CA2408616C - Evaluation biologique, analyse et manipulation d'ovocytes et/ou d'embryons dans un canal microfluidique - Google Patents

Evaluation biologique, analyse et manipulation d'ovocytes et/ou d'embryons dans un canal microfluidique Download PDF

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Publication number
CA2408616C
CA2408616C CA002408616A CA2408616A CA2408616C CA 2408616 C CA2408616 C CA 2408616C CA 002408616 A CA002408616 A CA 002408616A CA 2408616 A CA2408616 A CA 2408616A CA 2408616 C CA2408616 C CA 2408616C
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Prior art keywords
embryo
fluid
embryos
channel
fluid channel
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Expired - Fee Related
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CA002408616A
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CA2408616A1 (fr
Inventor
David J. Beebe
Ian K. Glasgow
Matthew B. Wheeler
Henry Zeringue
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University of Illinois System
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University of Illinois System
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502776—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for focusing or laminating flows
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502746—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means for controlling flow resistance, e.g. flow controllers, baffles or throttle valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/06—Bioreactors or fermenters specially adapted for specific uses for in vitro fertilization
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0636—Focussing flows, e.g. to laminate flows
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0418—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic electro-osmotic flow [EOF]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/08—Regulating or influencing the flow resistance
    • B01L2400/084—Passive control of flow resistance
    • B01L2400/086—Passive control of flow resistance using baffles or other fixed flow obstructions

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Genetics & Genomics (AREA)
  • Dispersion Chemistry (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Sustainable Development (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Fluid Mechanics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

L'invention concerne des canaux (14) microfluidiques à l'échelle embryonnaire conçus pour manipuler et mettre en place des embryons, permettant de mieux évaluer et traiter des embryons. Un écoulement fluidique est utilisé pour acheminer et mettre en place les embryons à l'intérieur de canaux microfluidiques; des géométries de canaux pouvant être utilisées pour placer des embryons dans des emplacements spécifiques. Les propriétés de surface et les propriétés de compliance (déformation) des embryons sont évaluées pour servir d'indicateur prévisionnel de la viabilité. Les canaux microfluidiques permettent les réglages précis de la pression de manière à diriger diverses évaluations à des forces sensiblement inférieures à celles qui pourraient endommager les embryons. Le procédé décrit dans l'invention permet également d'évaluer la distance et/ou les embryons qui circulent dans un canal microfluidique à un gradient de pression identique et qui fournissent des informations, les embryons sains se déplaçant plus lentement ou sur une distance plus courte étant donné qu'ils présentent un frottement plus important contre les parois du canal. Placés à une constriction (14a, 14b, 24, 26), les embryons sains semblent, en outre, se déformer moins que les embryons malades qui sont plus rapidement attirés dans une constriction. De plus, les embryons sains semblent reprendre plus facilement leur forme. Le fluide provenant des canaux microfluidiques est facilement récupéré en aval sans endommager l'environnement embryonnaire, ce qui permet une meilleure analyse chimique dudit fluide en comparaison avec les techniques de manipulation et d'échantillonnage manuelles classiques. Le retrait de la zone pellucide des embryons mammifères est effectué au fur et à mesure que les embryons sont acheminés à travers l'écoulement vers un emplacement précis; un agent lytique pouvant être retiré de la surface de l'embryon afin de compléter le retrait de la zone pellucide. Le retrait du cumulus est effectué à l'aide d'une série de constrictions permettant de couper le cumulus, puis des écoulements fluidiques sont appliqués de manière à retirer le cumulus coupé de l'embryon.
CA002408616A 2000-05-12 2001-05-08 Evaluation biologique, analyse et manipulation d'ovocytes et/ou d'embryons dans un canal microfluidique Expired - Fee Related CA2408616C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/570,483 US6695765B1 (en) 1999-04-08 2000-05-12 Microfluidic channel embryo and/or oocyte handling, analysis and biological evaluation
US09/570,483 2000-05-12
PCT/US2001/014822 WO2001088087A2 (fr) 2000-05-12 2001-05-08 Evaluation biologique, analyse et manipulation d'ovocytes et/ou d'embryons dans un canal microfluidique

Publications (2)

Publication Number Publication Date
CA2408616A1 CA2408616A1 (fr) 2001-11-22
CA2408616C true CA2408616C (fr) 2007-10-02

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CA002408616A Expired - Fee Related CA2408616C (fr) 2000-05-12 2001-05-08 Evaluation biologique, analyse et manipulation d'ovocytes et/ou d'embryons dans un canal microfluidique

Country Status (11)

Country Link
EP (1) EP1280465A4 (fr)
JP (1) JP2003533221A (fr)
KR (1) KR20030009482A (fr)
CN (1) CN1441652A (fr)
AU (2) AU5963501A (fr)
BR (1) BR0110768A (fr)
CA (1) CA2408616C (fr)
IL (1) IL152650A (fr)
MX (1) MXPA02011150A (fr)
RU (1) RU2002130577A (fr)
WO (1) WO2001088087A2 (fr)

Families Citing this family (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2480728A1 (fr) * 2002-04-01 2003-10-16 Fluidigm Corporation Systemes d'analyse de particules microfluidiques
EP1636332A4 (fr) * 2003-05-06 2007-08-29 Bellbrook Labs Llc Cultures cellulaires tridimensionnelles dans un systeme de manipulation fluidique a petite echelle
CA2527733A1 (fr) * 2003-06-06 2004-12-16 The Regents Of The University Of Michigan Dispositif microfluidique integre d'isolation de sperme et d'insemination
GB0321158D0 (en) * 2003-09-10 2003-10-08 Central Research Lab Ltd Apparatus and method for handling cells,embryos or oocytes
EP1609850A1 (fr) 2004-06-24 2005-12-28 Biovir v/Jacob Mollenbach Boíte de culture pour cultiver des cellules biologiques
JP2006055072A (ja) * 2004-08-20 2006-03-02 Fujitsu Ltd 搬送制御装置及び方法
CA2601318A1 (fr) * 2005-03-18 2006-09-28 Eci, Inc. Instrument facilitant l'observation de cellules et procede d'observation de cellules l'utilisant
GB0508983D0 (en) 2005-05-03 2005-06-08 Oxford Gene Tech Ip Ltd Cell analyser
US20070084706A1 (en) * 2005-10-18 2007-04-19 Shuichi Takayama Microfluidic cell culture device and method for using same
US20070090166A1 (en) * 2005-10-18 2007-04-26 Shuichi Takayama Microfluidic cell culture device
US20110104730A1 (en) * 2007-08-24 2011-05-05 Smart Biosystems Aps Mesoscale bioreactor platform for perfusion
TW200920841A (en) * 2007-09-25 2009-05-16 Cytyc Corp Microfluidic apparatus for manipulating imaging and analyzing cells of a cytological specimen
KR100891487B1 (ko) * 2007-11-01 2009-04-01 한국과학기술원 미세유체제어기술 기반으로 한 난모세포 또는 난자의 핵제거 시스템
WO2009079474A1 (fr) * 2007-12-14 2009-06-25 The University Of North Carolina At Chapel Hill Procédés, systèmes et supports lisibles par ordinateur pour faciliter l'automatisation de la micro-injection de blastocystes
WO2009118014A1 (fr) * 2008-08-01 2009-10-01 Smart Biosystems Aps Chambre d'une plate-forme de bioréacteur
GB2464300A (en) * 2008-10-10 2010-04-14 Univ Dublin City Microfluidic multiplexed cellular and molecular analysis device and method
GB0821636D0 (en) 2008-11-26 2008-12-31 Ucl Business Plc Device
WO2010141320A1 (fr) * 2009-06-01 2010-12-09 Mariposa Biotechnology, Inc. Dispositif pour l'elimination de cumulus a partir d'ovocytes
US8621943B2 (en) * 2009-09-30 2014-01-07 Weyerhaeuser Nr Company Method of singulating embryos
EP2316565A1 (fr) * 2009-10-26 2011-05-04 Fei Company Microréacteur pour observer des particules dans un fluide
CN101947124B (zh) * 2010-06-25 2012-07-04 博奥生物有限公司 一种集成式微流控芯片装置及其使用方法
CA2812776C (fr) * 2010-09-27 2022-05-17 Auxogyn, Inc. Appareil, procede et systeme pour l'imagerie automatisee et l'evaluation d'embryons, d'ovocytes et de cellules souches
WO2013158658A1 (fr) * 2012-04-16 2013-10-24 Cornell University Système de fertilisation assistée par injection intracytoplasmique automatisée de sperme
AU2013202632B2 (en) * 2013-03-14 2015-04-16 Inguran, Llc Apparatus and methods for high throughput sperm sorting
AU2015203738B2 (en) * 2013-03-14 2017-10-05 Inguran, Llc Apparatus and methods for high throughput sperm sorting
US10371622B2 (en) 2013-03-14 2019-08-06 Inguran, Llc Device for high throughput sperm sorting
US10662408B2 (en) 2013-03-14 2020-05-26 Inguran, Llc Methods for high throughput sperm sorting
US9757726B2 (en) 2013-03-14 2017-09-12 Inguran, Llc System for high throughput sperm sorting
GB201512600D0 (en) * 2015-07-17 2015-08-26 Koniku Ltd Cell culture, transport and investigation
US11474103B2 (en) 2017-02-17 2022-10-18 Koniku Inc. Systems for detection
KR101959208B1 (ko) * 2017-05-24 2019-03-19 한국기계연구원 액적기반 바이오칩, 이를 이용한 종양 생산방법, 및 이를 통해 생산된 종양의 전이성 판단방법
US11477970B2 (en) 2018-02-21 2022-10-25 Nemametrix Inc. Transgenic animal phenotyping platform and uses thereof
JP7539836B2 (ja) * 2018-04-04 2024-08-26 ザ ジェネラル ホスピタル コーポレイション 哺乳類卵母細胞を裸化するためのマイクロ流体システムおよび方法
WO2020005802A1 (fr) * 2018-06-24 2020-01-02 University Of Oregon Puce de micro-injection, dispositif, système et leurs utilisations pour les organismes monocellulaires ou multicellulaires
FR3084121B1 (fr) * 2018-07-17 2021-01-15 Skf Aerospace France Assemblage mecanique et dispositif mecanique
JP2022502060A (ja) 2018-09-28 2022-01-11 オーバーチュア ライフ，インコーポレイテッド 体外受精システム及びそれに関連する構成要素
CN111073817A (zh) * 2018-10-22 2020-04-28 曾繁根 用于精子分选、卵子培育、体外受精的整合式晶片及方法
CN111378556A (zh) * 2018-12-29 2020-07-07 青岛华大智造普惠科技有限公司 微流控芯片及其制备方法、单细胞微液滴的制备方法
WO2020242860A1 (fr) * 2019-05-24 2020-12-03 University Of Houston System Appareil et méthodes destinés à des applications médicales de pompes microfluidiques commandées par laser
KR102267433B1 (ko) * 2019-07-29 2021-06-21 연세대학교 산학협력단 세포배양 플레이트
KR102243812B1 (ko) * 2019-08-13 2021-04-22 경희대학교 산학협력단 체외수정 자동화 장치 및 그것의 동작 방법
DE102020214862B4 (de) * 2020-11-26 2022-08-04 Rheinisch-Westfälische Technische Hochschule (RWTH) Aachen, Körperschaft des öffentlichen Rechts System und Vorrichtung zur Charakterisierung von Veränderungen des Reifungsvorgangs einer Eizelle, sowie deren Verwendung
EP4689069A1 (fr) * 2023-04-03 2026-02-11 College de France Dispositif microfluidique destiné à être utilisé dans un système de mesure d'au moins une propriété mécanique d'au moins un ovocyte
CN117106561A (zh) * 2023-09-01 2023-11-24 杭州澳麒生物科技有限公司 一种用于高效过滤细胞的微流控芯片及制备方法和应用
CN117660183B (zh) * 2024-01-31 2024-04-16 山东大学 胚胎微流控动态培养皿及其培养方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU677780B2 (en) * 1992-05-01 1997-05-08 Trustees Of The University Of Pennsylvania, The Microfabricated detection structures
ATE530891T1 (de) * 1998-05-22 2011-11-15 California Inst Of Techn Miniaturisierter zellsortierer
US6193647B1 (en) * 1999-04-08 2001-02-27 The Board Of Trustees Of The University Of Illinois Microfluidic embryo and/or oocyte handling device and method

Also Published As

Publication number Publication date
AU2001259635B2 (en) 2005-05-19
JP2003533221A (ja) 2003-11-11
AU5963501A (en) 2001-11-26
IL152650A0 (en) 2003-06-24
RU2002130577A (ru) 2004-03-27
WO2001088087A2 (fr) 2001-11-22
BR0110768A (pt) 2003-03-11
IL152650A (en) 2006-12-31
WO2001088087A3 (fr) 2002-03-28
KR20030009482A (ko) 2003-01-29
CA2408616A1 (fr) 2001-11-22
EP1280465A4 (fr) 2004-10-20
EP1280465A2 (fr) 2003-02-05
CN1441652A (zh) 2003-09-10
MXPA02011150A (es) 2004-08-19

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