CA2384756C - Reversed-phase hplc assay for plasminogen activators - Google Patents

Reversed-phase hplc assay for plasminogen activators Download PDF

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Publication number
CA2384756C
CA2384756C CA2384756A CA2384756A CA2384756C CA 2384756 C CA2384756 C CA 2384756C CA 2384756 A CA2384756 A CA 2384756A CA 2384756 A CA2384756 A CA 2384756A CA 2384756 C CA2384756 C CA 2384756C
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CA
Canada
Prior art keywords
tpa
cho
sample
tnk
endogenous
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA2384756A
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English (en)
French (fr)
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CA2384756A1 (en
Inventor
Yuan Xu
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Genentech Inc
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Genentech Inc
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Publication of CA2384756A1 publication Critical patent/CA2384756A1/en
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Publication of CA2384756C publication Critical patent/CA2384756C/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/972—Plasminogen activators
    • G01N2333/9726—Tissue plasminogen activator
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Devices For Conveying Motion By Means Of Endless Flexible Members (AREA)
  • Mechanical Treatment Of Semiconductor (AREA)
  • Measuring And Recording Apparatus For Diagnosis (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Measuring Pulse, Heart Rate, Blood Pressure Or Blood Flow (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
  • Signal Processing For Digital Recording And Reproducing (AREA)
  • Glass Compositions (AREA)
CA2384756A 1999-11-04 2000-11-01 Reversed-phase hplc assay for plasminogen activators Expired - Lifetime CA2384756C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US16360799P 1999-11-04 1999-11-04
US60/163,607 1999-11-04
PCT/US2000/030252 WO2001032915A2 (en) 1999-11-04 2000-11-01 Reversed-phase hplc assay for plasminogen activators

Publications (2)

Publication Number Publication Date
CA2384756A1 CA2384756A1 (en) 2001-05-10
CA2384756C true CA2384756C (en) 2010-09-28

Family

ID=22590762

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2384756A Expired - Lifetime CA2384756C (en) 1999-11-04 2000-11-01 Reversed-phase hplc assay for plasminogen activators

Country Status (24)

Country Link
US (2) US6593097B1 (cs)
EP (1) EP1226269B1 (cs)
JP (1) JP4659320B2 (cs)
KR (1) KR100649043B1 (cs)
CN (1) CN1167808C (cs)
AT (1) ATE243263T1 (cs)
AU (1) AU781023B2 (cs)
BR (1) BRPI0015283B8 (cs)
CA (1) CA2384756C (cs)
CZ (1) CZ302023B6 (cs)
DE (1) DE60003449T2 (cs)
DK (1) DK1226269T3 (cs)
ES (1) ES2200973T3 (cs)
HK (1) HK1046158B (cs)
HU (1) HU226202B1 (cs)
IL (1) IL149006A0 (cs)
MX (1) MXPA02004401A (cs)
NO (1) NO329876B1 (cs)
NZ (1) NZ518187A (cs)
PL (1) PL204011B1 (cs)
PT (1) PT1226269E (cs)
SI (1) SI1226269T1 (cs)
WO (1) WO2001032915A2 (cs)
ZA (1) ZA200202687B (cs)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE391789T1 (de) 1999-12-10 2008-04-15 Invitrogen Corp Verwendung einer vielzahl von rekombinationsstellen mit einzigartiger spezifität beim rekombinatorischen klonen
EP2105496B1 (en) 2000-12-08 2013-02-20 Life Technologies Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US6972327B1 (en) * 2001-05-08 2005-12-06 Immunex Corporation Regeneration of chromatography material
WO2006126942A1 (en) * 2005-05-24 2006-11-30 Ge Healthcare Bio-Sciences Ab Regeneration of a chromatography matrix
KR100701265B1 (ko) * 2006-01-16 2007-04-02 대한민국 재조합 사람 조직형 플라스미노겐 활성인자 발현벡터 및 플라스미노겐 활성인자를 소변으로 분비하는 형질전환돼지 생산방법
TW200821026A (en) * 2006-09-08 2008-05-16 Wyeth Corp Arginine wash in protein purification using affinity chromatography
EP2407783A1 (en) * 2006-10-27 2012-01-18 Janssen Pharmaceutica, N.V. A method for pharmacologically profiling compounds
CN101539550B (zh) * 2009-04-28 2012-05-23 李绍平 用于多糖的定性与定量分析方法
EP3592858B1 (en) 2017-03-09 2024-06-12 Universität des Saarlandes Means and methods for lignin pyrolysis
CN108333281A (zh) * 2018-02-08 2018-07-27 吉林大学 一种测定重组人粒细胞集落刺激因子二硫键组成的方法
CN108918749B (zh) * 2018-07-17 2020-05-05 北京赛升药业股份有限公司 一种蛇毒纤溶酶的反相hplc检测方法
CN114426962B (zh) * 2021-12-21 2024-06-18 佛山汉腾生物科技有限公司 t-PA纯化方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4766075A (en) 1982-07-14 1988-08-23 Genentech, Inc. Human tissue plasminogen activator
FI831484A7 (fi) 1982-05-05 1983-11-06 Genentech Inc Ihmiskudoksen plasminogeenien aktivaattori.
US4777043A (en) * 1985-12-17 1988-10-11 Genentech, Inc. Stabilized human tissue plasminogen activator compositions
US5120535A (en) * 1986-11-26 1992-06-09 Oncogen Oncostatin M and novel compositions having anti-neoplastic activity
US5411864A (en) * 1987-11-05 1995-05-02 Genentech, Inc. Method of purifying recombinant proteins from corresponding host cell proteins
GB8919803D0 (en) * 1989-09-01 1989-10-18 Ciba Geigy Pharmaceutical compositions
JP3559559B2 (ja) 1992-06-03 2004-09-02 ジェネンテク，インコーポレイテッド 向上した治療特性を有する組織プラスミノーゲン活性化因子グリコシル化変異体
US5753702A (en) * 1996-05-22 1998-05-19 University Of Vermont Arachidonic acid metabolite, 16-hete

Also Published As

Publication number Publication date
PL204011B1 (pl) 2009-12-31
EP1226269A2 (en) 2002-07-31
CZ20021196A3 (cs) 2002-08-14
KR20020070431A (ko) 2002-09-09
CN1387579A (zh) 2002-12-25
BRPI0015283B1 (pt) 2015-03-17
MXPA02004401A (es) 2002-09-02
HU226202B1 (en) 2008-06-30
ATE243263T1 (de) 2003-07-15
DE60003449T2 (de) 2004-05-06
HK1046158A1 (en) 2002-12-27
NO20022122D0 (no) 2002-05-03
JP4659320B2 (ja) 2011-03-30
DK1226269T3 (da) 2003-10-13
WO2001032915A2 (en) 2001-05-10
BRPI0015283A (pt) 2002-07-02
NO329876B1 (no) 2011-01-17
WO2001032915A3 (en) 2002-03-07
DE60003449D1 (de) 2003-07-24
BRPI0015283B8 (pt) 2021-07-06
NO20022122L (no) 2002-07-03
KR100649043B1 (ko) 2006-11-27
HUP0202973A3 (en) 2005-11-28
PL355389A1 (en) 2004-04-19
HK1046158B (en) 2003-11-28
US6828118B2 (en) 2004-12-07
HUP0202973A2 (hu) 2002-12-28
CZ302023B6 (cs) 2010-09-08
PT1226269E (pt) 2003-11-28
US6593097B1 (en) 2003-07-15
AU1581601A (en) 2001-05-14
ZA200202687B (en) 2003-06-25
ES2200973T3 (es) 2004-03-16
JP2003513638A (ja) 2003-04-15
IL149006A0 (en) 2002-11-10
US20030199016A1 (en) 2003-10-23
NZ518187A (en) 2002-12-20
EP1226269B1 (en) 2003-06-18
CA2384756A1 (en) 2001-05-10
CN1167808C (zh) 2004-09-22
SI1226269T1 (en) 2003-12-31
AU781023B2 (en) 2005-04-28

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