WO2004018996A3 - Dosage a haut rendement de proliferation de souches hematopoietiques et de cellules progenitrices - Google Patents

Dosage a haut rendement de proliferation de souches hematopoietiques et de cellules progenitrices Download PDF

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Publication number
WO2004018996A3
WO2004018996A3 PCT/US2003/026167 US0326167W WO2004018996A3 WO 2004018996 A3 WO2004018996 A3 WO 2004018996A3 US 0326167 W US0326167 W US 0326167W WO 2004018996 A3 WO2004018996 A3 WO 2004018996A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
kits
proliferative status
present
cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2003/026167
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English (en)
Other versions
WO2004018996A2 (fr
Inventor
Ivan N Rich
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to EP03793209A priority Critical patent/EP1539997A4/fr
Priority to AU2003258319A priority patent/AU2003258319B2/en
Priority to CA002496251A priority patent/CA2496251A1/fr
Publication of WO2004018996A2 publication Critical patent/WO2004018996A2/fr
Publication of WO2004018996A3 publication Critical patent/WO2004018996A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5082—Supracellular entities, e.g. tissue, organisms
    • G01N33/5088—Supracellular entities, e.g. tissue, organisms of vertebrates

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Food Science & Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biophysics (AREA)
  • Toxicology (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention concerne d'une façon générale des kits qui fournissent des mélanges de réactifs et des instructions d'utilisation de ceux-ci dans des techniques de dosage à haut rendement qui déterminent l'état de prolifération de populations de cellules cibles isolées. Ces techniques mesurent la sortie luminescente provenant du contenu ATP intracellulaire de cellules cibles incubées et établissent une corrélation entre cette luminescence et l'état de prolifération de ces cellules. Cette invention concerne aussi des kits qui fournissent des mélanges de réactifs et des instructions destinés à des techniques de dosage à haut rendement permettant de rechercher des composés qui peuvent moduler l'état de prolifération d'une population de cellules cible. Les kits de cette invention et les techniques décrites peuvent être utilisés pour déterminer l'état de prolifération de n'importe quelle lignée ou type de cellules isolées. Les kits et les procédés de cette invention répondent au besoin de dosages rapides qui puissent déterminer l'état de prolifération de cellules hématopoïétiques et de cellules progénitrices isolées et de sous populations de cellules différenciées de celles-ci.
PCT/US2003/026167 2002-08-21 2003-08-21 Dosage a haut rendement de proliferation de souches hematopoietiques et de cellules progenitrices Ceased WO2004018996A2 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP03793209A EP1539997A4 (fr) 2002-08-21 2003-08-21 Dosage a haut rendement de proliferation de souches hematopoietiques et de cellules progenitrices
AU2003258319A AU2003258319B2 (en) 2002-08-21 2003-08-21 High-throughput assay of hematopoietic stem and progenitor cell proliferation
CA002496251A CA2496251A1 (fr) 2002-08-21 2003-08-21 Dosage a haut rendement de proliferation de souches hematopoietiques et de cellules progenitrices

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US40497202P 2002-08-21 2002-08-21
US60/404,972 2002-08-21

Publications (2)

Publication Number Publication Date
WO2004018996A2 WO2004018996A2 (fr) 2004-03-04
WO2004018996A3 true WO2004018996A3 (fr) 2004-06-10

Family

ID=31946791

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/026167 Ceased WO2004018996A2 (fr) 2002-08-21 2003-08-21 Dosage a haut rendement de proliferation de souches hematopoietiques et de cellules progenitrices

Country Status (4)

Country Link
EP (1) EP1539997A4 (fr)
AU (1) AU2003258319B2 (fr)
CA (1) CA2496251A1 (fr)
WO (1) WO2004018996A2 (fr)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7989178B2 (en) 2001-01-29 2011-08-02 Hemogenix, Inc. Colony assay miniaturization with enumeration output
EP1364197B1 (fr) 2001-01-29 2010-04-14 Ivan N. Rich Essai basé sur l'ATP pour la détermination de la prolifération de cellules hématopoietiques souches et progénitrices
US7666615B2 (en) 2001-01-29 2010-02-23 Hemogenix, Inc. High-throughput assay of hematopoietic stem and progenitor cell proliferation
US7354730B2 (en) 2002-01-29 2008-04-08 Hemogenix, Inc. High-throughput assay of hematopoietic stem and progenitor cell proliferation
CN103827667B (zh) * 2011-09-13 2016-07-13 巴斯夫欧洲公司 评估造血毒性的工具和方法
EP4417210A1 (fr) * 2017-03-15 2024-08-21 Orca Biosystems, Inc. Compositions et procédés pour greffes de cellules souches hématopoïétiques
WO2021013669A1 (fr) * 2019-07-24 2021-01-28 Miltenyi Biotec B.V. & Co. KG Dosage permettant d'identifier des cellules formant des colonies
CN115927169B (zh) * 2022-10-11 2023-08-11 再造再生医学科技(杭州)有限公司 用于扩增cd34+造血干细胞的培养液和体外扩增cd34+造血干细胞的方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5641641A (en) * 1990-09-10 1997-06-24 Promega Corporation Kit for luciferase assay
US5733541A (en) * 1995-04-21 1998-03-31 The Regent Of The University Of Michigan Hematopoietic cells: compositions and methods
US5854010A (en) * 1997-03-10 1998-12-29 Denison; Michael S. Bioassay for detecting 2,3,7,8-tetrachlorodibenzo-para-dioxin and TCDD-like compounds and novel recombinant cell line useful therefor

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH10136978A (ja) * 1996-11-08 1998-05-26 Otsuka Pharmaceut Co Ltd 造血幹細胞の培養方法
EP1364197B1 (fr) * 2001-01-29 2010-04-14 Ivan N. Rich Essai basé sur l'ATP pour la détermination de la prolifération de cellules hématopoietiques souches et progénitrices

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5641641A (en) * 1990-09-10 1997-06-24 Promega Corporation Kit for luciferase assay
US5814471A (en) * 1990-09-10 1998-09-29 Promega Corporation Luciferase inhibitor compositions and methods of using same
US5733541A (en) * 1995-04-21 1998-03-31 The Regent Of The University Of Michigan Hematopoietic cells: compositions and methods
US5854010A (en) * 1997-03-10 1998-12-29 Denison; Michael S. Bioassay for detecting 2,3,7,8-tetrachlorodibenzo-para-dioxin and TCDD-like compounds and novel recombinant cell line useful therefor

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP1539997A4 *

Also Published As

Publication number Publication date
WO2004018996A2 (fr) 2004-03-04
EP1539997A4 (fr) 2007-04-25
AU2003258319A1 (en) 2004-03-11
EP1539997A2 (fr) 2005-06-15
AU2003258319B2 (en) 2009-09-17
CA2496251A1 (fr) 2004-03-04

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