CA2430580A1 - Domaine specialise d'activites jumelees de condensation et d'epimerisation dans des systemes de peptides synthetases non ribosomiques - Google Patents

Domaine specialise d'activites jumelees de condensation et d'epimerisation dans des systemes de peptides synthetases non ribosomiques Download PDF

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Publication number
CA2430580A1
CA2430580A1 CA002430580A CA2430580A CA2430580A1 CA 2430580 A1 CA2430580 A1 CA 2430580A1 CA 002430580 A CA002430580 A CA 002430580A CA 2430580 A CA2430580 A CA 2430580A CA 2430580 A1 CA2430580 A1 CA 2430580A1
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CA
Canada
Prior art keywords
leu
ala
arg
val
asp
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002430580A
Other languages
English (en)
Inventor
Chris M. Farnet
Alfredo Staffa
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Thallion Pharmaceuticals Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2430580A1 publication Critical patent/CA2430580A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/04—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers
    • C07K1/047—Simultaneous synthesis of different peptide species; Peptide libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

L'invention porte sur des domaines de synthétases de peptides non ribosomiques (NRPS) qui présentent des activités de double condensation et épimérisation. Ces </= domaines de double condensation/epimérisation NRPS >/= permettent l'introduction de substrats non protéinogéniques, tels que les acides aminés D, dans des produits peptidiques. Ces domaines de double condensation/epimérisation NRPS peuvent également servir à modifier la stéréochimie de peptides synthétisés au niveau des sites d'acides aminés sélectionnés.
CA002430580A 2002-04-17 2003-04-17 Domaine specialise d'activites jumelees de condensation et d'epimerisation dans des systemes de peptides synthetases non ribosomiques Abandoned CA2430580A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US37279002P 2002-04-17 2002-04-17
US60/372,790 2002-04-17
PCT/CA2003/000575 WO2003089641A2 (fr) 2002-04-17 2003-04-17 Domaine de double condensation/epimerisation dans des systemes de synthetases de peptides non ribosomiques

Publications (1)

Publication Number Publication Date
CA2430580A1 true CA2430580A1 (fr) 2003-09-04

Family

ID=27805336

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002430580A Abandoned CA2430580A1 (fr) 2002-04-17 2003-04-17 Domaine specialise d'activites jumelees de condensation et d'epimerisation dans des systemes de peptides synthetases non ribosomiques

Country Status (3)

Country Link
AU (1) AU2003222690A1 (fr)
CA (1) CA2430580A1 (fr)
WO (1) WO2003089641A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114657155A (zh) * 2021-12-23 2022-06-24 中国农业科学院生物技术研究所 一种新型的非核糖体多肽合成酶拆分表达方法

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE292684T1 (de) * 2000-10-13 2005-04-15 Ecopia Biosciences Inc Ramoplaninbiosynthesegenkluster
CN117384924B (zh) * 2023-12-11 2024-06-25 中国农业科学院生物技术研究所 一种基于酶结构域拆分的多肽生物合成方法

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ZA801629B (en) * 1979-04-07 1981-03-25 Lepetit Spa Antibiotic a/16686 and process for the preparation thereof

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114657155A (zh) * 2021-12-23 2022-06-24 中国农业科学院生物技术研究所 一种新型的非核糖体多肽合成酶拆分表达方法
CN114657155B (zh) * 2021-12-23 2024-01-19 中国农业科学院生物技术研究所 一种新型的非核糖体多肽合成酶拆分表达方法

Also Published As

Publication number Publication date
WO2003089641A3 (fr) 2004-06-10
AU2003222690A1 (en) 2003-11-03
WO2003089641A2 (fr) 2003-10-30

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