation for production of T-cell receptor peptide libraries
US10858761B2 (en) 2018-04-24 2020-12-08 Inscripta, Inc. Nucleic acid-guided editing of exogenous polynucleotides in heterologous cells
CA3108767A1 (en) 2018-06-30 2020-01-02 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
US11142740B2 (en) 2018-08-14 2021-10-12 Inscripta, Inc. Detection of nuclease edited sequences in automated modules and instruments
US10532324B1 (en) 2018-08-14 2020-01-14 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
WO2020081149A2 (en) 2018-08-30 2020-04-23 Inscripta, Inc. Improved detection of nuclease edited sequences in automated modules and instruments
WO2020185967A1 (en) 2019-03-11 2020-09-17 Red Genomics, Inc. Methods and reagents for enhanced next generation sequencing library conversion and insertion of barcodes into nucleic acids.
WO2020257395A1 (en) 2019-06-21 2020-12-24 Inscripta, Inc. Genome-wide rationally-designed mutations leading to enhanced lysine production in e. coli
US10927385B2 (en) 2019-06-25 2021-02-23 Inscripta, Inc. Increased nucleic-acid guided cell editing in yeast
US10689669B1 (en) 2020-01-11 2020-06-23 Inscripta, Inc. Automated multi-module cell processing methods, instruments, and systems
US20210332388A1 (en) 2020-04-24 2021-10-28 Inscripta, Inc. Compositions, methods, modules and instruments for automated nucleic acid-guided nuclease editing in mammalian cells
US11787841B2 (en) 2020-05-19 2023-10-17 Inscripta, Inc. Rationally-designed mutations to the thrA gene for enhanced lysine production in E. coli
WO2023200770A1 (en) 2022-04-12 2023-10-19 Inscripta, Inc. Curing for iterative nucleic acid-guided nuclease editing
WO2024238666A1 (en) 2023-05-16 2024-11-21 Inscripta, Inc. Methods to enable insertion of large fragments of dna into genomes of live cells at library scale

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Also Published As

Publication number Publication date
US8883991B2 (en) 2014-11-11
US20050282184A1 (en) 2005-12-22
AU8835601A (en) 2002-03-04
DE60116506D1 (de) 2006-03-30
US20090328244A1 (en) 2009-12-31
DE60116506T9 (de) 2007-02-22
NZ524135A (en) 2004-08-27
US20150093788A1 (en) 2015-04-02
EP1313851B1 (de) 2006-01-04
JP2004517608A (ja) 2004-06-17
WO2002016594A3 (en) 2003-02-13
JP5043277B2 (ja) 2012-10-10
EP1313851A2 (de) 2003-05-28
US6916632B2 (en) 2005-07-12
US7528241B2 (en) 2009-05-05
WO2002016594A2 (en) 2002-02-28
US20120129225A1 (en) 2012-05-24
AU2001288356B2 (en) 2007-03-22
DE60116506T2 (de) 2006-09-28
US20130323797A1 (en) 2013-12-05
US20030022179A1 (en) 2003-01-30
CA2421057A1 (en) 2002-02-28

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