CA2495198C - Nouvelles lipases et utilisations - Google Patents

Nouvelles lipases et utilisations Download PDF

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Publication number
CA2495198C
CA2495198C CA2495198A CA2495198A CA2495198C CA 2495198 C CA2495198 C CA 2495198C CA 2495198 A CA2495198 A CA 2495198A CA 2495198 A CA2495198 A CA 2495198A CA 2495198 C CA2495198 C CA 2495198C
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CA
Canada
Prior art keywords
leu
ala
gly
ser
val
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
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CA2495198A
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English (en)
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CA2495198A1 (fr
Inventor
Richard Albang
Ulrike Folkers
Andreas Fritz
Beatrix Gerhard
Oliver Heinrich
Hilmar Ilgenfritz
Dieter Maier
Fabio Spreafico
Christian Wagner
Lex De Boer
Roelf Bernhard Meima
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DSM IP Assets BV
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DSM IP Assets BV
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Publication of CA2495198A1 publication Critical patent/CA2495198A1/fr
Application granted granted Critical
Publication of CA2495198C publication Critical patent/CA2495198C/fr
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Bakery Products And Manufacturing Methods Therefor (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La présente invention concerne une séquence de polynucléotides nouvellement identifiée qui comprend un gène codant une nouvelle enzyme lipolytique issue de Aspergillus niger. L'invention se rapporte à une séquence nucléotidique de pleine longueur du nouveau gène, à la séquence d'ADNc comprenant la séquence de codage de pleine longueur de la nouvelle enzyme lipolytique, ainsi qu'à la séquence d'acides aminés de la protéine fonctionnelle de pleine longueur et aux équivalents fonctionnels de cette dernière. L'invention concerne aussi des procédés d'utilisation des enzymes précitées dans des processus industriels, et des procédés de diagnostic d'infections fongiques. L'invention porte enfin sur des cellules transformées au moyen d'un polynucléotide de l'invention et sur des cellules dans lesquelles on a génétiquement modifié une enzyme lipolytique de l'invention afin d'augmenter son activité et/ou son niveau d'expression.
CA2495198A 2002-08-19 2003-08-15 Nouvelles lipases et utilisations Expired - Fee Related CA2495198C (fr)

Applications Claiming Priority (25)

Application Number Priority Date Filing Date Title
EP02102171 2002-08-19
EP02102174 2002-08-19
EP02102178.7 2002-08-19
EP02102173 2002-08-19
EP02102179 2002-08-19
EP02102179.5 2002-08-19
EP02102169 2002-08-19
EP02102171.2 2002-08-19
EP02102181 2002-08-19
EP02102172.0 2002-08-19
EP02102176.1 2002-08-19
EP02102174.6 2002-08-19
EP02102183 2002-08-19
EP02102172 2002-08-19
EP02102168 2002-08-19
EP02102178 2002-08-19
EP02102183.7 2002-08-19
EP02102173.8 2002-08-19
EP02102169.6 2002-08-19
EP02102181.1 2002-08-19
EP02102170 2002-08-19
EP02102176 2002-08-19
EP02102168.8 2002-08-19
EP02102170.4 2002-08-19
PCT/EP2003/009145 WO2004018660A2 (fr) 2002-08-19 2003-08-15 Nouvelles lipases et utilisations

Publications (2)

Publication Number Publication Date
CA2495198A1 CA2495198A1 (fr) 2004-03-04
CA2495198C true CA2495198C (fr) 2014-03-11

Family

ID=31951005

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2495198A Expired - Fee Related CA2495198C (fr) 2002-08-19 2003-08-15 Nouvelles lipases et utilisations

Country Status (13)

Country Link
US (2) US7550280B2 (fr)
EP (2) EP2338986B1 (fr)
JP (1) JP2005535352A (fr)
CN (1) CN1675355A (fr)
AR (1) AR042417A1 (fr)
AU (1) AU2003270088A1 (fr)
CA (1) CA2495198C (fr)
DK (1) DK2338986T3 (fr)
EA (2) EA008007B1 (fr)
ES (1) ES2527924T3 (fr)
MX (1) MXPA05001978A (fr)
PL (2) PL214792B1 (fr)
WO (1) WO2004018660A2 (fr)

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ATE231186T1 (de) 1998-07-21 2003-02-15 Danisco Lebensmittel
BR0209154A (pt) 2001-05-18 2004-07-20 Danisco Processo de preparação de uma massa com uma enzima
CN1675355A (zh) * 2002-08-19 2005-09-28 帝斯曼知识产权资产管理有限公司 新颖的脂肪酶及其用途
US7955814B2 (en) 2003-01-17 2011-06-07 Danisco A/S Method
US20050196766A1 (en) 2003-12-24 2005-09-08 Soe Jorn B. Proteins
DE602004030000D1 (de) 2003-01-17 2010-12-23 Danisco Verfahren zur in-situ-herstellung eines emulgators in einem nahrungsmittel
WO2008090395A1 (fr) 2007-01-25 2008-07-31 Danisco A/S Production d'une lipide acyltransférase à partir de cellules transformées de bacillus licheniformis
GB0716126D0 (en) 2007-08-17 2007-09-26 Danisco Process
US7906307B2 (en) 2003-12-24 2011-03-15 Danisco A/S Variant lipid acyltransferases and methods of making
US7718408B2 (en) 2003-12-24 2010-05-18 Danisco A/S Method
GB0405637D0 (en) 2004-03-12 2004-04-21 Danisco Protein
CN101052702B (zh) 2004-07-16 2013-01-09 杜邦营养生物科学有限公司 脂肪分解酶及其在食品工业中的应用
EP1812566A2 (fr) 2004-10-21 2007-08-01 Novozymes, Inc. Polypeptides a activite lipase et polynucleotides les codant
CN101389644B (zh) * 2006-02-23 2015-06-17 帝斯曼知识产权资产管理有限公司 脂肪酶及其用途
EP2119781B1 (fr) 2007-01-30 2012-08-22 Mitsubishi-Kagaku Foods Corporation Lipase glyceroglycolipidique
MX2010009072A (es) * 2008-02-29 2010-09-24 Novozymes As Polipeptidos que tienen actividad de lipasa y polinucleotidos que codifican para los mismos.
EP2387330B1 (fr) 2009-01-16 2017-11-15 DuPont Nutrition Biosciences ApS Génération enzymatique de lipides fonctionnels dans de son de céréales
WO2010130622A1 (fr) * 2009-05-11 2010-11-18 Dsm Ip Assets B.V. Composition enzymatique pour le traitement de l'insuffisance pancréatique
KR101724614B1 (ko) * 2014-05-27 2017-04-19 주식회사 제노포커스 신규 카탈라아제 신호서열 및 이를 이용한 카탈라아제 발현방법
US11229214B2 (en) 2016-11-30 2022-01-25 Novozymes A/S Method of baking
CN113301812A (zh) * 2018-10-17 2021-08-24 完美日股份有限公司 用于食物产品的重组组分和组合物
EP4511501A1 (fr) 2022-04-20 2025-02-26 Novozymes A/S Procede de production d'acides gras libres
EP4420520A1 (fr) * 2023-02-24 2024-08-28 Green Spot Technologies Panification avec des b-glucanes et de la laccase ou de la peroxydase
CN117925570B (zh) * 2023-12-18 2025-04-22 蚌埠学院 从羊胎盘中提取生物活性物质的方法

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Also Published As

Publication number Publication date
DK2338986T3 (en) 2015-01-19
EA200601823A1 (ru) 2007-02-27
PL375355A1 (en) 2005-11-28
AR042417A1 (es) 2005-06-22
WO2004018660A3 (fr) 2004-10-28
US7550280B2 (en) 2009-06-23
ES2527924T3 (es) 2015-02-02
EP2338986B1 (fr) 2014-10-29
US20060281080A1 (en) 2006-12-14
MXPA05001978A (es) 2005-04-28
US8216586B2 (en) 2012-07-10
WO2004018660A2 (fr) 2004-03-04
JP2005535352A (ja) 2005-11-24
EA200500391A1 (ru) 2005-08-25
US20090232936A1 (en) 2009-09-17
PL214792B1 (pl) 2013-09-30
AU2003270088A1 (en) 2004-03-11
EA008007B1 (ru) 2007-02-27
CA2495198A1 (fr) 2004-03-04
EP2338986A1 (fr) 2011-06-29
CN1675355A (zh) 2005-09-28
EP1530630A2 (fr) 2005-05-18

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Effective date: 20170815