CA2471874A1 - Compositions et methodes d'hydroxylation d'epothilones - Google Patents

Compositions et methodes d'hydroxylation d'epothilones Download PDF

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Publication number
CA2471874A1
CA2471874A1 CA002471874A CA2471874A CA2471874A1 CA 2471874 A1 CA2471874 A1 CA 2471874A1 CA 002471874 A CA002471874 A CA 002471874A CA 2471874 A CA2471874 A CA 2471874A CA 2471874 A1 CA2471874 A1 CA 2471874A1
Authority
CA
Canada
Prior art keywords
epothilone
seq
nucleic acid
acid sequence
hydroxylase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002471874A
Other languages
English (en)
Inventor
Jonathan David Basch
Shu-Jen Chiang
Suo-Win Liu
Akbar Nayeem
Yuhua Sun
You Li
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bristol Myers Squibb Co
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2471874A1 publication Critical patent/CA2471874A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0077—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with a reduced iron-sulfur protein as one donor (1.14.15)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/79—Transferrins, e.g. lactoferrins, ovotransferrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/18—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
    • C12P17/181—Heterocyclic compounds containing oxygen atoms as the only ring heteroatoms in the condensed system, e.g. Salinomycin, Septamycin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2299/00—Coordinates from 3D structures of peptides, e.g. proteins or enzymes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Communicable Diseases (AREA)
  • Animal Behavior & Ethology (AREA)
  • Toxicology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Oncology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)

Abstract

La présente invention concerne des séquences d'acide nucléiques isolées et des polypeptides codés par ces dernières qui produisent de l'épothilone B hydroxylase ainsi que des mutants et des variants de cette dernière et une ferrédoxine située en aval du gène d'épothilone B hydroxylase. Cette invention concerne également des vecteurs et des cellules contenant ces vecteurs; des méthodes de production de micro-organismes de recombinaison, des méthodes d'utilisation de ces micro-organismes de recombinaison pour produire des épothilones porteuses d'un groupe hydroxyalkyle et un analogue d'épothilone produit par un mutant de l'épothilone B hydroxylase.
CA002471874A 2001-12-26 2002-12-17 Compositions et methodes d'hydroxylation d'epothilones Abandoned CA2471874A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US34427101P 2001-12-26 2001-12-26
US60/344,271 2001-12-26
PCT/US2002/040359 WO2003057830A2 (fr) 2001-12-26 2002-12-17 Compositions et methodes d'hydroxylation d'epothilones

Publications (1)

Publication Number Publication Date
CA2471874A1 true CA2471874A1 (fr) 2003-07-17

Family

ID=23349792

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002471874A Abandoned CA2471874A1 (fr) 2001-12-26 2002-12-17 Compositions et methodes d'hydroxylation d'epothilones

Country Status (6)

Country Link
EP (1) EP1465908A4 (fr)
JP (1) JP2005514031A (fr)
AU (1) AU2002357872A1 (fr)
CA (1) CA2471874A1 (fr)
TW (1) TW200301697A (fr)
WO (1) WO2003057830A2 (fr)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6204388B1 (en) 1996-12-03 2001-03-20 Sloan-Kettering Institute For Cancer Research Synthesis of epothilones, intermediates thereto and analogues thereof
CA2273083C (fr) 1996-12-03 2012-09-18 Sloan-Kettering Institute For Cancer Research Synthese d'epothilones, intermediaires utilises dans leur synthese, analogues et utilisations de celles-ci
US6884608B2 (en) * 2001-12-26 2005-04-26 Bristol-Myers Squibb Company Compositions and methods for hydroxylating epothilones
US20060234337A1 (en) * 2002-04-12 2006-10-19 Akira Arisawa Expression system for actinomycete-origin cytochrome p-450 in escherichia coli
US11384371B2 (en) 2016-11-15 2022-07-12 Hypha Discovery Limited Hydroxylation of branched aliphatic or aromatic substrates employing the amycolatopsis lurida cytochrome P450
GB201807815D0 (en) 2018-05-14 2018-06-27 Hypha Discovery Ltd Hydroxylation techniques
GB201819209D0 (en) 2018-11-26 2019-01-09 Hypha Discovery Ltd Biocatalytic techniques
GB201917077D0 (en) 2019-11-22 2020-01-08 Hypha Discovery Ltd Biocatalytic techniques

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20010092453A (ko) * 1998-12-23 2001-10-25 스티븐 비. 데이비스 에포틸론 제조를 위한 미생물적 전환 방법
US7070964B2 (en) * 2001-11-15 2006-07-04 Kosan Biosciences Incorporated Epothilone compounds and methods for making the same

Also Published As

Publication number Publication date
TW200301697A (en) 2003-07-16
EP1465908A4 (fr) 2005-11-30
WO2003057830A2 (fr) 2003-07-17
JP2005514031A (ja) 2005-05-19
AU2002357872A1 (en) 2003-07-24
EP1465908A2 (fr) 2004-10-13
WO2003057830A3 (fr) 2004-08-05

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