CA2393703A1 - Systeme d'essai permettant de detecter des analytes, un procede de realisation, et son utilisation - Google Patents
Systeme d'essai permettant de detecter des analytes, un procede de realisation, et son utilisation Download PDFInfo
- Publication number
- CA2393703A1 CA2393703A1 CA002393703A CA2393703A CA2393703A1 CA 2393703 A1 CA2393703 A1 CA 2393703A1 CA 002393703 A CA002393703 A CA 002393703A CA 2393703 A CA2393703 A CA 2393703A CA 2393703 A1 CA2393703 A1 CA 2393703A1
- Authority
- CA
- Canada
- Prior art keywords
- assay system
- pcr
- analyte
- polypeptide
- rna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 238000003556 assay Methods 0.000 title claims description 19
- 238000000034 method Methods 0.000 title claims description 16
- 238000002360 preparation method Methods 0.000 title description 2
- 238000001514 detection method Methods 0.000 claims abstract description 13
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 11
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 11
- 150000007523 nucleic acids Chemical class 0.000 claims abstract description 11
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 11
- 102000004196 processed proteins & peptides Human genes 0.000 claims abstract description 9
- 229920001184 polypeptide Polymers 0.000 claims abstract description 7
- 239000012491 analyte Substances 0.000 claims description 15
- RXWNCPJZOCPEPQ-NVWDDTSBSA-N puromycin Chemical group C1=CC(OC)=CC=C1C[C@H](N)C(=O)N[C@H]1[C@@H](O)[C@H](N2C3=NC=NC(=C3N=C2)N(C)C)O[C@@H]1CO RXWNCPJZOCPEPQ-NVWDDTSBSA-N 0.000 claims description 9
- 230000027455 binding Effects 0.000 claims description 8
- 238000005406 washing Methods 0.000 claims description 7
- 230000003321 amplification Effects 0.000 claims description 6
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 6
- 239000000427 antigen Substances 0.000 claims description 4
- 102000036639 antigens Human genes 0.000 claims description 4
- 108091007433 antigens Proteins 0.000 claims description 4
- 229950010131 puromycin Drugs 0.000 claims description 4
- 229920001222 biopolymer Polymers 0.000 claims description 3
- 150000002894 organic compounds Chemical class 0.000 claims description 3
- 102000004127 Cytokines Human genes 0.000 claims description 2
- 108090000695 Cytokines Proteins 0.000 claims description 2
- 238000006073 displacement reaction Methods 0.000 claims description 2
- 239000003814 drug Substances 0.000 claims description 2
- 239000004009 herbicide Substances 0.000 claims description 2
- 239000005556 hormone Substances 0.000 claims description 2
- 229940088597 hormone Drugs 0.000 claims description 2
- 229940127240 opiate Drugs 0.000 claims description 2
- 239000000575 pesticide Substances 0.000 claims description 2
- 239000003016 pheromone Substances 0.000 claims description 2
- 229930000044 secondary metabolite Natural products 0.000 claims description 2
- 238000000099 in vitro assay Methods 0.000 abstract description 2
- 102000004169 proteins and genes Human genes 0.000 description 13
- 108090000623 proteins and genes Proteins 0.000 description 13
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 11
- 108020004414 DNA Proteins 0.000 description 11
- 239000000562 conjugate Substances 0.000 description 9
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 8
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 8
- 239000000126 substance Substances 0.000 description 8
- 238000006243 chemical reaction Methods 0.000 description 7
- 108010090804 Streptavidin Proteins 0.000 description 5
- 238000013519 translation Methods 0.000 description 5
- 229960002685 biotin Drugs 0.000 description 4
- 235000020958 biotin Nutrition 0.000 description 4
- 239000011616 biotin Substances 0.000 description 4
- 230000001900 immune effect Effects 0.000 description 4
- 229910001629 magnesium chloride Inorganic materials 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 230000035945 sensitivity Effects 0.000 description 4
- 108090001008 Avidin Proteins 0.000 description 3
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 3
- 101710135898 Myc proto-oncogene protein Proteins 0.000 description 3
- 102100038895 Myc proto-oncogene protein Human genes 0.000 description 3
- 238000012408 PCR amplification Methods 0.000 description 3
- 101710150448 Transcriptional regulator Myc Proteins 0.000 description 3
- 239000013504 Triton X-100 Substances 0.000 description 3
- 229920004890 Triton X-100 Polymers 0.000 description 3
- 239000001913 cellulose Substances 0.000 description 3
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- 238000011534 incubation Methods 0.000 description 3
- 238000002372 labelling Methods 0.000 description 3
- 239000003550 marker Substances 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 2
- 108010006785 Taq Polymerase Proteins 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 230000008878 coupling Effects 0.000 description 2
- 238000010168 coupling process Methods 0.000 description 2
- 238000005859 coupling reaction Methods 0.000 description 2
- 229910000397 disodium phosphate Inorganic materials 0.000 description 2
- 230000004927 fusion Effects 0.000 description 2
- 239000000499 gel Substances 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- 230000008105 immune reaction Effects 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 239000000523 sample Substances 0.000 description 2
- 229910000162 sodium phosphate Inorganic materials 0.000 description 2
- 241000972773 Aulopiformes Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 108091026890 Coding region Proteins 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 1
- 102100034343 Integrase Human genes 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 108020004711 Nucleic Acid Probes Proteins 0.000 description 1
- 108091034117 Oligonucleotide Proteins 0.000 description 1
- 229920001213 Polysorbate 20 Polymers 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 1
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 1
- 229920005654 Sephadex Polymers 0.000 description 1
- 239000012507 Sephadex™ Substances 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- 101710172711 Structural protein Proteins 0.000 description 1
- 241000723873 Tobacco mosaic virus Species 0.000 description 1
- 108020004566 Transfer RNA Proteins 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 125000003275 alpha amino acid group Chemical group 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 239000011230 binding agent Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 239000000919 ceramic Substances 0.000 description 1
- 239000002299 complementary DNA Substances 0.000 description 1
- 239000002178 crystalline material Substances 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 238000001215 fluorescent labelling Methods 0.000 description 1
- 238000005194 fractionation Methods 0.000 description 1
- 102000037865 fusion proteins Human genes 0.000 description 1
- 108020001507 fusion proteins Proteins 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 230000003100 immobilizing effect Effects 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 238000004020 luminiscence type Methods 0.000 description 1
- 239000006166 lysate Substances 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- MYWUZJCMWCOHBA-VIFPVBQESA-N methamphetamine Chemical compound CN[C@@H](C)CC1=CC=CC=C1 MYWUZJCMWCOHBA-VIFPVBQESA-N 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 108091027963 non-coding RNA Proteins 0.000 description 1
- 102000042567 non-coding RNA Human genes 0.000 description 1
- 239000002853 nucleic acid probe Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000000863 peptide conjugate Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000012340 reverse transcriptase PCR Methods 0.000 description 1
- 238000010839 reverse transcription Methods 0.000 description 1
- 235000019515 salmon Nutrition 0.000 description 1
- 238000010187 selection method Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 235000020183 skimmed milk Nutrition 0.000 description 1
- 238000010532 solid phase synthesis reaction Methods 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 230000009870 specific binding Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 230000014621 translational initiation Effects 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2458/00—Labels used in chemical analysis of biological material
- G01N2458/10—Oligonucleotides as tagging agents for labelling antibodies
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
La présente invention concerne un essai in vitro permettant la détection directe sensible et spécifique d'analytes. Pour cela, on utilise avantageusement des conjugués polypetide-acide nucléique qui servent de système de détection-amplification et dont les signaux sont amplifiés par PCR.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19959857.6 | 1999-12-10 | ||
| DE19959857A DE19959857C1 (de) | 1999-12-10 | 1999-12-10 | Testsystem zum Nachweis von Analyten sowie ein Verfahren zur Herstellung und Verwendung |
| PCT/EP2000/010336 WO2001042494A2 (fr) | 1999-12-10 | 2000-10-20 | Systeme d'essai permettant de detecter des analytes, un procede de realisation, et son utilisation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2393703A1 true CA2393703A1 (fr) | 2001-06-14 |
Family
ID=7932340
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002393703A Abandoned CA2393703A1 (fr) | 1999-12-10 | 2000-10-20 | Systeme d'essai permettant de detecter des analytes, un procede de realisation, et son utilisation |
Country Status (6)
| Country | Link |
|---|---|
| EP (1) | EP1240356A2 (fr) |
| JP (1) | JP2003528298A (fr) |
| AU (1) | AU1385901A (fr) |
| CA (1) | CA2393703A1 (fr) |
| DE (1) | DE19959857C1 (fr) |
| WO (1) | WO2001042494A2 (fr) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7842456B2 (en) | 2004-02-20 | 2010-11-30 | The Trustees Of The University Of Pennsylvania | Reagents, kits and methods for immunodetection of epitopes on molecules |
| US20210381026A1 (en) * | 2019-02-22 | 2021-12-09 | Epsilon Molecular Engineering Inc. | NOVEL IMMUNO-PCR METHOD USING cDNA DISPLAY |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB201218909D0 (en) * | 2012-10-22 | 2012-12-05 | Univ Singapore | Assay for the parallel detection of biological material based on PCR |
| JP2024521001A (ja) * | 2021-05-25 | 2024-05-28 | カビディ エービー | 高感度な分析物検出アッセイのための方法及びそのためのキット |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6083689A (en) * | 1990-10-16 | 2000-07-04 | Bayer Corporation | Sensitive immunoassays utilizing antibody conjugates with replicable DNA templates |
| US5665539A (en) * | 1991-07-12 | 1997-09-09 | The Regents Of The University Of California | Immuno-polymerase chain reaction system for antigen detection |
| US5922553A (en) * | 1996-11-21 | 1999-07-13 | Trustees Of The University Of Pennsylvania | Method of detecting protein by immuno RNA |
| DK1712623T3 (da) * | 1997-01-21 | 2012-02-06 | Gen Hospital Corp | Udvælgelse af proteiner ved anvendelse af RNA-proteinfusioner |
| AU3463699A (en) * | 1998-04-03 | 1999-10-25 | Phylos, Inc. | Addressable protein arrays |
| AU779653B2 (en) * | 1999-08-27 | 2005-02-03 | Bristol-Myers Squibb Company | Methods for encoding and sorting in vitro translated proteins |
-
1999
- 1999-12-10 DE DE19959857A patent/DE19959857C1/de not_active Revoked
-
2000
- 2000-10-20 AU AU13859/01A patent/AU1385901A/en not_active Abandoned
- 2000-10-20 JP JP2001544366A patent/JP2003528298A/ja active Pending
- 2000-10-20 CA CA002393703A patent/CA2393703A1/fr not_active Abandoned
- 2000-10-20 WO PCT/EP2000/010336 patent/WO2001042494A2/fr not_active Ceased
- 2000-10-20 EP EP00975889A patent/EP1240356A2/fr not_active Withdrawn
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7842456B2 (en) | 2004-02-20 | 2010-11-30 | The Trustees Of The University Of Pennsylvania | Reagents, kits and methods for immunodetection of epitopes on molecules |
| US20210381026A1 (en) * | 2019-02-22 | 2021-12-09 | Epsilon Molecular Engineering Inc. | NOVEL IMMUNO-PCR METHOD USING cDNA DISPLAY |
Also Published As
| Publication number | Publication date |
|---|---|
| AU1385901A (en) | 2001-06-18 |
| WO2001042494A3 (fr) | 2002-05-02 |
| WO2001042494A2 (fr) | 2001-06-14 |
| DE19959857C1 (de) | 2001-06-28 |
| JP2003528298A (ja) | 2003-09-24 |
| EP1240356A2 (fr) | 2002-09-18 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FZDE | Dead |