CA2369002A1 - Procedes de purification d'adn a l'aide de sondes de capture immobilisees - Google Patents

Procedes de purification d'adn a l'aide de sondes de capture immobilisees Download PDF

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Publication number
CA2369002A1
CA2369002A1 CA002369002A CA2369002A CA2369002A1 CA 2369002 A1 CA2369002 A1 CA 2369002A1 CA 002369002 A CA002369002 A CA 002369002A CA 2369002 A CA2369002 A CA 2369002A CA 2369002 A1 CA2369002 A1 CA 2369002A1
Authority
CA
Canada
Prior art keywords
sequencing reaction
capture probe
primer extension
gel
electrophoretic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002369002A
Other languages
English (en)
Inventor
Christopher P. Adams
T. Christian Boles
Lawrence Weir
Rahul K. Dhanda
Nevin M. Summers
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Exact Sciences Corp
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2369002A1 publication Critical patent/CA2369002A1/fr
Abandoned legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/405—Concentrating samples by adsorption or absorption
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N2035/1027—General features of the devices
    • G01N2035/1048—General features of the devices using the transfer device for another function
    • G01N2035/1053—General features of the devices using the transfer device for another function for separating part of the liquid, e.g. filters, extraction phase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention concerne des procédés utilisant des dispositifs de purification comprenant un milieu électrophorétique contenant des sondes de capture immobilisées pour la purification de l'ADN.
CA002369002A 1999-02-26 2000-02-25 Procedes de purification d'adn a l'aide de sondes de capture immobilisees Abandoned CA2369002A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US25946799A 1999-02-26 1999-02-26
US09/259,467 1999-02-26
PCT/US2000/004939 WO2000050644A2 (fr) 1999-02-26 2000-02-25 Procedes de purification d'adn a l'aide de sondes de capture immobilisees

Publications (1)

Publication Number Publication Date
CA2369002A1 true CA2369002A1 (fr) 2000-08-31

Family

ID=22985081

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002369002A Abandoned CA2369002A1 (fr) 1999-02-26 2000-02-25 Procedes de purification d'adn a l'aide de sondes de capture immobilisees

Country Status (5)

Country Link
EP (1) EP1163371A2 (fr)
JP (1) JP2002537778A (fr)
AU (1) AU3708300A (fr)
CA (1) CA2369002A1 (fr)
WO (1) WO2000050644A2 (fr)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE335845T1 (de) 1997-05-16 2006-09-15 Exact Sciences Corp Elektrophoretische analyse von molekülen mit immobilisierten sonden
WO1999022228A1 (fr) * 1997-10-24 1999-05-06 Northeastern University Microsysteme multicanaux de separation preparative a haut rendement a collecte et analyse exhaustive
JP4117249B2 (ja) 2001-10-15 2008-07-16 バイオセプト インコーポレイテッド マイクロウェルバイオチップ
JP2005110503A (ja) * 2003-10-02 2005-04-28 Arkray Inc 核酸の精製方法及びデバイス
WO2005047881A2 (fr) 2003-11-05 2005-05-26 Exact Sciences Corporation Techniques de separation repetitive par affinite et leurs utilisations

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5639603A (en) * 1991-09-18 1997-06-17 Affymax Technologies N.V. Synthesizing and screening molecular diversity
CA2105962A1 (fr) * 1992-09-18 1994-03-19 Margaret Patricia Raybuck Dispositif et procede de separation d'affinite
US5876918A (en) * 1993-03-08 1999-03-02 Hydros, Inc. Aligned fiber diagnostic chromatography with positive and negative controls
US5560811A (en) * 1995-03-21 1996-10-01 Seurat Analytical Systems Incorporated Capillary electrophoresis apparatus and method

Also Published As

Publication number Publication date
WO2000050644A2 (fr) 2000-08-31
AU3708300A (en) 2000-09-14
WO2000050644A3 (fr) 2001-04-05
JP2002537778A (ja) 2002-11-12
EP1163371A2 (fr) 2001-12-19

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Dead