CA2496251A1 - Dosage a haut rendement de proliferation de souches hematopoietiques et de cellules progenitrices - Google Patents
Dosage a haut rendement de proliferation de souches hematopoietiques et de cellules progenitrices Download PDFInfo
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- CA2496251A1 CA2496251A1 CA002496251A CA2496251A CA2496251A1 CA 2496251 A1 CA2496251 A1 CA 2496251A1 CA 002496251 A CA002496251 A CA 002496251A CA 2496251 A CA2496251 A CA 2496251A CA 2496251 A1 CA2496251 A1 CA 2496251A1
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5082—Supracellular entities, e.g. tissue, organisms
- G01N33/5088—Supracellular entities, e.g. tissue, organisms of vertebrates
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- Life Sciences & Earth Sciences (AREA)
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Abstract
La présente invention concerne d'une façon générale des kits qui fournissent des mélanges de réactifs et des instructions d'utilisation de ceux-ci dans des techniques de dosage à haut rendement qui déterminent l'état de prolifération de populations de cellules cibles isolées. Ces techniques mesurent la sortie luminescente provenant du contenu ATP intracellulaire de cellules cibles incubées et établissent une corrélation entre cette luminescence et l'état de prolifération de ces cellules. Cette invention concerne aussi des kits qui fournissent des mélanges de réactifs et des instructions destinés à des techniques de dosage à haut rendement permettant de rechercher des composés qui peuvent moduler l'état de prolifération d'une population de cellules cible. Les kits de cette invention et les techniques décrites peuvent être utilisés pour déterminer l'état de prolifération de n'importe quelle lignée ou type de cellules isolées. Les kits et les procédés de cette invention répondent au besoin de dosages rapides qui puissent déterminer l'état de prolifération de cellules hématopoïétiques et de cellules progénitrices isolées et de sous populations de cellules différenciées de celles-ci.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US40497202P | 2002-08-21 | 2002-08-21 | |
| US60/404,972 | 2002-08-21 | ||
| PCT/US2003/026167 WO2004018996A2 (fr) | 2002-08-21 | 2003-08-21 | Dosage a haut rendement de proliferation de souches hematopoietiques et de cellules progenitrices |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2496251A1 true CA2496251A1 (fr) | 2004-03-04 |
Family
ID=31946791
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002496251A Abandoned CA2496251A1 (fr) | 2002-08-21 | 2003-08-21 | Dosage a haut rendement de proliferation de souches hematopoietiques et de cellules progenitrices |
Country Status (4)
| Country | Link |
|---|---|
| EP (1) | EP1539997A4 (fr) |
| AU (1) | AU2003258319B2 (fr) |
| CA (1) | CA2496251A1 (fr) |
| WO (1) | WO2004018996A2 (fr) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7989178B2 (en) | 2001-01-29 | 2011-08-02 | Hemogenix, Inc. | Colony assay miniaturization with enumeration output |
| EP1364197B1 (fr) | 2001-01-29 | 2010-04-14 | Ivan N. Rich | Essai basé sur l'ATP pour la détermination de la prolifération de cellules hématopoietiques souches et progénitrices |
| US7666615B2 (en) | 2001-01-29 | 2010-02-23 | Hemogenix, Inc. | High-throughput assay of hematopoietic stem and progenitor cell proliferation |
| US7354730B2 (en) | 2002-01-29 | 2008-04-08 | Hemogenix, Inc. | High-throughput assay of hematopoietic stem and progenitor cell proliferation |
| CN103827667B (zh) * | 2011-09-13 | 2016-07-13 | 巴斯夫欧洲公司 | 评估造血毒性的工具和方法 |
| EP4417210A1 (fr) * | 2017-03-15 | 2024-08-21 | Orca Biosystems, Inc. | Compositions et procédés pour greffes de cellules souches hématopoïétiques |
| WO2021013669A1 (fr) * | 2019-07-24 | 2021-01-28 | Miltenyi Biotec B.V. & Co. KG | Dosage permettant d'identifier des cellules formant des colonies |
| CN115927169B (zh) * | 2022-10-11 | 2023-08-11 | 再造再生医学科技(杭州)有限公司 | 用于扩增cd34+造血干细胞的培养液和体外扩增cd34+造血干细胞的方法 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5283179A (en) * | 1990-09-10 | 1994-02-01 | Promega Corporation | Luciferase assay method |
| US5733541A (en) * | 1995-04-21 | 1998-03-31 | The Regent Of The University Of Michigan | Hematopoietic cells: compositions and methods |
| JPH10136978A (ja) * | 1996-11-08 | 1998-05-26 | Otsuka Pharmaceut Co Ltd | 造血幹細胞の培養方法 |
| US5854010A (en) * | 1997-03-10 | 1998-12-29 | Denison; Michael S. | Bioassay for detecting 2,3,7,8-tetrachlorodibenzo-para-dioxin and TCDD-like compounds and novel recombinant cell line useful therefor |
| EP1364197B1 (fr) * | 2001-01-29 | 2010-04-14 | Ivan N. Rich | Essai basé sur l'ATP pour la détermination de la prolifération de cellules hématopoietiques souches et progénitrices |
-
2003
- 2003-08-21 AU AU2003258319A patent/AU2003258319B2/en not_active Ceased
- 2003-08-21 WO PCT/US2003/026167 patent/WO2004018996A2/fr not_active Ceased
- 2003-08-21 CA CA002496251A patent/CA2496251A1/fr not_active Abandoned
- 2003-08-21 EP EP03793209A patent/EP1539997A4/fr not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| WO2004018996A2 (fr) | 2004-03-04 |
| EP1539997A4 (fr) | 2007-04-25 |
| AU2003258319A1 (en) | 2004-03-11 |
| EP1539997A2 (fr) | 2005-06-15 |
| AU2003258319B2 (en) | 2009-09-17 |
| WO2004018996A3 (fr) | 2004-06-10 |
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| EEER | Examination request | ||
| FZDE | Discontinued |