CA2534441A1 - Jeux ordonnes d'echantillons, procedes et kits de preparation et d'utilisation de jeux ordonnes d'echantillons genomiques synteniques pour l'etablissement de diagnostics et la toxicologie - Google Patents

Jeux ordonnes d'echantillons, procedes et kits de preparation et d'utilisation de jeux ordonnes d'echantillons genomiques synteniques pour l'etablissement de diagnostics et la toxicologie Download PDF

Info

Publication number: CA2534441A1
Authority: CA; Canada
Prior art keywords: nucleic acid; array; species; elements; syntenic
Prior art date: 2003-08-01
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Abandoned

Application number

CA002534441A

Other languages

English (en)

Inventor

Mansoor Mohammed

Robert C. Johnson

Jae Weon Kim

Xan-Yan Lu

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Individual

Original Assignee

Spectral Genomics Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2003-08-01

Filing date

2004-08-02

Publication date

2005-02-10

2004-08-02 Application filed by Spectral Genomics Inc filed Critical Spectral Genomics Inc

2005-02-10 Publication of CA2534441A1 publication Critical patent/CA2534441A1/fr

Status Abandoned legal-status Critical Current

Links

238000000034 method Methods 0.000 title claims abstract description 158
238000003491 array Methods 0.000 title claims abstract description 93
238000002360 preparation method Methods 0.000 title claims description 10
231100000027 toxicology Toxicity 0.000 title description 7
238000009396 hybridization Methods 0.000 claims abstract description 147
210000004027 cell Anatomy 0.000 claims abstract description 146
210000000349 chromosome Anatomy 0.000 claims abstract description 95
206010028980 Neoplasm Diseases 0.000 claims abstract description 44
201000011510 cancer Diseases 0.000 claims abstract description 30
230000005856 abnormality Effects 0.000 claims abstract description 26
150000007523 nucleic acids Chemical group 0.000 claims description 262
102000039446 nucleic acids Human genes 0.000 claims description 239
108020004707 nucleic acids Proteins 0.000 claims description 239
239000000523 sample Substances 0.000 claims description 139
108020004414 DNA Proteins 0.000 claims description 123
241000894007 species Species 0.000 claims description 115
239000000203 mixture Substances 0.000 claims description 109
238000012360 testing method Methods 0.000 claims description 105
201000010099 disease Diseases 0.000 claims description 49
208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 49
239000000758 substrate Substances 0.000 claims description 48
239000000975 dye Substances 0.000 claims description 46
208000031404 Chromosome Aberrations Diseases 0.000 claims description 42
241000699666 Mus <mouse, genus> Species 0.000 claims description 38
241001465754 Metazoa Species 0.000 claims description 36
239000013074 reference sample Substances 0.000 claims description 26
206010008805 Chromosomal abnormalities Diseases 0.000 claims description 24
239000007850 fluorescent dye Substances 0.000 claims description 23
238000002372 labelling Methods 0.000 claims description 20
210000004436 artificial bacterial chromosome Anatomy 0.000 claims description 19
230000001738 genotoxic effect Effects 0.000 claims description 17
230000002759 chromosomal effect Effects 0.000 claims description 15
238000010171 animal model Methods 0.000 claims description 12
210000001106 artificial yeast chromosome Anatomy 0.000 claims description 12
210000004507 artificial chromosome Anatomy 0.000 claims description 11
230000004888 barrier function Effects 0.000 claims description 11
231100000025 genetic toxicology Toxicity 0.000 claims description 10
230000009261 transgenic effect Effects 0.000 claims description 10
108091028043 Nucleic acid sequence Proteins 0.000 claims description 9
241000283984 Rodentia Species 0.000 claims description 9
210000004369 blood Anatomy 0.000 claims description 9
239000008280 blood Substances 0.000 claims description 9
210000000723 mammalian artificial chromosome Anatomy 0.000 claims description 9
238000003499 nucleic acid array Methods 0.000 claims description 9
239000013598 vector Substances 0.000 claims description 9
241000124008 Mammalia Species 0.000 claims description 7
241000700159 Rattus Species 0.000 claims description 7
238000004113 cell culture Methods 0.000 claims description 7
241000251468 Actinopterygii Species 0.000 claims description 6
241000283690 Bos taurus Species 0.000 claims description 6
241000283073 Equus caballus Species 0.000 claims description 6
150000001875 compounds Chemical class 0.000 claims description 6
238000001727 in vivo Methods 0.000 claims description 6
241000700199 Cavia porcellus Species 0.000 claims description 5
241000238631 Hexapoda Species 0.000 claims description 5
206010058467 Lung neoplasm malignant Diseases 0.000 claims description 5
241000282577 Pan troglodytes Species 0.000 claims description 5
206010060862 Prostate cancer Diseases 0.000 claims description 5
208000000236 Prostatic Neoplasms Diseases 0.000 claims description 5
208000009956 adenocarcinoma Diseases 0.000 claims description 5
201000005202 lung cancer Diseases 0.000 claims description 5
208000020816 lung neoplasm Diseases 0.000 claims description 5
241000283707 Capra Species 0.000 claims description 4
241000282693 Cercopithecidae Species 0.000 claims description 4
241000699800 Cricetinae Species 0.000 claims description 4
241000702191 Escherichia virus P1 Species 0.000 claims description 4
241000282575 Gorilla Species 0.000 claims description 4
206010027406 Mesothelioma Diseases 0.000 claims description 4
241000283973 Oryctolagus cuniculus Species 0.000 claims description 4
241001494479 Pecora Species 0.000 claims description 4
241000282898 Sus scrofa Species 0.000 claims description 4
238000000295 emission spectrum Methods 0.000 claims description 3
230000007613 environmental effect Effects 0.000 claims description 3
241000269328 Amphibia Species 0.000 claims description 2
241000283153 Cetacea Species 0.000 claims description 2
241000252212 Danio rerio Species 0.000 claims description 2
241000283953 Lagomorpha Species 0.000 claims description 2
206010025323 Lymphomas Diseases 0.000 claims description 2
241000009328 Perro Species 0.000 claims description 2
210000004556 brain Anatomy 0.000 claims description 2
210000000481 breast Anatomy 0.000 claims description 2
239000002575 chemical warfare agent Substances 0.000 claims description 2
239000002537 cosmetic Substances 0.000 claims description 2
239000000428 dust Substances 0.000 claims description 2
239000000284 extract Substances 0.000 claims description 2
235000013373 food additive Nutrition 0.000 claims description 2
239000002778 food additive Substances 0.000 claims description 2
230000002496 gastric effect Effects 0.000 claims description 2
231100001261 hazardous Toxicity 0.000 claims description 2
210000003128 head Anatomy 0.000 claims description 2
150000002611 lead compounds Chemical class 0.000 claims description 2
208000032839 leukemia Diseases 0.000 claims description 2
210000004072 lung Anatomy 0.000 claims description 2
230000001394 metastastic effect Effects 0.000 claims description 2
206010061289 metastatic neoplasm Diseases 0.000 claims description 2
229930014626 natural product Natural products 0.000 claims description 2
210000003739 neck Anatomy 0.000 claims description 2
229940127285 new chemical entity Drugs 0.000 claims description 2
210000001672 ovary Anatomy 0.000 claims description 2
210000000496 pancreas Anatomy 0.000 claims description 2
239000000825 pharmaceutical preparation Substances 0.000 claims description 2
229940127557 pharmaceutical product Drugs 0.000 claims description 2
230000002062 proliferating effect Effects 0.000 claims description 2
210000002307 prostate Anatomy 0.000 claims description 2
239000010865 sewage Substances 0.000 claims description 2
210000003491 skin Anatomy 0.000 claims description 2
210000002784 stomach Anatomy 0.000 claims description 2
241001466804 Carnivora Species 0.000 claims 1
239000012857 radioactive material Substances 0.000 claims 1
230000002068 genetic effect Effects 0.000 abstract description 25
238000001574 biopsy Methods 0.000 abstract description 5
210000001124 body fluid Anatomy 0.000 abstract description 3
239000010839 body fluid Substances 0.000 abstract description 3
239000000872 buffer Substances 0.000 description 31
230000003321 amplification Effects 0.000 description 29
238000003199 nucleic acid amplification method Methods 0.000 description 29
238000004458 analytical method Methods 0.000 description 27
108090000623 proteins and genes Proteins 0.000 description 24
239000000126 substance Substances 0.000 description 22
239000003795 chemical substances by application Substances 0.000 description 19
210000001519 tissue Anatomy 0.000 description 18
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 16
239000000243 solution Substances 0.000 description 16
238000012217 deletion Methods 0.000 description 15
230000037430 deletion Effects 0.000 description 15
239000000463 material Substances 0.000 description 15
238000001514 detection method Methods 0.000 description 14
-1 methylene(methylimino) Chemical class 0.000 description 14
238000006243 chemical reaction Methods 0.000 description 13
230000006870 function Effects 0.000 description 13
HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 12
239000012634 fragment Substances 0.000 description 12
239000011521 glass Substances 0.000 description 12
206010068052 Mosaicism Diseases 0.000 description 11
230000000295 complement effect Effects 0.000 description 11
102000004169 proteins and genes Human genes 0.000 description 11
230000000694 effects Effects 0.000 description 10
238000007639 printing Methods 0.000 description 10
108091032973 (ribonucleotides)n+m Proteins 0.000 description 9
239000012530 fluid Substances 0.000 description 9
108090000765 processed proteins & peptides Proteins 0.000 description 9
241000699670 Mus sp. Species 0.000 description 8
206010012601 diabetes mellitus Diseases 0.000 description 8
238000003780 insertion Methods 0.000 description 8
230000037431 insertion Effects 0.000 description 8
125000003729 nucleotide group Chemical group 0.000 description 8
239000013615 primer Substances 0.000 description 8
230000003595 spectral effect Effects 0.000 description 8
230000027455 binding Effects 0.000 description 7
208000024971 chromosomal disease Diseases 0.000 description 7
230000000052 comparative effect Effects 0.000 description 7
238000003745 diagnosis Methods 0.000 description 7
238000003752 polymerase chain reaction Methods 0.000 description 7
239000013641 positive control Substances 0.000 description 7
150000003839 salts Chemical class 0.000 description 7
230000005945 translocation Effects 0.000 description 7
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 6
208000011359 Chromosome disease Diseases 0.000 description 6
KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 6
ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 6
KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 6
231100000024 genotoxic Toxicity 0.000 description 6
102000004196 processed proteins & peptides Human genes 0.000 description 6
238000013519 translation Methods 0.000 description 6
238000000018 DNA microarray Methods 0.000 description 5
206010056740 Genital discharge Diseases 0.000 description 5
108020004711 Nucleic Acid Probes Proteins 0.000 description 5
108091034117 Oligonucleotide Proteins 0.000 description 5
238000013459 approach Methods 0.000 description 5
239000003153 chemical reaction reagent Substances 0.000 description 5
239000002299 complementary DNA Substances 0.000 description 5
230000029087 digestion Effects 0.000 description 5
GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 5
229920002521 macromolecule Polymers 0.000 description 5
238000004519 manufacturing process Methods 0.000 description 5
238000002493 microarray Methods 0.000 description 5
239000002853 nucleic acid probe Substances 0.000 description 5
239000002773 nucleotide Substances 0.000 description 5
239000008188 pellet Substances 0.000 description 5
229920001184 polypeptide Polymers 0.000 description 5
238000004393 prognosis Methods 0.000 description 5
ANRHNWWPFJCPAZ-UHFFFAOYSA-M thionine Chemical compound [Cl-].C1=CC(N)=CC2=[S+]C3=CC(N)=CC=C3N=C21 ANRHNWWPFJCPAZ-UHFFFAOYSA-M 0.000 description 5
238000011282 treatment Methods 0.000 description 5
YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 4
102000016911 Deoxyribonucleases Human genes 0.000 description 4
108010053770 Deoxyribonucleases Proteins 0.000 description 4
241000196324 Embryophyta Species 0.000 description 4
241000282412 Homo Species 0.000 description 4
102000008299 Nitric Oxide Synthase Human genes 0.000 description 4
108010021487 Nitric Oxide Synthase Proteins 0.000 description 4
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
239000004809 Teflon Substances 0.000 description 4
229920006362 Teflon® Polymers 0.000 description 4
239000012620 biological material Substances 0.000 description 4
230000007547 defect Effects 0.000 description 4
238000000151 deposition Methods 0.000 description 4
230000009395 genetic defect Effects 0.000 description 4
210000005260 human cell Anatomy 0.000 description 4
210000003917 human chromosome Anatomy 0.000 description 4
230000002209 hydrophobic effect Effects 0.000 description 4
230000000670 limiting effect Effects 0.000 description 4
239000003550 marker Substances 0.000 description 4
230000031864 metaphase Effects 0.000 description 4
239000013642 negative control Substances 0.000 description 4
239000004033 plastic Substances 0.000 description 4
229920003023 plastic Polymers 0.000 description 4
229920000642 polymer Polymers 0.000 description 4
239000000047 product Substances 0.000 description 4
238000012216 screening Methods 0.000 description 4
230000035945 sensitivity Effects 0.000 description 4
VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 4
238000003860 storage Methods 0.000 description 4
238000009827 uniform distribution Methods 0.000 description 4
229920000936 Agarose Polymers 0.000 description 3
108091023037 Aptamer Proteins 0.000 description 3
UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
241000588724 Escherichia coli Species 0.000 description 3
PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
108060001084 Luciferase Proteins 0.000 description 3
239000005089 Luciferase Substances 0.000 description 3
239000004677 Nylon Substances 0.000 description 3
108091093037 Peptide nucleic acid Proteins 0.000 description 3
241000700605 Viruses Species 0.000 description 3
210000001766 X chromosome Anatomy 0.000 description 3
JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 3
208000036878 aneuploidy Diseases 0.000 description 3
230000000692 anti-sense effect Effects 0.000 description 3
239000007864 aqueous solution Substances 0.000 description 3
230000015572 biosynthetic process Effects 0.000 description 3
210000003169 central nervous system Anatomy 0.000 description 3
238000010367 cloning Methods 0.000 description 3
230000002559 cytogenic effect Effects 0.000 description 3
238000001962 electrophoresis Methods 0.000 description 3
210000002257 embryonic structure Anatomy 0.000 description 3
238000002474 experimental method Methods 0.000 description 3
230000001605 fetal effect Effects 0.000 description 3
MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 3
238000013467 fragmentation Methods 0.000 description 3
238000006062 fragmentation reaction Methods 0.000 description 3
230000004077 genetic alteration Effects 0.000 description 3
210000000688 human artificial chromosome Anatomy 0.000 description 3
230000006872 improvement Effects 0.000 description 3
238000000338 in vitro Methods 0.000 description 3
229910052751 metal Inorganic materials 0.000 description 3
239000002184 metal Substances 0.000 description 3
YACKEPLHDIMKIO-UHFFFAOYSA-N methylphosphonic acid Chemical compound CP(O)(O)=O YACKEPLHDIMKIO-UHFFFAOYSA-N 0.000 description 3
230000007886 mutagenicity Effects 0.000 description 3
231100000299 mutagenicity Toxicity 0.000 description 3
230000035772 mutation Effects 0.000 description 3
239000002777 nucleoside Substances 0.000 description 3
150000003833 nucleoside derivatives Chemical class 0.000 description 3
229920001778 nylon Polymers 0.000 description 3
239000013612 plasmid Substances 0.000 description 3
229920001296 polysiloxane Polymers 0.000 description 3
239000010453 quartz Substances 0.000 description 3
230000003252 repetitive effect Effects 0.000 description 3
238000011160 research Methods 0.000 description 3
PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 3
238000000926 separation method Methods 0.000 description 3
239000007787 solid Substances 0.000 description 3
239000006228 supernatant Substances 0.000 description 3
238000003786 synthesis reaction Methods 0.000 description 3
238000005406 washing Methods 0.000 description 3
SXGZJKUKBWWHRA-UHFFFAOYSA-N 2-(N-morpholiniumyl)ethanesulfonate Chemical compound [O-]S(=O)(=O)CC[NH+]1CCOCC1 SXGZJKUKBWWHRA-UHFFFAOYSA-N 0.000 description 2
JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 2
DVLFYONBTKHTER-UHFFFAOYSA-N 3-(N-morpholino)propanesulfonic acid Chemical compound OS(=O)(=O)CCCN1CCOCC1 DVLFYONBTKHTER-UHFFFAOYSA-N 0.000 description 2
208000032116 Autoimmune Experimental Encephalomyelitis Diseases 0.000 description 2
241000894006 Bacteria Species 0.000 description 2
102000016928 DNA-directed DNA polymerase Human genes 0.000 description 2
108010014303 DNA-directed DNA polymerase Proteins 0.000 description 2
229920002307 Dextran Polymers 0.000 description 2
102000004190 Enzymes Human genes 0.000 description 2
108090000790 Enzymes Proteins 0.000 description 2
LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 2
241000233866 Fungi Species 0.000 description 2
SEQKRHFRPICQDD-UHFFFAOYSA-N N-tris(hydroxymethyl)methylglycine Chemical compound OCC(CO)(CO)[NH2+]CC([O-])=O SEQKRHFRPICQDD-UHFFFAOYSA-N 0.000 description 2
239000000020 Nitrocellulose Substances 0.000 description 2
ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
239000004698 Polyethylene Substances 0.000 description 2
239000004793 Polystyrene Substances 0.000 description 2
108010066717 Q beta Replicase Proteins 0.000 description 2
108700008625 Reporter Genes Proteins 0.000 description 2
ZSJLQEPLLKMAKR-UHFFFAOYSA-N Streptozotocin Natural products O=NN(C)C(=O)NC1C(O)OC(CO)C(O)C1O ZSJLQEPLLKMAKR-UHFFFAOYSA-N 0.000 description 2
108700019146 Transgenes Proteins 0.000 description 2
208000037280 Trisomy Diseases 0.000 description 2
241000251539 Vertebrata <Metazoa> Species 0.000 description 2
230000002159 abnormal effect Effects 0.000 description 2
239000002253 acid Substances 0.000 description 2
125000003277 amino group Chemical group 0.000 description 2
231100001075 aneuploidy Toxicity 0.000 description 2
238000000137 annealing Methods 0.000 description 2
230000001580 bacterial effect Effects 0.000 description 2
230000037429 base substitution Effects 0.000 description 2
229960002685 biotin Drugs 0.000 description 2
235000020958 biotin Nutrition 0.000 description 2
239000011616 biotin Substances 0.000 description 2
150000001720 carbohydrates Chemical class 0.000 description 2
235000014633 carbohydrates Nutrition 0.000 description 2
238000001444 catalytic combustion detection Methods 0.000 description 2
229920002301 cellulose acetate Polymers 0.000 description 2
238000005119 centrifugation Methods 0.000 description 2
239000000919 ceramic Substances 0.000 description 2
231100000005 chromosome aberration Toxicity 0.000 description 2
230000009089 cytolysis Effects 0.000 description 2
230000001419 dependent effect Effects 0.000 description 2
238000001035 drying Methods 0.000 description 2
201000002491 encephalomyelitis Diseases 0.000 description 2
238000002073 fluorescence micrograph Methods 0.000 description 2
238000002866 fluorescence resonance energy transfer Methods 0.000 description 2
239000013505 freshwater Substances 0.000 description 2
210000004602 germ cell Anatomy 0.000 description 2
PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 2
125000002887 hydroxy group Chemical group [H]O* 0.000 description 2
NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 2
PHTQWCKDNZKARW-UHFFFAOYSA-N isoamylol Chemical compound CC(C)CCO PHTQWCKDNZKARW-UHFFFAOYSA-N 0.000 description 2
239000003446 ligand Substances 0.000 description 2
150000002632 lipids Chemical class 0.000 description 2
230000005291 magnetic effect Effects 0.000 description 2
230000013011 mating Effects 0.000 description 2
108020004999 messenger RNA Proteins 0.000 description 2
150000002739 metals Chemical class 0.000 description 2
MYWUZJCMWCOHBA-VIFPVBQESA-N methamphetamine Chemical compound CN[C@@H](C)CC1=CC=CC=C1 MYWUZJCMWCOHBA-VIFPVBQESA-N 0.000 description 2
229920001220 nitrocellulos Polymers 0.000 description 2
230000009871 nonspecific binding Effects 0.000 description 2
210000004940 nucleus Anatomy 0.000 description 2
210000000287 oocyte Anatomy 0.000 description 2
239000000123 paper Substances 0.000 description 2
239000012188 paraffin wax Substances 0.000 description 2
230000007170 pathology Effects 0.000 description 2
230000002974 pharmacogenomic effect Effects 0.000 description 2
230000000144 pharmacologic effect Effects 0.000 description 2
150000004713 phosphodiesters Chemical class 0.000 description 2
229920000573 polyethylene Polymers 0.000 description 2
229920002223 polystyrene Polymers 0.000 description 2
229920002981 polyvinylidene fluoride Polymers 0.000 description 2
230000035935 pregnancy Effects 0.000 description 2
238000005067 remediation Methods 0.000 description 2
210000003765 sex chromosome Anatomy 0.000 description 2
150000003384 small molecules Chemical class 0.000 description 2
239000011780 sodium chloride Substances 0.000 description 2
238000001228 spectrum Methods 0.000 description 2
238000010561 standard procedure Methods 0.000 description 2
239000011550 stock solution Substances 0.000 description 2
ZSJLQEPLLKMAKR-GKHCUFPYSA-N streptozocin Chemical compound O=NN(C)C(=O)N[C@H]1[C@@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O ZSJLQEPLLKMAKR-GKHCUFPYSA-N 0.000 description 2
229960001052 streptozocin Drugs 0.000 description 2
230000001629 suppression Effects 0.000 description 2
230000002194 synthesizing effect Effects 0.000 description 2
RYYWUUFWQRZTIU-UHFFFAOYSA-K thiophosphate Chemical compound [O-]P([O-])([O-])=S RYYWUUFWQRZTIU-UHFFFAOYSA-K 0.000 description 2
230000002110 toxicologic effect Effects 0.000 description 2
238000013518 transcription Methods 0.000 description 2
230000035897 transcription Effects 0.000 description 2
238000012546 transfer Methods 0.000 description 2
WYTZZXDRDKSJID-UHFFFAOYSA-N (3-aminopropyl)triethoxysilane Chemical compound CCO[Si](OCC)(OCC)CCCN WYTZZXDRDKSJID-UHFFFAOYSA-N 0.000 description 1
BQAZAINZKYOJJV-UHFFFAOYSA-N 1-(2h-isoindol-1-ylmethylidene)isoindole Chemical class N1C=C2C=CC=CC2=C1C=C1C2=CC=CC=C2C=N1 BQAZAINZKYOJJV-UHFFFAOYSA-N 0.000 description 1
PIINGYXNCHTJTF-UHFFFAOYSA-N 2-(2-azaniumylethylamino)acetate Chemical group NCCNCC(O)=O PIINGYXNCHTJTF-UHFFFAOYSA-N 0.000 description 1
QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
SJECZPVISLOESU-UHFFFAOYSA-N 3-trimethoxysilylpropan-1-amine Chemical compound CO[Si](OC)(OC)CCCN SJECZPVISLOESU-UHFFFAOYSA-N 0.000 description 1
CJIJXIFQYOPWTF-UHFFFAOYSA-N 7-hydroxycoumarin Natural products O1C(=O)C=CC2=CC(O)=CC=C21 CJIJXIFQYOPWTF-UHFFFAOYSA-N 0.000 description 1
ORILYTVJVMAKLC-UHFFFAOYSA-N Adamantane Natural products C1C(C2)CC3CC1CC2C3 ORILYTVJVMAKLC-UHFFFAOYSA-N 0.000 description 1
108010000239 Aequorin Proteins 0.000 description 1
108010088751 Albumins Proteins 0.000 description 1
102000009027 Albumins Human genes 0.000 description 1
102000002260 Alkaline Phosphatase Human genes 0.000 description 1
108020004774 Alkaline Phosphatase Proteins 0.000 description 1
108700028369 Alleles Proteins 0.000 description 1
JBRZTFJDHDCESZ-UHFFFAOYSA-N AsGa Chemical compound [As]#[Ga] JBRZTFJDHDCESZ-UHFFFAOYSA-N 0.000 description 1
206010003445 Ascites Diseases 0.000 description 1
235000018185 Betula X alpestris Nutrition 0.000 description 1
235000018212 Betula X uliginosa Nutrition 0.000 description 1
ZOXJGFHDIHLPTG-UHFFFAOYSA-N Boron Chemical compound [B] ZOXJGFHDIHLPTG-UHFFFAOYSA-N 0.000 description 1
FERIUCNNQQJTOY-UHFFFAOYSA-M Butyrate Chemical compound CCCC([O-])=O FERIUCNNQQJTOY-UHFFFAOYSA-M 0.000 description 1
FERIUCNNQQJTOY-UHFFFAOYSA-N Butyric acid Natural products CCCC(O)=O FERIUCNNQQJTOY-UHFFFAOYSA-N 0.000 description 1
241000700198 Cavia Species 0.000 description 1
108020004635 Complementary DNA Proteins 0.000 description 1
206010010356 Congenital anomaly Diseases 0.000 description 1
229920002261 Corn starch Polymers 0.000 description 1
241000192700 Cyanobacteria Species 0.000 description 1
IGXWBGJHJZYPQS-SSDOTTSWSA-N D-Luciferin Chemical compound OC(=O)[C@H]1CSC(C=2SC3=CC=C(O)C=C3N=2)=N1 IGXWBGJHJZYPQS-SSDOTTSWSA-N 0.000 description 1
102000053602 DNA Human genes 0.000 description 1
102000004594 DNA Polymerase I Human genes 0.000 description 1
108010017826 DNA Polymerase I Proteins 0.000 description 1
230000005778 DNA damage Effects 0.000 description 1
231100000277 DNA damage Toxicity 0.000 description 1
239000003155 DNA primer Substances 0.000 description 1
XPDXVDYUQZHFPV-UHFFFAOYSA-N Dansyl Chloride Chemical compound C1=CC=C2C(N(C)C)=CC=CC2=C1S(Cl)(=O)=O XPDXVDYUQZHFPV-UHFFFAOYSA-N 0.000 description 1
CYCGRDQQIOGCKX-UHFFFAOYSA-N Dehydro-luciferin Natural products OC(=O)C1=CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 CYCGRDQQIOGCKX-UHFFFAOYSA-N 0.000 description 1
208000016192 Demyelinating disease Diseases 0.000 description 1
206010012305 Demyelination Diseases 0.000 description 1
102000007260 Deoxyribonuclease I Human genes 0.000 description 1
108010008532 Deoxyribonuclease I Proteins 0.000 description 1
SHIBSTMRCDJXLN-UHFFFAOYSA-N Digoxigenin Natural products C1CC(C2C(C3(C)CCC(O)CC3CC2)CC2O)(O)C2(C)C1C1=CC(=O)OC1 SHIBSTMRCDJXLN-UHFFFAOYSA-N 0.000 description 1
238000002965 ELISA Methods 0.000 description 1
108010067770 Endopeptidase K Proteins 0.000 description 1
241000283070 Equus zebra Species 0.000 description 1
BJGNCJDXODQBOB-UHFFFAOYSA-N Fivefly Luciferin Natural products OC(=O)C1CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 BJGNCJDXODQBOB-UHFFFAOYSA-N 0.000 description 1
102000020897 Formins Human genes 0.000 description 1
108091022623 Formins Proteins 0.000 description 1
229940123457 Free radical scavenger Drugs 0.000 description 1
229910001218 Gallium arsenide Inorganic materials 0.000 description 1
108010010803 Gelatin Proteins 0.000 description 1
108010043121 Green Fluorescent Proteins Proteins 0.000 description 1
239000007995 HEPES buffer Substances 0.000 description 1
208000028782 Hereditary disease Diseases 0.000 description 1
108010001336 Horseradish Peroxidase Proteins 0.000 description 1
102000004877 Insulin Human genes 0.000 description 1
108090001061 Insulin Proteins 0.000 description 1
ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 1
ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 1
DDWFXDSYGUXRAY-UHFFFAOYSA-N Luciferin Natural products CCc1c(C)c(CC2NC(=O)C(=C2C=C)C)[nH]c1Cc3[nH]c4C(=C5/NC(CC(=O)O)C(C)C5CC(=O)O)CC(=O)c4c3C DDWFXDSYGUXRAY-UHFFFAOYSA-N 0.000 description 1
239000007993 MOPS buffer Substances 0.000 description 1
241000013355 Mycteroperca interstitialis Species 0.000 description 1
102000006386 Myelin Proteins Human genes 0.000 description 1
108010083674 Myelin Proteins Proteins 0.000 description 1
108010004729 Phycoerythrin Proteins 0.000 description 1
239000002202 Polyethylene glycol Substances 0.000 description 1
208000020584 Polyploidy Diseases 0.000 description 1
239000004743 Polypropylene Substances 0.000 description 1
229920000297 Rayon Polymers 0.000 description 1
102000006382 Ribonucleases Human genes 0.000 description 1
108010083644 Ribonucleases Proteins 0.000 description 1
108091028664 Ribonucleotide Proteins 0.000 description 1
240000004808 Saccharomyces cerevisiae Species 0.000 description 1
108020004487 Satellite DNA Proteins 0.000 description 1
BLRPTPMANUNPDV-UHFFFAOYSA-N Silane Chemical compound [SiH4] BLRPTPMANUNPDV-UHFFFAOYSA-N 0.000 description 1
239000004965 Silica aerogel Substances 0.000 description 1
229920002472 Starch Polymers 0.000 description 1
206010043275 Teratogenicity Diseases 0.000 description 1
241000269368 Xenopus laevis Species 0.000 description 1
210000002593 Y chromosome Anatomy 0.000 description 1
OYVGFASYJHUBSP-YGOYTEALSA-N [[(2r,3s,5r)-5-(4,5-diamino-2-oxopyrimidin-1-yl)-3-hydroxy-5-prop-2-ynyloxolan-2-yl]methoxy-hydroxyphosphoryl] phosphono hydrogen phosphate Chemical compound O=C1N=C(N)C(N)=CN1[C@]1(CC#C)O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C1 OYVGFASYJHUBSP-YGOYTEALSA-N 0.000 description 1
230000001594 aberrant effect Effects 0.000 description 1
208000037919 acquired disease Diseases 0.000 description 1
125000000217 alkyl group Chemical group 0.000 description 1
150000001413 amino acids Chemical class 0.000 description 1
230000003322 aneuploid effect Effects 0.000 description 1
210000004102 animal cell Anatomy 0.000 description 1
239000003963 antioxidant agent Substances 0.000 description 1
238000003556 assay Methods 0.000 description 1
238000011888 autopsy Methods 0.000 description 1
239000011324 bead Substances 0.000 description 1
OGBVRMYSNSKIEF-UHFFFAOYSA-L benzyl-dioxido-oxo-$l^{5}-phosphane Chemical compound [O-]P([O-])(=O)CC1=CC=CC=C1 OGBVRMYSNSKIEF-UHFFFAOYSA-L 0.000 description 1
230000003115 biocidal effect Effects 0.000 description 1
239000013060 biological fluid Substances 0.000 description 1
239000012472 biological sample Substances 0.000 description 1
230000000903 blocking effect Effects 0.000 description 1
210000000601 blood cell Anatomy 0.000 description 1
238000009583 bone marrow aspiration Methods 0.000 description 1
229910052796 boron Inorganic materials 0.000 description 1
244000309464 bull Species 0.000 description 1
238000005251 capillar electrophoresis Methods 0.000 description 1
125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
230000001413 cellular effect Effects 0.000 description 1
229920002678 cellulose Polymers 0.000 description 1
239000001913 cellulose Substances 0.000 description 1
230000008859 change Effects 0.000 description 1
239000013043 chemical agent Substances 0.000 description 1
210000004252 chorionic villi Anatomy 0.000 description 1
239000002131 composite material Substances 0.000 description 1
238000004590 computer program Methods 0.000 description 1
238000010276 construction Methods 0.000 description 1
239000013068 control sample Substances 0.000 description 1
239000008120 corn starch Substances 0.000 description 1
230000008878 coupling Effects 0.000 description 1
238000010168 coupling process Methods 0.000 description 1
238000005859 coupling reaction Methods 0.000 description 1
244000038559 crop plants Species 0.000 description 1
238000012136 culture method Methods 0.000 description 1
229940104302 cytosine Drugs 0.000 description 1
RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 description 1
238000007405 data analysis Methods 0.000 description 1
230000003247 decreasing effect Effects 0.000 description 1
230000002950 deficient Effects 0.000 description 1
230000003111 delayed effect Effects 0.000 description 1
230000002939 deleterious effect Effects 0.000 description 1
239000005547 deoxyribonucleotide Substances 0.000 description 1
125000002637 deoxyribonucleotide group Chemical group 0.000 description 1
230000008021 deposition Effects 0.000 description 1
238000011161 development Methods 0.000 description 1
QONQRTHLHBTMGP-UHFFFAOYSA-N digitoxigenin Natural products CC12CCC(C3(CCC(O)CC3CC3)C)C3C11OC1CC2C1=CC(=O)OC1 QONQRTHLHBTMGP-UHFFFAOYSA-N 0.000 description 1
SHIBSTMRCDJXLN-KCZCNTNESA-N digoxigenin Chemical compound C1([C@@H]2[C@@]3([C@@](CC2)(O)[C@H]2[C@@H]([C@@]4(C)CC[C@H](O)C[C@H]4CC2)C[C@H]3O)C)=CC(=O)OC1 SHIBSTMRCDJXLN-KCZCNTNESA-N 0.000 description 1
238000010790 dilution Methods 0.000 description 1
239000012895 dilution Substances 0.000 description 1
NAGJZTKCGNOGPW-UHFFFAOYSA-K dioxido-sulfanylidene-sulfido-$l^{5}-phosphane Chemical compound [O-]P([O-])([S-])=S NAGJZTKCGNOGPW-UHFFFAOYSA-K 0.000 description 1
OVTCUIZCVUGJHS-UHFFFAOYSA-N dipyrrin Chemical compound C=1C=CNC=1C=C1C=CC=N1 OVTCUIZCVUGJHS-UHFFFAOYSA-N 0.000 description 1
239000012153 distilled water Substances 0.000 description 1
238000009826 distribution Methods 0.000 description 1
231100000673 dose–response relationship Toxicity 0.000 description 1
239000003814 drug Substances 0.000 description 1
238000010828 elution Methods 0.000 description 1
210000002308 embryonic cell Anatomy 0.000 description 1
230000003511 endothelial effect Effects 0.000 description 1
238000005516 engineering process Methods 0.000 description 1
230000006862 enzymatic digestion Effects 0.000 description 1
230000002255 enzymatic effect Effects 0.000 description 1
238000012869 ethanol precipitation Methods 0.000 description 1
238000001704 evaporation Methods 0.000 description 1
230000008020 evaporation Effects 0.000 description 1
238000000605 extraction Methods 0.000 description 1
230000002550 fecal effect Effects 0.000 description 1
230000004720 fertilization Effects 0.000 description 1
210000003754 fetus Anatomy 0.000 description 1
125000000524 functional group Chemical group 0.000 description 1
239000005350 fused silica glass Substances 0.000 description 1
239000000499 gel Substances 0.000 description 1
229920000159 gelatin Polymers 0.000 description 1
235000019322 gelatine Nutrition 0.000 description 1
235000011852 gelatine desserts Nutrition 0.000 description 1
238000012252 genetic analysis Methods 0.000 description 1
239000010931 gold Substances 0.000 description 1
229910052737 gold Inorganic materials 0.000 description 1
244000144993 groups of animals Species 0.000 description 1
230000003394 haemopoietic effect Effects 0.000 description 1
210000004090 human X chromosome Anatomy 0.000 description 1
229910052739 hydrogen Inorganic materials 0.000 description 1
239000001257 hydrogen Substances 0.000 description 1
WGCNASOHLSPBMP-UHFFFAOYSA-N hydroxyacetaldehyde Natural products OCC=O WGCNASOHLSPBMP-UHFFFAOYSA-N 0.000 description 1
230000000984 immunochemical effect Effects 0.000 description 1
238000007901 in situ hybridization Methods 0.000 description 1
238000010348 incorporation Methods 0.000 description 1
238000011534 incubation Methods 0.000 description 1
230000006698 induction Effects 0.000 description 1
230000002401 inhibitory effect Effects 0.000 description 1
229910001410 inorganic ion Inorganic materials 0.000 description 1
229940125396 insulin Drugs 0.000 description 1
230000003993 interaction Effects 0.000 description 1
238000007912 intraperitoneal administration Methods 0.000 description 1
238000007913 intrathecal administration Methods 0.000 description 1
238000001990 intravenous administration Methods 0.000 description 1
150000002500 ions Chemical class 0.000 description 1
238000002955 isolation Methods 0.000 description 1
238000011005 laboratory method Methods 0.000 description 1
101150066555 lacZ gene Proteins 0.000 description 1
229910052747 lanthanoid Inorganic materials 0.000 description 1
150000002602 lanthanoids Chemical class 0.000 description 1
229920000126 latex Polymers 0.000 description 1
239000004816 latex Substances 0.000 description 1
238000007834 ligase chain reaction Methods 0.000 description 1
239000007788 liquid Substances 0.000 description 1
244000144972 livestock Species 0.000 description 1
HWYHZTIRURJOHG-UHFFFAOYSA-N luminol Chemical compound O=C1NNC(=O)C2=C1C(N)=CC=C2 HWYHZTIRURJOHG-UHFFFAOYSA-N 0.000 description 1
210000004698 lymphocyte Anatomy 0.000 description 1
239000006166 lysate Substances 0.000 description 1
230000036244 malformation Effects 0.000 description 1
210000001161 mammalian embryo Anatomy 0.000 description 1
238000001840 matrix-assisted laser desorption--ionisation time-of-flight mass spectrometry Methods 0.000 description 1
230000001404 mediated effect Effects 0.000 description 1
238000002844 melting Methods 0.000 description 1
230000008018 melting Effects 0.000 description 1
210000004379 membrane Anatomy 0.000 description 1
239000012528 membrane Substances 0.000 description 1
DZVCFNFOPIZQKX-LTHRDKTGSA-M merocyanine Chemical compound [Na+].O=C1N(CCCC)C(=O)N(CCCC)C(=O)C1=C\C=C\C=C/1N(CCCS([O-])(=O)=O)C2=CC=CC=C2O\1 DZVCFNFOPIZQKX-LTHRDKTGSA-M 0.000 description 1
229910052752 metalloid Inorganic materials 0.000 description 1
150000002738 metalloids Chemical class 0.000 description 1
244000005700 microbiome Species 0.000 description 1
238000000520 microinjection Methods 0.000 description 1
239000011859 microparticle Substances 0.000 description 1
230000011278 mitosis Effects 0.000 description 1
238000002156 mixing Methods 0.000 description 1
238000012986 modification Methods 0.000 description 1
230000004048 modification Effects 0.000 description 1
238000010369 molecular cloning Methods 0.000 description 1
238000012544 monitoring process Methods 0.000 description 1
208000030454 monosomy Diseases 0.000 description 1
XTGGILXPEMRCFM-UHFFFAOYSA-N morpholin-4-yl carbamate Chemical compound NC(=O)ON1CCOCC1 XTGGILXPEMRCFM-UHFFFAOYSA-N 0.000 description 1
238000010172 mouse model Methods 0.000 description 1
201000006417 multiple sclerosis Diseases 0.000 description 1
230000000869 mutational effect Effects 0.000 description 1
210000005012 myelin Anatomy 0.000 description 1
ZTLGJPIZUOVDMT-UHFFFAOYSA-N n,n-dichlorotriazin-4-amine Chemical compound ClN(Cl)C1=CC=NN=N1 ZTLGJPIZUOVDMT-UHFFFAOYSA-N 0.000 description 1
238000013188 needle biopsy Methods 0.000 description 1
238000007899 nucleic acid hybridization Methods 0.000 description 1
238000011330 nucleic acid test Methods 0.000 description 1
229940046166 oligodeoxynucleotide Drugs 0.000 description 1
229940124276 oligodeoxyribonucleotide Drugs 0.000 description 1
238000002966 oligonucleotide array Methods 0.000 description 1
238000005457 optimization Methods 0.000 description 1
210000000056 organ Anatomy 0.000 description 1
230000003204 osmotic effect Effects 0.000 description 1
230000002611 ovarian Effects 0.000 description 1
230000003647 oxidation Effects 0.000 description 1
238000007254 oxidation reaction Methods 0.000 description 1
230000005298 paramagnetic effect Effects 0.000 description 1
NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
229940085991 phosphate ion Drugs 0.000 description 1
PTMHPRAIXMAOOB-UHFFFAOYSA-L phosphoramidate Chemical compound NP([O-])([O-])=O PTMHPRAIXMAOOB-UHFFFAOYSA-L 0.000 description 1
IEQIEDJGQAUEQZ-UHFFFAOYSA-N phthalocyanine Chemical compound N1C(N=C2C3=CC=CC=C3C(N=C3C4=CC=CC=C4C(=N4)N3)=N2)=C(C=CC=C2)C2=C1N=C1C2=CC=CC=C2C4=N1 IEQIEDJGQAUEQZ-UHFFFAOYSA-N 0.000 description 1
230000035479 physiological effects, processes and functions Effects 0.000 description 1
229920002401 polyacrylamide Polymers 0.000 description 1
229920001223 polyethylene glycol Polymers 0.000 description 1
229920000139 polyethylene terephthalate Polymers 0.000 description 1
239000005020 polyethylene terephthalate Substances 0.000 description 1
229920000193 polymethacrylate Polymers 0.000 description 1
229920006324 polyoxymethylene Polymers 0.000 description 1
229920001155 polypropylene Polymers 0.000 description 1
239000011148 porous material Substances 0.000 description 1
239000002244 precipitate Substances 0.000 description 1
244000062645 predators Species 0.000 description 1
239000002987 primer (paints) Substances 0.000 description 1
230000008569 process Effects 0.000 description 1
238000007388 punch biopsy Methods 0.000 description 1
238000006862 quantum yield reaction Methods 0.000 description 1
238000010791 quenching Methods 0.000 description 1
239000002516 radical scavenger Substances 0.000 description 1
230000002285 radioactive effect Effects 0.000 description 1
239000002964 rayon Substances 0.000 description 1
239000011541 reaction mixture Substances 0.000 description 1
230000008707 rearrangement Effects 0.000 description 1
238000003259 recombinant expression Methods 0.000 description 1
238000010188 recombinant method Methods 0.000 description 1
230000002829 reductive effect Effects 0.000 description 1
239000013643 reference control Substances 0.000 description 1
230000010076 replication Effects 0.000 description 1
230000004044 response Effects 0.000 description 1
108091008146 restriction endonucleases Proteins 0.000 description 1
230000002441 reversible effect Effects 0.000 description 1
239000001022 rhodamine dye Substances 0.000 description 1
239000002336 ribonucleotide Substances 0.000 description 1
125000002652 ribonucleotide group Chemical group 0.000 description 1
210000003296 saliva Anatomy 0.000 description 1
229920006395 saturated elastomer Polymers 0.000 description 1
238000012163 sequencing technique Methods 0.000 description 1
210000002966 serum Anatomy 0.000 description 1
238000010008 shearing Methods 0.000 description 1
238000007873 sieving Methods 0.000 description 1
229910000077 silane Inorganic materials 0.000 description 1
238000004513 sizing Methods 0.000 description 1
239000002904 solvent Substances 0.000 description 1
230000000392 somatic effect Effects 0.000 description 1
238000000527 sonication Methods 0.000 description 1
125000006850 spacer group Chemical group 0.000 description 1
230000009870 specific binding Effects 0.000 description 1
208000000995 spontaneous abortion Diseases 0.000 description 1
235000019698 starch Nutrition 0.000 description 1
239000008223 sterile water Substances 0.000 description 1
125000005504 styryl group Chemical group 0.000 description 1
238000007920 subcutaneous administration Methods 0.000 description 1
235000000346 sugar Nutrition 0.000 description 1
150000008163 sugars Chemical class 0.000 description 1
IIACRCGMVDHOTQ-UHFFFAOYSA-M sulfamate Chemical compound NS([O-])(=O)=O IIACRCGMVDHOTQ-UHFFFAOYSA-M 0.000 description 1
239000013589 supplement Substances 0.000 description 1
230000004083 survival effect Effects 0.000 description 1
230000002459 sustained effect Effects 0.000 description 1
208000024891 symptom Diseases 0.000 description 1
208000011580 syndromic disease Diseases 0.000 description 1
238000010189 synthetic method Methods 0.000 description 1
229920001059 synthetic polymer Polymers 0.000 description 1
235000012976 tarts Nutrition 0.000 description 1
210000001138 tear Anatomy 0.000 description 1
231100000211 teratogenicity Toxicity 0.000 description 1
230000002381 testicular Effects 0.000 description 1
WGTODYJZXSJIAG-UHFFFAOYSA-N tetramethylrhodamine chloride Chemical compound [Cl-].C=12C=CC(N(C)C)=CC2=[O+]C2=CC(N(C)C)=CC=C2C=1C1=CC=CC=C1C(O)=O WGTODYJZXSJIAG-UHFFFAOYSA-N 0.000 description 1
MPLHNVLQVRSVEE-UHFFFAOYSA-N texas red Chemical compound [O-]S(=O)(=O)C1=CC(S(Cl)(=O)=O)=CC=C1C(C1=CC=2CCCN3CCCC(C=23)=C1O1)=C2C1=C(CCC1)C3=[N+]1CCCC3=C2 MPLHNVLQVRSVEE-UHFFFAOYSA-N 0.000 description 1
231100000041 toxicology testing Toxicity 0.000 description 1
108091006106 transcriptional activators Proteins 0.000 description 1
238000011830 transgenic mouse model Methods 0.000 description 1
210000005239 tubule Anatomy 0.000 description 1
210000004881 tumor cell Anatomy 0.000 description 1
HFTAFOQKODTIJY-UHFFFAOYSA-N umbelliferone Natural products Cc1cc2C=CC(=O)Oc2cc1OCC=CC(C)(C)O HFTAFOQKODTIJY-UHFFFAOYSA-N 0.000 description 1
ORHBXUUXSCNDEV-UHFFFAOYSA-N umbelliferone Chemical compound C1=CC(=O)OC2=CC(O)=CC=C21 ORHBXUUXSCNDEV-UHFFFAOYSA-N 0.000 description 1
241001515965 unidentified phage Species 0.000 description 1
210000002700 urine Anatomy 0.000 description 1
229920002554 vinyl polymer Polymers 0.000 description 1
230000000007 visual effect Effects 0.000 description 1
230000036266 weeks of gestation Effects 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers

Landscapes

Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Life Sciences & Earth Sciences (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Microbiology (AREA)
Biochemistry (AREA)
Physics & Mathematics (AREA)
Molecular Biology (AREA)
Biotechnology (AREA)
Biophysics (AREA)
Analytical Chemistry (AREA)
Immunology (AREA)
Bioinformatics & Cheminformatics (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Apparatus Associated With Microorganisms And Enzymes (AREA)

CA002534441A 2003-08-01 2004-08-02 Jeux ordonnes d'echantillons, procedes et kits de preparation et d'utilisation de jeux ordonnes d'echantillons genomiques synteniques pour l'etablissement de diagnostics et la toxicologie Abandoned CA2534441A1 (fr)

Applications Claiming Priority (3)

Application Number	Priority Date	Filing Date	Title
US49195603P	2003-08-01	2003-08-01
US60/491,956		2003-08-01
PCT/US2004/025124 WO2005012500A2 (fr)	2003-08-01	2004-08-02	Jeux ordonnes d'echantillons, procedes et kits de preparation et d'utilisation de jeux ordonnes d'echantillons genomiques synteniques pour l'etablissement de diagnostics et la toxicologie

Publications (1)

Publication Number	Publication Date
CA2534441A1 true CA2534441A1 (fr)	2005-02-10

Family

ID=34115573

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CA002534441A Abandoned CA2534441A1 (fr)	2003-08-01	2004-08-02	Jeux ordonnes d'echantillons, procedes et kits de preparation et d'utilisation de jeux ordonnes d'echantillons genomiques synteniques pour l'etablissement de diagnostics et la toxicologie

Country Status (5)

Country	Link
EP (1)	EP1664273A4 (fr)
JP (1)	JP2007521014A (fr)
AU (1)	AU2004262040A1 (fr)
CA (1)	CA2534441A1 (fr)
WO (1)	WO2005012500A2 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
EP2876168B1 (fr) *	2009-12-29	2018-05-30	Cancer Genetics, Inc.	Puce d'adn et procede pour le diagnostic et le pronostic de lymphomes b matures
BR112020023025A2 (pt) *	2018-05-11	2021-03-09	Heineken Supply Chain B.V.	Identificação de produtos raros do cruzamento de organismos
CN113917130A (zh) *	2021-10-11	2022-01-11	青蛙王子（福建）婴童护理用品有限公司	利用斑马鱼评价婴童化妆品眼刺激的方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
ATE205542T1 (de) *	1992-03-04	2001-09-15	Univ California	Vergleichende genomhybridisierung
WO2001048242A2 (fr) *	1999-12-29	2001-07-05	Mergen Ltd.	Procedes d'amplification et de detection de plusieurs polynucleotides sur un support en phase solide
WO2002095065A2 (fr) *	2001-05-18	2002-11-28	Azign Bioscience A/S	Matrices de recepteurs couples a la proteine g

2004
- 2004-08-02 WO PCT/US2004/025124 patent/WO2005012500A2/fr not_active Ceased
- 2004-08-02 CA CA002534441A patent/CA2534441A1/fr not_active Abandoned
- 2004-08-02 AU AU2004262040A patent/AU2004262040A1/en not_active Abandoned
- 2004-08-02 JP JP2006522687A patent/JP2007521014A/ja active Pending
- 2004-08-02 EP EP04780031A patent/EP1664273A4/fr not_active Withdrawn

Also Published As

Publication number	Publication date
WO2005012500A3 (fr)	2007-05-31
EP1664273A4 (fr)	2009-04-01
EP1664273A2 (fr)	2006-06-07
AU2004262040A1 (en)	2005-02-10
JP2007521014A (ja)	2007-08-02
WO2005012500A2 (fr)	2005-02-10

Legal Events

Date	Code	Title	Description
2009-04-30	FZDE	Discontinued

Publication	Publication Date	Title
JP7028807B2 (ja)	2022-03-02	ゲノムアセンブリ及びハプロタイプフェージングの方法
AU2002330141B2 (en)	2007-11-22	Methods for detecting genetic mosaicisms using arrays
JP5579999B2 (ja)	2014-08-27	ヒト第２０染色体の２０ｑ１３領域における新規アンプリコンおよびその使用
EP2121977B1 (fr)	2017-06-21	Capture des chromosomes avec une conformation circulaire
AU2002330141A1 (en)	2003-06-26	Methods for detecting genetic mosaicisms using arrays
JP2005519634A (ja)	2005-07-07	核酸組成物及びアレイ及びそれらを用いる方法
US7439346B2 (en)	2008-10-21	Nucleic acids arrays and methods of use therefor
JP2009519710A (ja)	2009-05-21	遺伝子発現調節エレメントのハイスループットでの特徴付けのための機能性アレイ
CA2534441A1 (fr)	2005-02-10	Jeux ordonnes d'echantillons, procedes et kits de preparation et d'utilisation de jeux ordonnes d'echantillons genomiques synteniques pour l'etablissement de diagnostics et la toxicologie
US20030124542A1 (en)	2003-07-03	Methods for mapping the chromosomal loci of genes expressed by a cell
US20250154576A1 (en)	2025-05-15	Genotyping of targeted loci with single-cell chromatin accessibility
US20090075841A1 (en)	2009-03-19	Nucleic acids arrays and methods of use therefor
US6867035B2 (en)	2005-03-15	Cell libraries indexed to nucleic acid microarrays
EP1295951A1 (fr)	2003-03-26	Utlilisation des bibliothèques de cellules
Class et al.	2010	Inventors: Chao-Ting Wu (Brookline, MA, US) Chao-Ting Wu (Brookline, MA, US) George M. Church (Brookline, MA, US) Benjamin Richard Williams (Seattle, WA, US) Assignees: President and Fellows of Havard College
CA2350976A1 (fr)	2001-12-16	Bibliotheques de cellules indexees en fonction de jeux ordonnes de microechantillons d'acides nucleiques